Characterization of structures in biofilms formed by a Pseudomonas fluorescens isolated from soil

<p>Abstract</p> <p>Background</p> <p>Microbial biofilms represent an incompletely understood, but fundamental mode of bacterial growth. These sessile communities typically consist of stratified, morphologically-distinct layers of extracellular material, where numerous metabolic processes occur simultaneously in close proximity. Limited reports on environmental isolates have revealed highly ordered, three-dimensional organization of the extracellular matrix, which may hold important implications for biofilm physiology <it>in vivo</it>.</p> <p>Results</p> <p>A <it>Pseudomonas </it>spp. isolated from a natural soil environment produced flocculent, nonmucoidal biofilms <it>in vitro </it>with unique structural features. These mature biofilms were made up of numerous viable bacteria, even after extended culture, and contained up to 50% of proteins and accumulated 3% (by dry weight) calcium, suggesting an important role for the divalent metal in biofilm formation. Ultrastructurally, the mature biofilms contained structural motifs consisting of dense, fibrillary clusters, nanofibers, and ordered, honeycomb-like chambers enveloped in thin sheets.</p> <p>Conclusion</p> <p>Mature biofilms contained living bacteria and were structurally, chemically, and physiologically heterogeneous. The principal architectural elements observed by electron microscopy may represent useful morphological clues for identifying bacterial biofilms <it>in vivo</it>. The complexity and reproducibility of the structural motifs observed in bacterial biofilms appear to be the result of organized assembly, suggesting that this environmental isolate may possess ecological advantages in its natural habitat.</p

Crossover between Thermally Assisted and Pure Quantum Tunneling in Molecular Magnet Mn12-Acetate

The crossover between thermally assisted and pure quantum tunneling has been studied in single crystals of high spin (S=10) uniaxial molecular magnet Mn12 using micro-Hall-effect magnetometry. Magnetic hysteresis and relaxation experiments have been used to investigate the energy levels that determine the magnetization reversal as a function of magnetic field and temperature. These experiments demonstrate that the crossover occurs in a narrow (0.1 K) or broad (1 K) temperature interval depending on the magnitude of the field transverse to the anisotropy axis.Comment: 5 pages, 4 figure

Systolic and Hyper-Systolic Algorithms for the Gravitational N-Body Problem, with an Application to Brownian Motion

A systolic algorithm rhythmically computes and passes data through a network of processors. We investigate the performance of systolic algorithms for implementing the gravitational N-body problem on distributed-memory computers. Systolic algorithms minimize memory requirements by distributing the particles between processors. We show that the performance of systolic routines can be greatly enhanced by the use of non-blocking communication, which allows particle coordinates to be communicated at the same time that force calculations are being carried out. Hyper-systolic algorithms reduce the communication complexity at the expense of increased memory demands. As an example of an application requiring large N, we use the systolic algorithm to carry out direct-summation simulations using 10^6 particles of the Brownian motion of the supermassive black hole at the center of the Milky Way galaxy. We predict a 3D random velocity of 0.4 km/s for the black hole.Comment: 33 pages, 10 postscript figure

Subsidizing Religious Participation through Groups: A Model of the “Megachurch” Strategy for Growth

Either despite or because of their non-traditional approach, megachurches have grown significantly in the United States since 1980. This paper models religious participation as an imperfect public good which, absent intervention, yields suboptimal participation by members from the church’s perspective. Megachurches address this problem in part by employing secular-based group activities to subsidize religious participation that then translates into an increase in the attendees’ religious investment. This strategy not only allows megachurches to attract and retain new members when many traditional churches are losing members but also results in higher levels of an individual’s religious capital. As a result, the megachurch may raise expectations of members’ levels of commitment and faith practices. Data from the FACT2000 survey provide evidence that megachurches employ groups more extensively than other churches, and this approach is consistent with a strategy to use groups to help subsidize individuals’ religious investment. Religious capital rises among members of megachurches relative to members of non-megachurches as a result of this strategy

The nature of late-type spiral galaxies: structural parameters, optical and near-infrared colour profiles, and dust extinction

We analyse V and H-band surface photometry of a sample of 18 Sb-Sd galaxies. Combining high resolution HST images with ground-based NIR observations, we extract photometric profiles, which cover the whole disk and provide the highest possible resolution. This is the first photometric study of late-type spirals for which the stellar kinematics have been measured. For 10 out of the 18 galaxies, HST data in both F160W (H) and F606W (V) are available, and, for those, we present colour maps and radial colour profiles at the resolution of the Hubble Space Telescope. Colours vary significantly from galaxy to galaxy, but tend to be highly homogeneous within each galaxy, with smooth and flat colour profiles. Some of the colour maps show jumps in the inner regions, likely due to dust. We determine extinction-maps in an almost model-independent way using the V-H colour map and the SAURON Mg b absorption line map of Ganda et al. (2007). The maps show that A_V ranges from 0 to 2 mag, in the center from 0 to 1.5 mag, in agreement with the models of Tuffs et al. (2004). We describe the surface brightness profiles as the superposition of an exponential disk and a Sersic bulge. The bulges are small (0.1-2.5 kpc), and show a shape parameter n ranging from ~ 0.7 to 3, with a mean value smaller than two: well below the value for the 'classical' de Vaucouleurs bulges. Most galaxies (16 out of 18) show a central light excess above the Sersic fit to the bulge, which can be interpreted as a nuclear cluster, as shown by previous studies. We provide zero-order estimates for the magnitude of these components. We discuss the correlations among the structural galaxy parameters and with other relevant quantities (abridged).Comment: 28 pages, 12 figures and 7 tables. Accepted for publication in MNRAS. Higher resolution version available at http://www.astro.rug.nl/~peletier/ganda2009.pd

A Randomized Trial of Manual Phone Calls Versus Automated Text Messages for Peripheral Nerve Block Follow-Ups.

Mobile phone applications (apps) have been used for patient follow-up in the postoperative period, specifically to assess for complications and patient satisfaction. Few studies have evaluated their use in regional anesthesia. The objective of this study was to compare follow-up response rates using manual phone calls versus an automated patient outreach (APO) app for peripheral nerve block patients. We hypothesized that the response rate would be higher in the APO group. A mobile app, JeffAnesthesia, was developed, which sends notifications to patients to answer survey questions in the app. We randomly assigned patients who received peripheral nerve blocks for postoperative pain to either a manual phone call or an APO app group, with follow-up in each category occurring between postoperative days (POD) 14-21 and 90-100. In total, 60 patients were assigned to the phone call group and 60 patients to the APO app group. Between POD 14-21, 9 (15%) patients were reached in the manual phone call arm, and 16 (26.7%) patients were reached in the APO arm (p = 0.117). At POD 90-100, follow-up was successful with 5 (8.2%) in the manual phone call group vs. 3 (5.0%) patients in the APO app group (p = 0.300). Overall response rate was poor, with comparable response rates between groups. The APO method may reduce time spent by anesthesia staff on follow-up calls, but our data do not suggest this method improves response rates significantly. Further studies are needed to better understand the reasons for the poor response rate and strategies for improvement

The Third Gravitational Lensing Accuracy Testing (GREAT3) Challenge Handbook

The GRavitational lEnsing Accuracy Testing 3 (GREAT3) challenge is the third in a series of image analysis challenges, with a goal of testing and facilitating the development of methods for analyzing astronomical images that will be used to measure weak gravitational lensing. This measurement requires extremely precise estimation of very small galaxy shape distortions, in the presence of far larger intrinsic galaxy shapes and distortions due to the blurring kernel caused by the atmosphere, telescope optics, and instrumental effects. The GREAT3 challenge is posed to the astronomy, machine learning, and statistics communities, and includes tests of three specific effects that are of immediate relevance to upcoming weak lensing surveys, two of which have never been tested in a community challenge before. These effects include realistically complex galaxy models based on high-resolution imaging from space; spatially varying, physically-motivated blurring kernel; and combination of multiple different exposures. To facilitate entry by people new to the field, and for use as a diagnostic tool, the simulation software for the challenge is publicly available, though the exact parameters used for the challenge are blinded. Sample scripts to analyze the challenge data using existing methods will also be provided. See http://great3challenge.info and http://great3.projects.phys.ucl.ac.uk/leaderboard/ for more information.Comment: 30 pages, 13 figures, submitted for publication, with minor edits (v2) to address comments from the anonymous referee. Simulated data are available for download and participants can find more information at http://great3.projects.phys.ucl.ac.uk/leaderboard