Physical conditions of the interstellar medium in star-forming galaxies at z~1.5

We present results from Subaru/FMOS near-infrared (NIR) spectroscopy of 118 star-forming galaxies at $z\sim1.5$ in the Subaru Deep Field. These galaxies are selected as [OII]$\lambda$3727 emitters at $z\approx$ 1.47 and 1.62 from narrow-band imaging. We detect H$\alpha$ emission line in 115 galaxies, [OIII]$\lambda$5007 emission line in 45 galaxies, and H$\beta$, [NII]$\lambda$6584, and [SII]$\lambda\lambda$6716,6731 in 13, 16, and 6 galaxies, respectively. Including the [OII] emission line, we use the six strong nebular emission lines in the individual and composite rest-frame optical spectra to investigate physical conditions of the interstellar medium in star-forming galaxies at $z\sim$1.5. We find a tight correlation between H$\alpha$ and [OII], which suggests that [OII] can be a good star formation rate (SFR) indicator for galaxies at $z\sim1.5$. The line ratios of H$\alpha$/[OII] are consistent with those of local galaxies. We also find that [OII] emitters have strong [OIII] emission lines. The [OIII]/[OII] ratios are larger than normal star-forming galaxies in the local Universe, suggesting a higher ionization parameter. Less massive galaxies have larger [OIII]/[OII] ratios. With evidence that the electron density is consistent with local galaxies, the high ionization of galaxies at high redshifts may be attributed to a harder radiation field by a young stellar population and/or an increase in the number of ionizing photons from each massive star.Comment: Fixed a minor issue with LaTeX table numberin

APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage

<p>Abstract</p> <p>Background</p> <p>The spindle assembly checkpoint (SAC) inhibits anaphase progression in the presence of insufficient kinetochore-microtubule attachments, but cells can eventually override mitotic arrest by a process known as mitotic slippage or adaptation. This is a problem for cancer chemotherapy using microtubule poisons.</p> <p>Results</p> <p>Here we describe mitotic slippage in yeast <it>bub2Δ </it>mutant cells that are defective in the repression of precocious telophase onset (mitotic exit). Precocious activation of anaphase promoting complex/cyclosome (APC/C)-Cdh1 caused mitotic slippage in the presence of nocodazole, while the SAC was still active. APC/C-Cdh1, but not APC/C-Cdc20, triggered anaphase progression (securin degradation, separase-mediated cohesin cleavage, sister-chromatid separation and chromosome missegregation), in addition to telophase onset (mitotic exit), during mitotic slippage. This demonstrates that an inhibitory system not only of APC/C-Cdc20 but also of APC/C-Cdh1 is critical for accurate chromosome segregation in the presence of insufficient kinetochore-microtubule attachments.</p> <p>Conclusions</p> <p>The sequential activation of APC/C-Cdc20 to APC/C-Cdh1 during mitosis is central to accurate mitosis. Precocious activation of APC/C-Cdh1 in metaphase (pre-anaphase) causes mitotic slippage in SAC-activated cells. For the prevention of mitotic slippage, concomitant inhibition of APC/C-Cdh1 may be effective for tumor therapy with mitotic spindle poisons in humans.</p

A New Constraint on the Lyα\alpha Fraction of UV Very Bright Galaxies at Redshift 7

We study the extent to which very bright (-23.0 < MUV < -21.75) Lyman-break selected galaxies at redshifts z~7 display detectable Lya emission. To explore this issue, we have obtained follow-up optical spectroscopy of 9 z~7 galaxies from a parent sample of 24 z~7 galaxy candidates selected from the 1.65 sq.deg COSMOS-UltraVISTA and SXDS-UDS survey fields using the latest near-infrared public survey data, and new ultra-deep Subaru z'-band imaging (which we also present and describe in this paper). Our spectroscopy has yielded only one possible detection of Lya at z=7.168 with a rest-frame equivalent width EW_0 = 3.7 (+1.7/-1.1) Angstrom. The relative weakness of this line, combined with our failure to detect Lya emission from the other spectroscopic targets allows us to place a new upper limit on the prevalence of strong Lya emission at these redshifts. For conservative calculation and to facilitate comparison with previous studies at lower redshifts, we derive a 1-sigma upper limit on the fraction of UV bright galaxies at z~7 that display EW_0 > 50 Angstrom, which we estimate to be < 0.23. This result may indicate a weak trend where the fraction of strong Lya emitters ceases to rise, and possibly falls between z~6 and z~7. Our results also leave open the possibility that strong Lya may still be more prevalent in the brightest galaxies in the reionization era than their fainter counterparts. A larger spectroscopic sample of galaxies is required to derive a more reliable constraint on the neutral hydrogen fraction at z~7 based on the Lya fraction in the bright galaxies.Comment: 20 pages, 7 figures, accepted for publication in Ap

Different cortical metabolic activation by visual stimuli possibly due to different time courses of hearing loss in patients with GJB2 and SLC26A4 mutations

Conclusion. We have demonstrated differences in cortical activation with language-related visual stimuli in patients who were profoundly deafened due to genetic mutations in GJB2 and SLC26A4. The differences in cortical processing patterns between these two cases may have been influenced by the differing clinical courses and pathogenesis of hearing loss due to genetic mutations. Our results suggest the importance of hearing during early childhood for the development of a normal cortical language network. Objectives. To investigate the cortical activation with language-related visual stimuli in patients who were profoundly deafened due to genetic mutations in GJB2 and SLC26A4. Methods: The cortical activity of two adult patients with known genetic mutations (GJB2, SLC26A4) was evaluated with fluorodeoxyglucose-positron emission tomography (FDG-PET) with a visual language task and compared with that of normal-hearing controls. Results: A patient with a GJB2 mutation showed activation in the right auditory association area [BA21, BA22], and the left auditory association area [BA42] even with visual language task; in contrast, a patient with an SLC26A4 mutation showed no significant activation in the corresponding area.ArticleACTA OTO-LARYNGOLOGICA. 131(11):1232-1236 (2011)journal articl

Gastric peroxisome proliferator activator receptor-γ expression and cytoprotective actions of its ligands against ischemia-reperfusion injury in rats

The beneficial effects by peroxisome proliferator-activated receptor-γ (PPAR-γ) on gastric injury induced by ischemia-reperfusion have been confirmed, however, the precise mechanism of its cytoprotection is not elucidated thoroughly. The aim of the present study was to determine the gastric localization of PPAR-γ expression in the rat gastric mucosa, and to clarify the mechanism of its cytoprotective properties. The gastric expression of PPAR-γ was confirmed by RT-PCR and western blot, and localized on gastric epithelial cells. The protective effect of PPAR-γ ligands, pioglitazone or 15-deoxy-Δ12,14-prostaglandin J2, on gastric ischemia-reperfusion injury was reversed by the co-administration with PPAR-γ antagonist. The gastric expression of tumor necrosis factor-α and cytokine-induced neutrophil chemoattractant-1 increased significantly in rats treated ischemia-reperfusion, and these increases were significantly inhibited by treatment with pioglitazone. Among the 1,032 probes, 18 probes were up-regulated at least 1.5-fold, 17 were down-regulated at least 1.5-fold by pioglitazone. The network including calnexin, endoplasmic reticulum stress protein, heat shock proteins, and proteasome genes was induced by pioglitazone treatment. In conclusion, activation of gastric epithelial PPAR-γ receptor by its ligands may represent a novel therapeutic approach for gastric inflammation via up-regulation of heat shock proteins and endoplasmic reticulum-related proteins

Biosynthesis and Release of Methylarsenic Compounds During the Growth of Freshwater Algae

金沢大学工学部Arsenic transformations by freshwater algae have been studied under laboratory conditions. By the use of a new analytical method, we identified methylarsenic(III) species in the growth medium of green-alga Closterium aciculare incubated under axenic conditions. The arsenate concentration in the experimental medium began to decrease just after inoculation, and the levels of arsenite and methylarsenicals increased with the growth of C. aciculare. Initially, most of the arsenate was converted into arsenite, which peaked in concentration during the exponential phase. Methylarsenicals accumulated rapidly in the stationary phase. DMAA(V) production was enhanced when the ratio of phosphate to arsenate decreased in the culture medium. The levels of DMAA(V) increased continuously toward the end of the experiment. On the other hand, methylarsenic(III) species remained relatively steady during the stationary phase. Methylarsenic(III) species accounted for 0-35% of methylarsenicals. These results suggest that arsenite and methylarsenicals (containing methylarsenic(III) species) are supplied by phytoplankton, and serve as evidence of the origin of methylarsenic(III) species in natural waters. © 2001 Elsevier Science Ltd

Expression of Superoxide Dismutase in Basal Cell Carcinoma

There have been no studies of the expression of superoxide dismutase(SOD) at the mRNA and protein level in skin cancers. Northern blot analysis and enzyme-linked immunosorbent assay (ELISA) were performed in order to analyze the expression of Cu, Zn-SOD and Mn-SOD in basal cell carcinomas (BCC) and normal skin (NS). The expression of Mn-SOD mRNA and protein was significantly higher in BCC than in NS. The expression of Cu,Zn-SOD, however, was high in BCC at the mRNA level, but not at the protein level. These results suggest that an increase in the expression of Mn-SOD relates to the development of BCCs

Magnifying Endoscopy with Blue Laser Imaging Improves the Microstructure Visualization in Early Gastric Cancer: Comparison of Magnifying Endoscopy with Narrow-Band Imaging

Backgrounds. Magnifying endoscopy with blue laser imaging (ME-BLI) for diagnosis of early gastric cancer (EGC) is as effective as magnifying endoscopy with narrow-band imaging (ME-NBI). However, there are different EGCs in microstructure visualization between ME-BLI and ME-NBI. This study aimed to clarify the pathological features of the EGCs, in which microstructure visualization was different between ME-NBI and ME-BLI. Methods. EGCs were classified into groups A (irregular microsurface pattern (MSP) in ME-BLI and absent MSP in ME-NBI), B (irregular MSP in two modalities), or C (absent MSP in two modalities), according to the vessel plus surface classification. We compared the pathological features of EGCs between the three groups. Results. 17, four, and five lesions could be evaluated in detail in groups A, B and C, respectively. Well-differentiated adenocarcinomas with shallow crypts were more frequent in group A than in group B (58.8 and 0%, resp.). The mean crypt depth of group A was significantly shallower than that of group B (56 ± 20, 265 ± 64 μm, resp., P=0.0002). Conclusions. ME-BLI could better visualize the microstructures of the EGCs with shallow crypts compared with ME-NBI. Therefore, ME-BLI could enable a more accurate diagnosis of EGC with shallow crypts