The Effect of Clerodendrum Myricoides Aqueous Extract on Blood, Liver and Kidney Tissues of Mice

Clerodendrum myricoides is a plant traditionally used for its medicinal value in many parts of the world including Ethiopia. Several human defects are treated in different regions of Ethiopia using the roots, leaves, twigs, fruits and root barks of Clerodendrum myricoides species. The objective of the present study is to investigate the effect of the aqueous extract of Clerodendrum myricoides root on different tissue of mice in chronic administration. The root of the plant were collected in Bale area, Ethiopia dried and crushed into powder and soaked in water to yield aqueous extract. The extract was then administered to mice at doses of 100 and 400mg/kg bw/day for six weeks and 1134mg/kg bw/day (LD50) once. The 400mg/kg bw/day and LD50 treated mice showed hypoactivity (abnormally diminished activity), grooming, prostration (submissively prone position), piloroerection (elevation of hairs) and irritation, while 100mg/kg bw/day treated mice showed no behavioral changes. The dose of 100mg/kg bw/day produced significant weight gain, while 400mg/kg bw/day produced significant weight lost in chronic administration. The extract increased only urea at the dose of 100mg/kg bw/day, whereas it increases most hematological and biochemical parameters at 400mg/kg bw/day. The extract, however did not show significant change in platelet, HGB and MCHC value at both doses. Several histopathological changes in liver and kidney were also observed at both doses of the extract. There were inflammation and hydropic degeneration of hepatocytes at both doses. The LD50 of the extract produced hemorrhages and congestion in the glomeruli of the kidney

Anomalous magnetic ordering in PrBa_2Cu_3O_{7-y} single crystals: Evidence for magnetic coupling between the Cu and Pr sublattices

In Al-free PrBa_2Cu_3O_{7-y} single crystals the kink in the temperature dependence of magnetic susceptibility chi_{ab}(T), connected with Pr antiferromagnetic ordering, disappears after field cooling (FC) in a field H || ab-plane. The kink in chi_c(T) remains unchanged after FC in H || c-axis. As a possible explanation, freezing of the Cu magnetic moments, lying in the ab-plane, caused by FC in H || ab, hinders their reorientation and, due to coupling between the Pr and Cu(2) sublattices, ordering of the Pr^{3+} moments. A field induced phase transition and a field dependence of the Pr^{3+} ordering temperature have been found for both H || c and H || ab.Comment: 11 pages (LaTex with elsart.sty), 5 EPS figs. Accepted to Physica

Husbandry practices and phenotypic characteristics of indigenous goat populations in Ethiopia.

Abstract: The present study was conducted with the objective to characterize the husbandry practices and phenotypic characteristics of mature Bati, Borana and Short-eared Somali goats kept under traditional management systems so that the information generated can be used in designing breeding programs. The study was covered five districts in four administrative zones of Ethiopia representing Bati (in Oromia Zone) and Kalu (in South Wollo Zone) for Bati goats; Yabello (in Borana Zone) for Borana goats and Shinille and Erer (in Siti Zone) for Short-Eared Somali goats. A total of 345 households interview was made and phenotypic measurements were taken on 601(468 females and 133 males) heads of adult goats with 4 pair of permanent incisors (PPI). However, because of difficulty of finding adequate number of 4PPI sample males, measurements were taken from 2PPI and above males. In this study, in number, goats accounted for 72.01, 50.93 and 47.38% of other livestock species in Siti, around Bati and Borana, respectively. The least square mean (±SE) of goat flock size (44.02±3.33) per household in Siti was significantly (p0.05),whereas Short-eared Somali goats remained significantly (p<0.05) lower for most of the body measurement characteristics. The canonical analysis done on phenotypic measurements also put Bati and Borana goats closer by discriminating Short-eared Somali goat populations. The similarities between Bati and Borena goats and significance divergence of Short-eared Somali goats in phenotypic measurements suggested that the need of further molecular characterization study to validate information from phenotypic characterization. Correlation coefficient was consistently highest between live weight and chest girth in both sexes across the goat populations. Hence, linear measurements could be valuable to estimate live body weight for those farm communities where sensitive weighing scales are not readily available

Immunogenicity and efficacy of non-adjuvant tissue culture-based rabies vaccine produced in Ethiopia

Rabies is 100% fatal, but it is preventable. More than 95% of human rabies cases occur in improperly treated individuals. This is partly due to the fact that modern post-exposure rabies prophylaxis is expensive and therefore not readily available in many endemic regions. Nervous tissue vaccine has been in use for more than 100yrs. These vaccines have now been superseded in purity, potency, immunogenicity and safety. The efficacy and immunogenicity of inactivated tissue culture rabies vaccine, produced in Ethiopia was evaluated. Twelve experimental dogs from local breed were duly conditioned during a quarantine period and assigned to two groups randomly. Animals in group I (cases) were vaccinated subcutaneously with 1 ml of our experimental vaccine. Dogs in group II served as non-vaccinated controls. The immune response of each dog was monitored for 90 days. On the day 90 after final sampling, all dogs were challenged in the masseter muscle with a rabies street virus of canine origin. To evaluate the titer of the rabies virus neutralizing antibodies (VNA), sera were analyzed by Fluorescent Antibody Virus Neutralization (FAVN) Test. Geometric Mean Titers (GMT) to rabies virus was determined at days 7, 15, 21, 30, 60 and 90. Geometric mean titers were equal to 1.59, 1.73, 2.19, 3.58, 3.17 and 3.35 IU/ml respectively. All dogs showed VNA titers higher than the 0.5 IU/ml mandated WHO recommended threshold. All vaccinated dogs, survived the challenge. In contrast, 83.3% of dogs in the control (non-vaccinated group), developed rabies and died. This study indicated that cell culture-based anti-rabies developed inhouse, with no adjuvant is efficacious and immunogenic

Mapping and modelling the geographical distribution and environmental limits of podoconiosis in Ethiopia

BACKGROUND Ethiopia is assumed to have the highest burden of podoconiosis globally, but the geographical distribution and environmental limits and correlates are yet to be fully investigated. In this paper we use data from a nationwide survey to address these issues. METHODOLOGY Our analyses are based on data arising from the integrated mapping of podoconiosis and lymphatic filariasis (LF) conducted in 2013, supplemented by data from an earlier mapping of LF in western Ethiopia in 2008-2010. The integrated mapping used woreda (district) health offices' reports of podoconiosis and LF to guide selection of survey sites. A suite of environmental and climatic data and boosted regression tree (BRT) modelling was used to investigate environmental limits and predict the probability of podoconiosis occurrence. PRINCIPAL FINDINGS Data were available for 141,238 individuals from 1,442 communities in 775 districts from all nine regional states and two city administrations of Ethiopia. In 41.9% of surveyed districts no cases of podoconiosis were identified, with all districts in Affar, Dire Dawa, Somali and Gambella regional states lacking the disease. The disease was most common, with lymphoedema positivity rate exceeding 5%, in the central highlands of Ethiopia, in Amhara, Oromia and Southern Nations, Nationalities and Peoples regional states. BRT modelling indicated that the probability of podoconiosis occurrence increased with increasing altitude, precipitation and silt fraction of soil and decreased with population density and clay content. Based on the BRT model, we estimate that in 2010, 34.9 (95% confidence interval [CI]: 20.2-51.7) million people (i.e. 43.8%; 95% CI: 25.3-64.8% of Ethiopia's national population) lived in areas environmentally suitable for the occurrence of podoconiosis. CONCLUSIONS Podoconiosis is more widespread in Ethiopia than previously estimated, but occurs in distinct geographical regions that are tied to identifiable environmental factors. The resultant maps can be used to guide programme planning and implementation and estimate disease burden in Ethiopia. This work provides a framework with which the geographical limits of podoconiosis could be delineated at a continental scale

Countdown to 2015: Ethiopia's progress towards reduction in under-five mortality: 2014 country case study

On September 13, 2013 the Federal Ministry of Health (FMoH) of Ethiopia and UNICEF announced that Ethiopia has successfully reduced the under-five mortality rate by two thirds between 1990 and 2012, which is the target for achieving Millennium Development Goal-4. In 1990, the under-five mortality rate in Ethiopia was one of the highest in the world at 205/1,000 live births. However, by 2012, this rate had declined to 68/1,000 live births with an average annual rate of decline of 5.0%. This exceeded the 4.3% annual rate of decline needed to reach MDG4 and was significantly higher than the decline rates observed in many sub-Saharan African countries and even other low and middle-income countries. In an effort to understand the story behind Ethiopia’s remarkable achievement of MDG-4, EPHI has conducted this in-depth Case Study which is supported by Countdown to 2015. The findings are believed to generate valuable lessons and guidance for other low-income countries in their quest for accelerating health improvements and reducing child deaths

Adherence to Tuberculosis Therapy among Patients Receiving Home-Based Directly Observed Treatment: Evidence from the United Republic of Tanzania.

\ud \ud Non-adherence to tuberculosis (TB) treatment is the leading contributor to the selection of drug-resistant strains of Mycobacterium tuberculosis and subsequent treatment failure. Tanzania introduced a TB Patient Centred Treatment (PCT) approach which gives new TB patients the choice between home-based treatment supervised by a treatment supporter of their own choice, and health facility-based treatment observed by a medical professional. The aim of this study was to assess the extent and determinants of adherence to anti-TB therapy in patients opting for home-based treatment under the novel PCT approach. In this cross-sectional study, the primary outcome was the percentage of patients adherent to TB therapy as detected by the presence of isoniazid in urine (IsoScreen assay). The primary analysis followed a non-inferiority approach in which adherence could not be lower than 75%. Logistic regression was used to examine the influence of potentially predictive factors. A total of 651 new TB patients were included. Of these, 645 (99.1%) provided urine for testing and 617 patients (95.7%; 90%CI 94.3-96.9) showed a positive result. This result was statistically non-inferior to the postulated adherence level of 75% (p<0.001). Adherence to TB therapy under home-based Directly Observed Treatment can be ensured in programmatic settings. A reliable supply of medication and the careful selection of treatment supporters, who preferably live very close to the patient, are crucial success factors. Finally, we recommend a cohort study to assess the rate of adherence throughout the full course of TB treatment

Mortality and Disability-adjusted Life-years (DALYs) for common neglected tropical Diseases in Ethiopia, 1990 to 2015: evidence from the Global Burden of Disease Study 2015

Introduction: Neglected tropical diseases (NTDs) are important public health problems in Ethiopia. In 2013, the Federal Ministry of Health (FMOH) has launched a national NTD master plan to eliminate major NTDs of public health importance by 2020. Benchmarking the current status of NTDs in the country is important to monitor and evaluate the progress in the implementation of interventions and their impacts. Therefore, this study aims to assess the trends of mortality and Disability-adjusted Life-Years (DALY) for the priority NTDs over the last 25 years. Methods: We used the Global Burden of Disease (GBD) 2015 estimates for this study. The GBD 2015 data source for cause of death and DALY estimation included verbal autopsy (VA), Demographic and Health Surveys (DHS), malaria indicator surveys (MICS) and other disease specific surveys, Ministry of Health reports submitted to United Nations (UN) agencies and published scientific articles. Cause of Death Ensemble modeling (CODEm) and/or natural history models were used to estimate malaria and NTDs mortality rates. DALY were estimated as the sum of Years of Life Lost (YLL) due to premature mortality and Years Lived with Disability (YLD). Results: All NTDs caused an estimated of 6,293 deaths (95% uncertainty interval (UI): 3699 – 10,080) in 1990 and 3,593 deaths (95% UI: 2051 – 6178) in 2015, a 70% reduction over the 25 years. Age-standardised mortality rates due to schistosomiasis, STH and leshmaniasis have declined by 91.3%, 73.5% and 21.6% respectively between 1990 to 2015. The number of DALYs due to all NTDs has declined from 814.4 thousand (95% UI: 548 thousand–1.2 million) in 1990 to 579.5 thousand (95%UI: 309.4 thousand – 1.3 million) in 2015. Age-standardised DALY rates due to all NTDs declined by 30.4%, from 17.6 per 1000(95%UI: 12.5-26.5) in 1990 to 12.2 per 1000(95%UI: 6.5 – 27.4) in 2015. Age-standardised DALY rate for trachoma declined from 92.7 per 100,000(95% UI: 63.2 – 128.4) in 1990 to 41.2 per 100,000(95%UI: 27.4 – 59.2) in 2015, a 55.6% reduction between 1990 and 2015. Age-standardised DALY rates for onchocerciasis, schistosomiasis and lymphiatic filariasis decreased by 66.2%, 29.4% and 12.5% respectively between 1990 and 2015. DALY rate for ascariasis fell by 56.8% over the past 25 years. Conclusions: Ethiopia has made a remarkable progress in reducing the DALY rates for most of the NTDs over the last 25 years. The rapid scale of interventions and broader system strengthening may have a lasting impact on achieving the 2020 goal of elimination of most of NTDs. Ethiopia should strengthen the coverage of integrated interventions of NTD through proper coordination with other health programs and sectors and community participation to eliminate NTDs by 2020

Spatial distribution of podoconiosis in relation to environmental factors in Ethiopia: a historical review

BACKGROUND An up-to-date and reliable map of podoconiosis is needed to design geographically targeted and cost-effective intervention in Ethiopia. Identifying the ecological correlates of the distribution of podoconiosis is the first step for distribution and risk maps. The objective of this study was to investigate the spatial distribution and ecological correlates of podoconiosis using historical and contemporary survey data. METHODS Data on the observed prevalence of podoconiosis were abstracted from published and unpublished literature into a standardized database, according to strict inclusion and exclusion criteria. In total, 10 studies conducted between 1969 and 2012 were included, and data were available for 401,674 individuals older than 15 years of age from 229 locations. A range of high resolution environmental factors were investigated to determine their association with podoconiosis prevalence, using logistic regression. RESULTS The prevalence of podoconiosis in Ethiopia was estimated at 3.4% (95% CI 3.3%-3.4%) with marked regional variation. We identified significant associations between mean annual Land Surface Temperature (LST), mean annual precipitation, topography of the land and fine soil texture and high prevalence of podoconiosis. The derived maps indicate both widespread occurrence of podoconiosis and a marked variability in prevalence of podoconiosis, with prevalence typically highest at altitudes >1500 m above sea level (masl), with >1500 mm annual rainfall and mean annual LST of 19-21°C. No (or very little) podoconiosis occurred at altitudes 24°C. CONCLUSION Podoconiosis remains a public health problem in Ethiopia over considerable areas of the country, but exhibits marked geographical variation associated in part with key environmental factors. This is work in progress and the results presented here will be refined in future work

Planar 17O NMR study of Pr_yY_{1-y}Ba_2Cu_3O_{6+x}

We report the planar ^{17}O NMR shift in Pr substituted YBa_{2}Cu_{3}O_{6+x}, which at x=1 exhibits a characteristic pseudogap temperature dependence, confirming that Pr reduces the concentration of mobile holes in the CuO_{2} planes. Our estimate of the rate of this counterdoping effect, obtained by comparison with the shift in pure samples with reduced oxygen content, is found insufficient to explain the observed reduction of T_c. From the temperature dependent magnetic broadening of the ^{17}O NMR we conclude that the Pr moment and the local magnetic defect induced in the CuO_2 planes produce a long range spin polarization in the planes, which is likely associated with the extra reduction of T_c. We find a qualitatively different behaviour in the oxygen depleted Pr_yY_{1-y}Ba_2Cu_3O_{6.6}, i.e. the suppression of T$_c$ is nearly the same, but the magnetic broadening of the ^{17}O NMR appears weaker. This difference may signal a weaker coupling of the Pr to the planes in the underdoped compound, which might be linked with the larger Pr to CuO_2 plane distance, and correspondingly weaker hybridization.Comment: 8 pages, 9 figures, accepted in Phys Rev