An HST/COS Survey of the Low-Redshift IGM. I. Survey, Methodology, & Overall Results

We use high-quality, medium-resolution {\it Hubble Space Telescope}/Cosmic Origins Spectrograph (\HST/COS) observations of 82 UV-bright AGN at redshifts $z_{AGN}<0.85$ to construct the largest survey of the low-redshift intergalactic medium (IGM) to date: 5343 individual extragalactic absorption lines in HI and 25 different metal-ion species grouped into 2610 distinct redshift systems at $z_{abs}<0.75$ covering total redshift pathlengths $\Delta z_{HI}=21.7$ and $\Delta z_{OVI}=14.5$. Our semi-automated line-finding and measurement technique renders the catalog as objectively-defined as possible. The cumulative column-density distribution of HI systems can be parametrized $dN(>N)/dz=C_{14}(N/10^{14} cm^{-2})^{-(\beta-1)}$, with $C_{14}=25\pm1$ and $\beta=1.65\pm0.02$. This distribution is seen to evolve both in amplitude, $C_{14}\sim(1+z)^{2.0\pm0.1}$, and slope $\beta(z)=1.73-0.26 z$ for $z<0.47$. We observe metal lines in 427 systems, and find that the fraction of IGM absorbers detected in metals is strongly dependent on N_{HI}. The distribution of OVI absorbers appear to evolve in the same sense as the Lya forest. We calculate contributions to $\Omega_b$ from different components of the low-$z$ IGM and determine the Lya decrement as a function of redshift. IGM absorbers are analyzed via a two-point correlation function (TPCF) in velocity space. We find substantial clustering of \HI\ absorbers on scales of $\Delta v=50-300$ km/s with no significant clustering at $\Delta v>1000$ km/s. Splitting the sample into strong and weak absorbers, we see that most of the clustering occurs in strong, $N_{HI}>10^{13.5} cm^{-2}$, metal-bearing IGM systems. The full catalog of absorption lines and fully-reduced spectra is available via MAST as a high-level science product at http://archive.stsci.edu/prepds/igm/.Comment: This is the accepted version (v3) of the paper. Previous versions (July 2015 and Feb. 2014) should be replaced by this one. In particular, please note that the associated MAST high-level-science product has been updated to reflect the of the final state of the paper. It is available at: http://archive.stsci.edu/prepds/igm

Silencing of PINK1 Expression Affects Mitochondrial DNA and Oxidative Phosphorylation in DOPAMINERGIC Cells

Background: Mitochondrial dysfunction has been implicated in the pathogenesis of Parkinson's disease (PD). Impairment of the mitochondrial electron transport chain (ETC) and an increased frequency in deletions of mitochondrial DNA (mtDNA), which encodes some of the subunits of the ETC, have been reported in the substantia nigra of PD brains. The identification of mutations in the PINK1 gene, which cause an autosomal recessive form of PD, has supported mitochondrial involvement in PD. The PINK1 protein is a serine/threonine kinase localized in mitochondria and the cytosol. Its precise function is unknown, but it is involved in neuroprotection against a variety of stress signalling pathways.Methodology/Principal Findings: In this report we have investigated the effect of silencing PINK1 expression in human dopaminergic SH-SY5Y cells by siRNA on mtDNA synthesis and ETC function. Loss of PINK1 expression resulted in a decrease in mtDNA levels and mtDNA synthesis. We also report a concomitant loss of mitochondrial membrane potential and decreased mitochondrial ATP synthesis, with the activity of complex IV of the ETC most affected. This mitochondrial dysfunction resulted in increased markers of oxidative stress under basal conditions and increased cell death following treatment with the free radical generator paraquat.Conclusions: This report highlights a novel function of PINK1 in mitochondrial biogenesis and a role in maintaining mitochondrial ETC activity. Dysfunction of both has been implicated in sporadic forms of PD suggesting that these may be key pathways in the development of the disease

Molecular approaches to trematode systematics: 'best practice' and implications for future study

To date, morphological analysis has been the cornerstone to trematode systematics. However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the ‘best molecular practice’ for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work. Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient ‘useful’ comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics

Creative Thinking and Modelling for the Decision Support in Water Management

This paper reviews the state of art in knowledge and preferences elicitation techniques. The purpose of the study was to evaluate various cognitive mapping techniques in order to conclude with the identification of the optimal technique for the NetSyMod methodology. Network Analysis Creative System Modelling (NetSyMod) methodology has been designed for the improvement of decision support systems (DSS) with respect to the environmental problems. In the paper the difference is made between experts and stakeholders knowledge and preference elicitation methods. The suggested technique is very similar to the Nominal Group Techniques (NGT) with the external representation of the analysed problem by means of the Hodgson Hexagons. The evolving methodology is undergoing tests within several EU-funded projects such as: ITAES, IISIM, NostrumDSS

Transient exposure to rotenone causes degeneration and progressive parkinsonian motor deficits, neuroinflammation, and synucleinopathy

Abstract Individuals with Parkinson’s disease (PD) typically receive a diagnosis once they have developed motor symptoms, at which point there is already significant loss of substantia nigra dopamine neurons, α-synuclein accumulation in surviving neurons, and neuroinflammation. Consequently, the point of clinical presentation may be too late to initiate disease-modifying therapy. In contrast to this clinical reality, animal models often involve acute neurodegeneration and potential therapies are tested concurrently or shortly after the pathogenic insult has begun rather than later when diagnostic clinical symptoms emerge. Therefore, we sought to develop a model that reflects the clinical situation more accurately. Middle-aged rats (7–9 months-old) received a single daily intraperitoneal injection of rotenone for 5 consecutive days and were observed over the next 8–9 months. Rotenone-treated rats showed transient motor slowing and postural instability during exposure but recovered within 9 days of rotenone cessation. Rats remained without behavioral deficits for 3–4 months, then developed progressive motor abnormalities over the ensuing months. As motor abnormalities began to emerge 3 months after rotenone exposure, there was significant loss of nigral dopaminergic neurons and significant microglial activation. There was delayed accumulation of α-synuclein in neurons of the substantia nigra and frontal cortex, which was maximal at 9 months post-rotenone. In summary, a brief temporally-remote exposure to rotenone causes delayed and progressive behavioral and neuropathological changes similar to Parkinson’s disease. This model mimics the human clinical situation, in which pathogenesis is well-established by the time diagnostic motor deficits appear. As such, this model may provide a more relevant experimental system in which to test disease-modifying therapeutics

Characterization and Inhibition of a Nickel-Alpha-Hydroxyoxime (LIX 63) Salt Precipitate Formed under Proposed Commercial Operating Conditions

As a prospective commercial solvent extraction (SX) process, laboratory-scale continuous tests were recently undertaken to assess the use of a solution of LIX 63 hydroxyoxime (“hydroxyoxime”) and Versatic 10 to kinetically separate cobalt from a nickel-rich sulfate solution while simultaneously rejecting manganese and magnesium. A material quantity of blue precipitate observed in the strip stage cells during decommissioning has been identified as the sulfate salt of the nickel-trishydroxyoxime complex. As precipitation during SX is undesirable, the effect of various operating parameters on precipitate formation has been investigated. Minimizing aqueous nickel and/or sulfuric acid concentration and/or increasing organic polarity can overcome this problem. Where it forms, hydroxyoxime and nickel can be recovered from the precipitate by redissolution under suitable (e.g., low acid) operating conditions. The nitrate salt counterpart of this sulfate precipitate has beencrystallographically characterized using a short chained (C8) analogue of LIX 63 hydroxyoxime, revealing the coordination of three neutral hydroxyoxime ligands around nickel, forming a monomeric coordination complex cation counterbalanced by nitrate anions