9 research outputs found
Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA
Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes
Medical education and mental health during COVID-19: a survey across 9 countries
Objectives: To investigate students' experience with medical education alongside their mental and physical health since the onset of the COVID-19 pandemic across nine countries. Methods: A cross-sectional online survey was distributed by local collaborators to 2,280 medical students across 148 medical schools in Brazil, Chile, Colombia, Germany, Italy, Japan, Mexico, Spain, and Venezuela using non-probability convenience sampling from June 22 to July 24, 2020. Students answered questions regarding teaching, internet use, COVID-19, physical and mental well-being. A multivariate logistic regression examined factors associated with depressed mood, insomnia, and headache. Results: Academic teaching shifted to a virtual (67%, n=1,534) or hybrid environment (23%, n=531), whilst bedside teaching was suspended or cancelled (93%, n=2,120). Across all countries students were equally satisfied with the teaching modality, quantity, quality, and the evaluation system of in-person, hybrid, and online curricula. Negative changes in mental (40% (n=912) insomnia, 57% (n=1,300) emotional irritability, 47% (n=1,072) emotional instability, 41% (n=935) anhedonia, 40% (n=912) depressed mood) and physical (36% (n=821) headache, 57% (n=1,299) ocular tiredness, 49% (n=1,117) backache) health symptoms were frequently observed. Positive associations between the number of daily screen hours and depressed mood (adjusted odds ratio (AOR)=1.09, 95%CI: 1.05-1.12, p<.001), insomnia (AOR=1.08, 95%CI: 1.05-1.11, p<.001), and headache (AOR=1.11, 95%CI: 1.07-1.14, p<.001) were identified. Conclusions: Students' experience with digital and hybrid medical curricula was diverse during the pandemic. Education modality, quantity, and quality were positively evaluated. However, students' mental and physical health worsened. Besides bedside teaching, faculties ought to digitalize and strengthen social communities and extend support services for students
Social Anxiety and Alcohol-Related Impairment: The Mediational Impact of Solitary Drinking
Social anxiety disorder more than quadruples the risk of developing an alcohol use disorder, yet
it is inconsistently linked to drinking frequency. Inconsistent findings may be at least partially
due to lack of attention to drinking context – it may be that socially anxious individuals are
especially vulnerable to drinking more often in specific contexts that increase their risk for
alcohol-related problems. For instance, socially anxious persons may drink more often while
alone, before social situations for “liquid courage” and/or after social situations to manage
negative thoughts about their performance. Among current (past-month) drinkers (N = 776),
social anxiety was significantly, positively related to solitary drinking frequency and was
negatively related to social drinking frequency. Social anxiety was indirectly (via solitary drinking
frequency) related to greater past-month drinking frequency and more drinking-related
problems. Social anxiety was also indirectly (via social drinking frequency) negatively related to
past-month drinking frequency and drinking-related problems. Findings suggest that socially
anxious persons may be vulnerable to more frequent drinking in particular contexts (in this case
alone) and that this context-specific drinking may play an important role in drinking problems
among these high-risk individuals
Anxiety Sensitivity: An Examination of the Relationship With Authoritarian, Authoritative, and Permissive Parental Styles
A substantial literature has investigated the role of parenting on a child's development. Several classifications of parenting styles (i.e., permissive, authoritarian, authoritative) have been linked to a wide range of negative outcomes such as mood and anxiety problems; however, their respective associations to anxiety sensitivity (AS) remain unclear. Using a nonclinical sample of young adults (N = 227), this study is the first to empirically investigate whether parenting styles were differentially associated with AS, controlling for general depression and anxiety symptoms. Hierarchical linear regression analyses showed that authoritarian and permissive styles were associated with elevated AS. Permissive parenting was associated with the AS physical subfactor, whereas authoritarian parenting was associated with the AS social subfactor. Moreover, AS was found to mediate the relationship between specific parental styles and anxiety symptoms as well as depressive symptoms. Findings suggest that AS may mediate the relationship between parenting styles and negative psychological outcomes
Prevalence and distribution of antimicrobial resistance in effluent wastewater from animal slaughter facilities: A systematic review
The extensive use of antibiotics in food animal production and disposal of untreated wastewater from food animal slaughter facilities may create a shift in microbiomes of different ecosystems by generating reservoirs of antimicrobial resistance along the human-animal-environmental interface. This epidemiological problem has
been studied, but its magnitude and impact on a global scale is poorly characterised. A systematic review was done to determine global prevalence and distribution patterns of antimicrobial resistance in effluent wastewater from animal slaughter facilities. Extracted data were stratified into rational groups for secondary analyses and presented as percentages. Culture and sensitivity testing was the predominant method; Escherichia spp., Enterococcus spp., and Staphylococcus aureus were the most targeted isolates. Variable incidences of resistance were detected against all major antimicrobial classes including reserved drugs such as
ceftazidime, piperacillin, gentamicin, ciprofloxacin, and chloramphenicol; the median frequency and range in resistant Gram-negative isolates were: 11 (0–100), 62 (0–100), 8 (0–100), 14 (0–93) and 12 (0–62) respectively. Ciprofloxacin was the most tested drug with the highest incidences of resistance in livestock slaughterhouses in Iran (93%), Nigeria (50%) and China (20%), and poultry slaughterhouses in Germany (21–81%) and Spain (56%). Spatial global distribution patterns for antimicrobial resistance were associated with previously reported magnitude of antibiotic use in livestock or poultry farming and, the implicit existence of jurisdictional policies to regulate antibiotic use. These data indicate that anthropogenic activities in farming systems are a major contributor to the cause and dissemination of antimicrobial resistance into the environment via slaughterhouse effluents