Evaluation of Germination, Emergence and Physiological Properties of Sugar Beet Cultivars Under Salinity

DergiPark: 947001trkjnatThis study aimed to determine a useful selection criterion for salt tolerance during the early development stage of sugar beet. Four sugar beet cultivars (Orthega, Valentina, FD Shoot, and Mohican) were exposed to NaCl stresses (Control, 5, 10, and 15 dS m-1), and morphological and physiological characteristics were investigated. Germination percentage, mean germination time (MGT), seedling length, and seedling fresh weight (SFW) in germination test; emergence percentage, mean emergence time (MET), root length, shoot length, plant fresh weight, relative chlorophyll content (Chl), relative water content (RWC) and electrolyte leakage of the plants grown in pod experiment were measured. The results showed that the maximum germination at control was recorded in FD Shoot, but it gave the lowest germination at 15 dS m-1. In the pod experiment, the highest emergence rate was detected in Orthega and Mohican at all levels of NaCl. Increased salinity delayed MET and led to reduction in shoot length, root length, and RWC of sugar beet cultivars. Relative Chl content and electrolyte leakage enhanced from 32.7 SPAD and 21.6% in control to 38.5 SPAD and 35.6% in 10 dS m-1, respectively. In general, there were significant differences among sugar beet cultivars, and they could keep the salinity up to 5 dS m-1 in terms of the investigated traits. It was concluded that relative Chl content and electrolyte leakage should be used a promising clue for selection of tolerant or sensitive sugar beet cultivars for salinity.Bu çalışmada, erken gelişim döneminde şeker pancarının tuza toleransı için faydalı bir seçim kriteri belirlemek amaçlanmıştır. NaCl stresine (Kontrol, 5, 10 ve 15 dS m-1) maruz bırakılan dört şeker pancarı çeşidinde (Orthega, Valentina, FD Shoot ve Mohican) morfolojik ve fizyolojik özellikler incelenmiştir. Çimlenme testinde; çimlenme yüzdesi, ortalama çimlenme süresi, fide uzunluğu ve fide yaş ağırlığı, çıkış testinde; çıkış yüzdesi, ortalama çıkış süresi, kök uzunluğu, sürgün uzunluğu, bitki yaş ağırlığı, bağıl su içeriği, bağıl klorofil içeriği ve elektrolit sızıntısı ölçülmüştür. Sonuçlar, FD Shoot çeşidinde en yüksek çimlenmenin kontrol, en düşük çimlenmenin ise 15 dS m-1 seviyesinde kaydedildiğini göstermiştir. Çıkış testindeki tüm NaCl seviyelerinde en yüksek çıkış yüzdesi Orthega ve Mohican çeşitlerinde tespit edilmiştir. Artan NaCl seviyeleri ile şeker pancarı çeşitlerinde ortalama çıkış süresi gecikmiş ve sürgün uzunluğu, kök uzunluğu ve bağıl su içeriği azalmıştır. Bağıl klorofil içeriği ve elektrolit sızıntısı, kontrol ve 10 dS m-1 seviyelerinde sırasıyla 32,7 SPAD ve %21,6; 38,5 SPAD ve %35,6 olarak belirlenmiştir. Genel olarak, şeker pancarı çeşitleri arasında önemli farklılıklar bulunmuş ve incelenen özellikler açısından çeşitler 5 dS m-1'e kadar olan tuzluluğa tolerans göstermişlerdir. Bağıl klorofil içeriği ve elektrolit sızıntısının, tuzluluğa toleranslı veya hassas şeker pancarı çeşitlerinin seçiminde umut verici bir ipucu olarak kullanılması gerektiği sonucuna varılmıştır.

A Case of Vogt-Koyanagi-Harada Disease Associated With Polycystic Ovary Syndrome

We report a female patient diagnosed as Vogt-Koyanagi-Harada (VKH) and polycystic ovary syndrome (PCOS). She has diagnosed as VKH with diminished vision, bilateral serous retinal detachment, the signs of fundus fluorescein angiography and the findings of optical coherence tomography. The patient was referred to the gynecology clinic for her complaints as weight gain, hirsutismus and amenorrhea. She has also been diagnosed with PCOS. With oral steroid treatment, visual acuity has improved and the detachments have resolved within a month. VKH disease may be associated with polycystic ovary syndrome. The two conditions may have a common autoimmune pathogenesis

Autotetraploid plant production in endemic Onobrychis elata with colchicine treatments

This study aimed to induce autotetraploidy in endemic Onobrychis elata plants by colchicine treatment of seeds or seedlings. Colchicine was applied to O. elata directly on germinated seeds, pre-germinated seeds (root length of 3-8 mm), and apical regions (using cotton) under in vivo conditions. Out of a total of 1,210 colchicine-treated seeds that were evaluated, only 203 survived. There was an inverse relationship between the number of surviving plants and colchicine concentration and exposure time. The highest percentage of tetraploidy in surviving plants (50%) was obtained by applying 0.2% colchicine for 6 hours to pre-germinated seeds. No significant tetraploidy was achieved by colchicine application to seedlings. Flow cytometry observations indicated that DNA content varied between 0.99 and 1.06 pg in diploid plants (controls), while DNA content varied between 2.22 and 2.48 pg in tetraploid plants. It was concluded that tetraploid plants were induced successfully only in seedlings obtained from pre-germinated seeds, with their ploidy level confirmed via flow cytometry analysis

Recent Advances in Health Biotechnology During Pandemic

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in 2019, cut the epoch that will make profound fluctuates in the history of the world in social, economic, and scientific fields. Urgent needs in public health have brought with them innovative approaches, including diagnosis, prevention, and treatment. To exceed the coronavirus disease 2019 (COVID-19) pandemic, various scientific authorities in the world have procreated advances in real time polymerase chain reaction (RT-PCR) based diagnostic tests, rapid diagnostic kits, the development of vaccines for immunization, and the purposing pharmaceuticals for treatment. Diagnosis, treatment, and immunization approaches put for- ward by scientific communities are cross-fed from the accrued knowledge of multidisciplinary sciences in health biotechnology. So much so that the pandemic, urgently prioritized in the world, is not only viral infections but also has been the pulsion in the development of novel approaches in many fields such as diagnosis, treatment, translational medicine, virology, mi- crobiology, immunology, functional nano- and bio-materials, bioinformatics, molecular biol- ogy, genetics, tissue engineering, biomedical devices, and artificial intelligence technologies. In this review, the effects of the COVID-19 pandemic on the development of various scientific areas of health biotechnology are discussed

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

A New Approach to Determine Time and Temperature Combination for Electrical Conductivity Test in Sorghum

This study was conducted to determine a suitable time and temperature combination for the electrical conductivity test to be used in sorghum seeds. Fifty seeds known initial seed moisture content and weight of fresh and dead seeds (105°C for 6h) of seven sorghum cultivars were used as material. The electrical conductivities of soaking water were measured using an EC meter in 20, 25 and 30°C for 4, 8, 12 and 24 h using 50 mL deionized water. The experimental design was three factor factorial (7 × 3 × 4) arranged in a completely randomized design; with four replications and 50 seeds per replicate. The results showed that increased time and temperature caused a remarkable increase in EC values of all of the cultivars. Temperature significantly affected the electrical conductivity values and the best results were obtained at 25°C. The cultivars with the lower germination percentage gave the higher electrical conductivity value. Dead seeds always gave higher electrical conductivity at 25°C for all periods. It was concluded that the temperature of 25°C and 24 h was the optimum combination for the electrical conductivity test in sorghum