In Vivo

In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research. We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation

Impact of pathological tumor stage for salvage radiotherapy after radical prostatectomy in patients with prostate-specific antigen < 1.0 ng/ml

<p>Abstract</p> <p>Background</p> <p>To evaluate prognostic factors in salvage radiotherapy (RT) for patients with pre-RT prostate-specific antigen (PSA) < 1.0 ng/ml.</p> <p>Methods</p> <p>Between January 2000 and December 2009, 102 patients underwent salvage RT for biochemical failure after radical prostatectomy (RP). Re-failure of PSA after salvage RT was defined as a serum PSA value of 0.2 ng/ml or more above the postradiotherapy nadir followed by another higher value, a continued rise in serum PSA despite salvage RT, or initiation of systemic therapy after completion of salvage RT. Biochemical relapse-free survival (bRFS) was estimated using the Kaplan-Meier method. Multivariate analysis was performed using the Cox proportional hazards regression model.</p> <p>Results</p> <p>The median follow-up period was 44 months (range, 11-103 months). Forty-three patients experienced PSA re-failure after salvage RT. The 4-year bRFS was 50.9% (95% confidence interval [95% CI]: 39.4-62.5%). In the log-rank test, pT3-4 (p < 0.001) and preoperative PSA (p = 0.037) were selected as significant factors. In multivariate analysis, only pT3-4 was a prognostic factor (hazard ratio: 3.512 [95% CI: 1.535-8.037], p = 0.001). The 4-year bRFS rates for pT1-2 and pT3-4 were 79.2% (95% CI: 66.0-92.3%) and 31.7% (95% CI: 17.0-46.4%), respectively.</p> <p>Conclusions</p> <p>In patients who have received salvage RT after RP with PSA < 1.0 ng/ml, pT stage and preoperative PSA were prognostic factors of bRFS. In particular, pT3-4 had a high risk for biochemical recurrence after salvage RT.</p

Outcomes after stereotactic body radiotherapy for lung tumors, with emphasis on comparison of primary lung cancer and metastatic lung tumors

BACKGROUND: The goal of this study was to determine the prognostic factors associated with an improved overall outcome after stereotactic body radiotherapy (SBRT) for primary lung cancer and metastatic lung tumors. METHODS: A total of 229 lung tumors in 201 patients were included in the study. SBRT of 45 Gy in 3 fractions, 48 Gy in 4 fractions, 60 Gy in 8 fractions or 60 Gy in 15 fractions was typically used to treat 172 primary lungs cancer in 164 patients and 57 metastatic lung tumors in 37 patients between January 2001 and December 2011. Prognostic factors for local control (LC) and overall survival (OS) were analyzed using a Cox proportional hazards model. RESULTS: The median biologically effective dose was 105.6 Gy based on alpha/beta = 10 (BED10). The median follow-up period was 41.9 months. The 3-year LC and OS rates were 72.5% and 60.9%, and the 5-year LC and OS rates were 67.8% and 38.1%, respectively. Radiation pneumonitis of grades 2, 3 and 5 occurred in 22 petients, 6 patients and 1 patient, respectively. Multivariate analyses revealed that tumor origin (primary lung cancer or metastatic lung tumor, p < 0.001), tumor diameter (p = 0.005), BED10 (p = 0.029) and date of treatment (p = 0.011) were significant independent predictors for LC and that gender (p = 0.012), tumor origin (p = 0.001) and tumor diameter (p < 0.001) were significant independent predictors for OS. CONCLUSIONS: SBRT resulted in good LC and tolerable treatment-related toxicities. Tumor origin and tumor diameter are significant independent predictors for both overall survival and local control

ALMA Observations of the Terahertz Spectrum of Sagittarius A*

We present Atacama Large Millimeter/submillimeter Array (ALMA) observations at 233, 678, and 870 GHz of the Galactic Center black hole, Sagittarius A*. These observations reveal a flat spectrum over this frequency range with spectral index α ≈ −0.3, where the flux density S ∝ ν α . We model the submillimeter and far-infrared spectrum with a one-zone synchrotron model of thermal electrons. We infer electron densities n = (2–5) × 106 cm−3, electron temperatures T e = (1–3) × 1011 K, and magnetic field strength B = 10–50 G. The parameter range can be further constrained using the observed quiescent X-ray luminosity. The flat submillimeter spectrum results in a high electron temperature and implies that the emitting electrons are efficiently heated. We also find that the emission is most likely optically thin at 233 GHz. These results indicate that millimeter and submillimeter wavelength very long baseline interferometry of Sgr A* including those of the Event Horizon Telescope should see a transparent emission region down to event horizon scales.Alexander von Humboldt foundation; NWO VICI grant [639.043.513]This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Suzaku Observation of Abell 1689: Anisotropic Temperature and Entropy Distributions Associated with the Large-Scale Structure

(Abridged) We present results of Suzaku observations of the intracluster medium (ICM) in Abell 1689, combined with complementary analysis of the SDSS data and weak and strong lensing analysis of Subaru/Suprime-Cam and HST/ACS observations. Faint X-ray emission from the ICM around the virial radius is detected at 4.0 sigma significance. We find anisotropic gas temperature and entropy distributions in cluster outskirts correlated with large-scale structure of galaxies. The high temperature and entropy region in the northeastern (NE) outskirts is connected to an overdense filamentary structure. The outskirt regions in contact with low density void environments have low gas temperatures and entropies, deviating from hydrostatic equilibrium. These results suggest that thermalization of the ICM occurs faster along the filamentary structures than the void regions. A joint X-ray and lensing analysis shows that the hydrostatic mass is $\sim60-90$ of spherical lensing one but comparable to a triaxial halo mass within errors in 0.6r_{2500} \simlt r \simlt 0.8r_{500}, and that it is significantly biased as low as \simlt60% within $0.4r_{2500}$, irrespective of mass models. The thermal gas pressure within $r_{500}$ is, at most, $\sim50$--60% of the total pressure to balance fully the gravity of the spherical lensing mass, and $\sim30$--40% around the virial radius. Although these constitute lower limits when one considers the possible halo triaxiality, these small relative contributions of thermal pressure would require additional sources of pressure, such as bulk and/or turbulent motions.Comment: 24 pages, 15 figures, 9 tables. Accepted for publication in Ap