As características de hospitalidade e comensalidade no polo de turismo cervejeiro de Pinheiros e Vila Madalena (SP)

No presente artigo, buscou-se identificar e analisar características de hospitalidade e comensalidade presentes no polo cervejeiro de Pinheiros e Vila Madalena, localizados na cidade de São Paulo (SP), da perspectiva de gestores de estabelecimentos. Caracterizado pela alta concentração de bares e restaurantes especializados em cerveja artesanal, o polo estudado é resultado do avanço da cerveja artesanal no país e no mundo, tendência observada desde 2000. As singularidades da comensalidade, como dimensão de estudo da hospitalidade, permeiam a fruição e divisão igualitária da cerveja artesanal, inferindo particularidades identitárias próprias nos estabelecimentos cervejeiros. Estes se tornam mais que simples ambientes comerciais, desenvolvendo simbiose com o meio no qual estão inseridos – a anti-gentrificação da cerveja artesanal. A associação destes estabelecimentos com os atributos únicos dos bairros estudados conduziu à criação, ainda que involuntária, de um polo de turismo cervejeiro na cidade de São Paulo. Metodologicamente, este estudo caracteriza-se como qualitativo, exploratório e descritivo, e utilizou pesquisa de campo para a coleta de dados, através de entrevistas não estruturadas e observação participante. O referencial teórico foi elaborado a partir de pesquisa bibliográfica e documental. Observou-se a existência e aplicação dos conceitos de hospitalidade e comensalidade como diferenciais estratégicos de condução de negócios cervejeiros da região, como resultados empíricos. Como achados teóricos, os modelos analisados foram comprovados através de evidências, quando aplicadas ao circuito cervejeiro local

On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice

Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.Fil: Wada, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Hatakeyama, Yuto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Nakashima, Taiken. Hokkaido University; JapónFil: Nonami, Hiroshi. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Hakata, Makoto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Nakata, Keisuke. Ehime University; JapónFil: Hiraoka, Kenzo. University Of Yamanashi; JapónFil: Onda, Yayoi. Ehime University; JapónFil: Nakano, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japó

A novel in-frame deletion in the leucine zipper domain of C/EBPε leads to neutrophil-specific granule deficiency

Neutrophil-specific granule deficiency (SGD) is a rare autosomal recessive primary immunodeficiency characterized by neutrophil dysfunction, bilobed neutrophil nuclei and lack of neutrophil-specific granules. Defects in a myeloid-specific transcription factor, CCAAT/enhancer binding protein-ε (C/EBPε), have been identified in two cases in which homozygous frameshift mutations led to loss of the leucine zipper domain. In this study, we report a 55-y-old woman affected with SGD caused by a novel homozygous 2-aa deletion (ΔRS) in the leucine zipper domain of the C/EBPε gene. The patient showed characteristic neutrophil abnormalities and recurrent skin infections; however, there was no history of deep organ infections. Biochemical analysis revealed that, in contrast to the two frameshift mutations, the ΔRS mutant maintained normal cellular localization, DNA-binding activity, and dimerization, and all three mutants exhibited marked reduction in transcriptional activity. The ΔRS mutant was defective in its association with Gata1 and PU.1, as well as aberrant cooperative transcriptional activation of eosinophil major basic protein. Thus, the ΔRS likely impairs protein-protein interaction with other transcription factors, resulting in a loss of transcriptional activation. These results further support the importance of the leucine zipper domain of C/EBPε for its essential function, and indicate that multiple molecular mechanisms lead to SGD. © 2015 by The American Association of Immunologists, Inc

The ASTRO-H X-ray Observatory

The joint JAXA/NASA ASTRO-H mission is the sixth in a series of highly successful X-ray missions initiated by the Institute of Space and Astronautical Science (ISAS). ASTRO-H will investigate the physics of the high-energy universe via a suite of four instruments, covering a very wide energy range, from 0.3 keV to 600 keV. These instruments include a high-resolution, high-throughput spectrometer sensitive over 0.3-2 keV with high spectral resolution of Delta E < 7 eV, enabled by a micro-calorimeter array located in the focal plane of thin-foil X-ray optics; hard X-ray imaging spectrometers covering 5-80 keV, located in the focal plane of multilayer-coated, focusing hard X-ray mirrors; a wide-field imaging spectrometer sensitive over 0.4-12 keV, with an X-ray CCD camera in the focal plane of a soft X-ray telescope; and a non-focusing Compton-camera type soft gamma-ray detector, sensitive in the 40-600 keV band. The simultaneous broad bandpass, coupled with high spectral resolution, will enable the pursuit of a wide variety of important science themes.Comment: 22 pages, 17 figures, Proceedings of the SPIE Astronomical Instrumentation "Space Telescopes and Instrumentation 2012: Ultraviolet to Gamma Ray

Replication of Epstein-Barr Virus Primary Infection in Human Tonsil Tissue Explants

Epstein-Barr virus (EBV) may cause a variety of virus-associated diseases, but no antiviral agents have yet been developed against this virus. Animal models are thus indispensable for the pathological analysis of EBV-related infections and the elucidation of therapeutic methods. To establish a model system for the study of EBV infection, we tested the ability of B95–8 virus and recombinant EBV expressing enhanced green fluorescent protein (EGFP) to replicate in human lymphoid tissue. Human tonsil tissues that had been surgically removed during routine tonsillectomy were sectioned into small blocks and placed on top of collagen sponge gels in culture medium at the air-interface, then a cell-free viral suspension was directly applied to the top of each tissue block. Increasing levels of EBV DNA in culture medium were observed after 12–15 days through 24 days post-infection in tissue models infected with B95–8 and EGFP-EBV. Expression levels of eight EBV-associated genes in cells collected from culture medium were increased during culture. EBV-encoded small RNA-positive cells were detected in the interfollicular areas in paraffin-embedded sections. Flow cytometric analyses revealed that most EGFP+ cells were CD3− CD56− CD19+ HLA-DR+, and represented both naïve (immunoglobulin D+) and memory (CD27+) B cells. Moreover, EBV replication in this model was suppressed by acyclovir treatment in a dose-dependent manner. These data suggest that this model has potential for use in the pathological analysis of local tissues at the time of primary infection, as well as for screening novel antiviral agents

Effect of shelter acclimation on the post-release movement and putative predation mortality of hatchery-reared black-spot tuskfish Choerodon schoenleinii, determined by acoustic telemetry

In this study, the effect of shelter acclimation on the post-release movement and putative predation mortality of hatchery-reared black-spot tuskfish Choerodon schoenleinii was examined using acoustic telemetry. We acclimated four 1-year-old fish to shelters in cages before release and compared their movements with six nonacclimated fish. Since it was not possible to compare the behavioral pattern between the former and the latter fish due to the short periods the latter fish were available to be monitored, we also compared their movements with those of large nonacclimated fish that were less likely to be preyed upon. Sixty-seven percent of the nonacclimated fish showed atypical movements before the signals ceased to be detected, a pattern that suggested a predation event had occurred, whereas none of the acclimated and large nonacclimated fish showed the atypical movements. In addition, the probability of detection cessation was about 13 times lower in the acclimated than nonacclimated fish. The signal detection patterns suggest that the acclimated fish utilized night-time shelters from the first night after release, while the large nonacclimated fish started to utilize shelters several days after release. Therefore, it is likely that the shelter acclimation enhanced the shelter utilization by tuskfish, possibly decreasing post-release predation mortality

フジサンロク シンリン タイキ ビリョウ キタイ ケイソク システム オ モチイタ シンリン ト シガイチ ニオケル チッソ サンカブツ オゾン ノウド ヘンカ ノ ヒカク

We have developed a monitoring system for nitrogen oxide (NO), nitrogen dioxides (NO_2), and ozone (O_3) in theatmosphere in a Fujiyoshida forest site at the foot of Mt. Fuji. The air was sampled above the canopy at the height of 27 musing a flux tower in the observation site. The concentration of NO, NO_2 and O_3 were successfully observed continuously for amonth in September 2011. We tested the monitoring system using NO, NO_2 and O_3 analyzers with the length of 40 m PTFE tube in the laboratory inUenohara Campus of Teikyo University of Science. The observed data were in good agreements with the data obtained byAtmospheric Environmental Regional Observation System (AEROS) in the Uenohara city centre which located in a few kmaway from the Uenohara Campus. We constructed the monitoring system of NO, NO_2 and O_3 concentrations in the atmosphereabove the canopy in the Fujiyoshida forest site at the foot of Mt. Fuji. The primary data were successfully observed for amonth and discussed with the data obtained by AEROS in the Fujiyoshida city center. The concentrations of NO and NO_2 in the Fujiyoshida forest were increased in the early morning and in the evening, andwere smaller than in the city center. This suggested NO and NO_2 were emitted by traffics in the city center and diffused tothe forest. However the concentrations of O_3 in the forest were larger than in the Fujiyoshida city centre, especially in thenighttime. A possible interpretation is that O_3 in the city centre reacts with NO, and O_3 reduces its concentration. We willcontinue the observation and the observation results will give us more information of O_3 in the forest

Anaplastic hemangiopericytoma manifesting as a rapidly enlarging extracranial mass lesion

We, herein, present a patient with a recurrent anaplastic hemanigiopericytoma manifesting as a rapidly enlarging extracranial mass lesion, which was revealed by pathological and intraoperative findings. In practice, this case highlights the mandatory need for a careful long-term follow-up for patients with hemangiopericytoma, since recurrence with a greater degree of malignancy can develop following an extended disease-free interval, as such knowledge will be helpful for planning the optimal surgical procedures