The Dipole Observed in the COBE DMR Four-Year Data

The largest anisotropy in the cosmic microwave background (CMB) is the $\approx 3$ mK dipole assumed to be due to our velocity with respect to the CMB. Using the four year data set from all six channels of the COBE Differential Microwave Radiometers (DMR), we obtain a best-fit dipole amplitude $3.358 \pm 0.001 \pm 0.023$ mK in the direction $(l,b)=(264\deg.31 \pm 0\deg.04 \pm 0\deg.16, +48\deg.05 \pm 0\deg.02 \pm 0\deg.09)$, where the first uncertainties are statistical and the second include calibration and combined systematic uncertainties. This measurement is consistent with previous DMR and FIRAS resultsComment: New and improved version; to be published in ApJ next mont

Vijftig jaar monitoring en beheer van de Friese en Groninger kwelderwerken: 1960-2009

Dit WOt-werkdocument is een update van het kwelderboek uit 2001 en gaat ook over de bescherming door de kwelderwerken tegen de Allerheiligenvloed van 2006, over de Kaderrichtlijn Water en over de Sylt Conferentie van 2010. In 2007 verscheen in de WOT IN serie al een deel over Monitoring van kwelders in de Waddenzee, met beheermaatregelen voor alle kwelders (www.waddenzee.nl/Kwelders.1982.0.html). In de publicaties is ook aandacht voor beweiding, vegetatiekaarten, veroudering van de vegetatie, zeegras, grondwerk, ontwatering, en duurzaamheid van de rijshoutdammen

Correlated Errors in the COBE DMR Sky Maps

The {\it COBE} DMR sky maps contain low-level correlated noise. We obtain estimates of the amplitude and pattern of the correlated noise from three techniques: angular averages of the covariance matrix, Monte Carlo simulations of two-point correlation functions, and direct analysis of the DMR maps. The results from the three methods are mutually consistent. The noise covariance matrix of a DMR sky map is diagonal to an accuracy of better than 1\%. For a given sky pixel, the dominant noise covariance occurs with the ring of pixels at an angular separation of $60 \deg$ due to the $60 \deg$ separation of the DMR horns. The mean covariance at $60 \deg$ is $0.45\% ^{+0.18}_{-0.14}$ of the mean variance. Additionally, the variance in a given pixel is $0.7\%$ greater than would be expected from a single beam experiment with the same noise properties. Auto-correlation functions suffer from a $\sim 1.5\; \sigma$ positive bias at $60 \deg$ while cross-correlations have no bias. Published {\it COBE} DMR results are not significantly affected by correlated noise. COBE pre-print 94-Comment: 11 pages + 3 figures, post-script fil

Localization of a 64-kDa phosphoprotein in the lumen between the outer and inner envelopes of pea chloroplasts

The identification and localization of a marker protein for the intermembrane space between the outer and inner chloroplast envelopes is described. This 64-kDa protein is very rapidly labeled by [γ-32P]ATP at very low (30 nM) ATP concentrations and the phosphoryl group exhibits a high turnover rate. It was possible to establish the presence of the 64-kDa protein in this plastid compartment by using different chloroplast envelope separation and isolation techniques. In addition comparison of labeling kinetics by intact and hypotonically lysed pea chloroplasts support the localization of the 64-kDa protein in the intermembrane space. The 64-kDa protein was present and could be labeled in mixed envelope membranes isolated from hypotonically lysed plastids. Mixed envelope membranes incorporated high amounts of 32P from [γ-32P]ATP into the 64-kDa protein, whereas separated outer and inner envelope membranes did not show significant phosphorylation of this protein. Water/Triton X-114 phase partitioning demonstrated that the 64-kDa protein is a hydrophilic polypeptide. These findings suggest that the 64-kDa protein is a soluble protein trapped in the space between the inner and outer envelope membranes. After sonication of mixed envelope membranes, the 64-kDa protein was no longer present in the membrane fraction, but could be found in the supernatant after a 110000 × g centrifugation

Friese en Groninger kwelderwerken : monitoring en beheer 1960-2010

Zowel in nationaal als in trilateraal verband geldt als één van de ecologische doelen voor de Waddenzee een zo groot en natuurlijk mogelijk areaal aan kwelders. Actief ingrijpen om bestaande kwelders in stand te houden dient op een zo natuurlijk mogelijke wijze plaats te vinden. In de kwelderwerken en zomerpolders langs Friese en Groninger vastelandskust is een omslag in beheer ingezet richting duurzamer en minder kunstmatig. Langetermijnmonitoring van onder meer de hoogte- en vegetatieontwikkeling begeleidt deze verandering en dient ook om te zien of de meer natuurlijke wijze van beheer zich verdraagt met de effecten van zeespiegelstijging. De resultaten worden jaarlijks op www.waddenzee.nl gepubliceerd en zijn verder onder andere ook input voor de vijfjaarlijkse Quality Status Reports in het kader van de trilaterale samenwerking tussen de Wadddenzee-landen. De Waddenzee is het belangrijkste gebied voor éénjarige pioniervegetaties van Zeekraal. Deze pionierzone is de overgang van wadplaten naar kwelder en beschermt de hoger gelegen kwelderzones. Door opslibbing worden kwelders hoger, waarbij de vegetatie door successie verandert. De vegetatie ontwikkelt zich tijdens dat proces tot een eindstadium of climaxbegroeiing. De biodiversiteit neemt sterk af als een kwelder in zijn eindfase komt door veroudering met als eindstadium een soortenarme vegetatie van Zeekweek. Begreppeling versnelt de veroudering van de kwelderzone. Beweiding stelt de ontwikkeling van een climaxvegetatie uit. De ideale natuurlijke situatie zou cyclische successie zijn, daarbij zijn aangroei en afslag van kwelders in evenwicht. De kwaliteit van kwelders kan worden verbeterd door de variatie aan hoogtezones, geomorfologische vormen (groene stranden, slufters, zandige kwelders, kleiige kwelders) en beheervormen (beweide en onbeweide kwelders) te behouden of te herstelle

A guanosine 5′-triphosphate-dependent protein kinase is localized in the outer envelope membrane of pea chloroplasts

A guanosine 5-triphosphate (GTP)-dependent protein kinase was detected in preparations of outer chloroplast envelope membranes of pea (Pisum sativum L.) chloroplasts. The protein-kinase activity was capable of phosphorylating several envelope-membrane proteins. The major phosphorylated products were 23- and 32.5-kilo-dalton proteins of the outer envelope membrane. Several other envelope proteins were labeled to a lesser extent. Following acid hydrolysis of the labeled proteins, most of the label was detected as phosphoserine with only minor amounts detected as phosphothreonine. Several criteria were used to distinguish the GTP-dependent protein kinase from an ATP-dependent kinase also present in the outer envelope membrane. The ATP-dependent kinase phosphorylated a very different set of envelope-membrane proteins. Heparin inhibited the GTP-dependent kinase but had little effect upon the ATP-dependent enzyme. The GTP-dependent enzyme accepted phosvitin as an external protein substrate whereas the ATP-dependent enzyme did not. The outer membrane of the chloroplast envelope also contained a phosphotransferase capable of transferring labeled phosphate from [-32P]GTP to ADP to yield (-32P]ATP. Consequently, addition of ADP to a GTP-dependent protein-kinase assay resulted in a switch in the pattern of labeled products from that seen with GTP to that typically seen with ATP

Metabolomics demonstrates divergent responses of two Eucalyptus species to water stress

Past studies of water stress in Eucalyptus spp. generally highlighted the role of fewer than five “important” metabolites, whereas recent metabolomic studies on other genera have shown tens of compounds are affected. There are currently no metabolite profiling data for responses of stress-tolerant species to water stress. We used GC–MS metabolite profiling to examine the response of leaf metabolites to a long (2 month) and severe (Ψpredawn < −2 MPa) water stress in two species of the perennial tree genus Eucalyptus (the mesic Eucalyptus pauciflora and the semi-arid Eucalyptus dumosa). Polar metabolites in leaves were analysed by GC–MS and inorganic ions by capillary electrophoresis. Pressure–volume curves and metabolite measurements showed that water stress led to more negative osmotic potential and increased total osmotically active solutes in leaves of both species. Water stress affected around 30–40% of measured metabolites in E. dumosa and 10–15% in E. pauciflora. There were many metabolites that were affected in E. dumosa but not E. pauciflora, and some that had opposite responses in the two species. For example, in E. dumosa there were increases in five acyclic sugar alcohols and four low-abundance carbohydrates that were unaffected by water stress in E. pauciflora. Re-watering increased osmotic potential and decreased total osmotically active solutes in E. pauciflora, whereas in E. dumosa re-watering led to further decreases in osmotic potential and increases in total osmotically active solutes. This experiment has added several extra dimensions to previous targeted analyses of water stress responses in Eucalyptus, and highlights that even species that are closely related (e.g. congeners) may respond differently to water stress and re-waterin