La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

10 décembre 18691869/12/10 (A4,N1331)

Additional file 3: Table S3. of A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala

LSP-PCR and LRPS sample analyses. (DOC 43 kb

Bactericidal activity in sputum according to treatment arm as assessed by colony counts (left) and time to positivity in automated liquid culture (MGIT TTP, right).

<p>Lines indicate prediction and shading 90% confidence interval (CI) as determined by mixed effects model repeated measures analysis, using day as a categorical variable. The vertical axis of the right hand figure is inverted to facilitate visual comparison with CFU findings. At 14 days, the 90% CI of all treatments excluded zero.</p

Distributions of minimal inhibitory concentrations (MICs) to sutezolid (PNU-100480) pre and post treatment (rows and columns, respectively), according to dosing schedule.

<p>Values in each cell indicate numbers of patients. Cells shaded red are those in which MIC values increased, whereas they decreased in those shaded blue. No change was apparent in MIC values of the metabolite (PNU-101603, not shown).</p

Geometric mean pharmacokinetic parameters in patients of sutezolid and its major metabolite following dosing for 14 Days.

<p>CV = coefficient of variation.</p

Plasma concentrations of sutezolid (pink) and its major metabolite (yellow) at steady state (day 13–14) in patients treated with sutezolid 600 mg BID (left) or 1200 mg QD (right).

<p>Solid lines indicate medians; shading indicates 90% CI. Lower and upper dotted lines indicate median pre-treatment MIC values for parent and metabolite, respectively.</p

Treatment-emergent ALT increases in sutezolid-treated subjects.

<p>Treatment-emergent ALT increases in sutezolid-treated subjects.</p

La fonction résidentielle dans un centre-ville en mutation : le cas de la ville de Chicoutimi,

La présente recherche s'inscrit dans le cadre du Programme de Maîtrise en Etude des Sociétés Régionales et constitue une exigence essentielle à l'obtention du diplôme de fin d'études. Bien que très spécialisée et davantage tournée vers les disciplines de l'urbanisme et de la géographie urbaine, celle-ci s'inscrit dans le cadre de l'analyse du phénomène régional. La ville de Chicoutimi n'est certes pas l'image-type des villes de la région du Saguenay-Lac-St-Jean mais elle en constitue cependant l'une des composantes importantes qu'il importe de connaître. Ainsi, dans l'analyse du phénomène d'évolution des centres-villes en milieu périphérique tel la région du Saguenay-Lac-St-Jean, il importe de connaître en premier chef, ce qui se passe à 1'intérieur des villes les plus populeuses. En fournissant pour la ville de Chicoutimi différentes données sur les caractéristiques socio-économiques des résidents et sur les conditions générales d'habitabilité dans la zone centrale, nous pensons apporter une contribution supplémentaire au domaine de recherche particulier que constitue la géographie urbaine

Performance Comparison of Three Rapid Tests for the Diagnosis of Drug-Resistant Tuberculosis

<div><p>Background</p><p>The aim of this study was to compare the performance of several recently developed assays for the detection of multi- and extensively drug-resistant tuberculosis (M/XDR-TB) in a large, multinational field trial.</p><p>Methods</p><p>Samples from 1,128 M/XDR-TB suspects were examined by Line Probe Assay (LPA), Pyrosequencing (PSQ), and Microscopic Observation of Drug Susceptibility (MODS) and compared to the BACTEC MGIT960 reference standard to detect M/XDR-TB directly from patient sputum samples collected at TB clinics in India, Moldova, and South Africa.</p><p>Results</p><p>Specificity for all three assays was excellent: 97–100% for isoniazid (INH), rifampin (RIF), moxifloxacin (MOX) and ofloxacin (OFX) and 99–100% for amikacin (AMK), capreomycin (CAP) and kanamycin (KAN) resistance. Sensitivities were lower, but still very good: 94–100% for INH, RIF, MOX and OFX, and 84–90% for AMK and CAP, but only 48–62% for KAN. In terms of agreement, statistically significant differences were only found for detection of RIF (MODS outperformed PSQ) and KAN (MODS outperformed LPA and PSQ) resistance. Mean time-to-result was 1.1 days for LPA and PSQ, 14.3 days for MODS, and 24.7 days for MGIT.</p><p>Conclusions</p><p>All three rapid assays evaluated provide clinicians with timely detection of resistance to the drugs tested; with molecular results available one day following laboratory receipt of samples. In particular, the very high specificity seen for detection of drug resistance means that clinicians can use the results of these rapid tests to avoid the use of toxic drugs to which the infecting organism is resistant and develop treatment regiments that have a higher likelihood of yielding a successful outcome.</p></div

Flowchart of patient enrollment, clinical sample processing for Line Probe Assay (LPA), Microscopic Observation of Drug Susceptibility (MODS), and Pyrosequencing (PSQ) for seven anti-TB drugs: isoniazid (INH), rifampin (RIF), moxifloxacin (MOX), ofloxacin (OFX), amikacin (AMK), kanamycin (KAN), and capreomycin (CAP).

<p>Includes one null PSQ sample result (denoted as 'a'). R: Resistant, S: Susceptible, and I: Indeterminate.</p