Interprofessional Community Schools

This presentation details the key components of community schools and their importance to community members and students alike. Comprised of four primary pillars, these schools focus on expanding learning opportunities, bringing in a variety of student supports, serving families collectively, while emphasizing the collaborative element of leadership. Community schools serve as an integration point of various elements of a community to come together in order to produce maximum benefits for all.https://dune.une.edu/caiepspring2024/1010/thumbnail.jp

WEATHERING, RADIOGENIC ISOTOPES, AND MARINE RECORDS OF GLACIAL DYNAMICS

Glacial advance and retreat is related to numerous climate system feedbacks; yet, this dynamic glacial activity tends to erase its own terrestrial record. As a result, deep-sea sediments may be the best archives for studying past glacial processes. Interpretations of these archives depend on understanding terrestrial sources to the marine sediments. Systematic spatial variations in dissolved riverine and soil leachate Sr, Nd and Pb isotopes across an ~175 km transect from the Greenland Ice Sheet to the coast present an analog for temporal changes during glacial retreat. Specifically, the offset between dissolved (riverine or soil leachates) and bulk sediment (bedload or leached soil) isotopes is highest in young glacial sediments close to the ice sheet and approaches zero in 10 ky old glacial sediments at the coast. This difference is attributed to a transition from preferential chemical weathering of trace minerals and/or radiation damaged sites in freshly comminuted, ice-proximal sediments to predominant weathering of less radiogenic (Sr and Pb) and more radiogenic (Nd) isotopes from bulk major minerals in more extensively weathered coastal material. These isotopes are transported to the ocean where the residence time of Sr is too long to be an effective tracer of local or regional glacial processes; however, the short residence time of Pb makes it an excellent tracer of local chemical weathering processes and the intermediate residence time of Nd allows application to region studies. Data from IODP Sites 1302/3 (3550 m water depth) in the NW Atlantic illustrate that seawater Pb and Nd isotopes preserved in authigenic FeMn-oxide coatings respond dramatically to retreat of the Laurentide Ice Sheet during the penultimate glacial termination (T2; 135-129 ka) and to rapid variations during Dansgaard-Oeschger cycles. These data suggest deep-sea radiogenic isotopes preserve a more detailed record of the long term history of ice sheet dynamics than terrestrial proxies. The systematic variation in chemical weathering linked to ice sheet retreat and reflected in deep-sea isotope records may also help refine estimates of past and future carbon cycling and fluxes of nutrients and isotopes to the ocean associated with high latitude climate change

The global food environment transition based on the socio-demographic index

Food environments are a critical point for reorienting the food system towards sustainable diets, as they are directly where consumers make decisions about which foods to acquire. Using global data, we examined shifts in food environments, and the availability, affordability, convenience, and quality of foods within them, over time on the basis of socio-demographic index (SDI) country groupings. Our findings provide evidence of a food environment transition where built environments have shifted from traditional to modern retail outlets between 2005 and 2019 and the availability and affordability of nutrient-rich foods has increased alongside growing sales of ultra-processed and ready-to-eat foods. This transition has implications for the identification of policy and program levers for promoting healthy and sustainable diets and reducing malnutrition globally

Connectivity mapping (ssCMap) to predict A20-inducing drugs and their antiinflammatory action in cystic fibrosis

Cystic fibrosis (CF) lung disease is characterized by chronic and exaggerated inflammation in the airways. Despite recent developments to therapeutically overcome the underlying functional defect in the cystic fibrosis transmembrane conductance regulator, there is still an unmet need to also normalize the inflammatory response. The prolonged and heightened inflammatory response in CF is, in part, mediated by a lack of intrinsic down-regulation of the proinflammatory NF-κB pathway. We have previously identified reduced expression of the NF-κB down-regulator A20 in CF as a key target to normalize the inflammatory response. Here, we have used publicly available gene array expression data together with a statistically significant connections’ map (sscMap) to successfully predict drugs already licensed for the use in humans to induce A20 mRNA and protein expression and thereby reduce inflammation. The effect of the predicted drugs on A20 and NF-κB(p65) expression (mRNA) as well as proinflammatory cytokine release (IL-8) in the presence and absence of bacterial LPS was shown in bronchial epithelial cells lines (16HBE14o−, CFBE41o−) and in primary nasal epithelial cells from patients with CF (Phe508del homozygous) and non-CF controls. Additionally, the specificity of the drug action on A20 was confirmed using cell lines with tnfαip3 (A20) knockdown (siRNA). We also show that the A20-inducing effect of ikarugamycin and quercetin is lower in CF-derived airway epithelial cells than in non-CF cells

A Simultaneous Dual-site Technosignature Search Using International LOFAR Stations

The Search for Extraterrestrial Intelligence aims to find evidence of technosignatures, which can point toward the possible existence of technologically advanced extraterrestrial life. Radio signals similar to those engineered on Earth may be transmitted by other civilizations, motivating technosignature searches across the entire radio spectrum. In this endeavor, the low-frequency radio band has remained largely unexplored; with prior radio searches primarily above 1 GHz. In this survey at 110-190 MHz, observations of 1,631,198 targets from TESS and Gaia are reported. Observations took place simultaneously with two international stations (noninterferometric) of the Low Frequency Array in Ireland and Sweden. We can reject the presence of any Doppler drifting narrowband transmissions in the barycentric frame of reference, with equivalent isotropic radiated power of 10 17 W, for 0.4 million (or 1.3 million) stellar systems at 110 (or 190) MHz. This work demonstrates the effectiveness of using multisite simultaneous observations for rejecting anthropogenic signals in the search for technosignatures.Comment: 15 Pages, 16 Figures, 2 Machine Readable Table

Lack of Antinociceptive Cross-Tolerance With Co-Administration of Morphine and Fentanyl Into the Periaqueductal Gray of Male Sprague-Dawley Rats

Tolerance to the antinociceptive effect of mu-opioid receptor (MOPr) agonists, such as morphine and fentanyl, greatly limits their effectiveness for long-term use to treat pain. Clinical studies have shown that combination therapy and opioid rotation can be used to enhance opioid-induced antinociception once tolerance has developed. The mechanism and brain regions involved in these processes are unknown. The purpose of this study was to evaluate the contribution of the ventrolateral periaqueductal gray (vlPAG) to antinociceptive tolerance and cross-tolerance between administration and co- administration of morphine and fentanyl. Tolerance was induced by pretreating rats with morphine or fentanyl or low-dose combination of morphine and fentanyl into the vlPAG followed by assessment of cross-tolerance to the other opioid. In addition, tolerance to the combined treatment was assessed. Cross-tolerance did not develop between repeated vlPAG microinjections of morphine and fentanyl. Likewise, there was no evidence of cross-tolerance from morphine or fentanyl to co-administration of morphine and fentanyl. Co-administration did not cause cross-tolerance to fentanyl. Cross- tolerance was only evident to morphine or morphine and fentanyl combined in rats pretreated with co-administration of low-doses of morphine and fentanyl. In conclusion, cross-tolerance does not develop between morphine and fentanyl within the vlPAG. This finding is consistent with the functionally selective signaling that has been reported for antinociception and tolerance following morphine and fentanyl binding to the MOPr. This research supports the notion that combination therapy and opioid rotation may be useful clinical practices to reduce opioid tolerance and other side effects. Perspective: This preclinical study shows that there is a reduction in cross tolerance between morphine and fentanyl within the periaqueductal gray which is key brain region in opioid antinociception and tolerance

Anthrax Toxins Inhibit Neutrophil Signaling Pathways in Brain Endothelium and Contribute to the Pathogenesis of Meningitis

Anthrax meningitis is the main neurological complication of systemic infection with Bacillus anthracis approaching 100% mortality. The presence of bacilli in brain autopsies indicates that vegetative bacteria are able to breach the blood-brain barrier (BBB). The BBB represents not only a physical barrier but has been shown to play an active role in initiating a specific innate immune response that recruits neutrophils to the site of infection. Currently, the basic pathogenic mechanisms by which B. anthracis penetrates the BBB and causes anthrax meningitis are poorly understood.Using an in vitro BBB model, we show for the first time that B. anthracis efficiently invades human brain microvascular endothelial cells (hBMEC), the single cell layer that comprises the BBB. Furthermore, transcriptional profiling of hBMEC during infection with B. anthracis revealed downregulation of 270 (87%) genes, specifically key neutrophil chemoattractants IL-8, CXCL1 (Gro alpha) and CXCL2 (Gro beta), thereby strongly contrasting hBMEC responses observed with other meningeal pathogens. Further studies using specific anthrax toxin-mutants, quantitative RT-PCR, ELISA and in vivo assays indicated that anthrax toxins actively suppress chemokine production and neutrophil recruitment during infection, allowing unrestricted proliferation and dissemination of the bacteria. Finally, mice challenged with B. anthracis Sterne, but not the toxin-deficient strain, developed meningitis.These results suggest a significant role for anthrax toxins in thwarting the BBB innate defense response promoting penetration of bacteria into the central nervous system. Furthermore, establishment of a mouse model for anthrax meningitis will aid in our understanding of disease pathogenesis and development of more effective treatment strategies