16 research outputs found

    Characterization of the Performance of a Turbocharger Centrifugal Compressor by Component Loss Contributions

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    The performance of an automotive turbocharger centrifugal compressor has been studied by developing a comprehensive one-dimensional (1D) code as verified through experimental results and a three-dimensional (3D) model. For 1D analysis, the fluid stream in compressor is modeled using governing gas dynamics equations and the loss mechanisms have been investigated and added to the numerical model. The objective is to develop and offer a 1D model which considers all loss mechanisms, slip, blockage and also predicts the surge margin and choke conditions. The model captures all features from inlet duct through to volute discharge. Performance characteristics are obtained using preliminary geometry and the blade characteristics. A 3D numerical model was also created and a viscous solver used for investigating the compressor characteristics. The numerical model results show good agreement with experimental data through compressor pressure ratio and efficiency. The effect of the main compressor dimensions on compressor performance has been investigated for wide operating range and the portions of each loss mechanism in the impeller. Higher pressure ratio is achievable by increasing impeller blade height at outlet, impeller blade angle on inlet, diffuser outlet diameter and by decreasing impeller shroud diameter at inlet and blade angle at outlet. These changes may cause unfavorable consequences such as lower surge margin or shorter operating range which should be compromised with favorable changes. At lower rotational speeds, impeller skin friction mainly impacts the performance and at higher rotational speeds, impeller diffusion, blade loading and recirculation losses are more important. The results allow the share of each loss mechanism to be quantified for different mass flow rates and rotational speed, shedding new light on which losses are most important for which conditions. For a turbocharger, which must operate over a wide range of conditions, these results bring new insight to engineers seeking to optimize the compressor design as part of an internal combustion engine system

    A Protein-Protein Interaction Map of the Trypanosoma brucei Paraflagellar Rod

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    We have conducted a protein interaction study of components within a specific sub-compartment of a eukaryotic flagellum. The trypanosome flagellum contains a para-crystalline extra-axonemal structure termed the paraflagellar rod (PFR) with around forty identified components. We have used a Gateway cloning approach coupled with yeast two-hybrid, RNAi and 2D DiGE to define a protein-protein interaction network taking place in this structure. We define two clusters of interactions; the first being characterised by two proteins with a shared domain which is not sufficient for maintaining the interaction. The other cohort is populated by eight proteins, a number of which possess a PFR domain and sub-populations of this network exhibit dependency relationships. Finally, we provide clues as to the structural organisation of the PFR at the molecular level. This multi-strand approach shows that protein interactome data can be generated for insoluble protein complexes

    Evidence for Loss of a Partial Flagellar Glycolytic Pathway during Trypanosomatid Evolution

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    Classically viewed as a cytosolic pathway, glycolysis is increasingly recognized as a metabolic pathway exhibiting surprisingly wide-ranging variations in compartmentalization within eukaryotic cells. Trypanosomatid parasites provide an extreme view of glycolytic enzyme compartmentalization as several glycolytic enzymes are found exclusively in peroxisomes. Here, we characterize Trypanosoma brucei flagellar proteins resembling glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK): we show the latter associates with the axoneme and the former is a novel paraflagellar rod component. The paraflagellar rod is an essential extra-axonemal structure in trypanosomes and related protists, providing a platform into which metabolic activities can be built. Yet, bioinformatics interrogation and structural modelling indicate neither the trypanosome PGK-like nor the GAPDH-like protein is catalytically active. Orthologs are present in a free-living ancestor of the trypanosomatids, Bodo saltans: the PGK-like protein from B. saltans also lacks key catalytic residues, but its GAPDH-like protein is predicted to be catalytically competent. We discuss the likelihood that the trypanosome GAPDH-like and PGK-like proteins constitute molecular evidence for evolutionary loss of a flagellar glycolytic pathway, either as a consequence of niche adaptation or the re-localization of glycolytic enzymes to peroxisomes and the extensive changes to glycolytic flux regulation that accompanied this re-localization. Evidence indicating loss of localized ATP provision via glycolytic enzymes therefore provides a novel contribution to an emerging theme of hidden diversity with respect to compartmentalization of the ubiquitous glycolytic pathway in eukaryotes. A possibility that trypanosome GAPDH-like protein additionally represents a degenerate example of a moonlighting protein is also discussed

    Measurement and CFD prediction of heat transfer in air-cooled disc-type electrical machines

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    Accurate thermal analysis of axial flux permanent magnet (AFPM) machines is crucial in predicting maximum power output. Stator convective heat transfer is one of the most important and least investigated heat transfer mechanisms and is the focus of this paper. Experimental measurements were undertaken using a thin-film electrical heating method, providing radially resolved steady state heat transfer data from an experimental rotor-stator system designed as a geometric mockup of a through-flow ventilated AFPM machine. The measurements are compared with computational fluid dynamics (CFD) simulations using both 2D axisymmetric and 3D models. These were found to give a conservative estimate of heat transfer, with inaccuracies near the edge and in the transitional flow regime. Predicted stator heat transfer was found to be relatively insensitive to the choice of turbulence model used in the CFD simulations

    The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease.

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    The Saccharomyces cerevisiae targets of rapamycin, TOR1 and TOR2, signal activation of cell growth in response to nutrient availability. Loss of TOR or rapamycin treatment causes yeast cells to arrest growth in early G1 and to express several other physiological properties of starved (G0) cells. As part of this starvation response, high affinity amino acid permeases such as the tryptophan permease TAT2 are targeted to the vacuole and degraded. Here we show that the TOR signalling pathway phosphorylates the Ser/Thr kinase NPR1 and thereby inhibits the starvation-induced turnover of TAT2. Overexpression of NPR1 inhibits growth and induces the degradation of TAT2, whereas loss of NPR1 confers resistance to rapamycin and to FK506, an inhibitor of amino acid import. NPR1 is controlled by TOR and the type 2A phosphatase-associated protein TAP42. First, overexpression of NPR1 is toxic only when TOR function is reduced. Secondly, NPR1 is rapidly dephosphorylated in the absence of TOR. Thirdly, NPR1 dephosphorylation does not occur in a rapamycin-resistant tap42 mutant. Thus, the TOR nutrient signalling pathway also controls growth by inhibiting a stationary phase (G0) programme. The control of NPR1 by TOR is analogous to the control of p70 s6 kinase and 4E-BP1 by mTOR in mammalian cells
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