Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2

A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way DNA junctions. The activity cuts with high specificity a synthetic four-way junction containing a 12 bp core of homologous sequences but has no activity on another four-way junction (with a fixed crossover point), a three-way junction, linear duplex DNA or duplex DNA containing six mismatched nucleotides in the centre. The major cleavage sites map as single nicks in the vicinity of the crossover point, 3' of a thymidine residue. These data indicate that the activity has a strong DNA structure selectivity as well as a limited sequence preference; features similar to the Holliday junction resolving enzymes RuvC of Escherichia coli and the mitochondrial CCE1 (cruciform-cuttingenzyme 1) of Saccharomyces cerevisiae. A putative homologue of CCE1 in S.pombe (YDC2_SCHPO) has been identified through a search of the sequence database. The open reading frame of this gene has been cloned and the encoded protein, YDC2, expressed in E.coli . The purified recombinant YDC2 exhibits Holliday junction resolvase activity and is, therefore, a functional S.pombe homologue of CCE1. The resolvase YDC2 shows the same substrate specificity and produces identical cleavage sites as the activity obtained from S. pombe cells. Both YDC2 and the cellular activity cleave Holliday junctions in both orientations to give nicks that can be ligated in vitro. The partially purified Holliday junction resolving enzyme in fission yeast is biochemically indistinguishable from recombinant YDC2 and appears to be the same protein

Influence of single-neutron stripping on near-barrier ⁶He+²⁰⁸Pb and ⁸He+²⁰⁸Pb elastic scattering

The influence of single-neutron stripping on the near-barrier elastic scattering angular distributions for the 6,8He+208Pb systems is investigated through coupled reaction channels (CRC) calculations fitting recently published data to explore the differences in the absorptive potential found in the scattering of these two neutron-rich nuclei. The inclusion of the coupling reduces the elastic cross section in the Coulomb-nuclear interference region for 8He scattering, whereas for 6He its major impact is on the large-angle elastic scattering. The real and imaginary dynamic polarization potentials are obtained by inverting the CRC elastic scattering S-matrix elements. These show that the main absorptive features occur between 11 and 12 fm for both projectiles, while the attractive features are separated by about 1 fm, with their main structures occurring at 10.5 fm for 6He and 11.5 fm for 8He

Concept definition study for recovery of tumbling satellites. Volume 1: Executive summary, study results

The first assessment is made of the design requirements and conceptual definition of a front end kit to be transported on the currently defined Orbital Maneuvering Vehicle (OMV) and the Space Transportation System Shuttle Orbiter, to conduct remote, teleoperated recovery of disabled and noncontrollable, tumbling satellites. Previous studies did not quantify the dynamic characteristics of a tumbling satellite, nor did they appear to address the full spectrum of Tumbling Satellite Recovery systems requirements. Both of these aspects are investigated with useful results

Concept definition study for recovery of tumbling satellites. Volume 2: Supporting research and technology report

A number of areas of research and laboratory experiments were identified which could lead to development of a cost efficient remote, disable satellite recovery system. Estimates were planned of disabled satellite motion. A concept is defined as a Tumbling Satellite Recovery kit which includes a modular system, composed of a number of subsystem mechanisms that can be readily integrated into varying combinations. This would enable the user to quickly configure a tailored remote, disabled satellite recovery kit to meet a broad spectrum of potential scenarios. The capability was determined of U.S. Earth based satellite tracking facilities to adequately determine the orientation and motion rates of disabled satellites

Coupled-channels effects in elastic scattering and near-barrier fusion induced by weakly bound nuclei and exotic halo nuclei

The influence on fusion of coupling to the breakup process is investigated for reactions where at least one of the colliding nuclei has a sufficiently low binding energy for breakup to become an important process. Elastic scattering, excitation functions for sub-and near-barrier fusion cross sections, and breakup yields are analyzed for $^{6,7}$Li+$^{59}$Co. Continuum-Discretized Coupled-Channels (CDCC) calculations describe well the data at and above the barrier. Elastic scattering with $^{6}$Li (as compared to $^{7}$Li) indicates the significant role of breakup for weakly bound projectiles. A study of $^{4,6}$He induced fusion reactions with a three-body CDCC method for the $^6$He halo nucleus is presented. The relative importance of breakup and bound-state structure effects on total fusion is discussed.Comment: 29 pages, 9 figure

Three birds with one stone: Tidal wetland restoration, carbon sequestration, and enhancing resilience to rising sea levels in the Snohomish River Estuary, Washington

Recent attention has focused on exploring the carbon storage and sequestration values of tidal wetlands to mitigate greenhouse gas emissions. Efforts are now underway to develop the tools and refine the science needed to bring carbon markets to bear on tidal wetland restoration activities. Effective restoration not only maximizes carbon storage in former tidal wetlands but also, through the accumulation of organic and mineral matter, enhances these systems’ resilience to rising sea levels. To this end, this project focuses on the Snohomish River estuary of the Puget Sound, Washington, which offers a continuum of diked and un-diked wetlands including seasonal floodplains, open mudflats, mature and tidal forests, and salt marsh habitats. In addition, there is strong restoration potential in a suite of ongoing and proposed projects. We report here on the carbon storage pools, long-term sediment accretion rates (100 years), and estimated rates of carbon storage, derived from sediment cores collected at representative sites within the Snohomish estuary during the spring and summer of 2013. We found that natural wetlands (open to tidal exchange and riverine inputs) were accreting at rates that equaled or exceeded current rates of eustatic sea level rise, while formerly, or currently diked wetlands (closed to such exchanges and inputs) revealed marked evidence of subsidence. Restored sites showed evidence of both high rates of sediment accretion (1.61 cm/year) and carbon storage (352 g C/m2/year)

Reaction channel contributions to the helion optical potential

Background: The well-established coupled channel and coupled reaction channel processes contributing to direct reactions make particular contributions to elastic scattering that are absent from local density folding models. Very little has been established concerning the contribution of these processes to the optical model potentials (OMPs) for 3He scattering. For studying such processes, spin-saturated closed shell nuclei such as 16O and 40Ca are particularly suitable target nuclei and the (3He, 4He) reaction is easily handled within conventional reaction theory because it avoids complications such as breakup.Purpose: To establish and characterize the contribution to the 3He-nucleus interaction generated by coupling to neutron pickup (outgoing 4He) channels; also to study the contribution of collective states and identify effects of dynamical nonlocality from these couplings.Methods: Coupled reaction channel (CRC) calculations, including coupling to collective states, will provide the elastic channel S-matrix Sl j resulting from the included processes. Inversion of Sl j will produce the local potential that yields, in a single channel calculation, the elastic scattering observables from the coupled channel calculation. Subtracting the bare potential from the CRC calculations yields a local and l-independent representation of the dynamical polarization potential (DPP). From the DPPs, because of a range of combinations of channel couplings, the influence of dynamically generated nonlocality can be identified.Results: Coupling to 4He channels systematically induces repulsion and absorption in the 3He OMP and also a reduction in the rms radius of the real part. The repulsion and absorption is less for 208Pb than for the lighter target nuclei although the qualitative effects, including the general undularity of the DPPs, are similar for all cases; therefore coupling to these channels cannot be represented by renormalizing folding model potentials. Evidence is presented for substantial dynamical nonlocality of the induced DPPs; for 40Ca this modifies direct reaction angular distributions. The local equivalent DPPs for individual couplings cannot be added to give the overall DPP for the complete set of couplings. For the 208Pb case, channel coupling reduces the reaction cross section although it increases it for 16O, with 40Ca an intermediate case. Conclusions: The DPPs established here strongly challenge the notion that folding models, in particular local density models, provide a satisfactory description of elastic scattering of 3He from nuclei. Coupling to neutron pickup channels induces dynamical nonlocality in the 3He OMP with implications for direct reactions involving 3He. Departures from a smooth radial form for the 3He OMP should be apparent in good fits to suitable elastic scattering data