miR-146a rs2431697 identifies myeloproliferative neoplasm patients with higher secondary myelofibrosis progression risk

Myelofibrosis (MF) occurs as part of the natural history of polycythemia vera (PV) and essential thrombocythemia (ET), and remarkably shortens survival. Although JAK2V617F and CALR allele burden are the main transformation risk factors, inflammation plays a critical role by driving clonal expansion toward end-stage disease. NF-κB is a key mediator of inflammation-induced carcinogenesis. Here, we explored the involvement of miR-146a, a brake in NF-κB signaling, in MPN susceptibility and progression. rs2910164 and rs2431697, that affect miR-146a expression, were analyzed in 967 MPN (320 PV/333 ET/314 MF) patients and 600 controls. We found that rs2431697 TT genotype was associated with MF, particularly with post-PV/ET MF (HR = 1.5; p < 0.05). Among 232 PV/ET patients (follow-up time=8.5 years), 18 (7.8%) progressed to MF, being MF-free-survival shorter for rs2431697 TT than CC + CT patients (p = 0.01). Multivariate analysis identified TT genotype as independent predictor of MF progression. In addition, TT (vs. CC + CT) patients showed increased plasma inflammatory cytokines. Finally, miR-146a−/− mice showed significantly higher Stat3 activity with aging, parallel to the development of the MF-like phenotype. In conclusion, we demonstrated that rs2431697 TT genotype is an early predictor of MF progression independent of the JAK2V617F allele burden. Low levels of miR-146a contribute to the MF phenotype by increasing Stat3 signaling

Atributos morfológicos y fisiológicos de genotipos de Brachiaria en un suelo con bajo fósforo disponible y alta saturación de aluminio = Morphologic and physiological attributes of Brachiaria genotypes in soil with low available phosphorus and high aluminum saturation

Se evaluaron en invernadero en un suelo de Matazul, Meta, Colombia durante 6 semanas, 2 genotipos de Brachiaria (uno mejor adaptado a bajo P, B. decumbens y otro menos adaptado, B. ruziziensis) y 8 progenies del cruzamiento entre ellos ( mejores adaptados: H-7, H-40, H-28, H-58; menos adaptados: H-190, H-94, H-82, H-179). Los genotipos mejor adaptados presentaron mayor biomasa aérea, explicada por mayor área foliar, mayor absorción de P en el tallo y mayor volumen de raíz. Las características morfológicas de las raíces, principalmente longitud, peso seco y contenido de P, permiteron mejor adaptación a bajo P disponible en el suelo. El periodo de evaluación fue suficiente para observar diferencias entre progenies. = Two Brachiaria genotypes (Brachiaria decumbens, considered as better adapted and Brachiaria ruziziensis, considered as less adapted) and 8 progenies from the cross of these two (H-7, H-40, H-28 and H-58 more adapted; H-190, H-94, H-82 and H-179 less adapted) were evaluated during 6 weeks to detect differences in shoot and root attributes when grown in an acid soil from Matazul, Meta, Colombia. Results indicated that better adapted genotypes to low P had more shoot biomass, this could be explained by a greater leaf area, more P uptake in the stem and greater root volume. The root attributes, root length, root dry weight and root P content contributed to greater adaptation to low P availability in acid soils. Six weeks of plant growth was not adequate time to distinguish differences in adaptation to low P among the two parents, but it was adequate time to identify superior progenies from the crosses

Foraging behaviour of honey bees (Apis mellifera L.) on transgenic oilseed rape (Brassica napus L. var. oleifera)

International audienc