Surface phase transitions of adsorbed collector molecules as revealed by in-situ FT-IR/IRS spectroscopy

Journal ArticleIt has been shown by inspection of FT-IR/IRS spectra that the alkyl conformation of adsorbed collectors can be determined. The conformations of adsorbed collectors were interpreted in terms of bulk solution phases of the collectors. Such analysis was performed on the CaF2/oleate, A1203/SDS and KC1/octylamine flotation systems. It was found that each system has unique conformational behavior, depending upon variables such as adsorption density and temperature. For the CaF^oleate system, the adsorbed species resemble a coagel at room temperature. Raising the temperature of the chemisorbed species to 40° C caused the adsorbed oleate to become micellar. Liquid crystalline-adsorbed species were observed in the A12C»3/dodecylsulfate system at low adsorption densities. As the adsorption density increased, the adsorbed species resembled a coagel/liquid crystal mixture. In all cases, octylamine adsorbed by KC1 appeared micellar in nature

Internal reflection spectroscopy for FTIR analysis of carboxylate adsorption by semi-soluble salt minerals

Journal ArticleFlotation chemists have long sought to unravel the mechanisms of carboxylate collector adsorption by semi-soluble salt minerals. In particular, the adsorption density of adsorbed carboxylates, adsorption kinetics, speciation (chemisorbed, physisorbed), orientation, and conformation are of interest. Given the importance of these phenomena in establishing the hydrophobic state at a mineral surface, in-situ surface spectroscopy studies certainly are justified. Such measurements traditionally have been difficult to perform. However, beginning in the late 1980s, a Fourier transform infrared (FTIR) spectroscopic technique was developed that has allowed for the acquisition of a wealth of detailed information concerning carboxylate adsorption in semi-soluble salt flotation systems. The technique, internal reflection spectroscopy using single-crystal internal reflection elements, is reviewed, as it applies to semi-soluble salt flotation systems

Infrared spectroscopy for in-situ characterization of surface reactions

Journal ArticleInfrared spectroscopy has been one of the most useful experimental techniques for the analysis of surface reactions in flotation systems, including surface alteration and surfactant adsorption reactions. With the advent of Fourier transform infrared (FTIR) spectrometers, many low-energy throughput IR experimental techniques previously unused by flotation researchers have come into greater use. In particular, internal reflection spectroscopy (IRS) is the most often used technique for in-situ studies. To date, three IRS methods have been used for in-situ measurements. These techniques are evaluated for use in characterization of surface reactions in flotation systems. The method using mineral crystals as reactive internal reflection elements (IRE) is of particular interest because it allows surfactant adsorption densities to be calculated directly from in-situ spectral data in real time

Quantitative in-situ analysis of collector adsorption reactions by FTIR internal reflection spectroscopy

Journal ArticleResearchers in flotation chemistry have long sought to describe collector adsorption phenomena in greater detail including kinetics, orientation and interactions between adsorbed species. Now a quantitative description can be made in-situ using FTIR internal reflection spectroscopy. Details of the adsorption process at the surface of mineral crystals, machined into internal reflection elements, can be described from first principles, provided the refractive indices, incident angle, and wavelength of light are known

In-Situ Cure Monitoring of the Immidization Reaction of PMR-15

Glass fiber reinforced polymer composites are becoming widely used in industry. With this increase in production, an in-situ method of quality control for the curing of the polymer is desirable. This would allow for the production of high-quality parts having more uniform properties.' Recently, in-situ fiber optic monitoring of polymer curing has primarily focused on epoxy resins and has been performed by Raman or fluorescence methods. In addition, some infrared (IR) investigations have been performed using transmission or ATR cells. An alternate IR approach involves using optical fibers as a sensor by utilizing evanescent wave spectroscopy

Dust emission at 8-mic and 24-mic as Diagnostics of HII Region Radiative Transfer

We use the Spitzer SAGE survey of the Magellanic Clouds to evaluate the relationship between the 8-mic PAH emission, 24-mic hot dust emission, and HII region radiative transfer. We confirm that in the higher-metallicity Large Magellanic Cloud, PAH destruction is sensitive to optically thin conditions in the nebular Lyman continuum: objects identified as optically thin candidates based on nebular ionization structure show 6 times lower median 8-mic surface brightness (0.18 mJy arcsec^-2) than their optically thick counterparts (1.2 mJy arcsec^-2). The 24-mic surface brightness also shows a factor of 3 offset between the two classes of objects (0.13 vs 0.44 mJy arcsec^-2, respectively), which is driven by the association between the very small dust grains and higher density gas found at higher nebular optical depths. In contrast, PAH and dust formation in the low-metallicity Small Magellanic Cloud is strongly inhibited such that we find no variation in either 8-mic or 24-mic emission between our optically thick and thin samples. This is attributable to extremely low PAH and dust production together with high, corrosive UV photon fluxes in this low-metallicity environment. The dust mass surface densities and gas-to-dust ratios determined from dust maps using Herschel HERITAGE survey data support this interpretation.Comment: Accepted to ApJ, May 15, 2017. 10 pages, 9 figure

A New Radioligand Binding Assay to Measure the Concentration of Drugs in Rodent Brain Ex Vivo

ABSTRACT We have developed a new radioligand binding assay method to measure the concentration of nonradiolabeled drugs in the brain ex vivo. This new method fuses the concepts of standard competition and saturation binding assays and uses a transformed version of the Cheng-Prusoff equation (Biochem Pharmacol 22: 3099 -3108, 1973) to calculate the drug concentration. After testing the validity of this method, we demonstrated its utility by measuring the brain concentration of sazetidine-A, a newly developed nicotinic receptor ligand, and its elimination rate after a single subcutaneous administration. Our results indicate that sazetidine-A reaches brain concentrations that are known to occupy and desensitize the majority of neuronal nicotinic acetylcholine receptor binding sites. Furthermore, using this method, we estimated the half-life of sazetidine-A in the rat brain to be ϳ65 min. It is important to note that the method described here to measure sazetidine-A in brain should be generalizable to other drugs acting at any receptor that can be reliably measured with a radiolabeled ligand

Biodiversity comparison among phylogenetic diversity metrics and between three North American prairies

Protection of Earth’s ecosystems requires identification of geographical areas of greatest biodiversity. Assessment of biodiversity begins with knowledge of the evolutionary histories of species in a geographic area. Multiple phylogenetic diversity (PD) metrics have been developed to describe biodiversity beyond species counts, but sufficient empirical studies, particularly at fine phylogenetic scales, have not been conducted to provide conservation planners with evidence for incorporating PD metrics into selection of priority regions. We review notable studies that are contributing to a growing database of empirical results, we report on the effect of using high-throughput sequencing to estimate the phylogenies used to calculate PD metrics, and we discuss difficulties in selecting appropriate diversity indices. We focused on two of the most speciose angiosperm families in prairies—Asteraceae and Fabaceae—and compared 12 PD metrics and four traditional measures of biodiversity between three North American prairie sites. The varying results from the literature and from the current data reveal the wide range of applications of PD metrics and the necessity for many more empirical studies. The accumulation of results from further investigations will eventually lead to a scientific understanding upon which conservation planners can make informed decisions about where to apply limited preservation funds