The diverse world of career guidance

This book is the product of an EU funded project involving parterns from The Czech Republic, Denmark and the United Kingdom. The book contains personal reflections of career guidance provision and activities in which theory and practice are united through the eyes of experienced practitioners from a range of guidance settings. This book is aimed at both established and new guidance practitionersEU Erasmus

High-contrast differentiation resolution 3D imaging of rodent brain by X-ray computed microtomography

The biomedically focused brain research is largely performed on laboratory mice considering a high homology between the human and mouse genomes. A brain has an intricate and highly complex geometrical structure that is hard to display and analyse using only 2D methods. Applying some fast and efficient methods of brain visualization in 3D will be crucial for the neurobiology in the future. A post-mortem analysis of experimental animals' brains usually involves techniques such as magnetic resonance and computed tomography. These techniques are employed to visualize abnormalities in the brains' morphology or reparation processes. The X-ray computed microtomography (micro CT) plays an important role in the 3D imaging of internal structures of a large variety of soft and hard tissues. This non-destructive technique is applied in biological studies because the lab-based CT devices enable to obtain a several-micrometer resolution. However, this technique is always used along with some visualization methods, which are based on the tissue staining and thus differentiate soft tissues in biological samples. Here, a modified chemical contrasting protocol of tissues for a micro CT usage is introduced as the best tool for ex vivo 3D imaging of a post-mortem mouse brain. This way, the micro CT provides a high spatial resolution of the brain microscopic anatomy together with a high tissue differentiation contrast enabling to identify more anatomical details in the brain. As the micro CT allows a consequent reconstruction of the brain structures into a coherent 3D model, some small morphological changes can be given into context of their mutual spatial relationships

Zavedení obchodního controllingu ve vybrané firmě

Import 10/08/2007Prezenční152 - Katedra podnikohospodářsk

Structure and properties of AB21, a novel Agaricus bisporus protein with structural relation to bacterial pore-forming toxins

We report the characterization of the dimeric protein AB21 from Agaricus bisporus, one of the most commonly and widely consumed mushrooms in the world. The protein shares no significant sequence similarity with any protein of known function, and it is the first characterized member of its protein family. The coding sequence of the ab21 gene was determined and the protein was expressed in E. coli in a recombinant form. We demonstrated a high thermal and pH stability of AB21 and proved the weak affinity of the protein to divalent ions of some transition metals (nickel, zinc, cadmium, and cobalt). The reported crystallographic structure exhibits an interesting rod‐like helical bundle fold with structural similarity to bacterial toxins of the ClyA superfamily. By immunostaining, we demonstrated an abundance of AB21 in the fruiting bodies of A. bisporus

XRF and magnetic susceptibility records from sediments of Suzdalevo Lake (Tunguska region, Russia) – cores SUZ1 and SUZ3

Here, we present X-ray fluorescence (SM30 magnetic susceptibility meter, ZHinstruments Inc.) and normalized mass magnetic susceptibility (VANTA VMR with a Silicon Drift Detector, Olympus) datasets of two sediment cores (0.42 and 0.46 m long) from a shallow lake (Suzdalevo Lake, 1.8 ha, max. depth of 2.3 m) located near the epicenter of the Tunguska Event explosion in 1908 CE (60°39′29.05″N, 102°3′3.36″E). The datasets include primary data presented in an article (Kavková et al. 2022) that is focused on formation process of the study site (potential impact lake) and Tunguska Event-related environmental disturbance. Both cores were retrieved using a Kajak gravity corer (sampling tube diameter of 5.8 cm and length of 50 cm) in May 2019

Biological indicator records (diatoms, chrysophyte cysts, freshwater invertebrates) from sediments of Suzdalevo Lake (Tunguska region, Russia) – sediment core SUZ1

Here, we present diatom (Bacillariophyceae), chrysophyte (Chrysophyceae), and zoological indicator (Chironomidae, other aquatic insect, planktonic crustaceans, Bryozoa, Rhabdocoela) taxonomic datasets from a sediment core (core ID: SUZ1, length of 0.42 m) record from a shallow lake (Suzdalevo Lake, 1.8 ha, max. depth of 2.3 m) located near the epicenter of the Tunguska Event explosion in 1908 CE (60°39′29.05″N, 102°3′3.36″E). The datasets include primary data presented in an article (Kavková et al. 2022) that is focused on formation process of the study site (potential impact lake) and Tunguska Event-related environmental disturbance. The core was retrieved using a Kajak gravity corer (sampling tube diameter of 5.8 cm and length of 50 cm) in May 2019

A Practitioner's Guide to Uncharted Waters of Career Counselling, a Critical Reflection Perspective

This is a practical book intended for career practitioners working with young people in schools and other institutions providing career guidance and counselling. The aim is to offer practitioners support so that they can feel empowered in their roles as career counsellors, and are able to take care of themselves and gain new ideas for their practice. The book is one output of an Erasmus funded project which invovled partners from the UK, Denmark, Greece, Spain and the Czech Republic.The research is sponsored by the Erasmus + programme of the European Unio