HENRY CARTER STUART: VIRGINIA FARMER - POLITICIAN, 1855-1933

Henry Carter Stuart was a prominent farmer and wealthy businessman in Southwestern Virginia. He was a politician in the hard fought Redeem the Ninth Congressional election of 1910. And, he was elected, unopposed in both the primary and general elections, to the governorship of Virginia in 1913. Although he reached the highest political office in the State, Virginia history gives Stuart no more than a mere mention. It was because of this lack of recognition that I selected to study Henry Carter Stuart. My purpose was to determine if he had been unfairly accorded the position of relative unimportance, or if Stuart, by virtue of his own action, failed to qualify himself for a place in Virginia history. Stuart was not the type of person to permit people to know about him or his private and business affairs. His descendants believe that before he died, he destroyed his private letters and papers. Therefore, it was necessary to compile this study through research of his associates, by interviews with is descendants and other people who knew him in the 1920\u27s and 1930\u27s, and through study of the political conditions during the time that Stuart was active in Virginia politics. The man who has received so little recognition of his political record evidentally preferred to leave no record at all. Therefore, I submit this study of Henry Carter Stuart: Virginia farmer, politician and incidentally, Governor

#CHIMoney: Financial interactions, digital cash, capital exchange and mobile money

Interactions around money and financial services are a critical part of our lives on and off-line. New technologies and new ways of interacting with these technologies are of huge interest; they enable new business models and ways of making sense of this most important aspect of our everyday lives. At the same time, money is an essential element in HCI research and design. This workshop is intended to bring together researchers and practitioners involved in the design and use of systems that combine digital and new media with monetary and financial interactions to build on an understanding of these technologies and their impacts on users' behaviors. The workshop will focus on social, technical, and economic aspects around everyday user interactions with money and emerging financial technologies and systems

Investigating the Effect of Energy Substrates and LPS-activation on the In Vitro Energy Metabolism of BV-2, RAW264.7 and VM-M3 Cells

Thesis advisor: Thomas N. SeyfriedTwo major metabolic phenomena observed in cancer cells include the Warburg effect and Crabtree effect. The Crabtree effect is the in vitro inhibition of respiration by glucose. The influence of glucose on the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of tumorigenic RAW264.7 and VM-M3 macrophage cells, as well as non-tumorigenic BV-2 microglia cells, was studied using the Seahorse XF96 extracellular flux analyzer. RAW264.7, VM-M3, and BV-2 cells incubated in glucose medium displayed a significantly lower OCR and higher ECAR compared to cells incubated in no glucose medium. Furthermore, when glucose medium was added to the RAW264.7 and BV-2 cells in real-time using the Seahorse XF96 injection ports, a rapid decrease in OCR and increase and ECAR was observed. Therefore, RAW264.7, VM-M3, and BV-2 cells display a robust Crabtree effect in vitro, as assessed by OCR and ECAR. Additionally, it is important to consider the Crabtree effect when studying in vitro energy metabolism of all cell and tissue types. It was also found that the elimination of the Crabtree effect through glucose deprivation resulted in dynamic cardiolipin (CL) fatty acid changes in VM-M3 cells. VM-M3 cells incubated in 10 mM glucose medium for four hours displayed a short-chain, saturated (immature) CL fatty acid composition, while VM-M3 cells incubated in no glucose media for four hours displayed long-chain, unsaturated (mature) CL fatty acid composition. Cardiolipin (CL) is a phospholipid highly enriched in the inner mitochondrial membrane. Mature, long-chain, unsaturated CL molecular species are involved in maintaining mitochondrial function and membrane integrity. Overall, these data suggest that CL fatty acid composition may function as a structural component of the Crabtree effect in vitro. The Warburg effect, or aerobic glycolysis, is the observation that tumor cells consume less oxygen and more glucose than normal, untransformed cells in the presence of oxygen. It has been shown that immune cells display a Warburg effect upon activation by changing their core metabolism from oxidative phosphorylation to glycolysis. In this study, it was observed that both RAW264.7 macrophage cells and BV-2 microglia cells display a significantly lower OCR and higher ECAR following LPS-activation. However, this observation is dependent on the concentration of LPS. Therefore, these data suggest that both RAW264.7 and BV-2 cells display a LPS concentration-dependent change in metabolism from oxidative phosphorylation to glycolysis upon LPS-activation in vitro. The in vitro lipid profiles that resulted from the Crabtree effect and the LPS-activated Warburg effect were also studied in the RAW264.7 cell line. The lipids phosphatidylserine (PS) and cardiolipin (CL) displayed the most robust changes in the RAW264.7 cells. Both PS and CL have been shown to be associated with cellular respiration.Thesis (MS) — Boston College, 2016.Submitted to: Boston College. Graduate School of Arts and Sciences.Discipline: Biology

Dissecting pathways to thrombocytopenia in a mouse model of visceral leishmaniasis

Visceral leishmaniasis is an important yet neglected parasitic disease caused by infection with Leishmania donovani or L infantum. Disease manifestations include fever, weight loss, hepatosplenomegaly, immune dysregulation, and extensive hematological complications. Thrombocytopenia is a dominant hematological feature seen in both humans and experimental models, but the mechanisms behind this infection-driven thrombocytopenia remain poorly understood. Using a murine model of experimental visceral leishmaniasis (EVL), we demonstrated a progressive decrease in platelets from day 14 after infection, culminating in severe thrombocytopenia by day 28. Plasma thrombopoietin (TPO) levels were reduced in infected mice, at least in part because of the alterations in the liver microenvironment associated with granulomatous inflammation. Bone marrow (BM) megakaryocyte cytoplasmic maturation was significantly reduced. In addition to a production deficit, we identified significant increases in platelet clearance. L donovani–infected splenectomized mice were protected from thrombocytopenia compared with sham operated infected mice and had a greater response to exogenous TPO. Furthermore, infection led to higher levels of platelet opsonization and desialylation, both associated with platelet clearance in spleen and liver, respectively. Critically, these changes could be reversed rapidly by drug treatment to reduce parasite load or by administration of TPO agonists. In summary, our findings demonstrate that the mechanisms underpinning thrombocytopenia in EVL are multifactorial and reversible, with no obvious residual damage to the BM microenvironment

100 years of chemistry at Rhodes University

The history of Grahamstown is well documented and two books deal with the history of Rhodes University.1,2 Although the Chemistry Department was one of the founding departments, coverage in the official histories is minimal and sometimes inaccurate or misleading. The Rhodes University Centenary is an appropriate time to look back on some of the achievements of the department and some of its graduates over the past 100 years

Factors Associated With Choice in Health Insurance Decisions

Following an earlier exploratory survey of health insurance choice and decision-making, and an extensive literature review, a survey was carried out using as a population the employees of two large organisations. The objectives were (a) to extend the earlier survey in content and to a larger population; and (b) to gather information on reasons for choice and information sought and used in making decisions. The decision to insure and level of cover chosen were related to expected characteristics such as age, income and marital status together with history of medical expenses. History of hospital costs played a lesser role in choice of level of cover. Neither dependents nor health status nor expectations of costs appeared to be important and, although the proportion of insured was high, the population and their families were very healthy. In general, information sought and used was meagre and there is considerable support for concluding that decisions are not based on considered analysis of risks, costs and available options. Rather it would appear that decisions are related to general notions of risk aversion, the influence of media coverage of health care matters and aggressive advertising by the health funds. For those who did not insure, ideological factors - private enterprise medicine and rights to health care - were important

Ten principles relevant to health research among Indigenous Australian populations

Writing in the Journal about Indigenous health in 2011, Sir Michael Marmot suggested that the challenge was to conduct research, and to ultimately apply findings from that research, to enable Indigenous Australians to lead more flourishing lives that they would have reason to value.1 As committed Indigenous health researchers in Australia, we reflect Marmot’s ideal — to provide the answers to key questions relating to health that might enable Indigenous Australians to live the lives that they would choose to live.As a group, we have over 120 collective years’ experience in Indigenous health research. Over this time, particularly in recent years as ethical guidelines have come into play, there have been many examples of research done well. However, as the pool of researchers is constantly replenished, we hold persisting concerns that some emerging researchers may not be well versed in the principles of best practice regarding research among Indigenous Australian populations. Implementing any research methodology among Indigenous Australian groups will work best when the following 10 principles are met. These principles are reflected in the many documents related to working and researching with Indigenous Australians; for example, the National Health and Medical Research Council (NHMRC) ethical guidelines for research among Aboriginal and Torres Strait Islander people.2 In this article, we set out these principles in one short, accessible document