Single and mixed lactic acid bacteria culture fermentation in red bean milk for development of a functional beverage

This study was to evaluate the effect of fermentation on the total phenolic contents (TPCs) and antioxidant activities (AA) in red bean milk (RBM) using three different lactic acid bacteria (LAB); Streptococcus thermophilus TISTR 894 (ST), Lactobacillus plantarum 299V (LP), and Lactobacillus casei 388 (LC), as a single (SCF) or a mixed culture fermentation (MCF). The fermentation increased TPCs value and AA values in the RBM, irrespective of fermentation technique or LAB cultures. Among SCF, the SCF-LP showed the highest values of TPCs, DPPH, and FRAP while SCF-LC had the highest ORAC assay values. The MCF could lead to higher TPCs and AA than those of SCF. Especially, MCF using three cultures of LAB presented the highest TPCs, DPPH, and FRAP assay (1.47 mg GAE/g DW, 11.59 µmole TE/g DW, and 4.89 µmole TE/g DW, respectively). Moreover, the study showed that the MCF can effectively improve the TPCs and AA, thereby indicating additional health benefits of fermentation

Interactions between Saccharomyces cerevisiae and Hanseniaspora guilliermondii: cell-cell contact mechanism

Several studies have shown that the early death of non-Saccharomyces during wine fermentations are due to yeast-yeast interactions induced by Saccharomyces cerevisiae (Sc) through different mechanisms such as growth arrest mediated by a cell-cell contact mechanism (Nissen et al. 2003) and death mediated by killer-like toxins (Pérez-Nevado et al 2006; Albergaria et al. 2010). Besides, previous work also showed that death of non-Saccharomyces in co-cultivation with Sc is always triggered at the end of exponential growth phase (Pérez-Nevado et al 2006). In order to investigate the role of cell-cell contact in the early death of non-Saccharomyces, we performed assays in which Sc cells pre-grown at enological growth conditions for 12 and 48 h, respectively, were in direct contact with Hanseniaspora guilliermondii (Hg) cells at high cellular density (107-108 cells/ml) in a carbon-free medium. As a negative control we performed similar assays in which Sc and Hg cells were separated by a dialysis tube (pore cut-off of 1000 kDa) and as a positive control a single Hg culture. Results showed that Hg cell density decreased by 2 orders of magnitude (i.e. from 108-106 cells/ml) in contact with 48 h-grown Sc cells, while its viability remained unchanged (108cfu/ml) in the presence of 12 h-grown Sc cells. Moreover, Hg viability was not affected both in the dialysis tube experiments and single culture, which confirmed the death-induced cell-cell contact phenomenon

Incidents and potential adverse health effects of serious food fraud cases originated in Asia

Food fraud has long been regarded as a major issue within the food industry and is associated with serious economic and public health concerns. Economically motivated adulteration, the most common form of food fraud, has consequences for human health, ranging from mild to life-threatening conditions. Despite the potential harm and public health threats posed by food fraud, limited information on incidents causing illness has been reported. Enhancing the food control system on the Asian continent has become crucial for global health and trade considerations. Food fraud databases serve as valuable tools, assisting both the food industry and regulatory bodies in mitigating the vulnerabilities associated with fraudulent practices. However, the availability of accessible food fraud databases for Asian countries has been restricted. This review highlights detrimental food fraud cases originating in Asian countries, including sibutramine in dietary supplements, plasticizer contamination, gutter oil, and the adulteration of milk. This comprehensive analysis encompasses various facets, such as incident occurrences, adverse health effects, regulatory frameworks, and mitigation strategies

Yeast Interactions in Inoculated Wine Fermentation

The use of selected starter culture is widely diffused in winemaking. In pure fermentation, the ability of inoculated Saccharomyces cerevisiae to suppress the wild microflora is one of the most important feature determining the starter ability to dominate the process. Since the wine is the result of the interaction of several yeast species and strains, many studies are available on the effect of mixed cultures on the final wine quality. In mixed fermentation the interactions between the different yeasts composing the starter culture can led the stability of the final product and the analytical and aromatic profile. In the present review, we will discuss the recent developments regarding yeast interactions in pure and in mixed fermentation, focusing on the influence of interactions on growth and dominance in the process

Non-conventional yeast species for lowering ethanol content of wines

Rising sugar content in grape must, and the concomitant increase in alcohol levels in wine, are some of the main challenges affecting the winemaking industry nowadays. Among the several alternative solutions currently under study, the use of nonconventional yeasts during fermentation holds good promise for contributing to relieve this problem. Non-Saccharomyces wine yeast species comprise a high number or species, so encompassing a wider physiological diversity than Saccharomyces cerevisiae. Indeed, the current oenological interest of these microorganisms was initially triggered by their potential positive contribution to the sensorial complexity of quality wines, through the production of aroma and other sensory-active compounds. This diversity also involves ethanol yield on sugar, one of the most invariant metabolic traits of S. cerevisiae. This review gathers recent research on non-Saccharomyces yeasts, aiming to produce wines with lower alcohol content than those from pure Saccharomyces starters. Critical aspects discussed include the selection of suitable yeast strains (considering there is a noticeable intra-species diversity for ethanol yield, as shown for other fermentation traits), identification of key environmental parameters influencing ethanol yields (including the use of controlled oxygenation conditions), and managing mixed fermentations, by either the sequential or simultaneous inoculation of S. cerevisiae and non-Saccharomyces starter cultures. The feasibility, at the industrial level, of using non-Saccharomyces yeasts for reducing alcohol levels in wine will require an improved understanding of the metabolism of these alternative yeast species, as well as of the interactions between different yeast starters during the fermentation of grape must.Experimental work by researchers from ICVV is supported by the Spanish Government trough MINECO and FEDER funds: MINECO AGL2012-32064 and AGL2015-63629-R grants, INIA RM2012-00007-00-00 grant, MINECO RTC-2014-2186-2 grant, MINECO training contract for AR, MINECO Formación Postdoctoral contract for JC. Experimental work by the authors from Polytechnic University of Marche is supported by Ricerca Scientifica di Ateneo RSA2015.Peer Reviewe

Road to The Red Carpet of Edible Crickets through Integration into the Human Food Chain with Biofunctions and Sustainability: A Review

The Food and Agriculture Organization of the United Nations (FAO) estimates that more than 500 million people, especially in Asia and Africa, are suffering from malnutrition. Recently, livestock farming has increased to supply high-quality protein, with consequent impact on the global environment. Alternative food sources with high nutritive values that can substitute livestock demands are urgently required. Recently, edible crickets have been promoted by the FAO to ameliorate the food crisis. In this review, the distribution, nutritive values, health-promoting properties (antioxidant, anti-inflammatory, anti-diabetic and anti-obesity), safety, allergenicity as well as the potential hazards and risks for human consumption are summarized. Cricket farming may help to realize the United Nations sustainable development goal No. 2 Zero Hunger. The sustainability of cricket farming is also discussed in comparison with other livestock. The findings imply that edible crickets are safe for daily intake as a healthy alternative diet due to their high protein content and health-promoting properties. Appropriate use of edible crickets in the food and nutraceutical industries represents a global business potential. However, people who are allergic to shellfish should pay attention on cricket allergy. Thus, the objective of this review was to present in-depth and up-to-date information on edible crickets to advocate and enhance public perception of cricket-based food

Storage Stability of High Fiber Snack Bar

Background: Recently, there has been considerable interest in increasing the dietary fiber content in food products because of inadequate dietary fiber consumption when considering the daily recommended intake. To increase dietary fiber intake, dietary fiber fortified foods are recommended. This study aimed to develop a high fiber snack bar (HFSB) using a combination of Jerusalem artichoke powder (JAP) and low-fat desiccated coconut (LFDC) as sources of dietary fiber. Methods: The changes in physicochemical and microbiological properties, and sensory acceptability were measured during storage at 35 oC and 45 oC for 12 weeks. Therefore, the shelf-life of the products was calculated by Q10 test. Results: The HFSB had a higher L* value (lightness) than control (C) due to the addition of LFDC. Total dietary fiber of the HFSB was approximately 3.7 times higher than that of the C formula. The L*, a*, and b* values of both C and HFSB were statistically significant different (p<0.05) after storage. The total color different (E) values of the HFSB were higher than those of the C formula due to inulin from JAP, which participated in the Maillard reaction. During storage, the moisture content (MC) and water activity (aw) of the HFSB remained more stable compared to those of the C due to the water-holding capacity of the fiber used. The aw of the C and HFSB during storage were in the range of 0.57 to 0.60 and 0.53 to 0.57, respectively. Those ranges should be stable against microbial growth. Higher storage temperature would increase the TBARS values and decrease the pH (p<0.05) of the C and HFSB due to deterioration. In terms of shelf-life calculation, the C and HFSB snack bar could be kept in metalized polyester at 30 oC for 11 weeks. Conclusion: The JAP and LFDC exhibited great potential for use as fiber ingredients. Although the JAP and LFDC influenced the physicochemical properties and sensory acceptability, the shelf-life of both C and HFSB was comparable. Therefore, further studies should be conducted to extend the shelf-life of the formulated snack bar. Keywords: Snack bar, High fiber, Jerusalem artichoke, Low-fat desiccated coconut, Shelf-life Received: 8 May 2019 Reviewed: 14 June 2019 Revised: 26 June 2019 Accepted: 19 July 2019 DOI: 10.35898/ghmj-3358

The Effects of Different Roasting Methods on the Phenolic Contents, Antioxidant Potential, and In Vitro Inhibitory Activities of Sacha Inchi Seeds

Roasted sacha inchi seeds are now commercialized as a health food product, but the influence of roasting methods on their proclaimed health effects has yet to be explored. This study investigated the total phenolic contents (TPCs), antioxidant potential, and inhibitory activities of raw and roasted sacha inchi seeds in vitro. Individual phenolics in raw seeds were also identified in an attempt to explain the bioactivities of the seeds. The results suggested that roasting in a cooking pan, vacuum oven, and tray dryer had distinct impact on TPC in sacha inchi seeds, and thus differentially altered their antioxidant and inhibitory properties. Seeds that underwent roasting exhibited 1.5–2.7-fold higher antioxidant potentials than raw seeds. Certain roasting methods provided the products with anti-α-amylase and anti-cholinesterase activities, while inhibitions of these enzymes were not detected in raw seeds. Roasted seeds also possessed superior anti-lipase and anti-glycation activities when compared with raw seeds (up to 1.7- and 4.8-fold, respectively). The inhibitory properties observed in the seed samples might be attributed to their p-coumaric acid, ferulic acid, and quercetin, as these potential enzyme inhibitors were predominant in raw seeds. The overall results showed that pan-roasting could be used to obtain relatively high health benefits from the antioxidant and inhibitory activities of sacha inchi seeds. The information obtained from this study may serve as the basis for the proper processing of sacha inchi seeds to optimize their functional food and nutraceutical applications