Synthetic chemical inducers and genetic decoupling enable orthogonal control of the rhaBAD promoter

External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD promoter has properties superior to more commonly-used inducible expression systems, but is marred by transient expression caused by degradation of the native inducer, L-rhamnose. To address this problem, 35 analogs of L-rhamnose were screened for induction of the rhaBAD promoter, but no strong inducers were identified. In the native configuration, an inducer must bind and activate two transcriptional activators, RhaR and RhaS. Therefore, the expression system was reconfigured to decouple the rhaBAD promoter from the native rhaSR regulatory cascade so that candidate inducers need only activate the terminal transcription factor RhaS. Re-screening the 35 compounds using the modified rhaBAD expression system revealed several promising inducers. These were characterised further to determine the strength, kinetics and concentration-dependence of induction; whether the inducer was used as a carbon source by E. coli; and the modality (distribution) of induction among populations of cells. L-Mannose was found to be the most useful orthogonal inducer, providing an even greater range of induction than the native inducer Lrhamnose, and crucially, allowing sustained induction instead of transient induction. These findings address the key limitation of the rhaBAD expression system, and suggest it may now be the most suitable system for many applications

Coarse-grained entropy and causal holographic information in AdS/CFT

We propose bulk duals for certain coarse-grained entropies of boundary regions. The `one-point entropy' is defined in the conformal field theory by maximizing the entropy in a domain of dependence while fixing the one-point functions. We conjecture that this is dual to the area of the edge of the region causally accessible to the domain of dependence (i.e. the `causal holographic information' of Hubeny and Rangamani). The `future one-point entropy' is defined by generalizing this conjecture to future domains of dependence and their corresponding bulk regions. We show that the future one-point entropy obeys a nontrivial second law. If our conjecture is true, this answers the question "What is the field theory dual of Hawking's area theorem?"Comment: 43 pages, 9 figures. v3: minor changes suggested by referee v2: added a few additional reference

The principles of integration in urban transport strategies

Integration as a principle in urban transport policy is frequently advocated but rarely defined. We suggest a range of types of integration, and highlight the problems in developing an effective integrated strategy, given the number of variables involved. We argue that integration should be designed to serve agreed objectives of transport policy, rather than being an objective in its own right. We then consider the principles for designing an effective integrated strategy. We define the concept of synergy, which is often advocated as a benefit of integration, and discuss whether it, and other aggregation benefits short of true synergy, are achievable. We then consider the alternative approach of using integration to overcome barriers, an approach which is likely to be in conflict with pursuit of synergy, but more likely to lead to readily implemented strategies. We then review a number of examples where these principles have been applied, and investigate them to assess whether synergy has been demonstrated. Generally we find little evidence of synergy in outcome indicators. We conclude with some more general guidance on approaches to integration

The Bosnian Muslims and the Irish Perspective

The conflict in Bosnia-Herzegovina can be understood in multiple ways, however, the focus of this paper is to examine the perspective of Ireland on the Bosnian Muslims at different levels of society--ranging from the population to international level--from June 01, 1992 to January 31, 1996. Through an analysis of letters to the editor in The Irish Times, parliamentary debate transcripts, and the Barbara Sloan European Union Document Collection located at the University of Pittsburgh\u27s Hillman Library, I have been able to reveal how complex perspectives within a state on a particular issue can be, and how they can vary between states

Hedonic Prices and Implicit Markets: Estimating Marginal Willingness to Pay for Differentiated Products Without Instrumental Variables

The hedonic model of Rosen (1974) has become a workhorse for valuing the characteristics of differentiated products despite a number of well-documented econometric problems. For example, Bartik (1987) and Epple (1987) each describe a source of endogeneity in the second stage of Rosen's procedure that has proven difficult to overcome. In this paper, we propose a new approach for recovering the marginal willingness-to-pay function that altogether avoids these endogeneity problems. Applying this estimator to data on large changes in violent crime rates, we find that marginal willingness-to-pay increases by ten cents with each additional violent crime per 100,000 residents.

Spacelab Data Processing Facility (SLDPF) quality assurance expert systems development

The Spacelab Data Processing Facility (SLDPF) is an integral part of the Space Shuttle data network for missions that involve attached scientific payloads. Expert system prototypes were developed to aid in the performance of the quality assurance function of the Spacelab and/or Attached Shuttle Payloads processed telemetry data. The Spacelab Input Processing System (SIPS) and the Spacelab Output Processing System (SOPS), two expert systems, were developed to determine their feasibility and potential in the quality assurance of processed telemetry data. The capabilities and performance of these systems are discussed