Transformação de milho atraves de bombeamento com microparticulas : otimização de parametros fisicos e biologicos

Orientador: Paulo ArrudaDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de BiologiaMestradoGenetica de PlantasMestre em Ciências Biológica

Desenvolvimento De Um Cassete Para A Expressão De Proteìnas Heterólogas Especificamente Em Sementes De Plantas Transgênicas

Desenvolvimento de um cassete para a expressão de proteínas heterólogas especificamente em sementes de plantas transgênicas, que no seu desenvolvimento utilizou as regiões: 1) região reguladora (promotor) do gene que codifica a γ-kafirina de sorgo, 2) região correspondente ao peptídeo sinal de uma a-prolamina de Coix, e 3) a região contendo o sinal de poliadenilação do 35S CaMV, sendo que, para exemplificar a aplicação do cassete de expressão foi construído o vetor binário pB-PgK-PShGH que apresenta inserção de um fragmento de DNA codificador do hormônio de crescimento humano (hGH).BR9805166 (A); BR9805166 (C1)A01H5/10A61K38/56A61K38/58A61P43/00C07K14/415C12N15/18C12N15/29C12N15/82C12N15/83C07K14/415A61K35/78C12N15/18C12N15/29C12N15/82C12N15/83BR19989805166A01H5/10A61K38/56A61K38/58A61P43/00C07K14/415C12N15/18C12N15/29C12N15/82C12N15/83C07K14/415A61K35/78C12N15/18C12N15/29C12N15/82C12N15/8

Evaluation de divergence de monocot et d'eudicot en utilisant le transcriptome de canne à sucre

Corresponding author : e-mail [email protected];International audienceOver 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA sequences from other angiosperms, including the genomes of Arabidopsis and rice (Oryza sativa). Approximately two-thirds of the sugarcane transcriptome have similar sequences in Arabidopsis. These sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angiosperm functions. The remaining sequences represent putative monocot-specific genetic material, one-half of which were found only in sugarcane. These monocot-specific cDNAs represent either novelties or, in many cases, fast-evolving sequences that diverged substantially from their eudicot homologs. The wide comparative genome analysis presented here provides information on the evolutionary changes that underlie the divergence of monocots and eudicots. Our comparative analysis also led to the identification of several not yet annotated putative genes and possible gene loss events in Arabidopsi

Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Crop Sugarcane

To contribute to our understanding of the genome complexity of sugarcane, we undertook a large-scale expressed sequence tag (EST) program. More than 260,000 cDNA clones were partially sequenced from 26 standard cDNA libraries generated from different sugarcane tissues. After the processing of the sequences, 237,954 high-quality ESTs were identified. These ESTs were assembled into 43,141 putative transcripts. Of the assembled sequences, 35.6% presented no matches with existing sequences in public databases. A global analysis of the whole SUCEST data set indicated that 14,409 assembled sequences (33% of the total) contained at least one cDNA clone with a full-length insert. Annotation of the 43,141 assembled sequences associated almost 50% of the putative identified sugarcane genes with protein metabolism, cellular communication/signal transduction, bioenergetics, and stress responses. Inspection of the translated assembled sequences for conserved protein domains revealed 40,821 amino acid sequences with 1415 Pfam domains. Reassembling the consensus sequences of the 43,141 transcripts revealed a 22% redundancy in the first assembling. This indicated that possibly 33,620 unique genes had been identified and indicated that >90% of the sugarcane expressed genes were tagged