Effects of Systemic Erythropoietin on Ischemic Wound Healing in Rats

Results of in vivo studies have shown erythropoietin (EPO) is associated with anti-inflammatory, anti-apoptotic, and cell protective effects on wound healing. These effects are dose-dependent. The aim of this study was to evaluate whether the duration of EPO treatment affects the healing process in the ischemic wound. Forty-two (42) Sprague-Dawley rats were anesthetized, wounded with H-shaped flaps, and randomized to 2 groups; Group 1 received 400 u/kg/day EPO and Group 2 received a saline solution, both via intraperitoneal injection following the wounding. All substances were administered once daily at the same time for up to 10 days after surgery. At days 3, 5, and 10, 7 rats from each group were sacrificed. Skin samples were stained with hematoxylin/eosin, viewed under an optical microscope at 10X and 40X magnification, and analyzed by blinded investigators for re-epithelialization, neovascularization amount and maturation of granulation tissue, inflammatory cells, and ulcer healing using an evaluation scale where 0 = none; 1 = partial; 2 = complete, but immature/thin: and 4 = complete and mature. Blood hemoglobin and hematocrit levels also were measured. Data were analyzed using ANOVA one-way test (P < 0.05). Hemoglobin and hematocrit levels rose while subsequent doses of EPO were administered over time, accompanied by a transient surge in healing on day 5, when differences in healing scores were significant. Flap necrosis, ulceration, and abscess were noted on post-wounding day 10 near the pedicle. The study showed EPO therapy can improve wound healing early in the post-wounding period but can reduce wound healing after post-injury treatment day 5. Further research is necessary, particularly to establish how EPO influences the microcirculation and rheology

The Left-wing Extremism in the Czech Republic

Téma této bakalářské práce je: "Levicový extremismus v České republice". "Je společenská nebezpečnost u levicového extremismu srovnatelná se společenskou nebezpečností u pravicového extremismu ?". I tato složitá otázka a možná odpověď je její součástí. Hlavním cílem této práce je zabývat se extremismem, jako jedním ze sociálně patologických jevů. Protože je to téma obsahově náročné, zaměřuje se jen na levicový extremismus. V první části je představen pojem extremismus a jeho rozdělení. Poté se věnuje problémům levicového extremismu z pohledu jeho sociologických a právních aspektů ve vztahu k jednotlivcům, hnutím a organizacím, které lze zařadit do tohoto extremistického spektra. Jsou zde uvedeny konkrétní organizace a jejich současná činnost s celorepublikovou působností. Dalším fenoménem, který se projevuje i na této straně extremistického spektra je divácké násilí. Zde je záměrem upozornit na některé současné projevy s ideologickým podtextem, zejména na našich fotbalových stadionech. V souvislosti nejen s anarchoautonomním hnutím jsou zde také zmíněna některá ze subkulturních hnutí. Powered by TCPDF (www.tcpdf.org)This is the subject of the bachelor-work: "The left-wing extremism in the Czech Republic". Is the danger for society from the left-wing extremism comparison with the right-wing extremism danger? This question is complicated, but there is the answer as a part of it in the work too. The principal aim of this work is the extremism, one of the social pathologic phenomenous. Because it is the subject including a very comprehensive matter, the authorś efforts are in the focus to the one only his part, to the left-wing extremism. First of all the definition of extremism and his different parts are presented. Later appear problems of left-wing extremism from the point of view his sociological and juridical aspects in the relation to the singles, movements and organisations, the may be closed in this extreme spectrum. They are named concrete organisations and their contemporary aktivities operating througout the republic territory. The next phenomenon manifesting on this part of extreme spectrum are onlooker violations. Here is an intention to draw attention to the actuell manifestations with an ideological overton, especially on our football stadiums. Not only in the continuity with the anarchist-autonomous movement there are mentioned any of the sub-cultural movements (PUNK, DIY, Free Techno Music ) too. Powered...JabokJabokProtestant Theological FacultyEvangelická teologická fakult

Sequential Measurements of Pentraxin 3 Serum Levels in Patients with Ventilator-Associated Pneumonia: A Nested Case-Control Study

Purpose. The main purpose of this study was to investigate the dynamics of pentraxin 3 (PTX3) compared with procalcitonin (PCT) and C-reactive protein (CRP) in patients with suspicion of ventilator-associated pneumonia (VAP). Materials and Methods. We designed a nested case-control study. This study was performed in the Surgical Intensive Care Unit of a tertiary care academic university and teaching hospital. Ninety-one adults who were mechanically ventilated for >48 hours were enrolled in the study. VAP diagnosis was established among 28 patients following the 2005 ATS/IDSA guidelines. Results. The median PTX3 plasma level was 2.66 ng/mL in VAP adults compared to 0.25 ng/mL in non-VAP adults (p<0.05). Procalcitonin and CRP levels did not significantly differ. Pentraxin 3, with a 2.56 ng/mL breakpoint, had 85% sensitivity, 86% specificity, 75% positive predictive value, and 92.9% negative predictive value for VAP diagnosis (AUC = 0.78). Conclusions. With the suspicion of VAP, a pentraxin 3 plasma breakpoint of 2.56 ng/mL could contribute to the decision of whether to start antibiotics