Study of Antimicrobial Activity & Structural Elucidation of Purified Compound from Potential Solvent Extract of Bruguiera cylindrica [L]

B.Cylindrica is used as a mangrove plant in folk medicine to cure various diseases and disorders which include tumors, blood clotting, infections, fevers, pains etc., The Present study was to isolation, purification and characterization of antibacterial compound from the leaves along with phytochemical analysis. Healthy leaves of B. cylindrica were collected from natural habitat, washed, air dried and ground into powder. The solvents, such as chloroform, ethyl acetate, ethanol, methanol, and butanol were used to extract the powdered leaves. Crude extracts were tested for phytochemical constituents like carbohydrates, glycosides, proteins, steroids, triterpenoids flavonoids, alkaloids, tannins, saponins and phenols. Antimicrobial activities for crude extracts were tested against bacteria and fungi using standard well diffusion agar plate method. The highest antibacterial activity was identified in ethyl acetate extract, which was further purified using column chromatography. Activity-guided ethyl acetate fraction contains potential compound was used for structure elucidation using LC-MS, FTIR and NMR studies. Mass spectrometry of purified compound showed major peak at 6.901 minutes with a molecular weight of 256.32 g/mol, suggesting that the compound belongs to carboxylic acid group with a molecular formula CHN2O3 when compared to LC-MS standard from the NIST library. The Pure compound from Bruguiera cylindrica showed the stretch at 2561 indicated the presence of (O-H) hydroxyl group, 3341 N- H group, a stretch at 1709 indicated the presence of C=O group. The data obtained showed the presence of nitrogen containing amine, amide and carboxylic acid functional groups 7-(dimethylamino)-3ethyl-8-oxo-5-thia-1-azabicyclo(4.2.0)-oct-2-ene-2-carboxylic acid with a molecular formula CHN2O3. Based on 1H NMR spectrum and database of NIST, purified fraction was identified as 7-(dimethylamino)-3ethyl-8-oxo-5-thia-1-azabicyclo(4.2.0)-oct-2-ene-2-carboxylic acid. The results show that B cylindrica contains significant phytochemical and antibacterial activity against tested bacteria and fungi that helps to scientifically understand potentiality of tribal medicine

Acylation and alkylation of 1, 3-dimethoxybenzene in polyphosphoric acid

Products of the reaction of 3-halo- and 3-ethoxypropionic acids with 1,3-dimethoxybenzene (4) in polyphosphoric acid have been shown to be 2',4'-dimethoxy-3-(2,4-dimethoxyphenyl)-propiophenone (6) and 1,5-bis[3-(2,4-dimethoxyphenyl)-3-keto-propyl]-2,4-dimethoxybenzene (15) and these have been prepared by unambiguous syntheses. 2',4'-Dimethoxy-3-ethoxypropiophenone (3a) and 2',3,4'- trimethoxypropiophenone (3d) have been synthesized by the reaction of 3-chloro-2',4'-dimethoxypropiophenone (3c) with ethanol and methanol respectively

Enhanced Two-Photon Absorption in a Hollow-Core Photonic Bandgap Fiber

We show that two-photon absorption (TPA) in Rubidium atoms can be greatly enhanced by the use of a hollow-core photonic bandgap fiber. We investigate off-resonant, degenerate Doppler-free TPA on the 5S1/2 - 5D5/2 transition and observe 1% absorption of a pump beam with a total power of only 1 mW in the fiber. These results are verified by measuring the amount of emitted blue fluorescence and are consistent with the theoretical predictions which indicate that transit time effects play an important role in determining the two-photon absorption cross-section in a confined geometry.Comment: 5 pages, 6 figure

Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle

Hyaluronan binding protein 1 (HABP1) is a negatively charged multifunctional mammalian protein with a unique structural fold. Despite the fact that HABP1 possesses mitochondrial localization signal, it has also been localized to other cellular compartments. Using indirect immunofluorescence, we examined the sub-cellular localization of HABP1 and its dynamics during mitosis. We wanted to determine whether it distributes in any distinctive manner after mitotic nuclear envelope disassembly or is dispersed randomly throughout the cell. Our results reveal the golgi localization of HABP1 and demonstrate its complete dispersion throughout the cell during mitosis. This distinctive distribution pattern of HABP1 during mitosis resembles its ligand hyaluronan, suggesting that in concert with each other the two molecules play critical roles in this dynamic process

Progress in Tropical Cyclone Predictability and Present Status in the North Indian Ocean Region

Tropical cyclone (TC) is an important research area since it has a significant impact on human life, properties and environment. The researchers all over the world have been studying fundamental and advanced processes to better understand and thereby predict the genesis and evolution of TCs. This review chapter provides a brief overview on TC climatology, their basic characteristics, movement and intensification, research on structure analysis and prediction of these fascinating storms, with primary emphasis to North Indian Ocean (NIO). The role of ocean and atmosphere in determining the genesis and intensification of TCs is discussed. This chapter reviews the past and current research activities including inter-annual and intra-seasonal changes in TCs, current status of TC research using numerical weather prediction, gaps identified and relevant measures taken by the meteorological and government agencies in this direction, along with future directions in order to improve the understanding and predictability over the NIO region

Protein Engineering Of Bt Genes cry1Ab And cry1Ba For The Development Of Chimeric Genes cryAbabba, cryBabaab And cryAbbaab Via Domain Swapping

Bacillus thuringiensis is renowned for its production of insecticidal cry proteins, widely utilized in crop protection to combat insects. However, the risk of insect resistance emerges due to the relatively loose binding of toxins to target sites on larvae's midgut brush boundary membranes. This resistance primarily stems from modifications in binding sites within midgut cells. To address potential threats, the discovery of new Cry proteins is imperative as insects continually evolve resistance against existing ones. Combining Cry toxins with diverse binding sites in larval midguts is proposed as an effective strategy to delay the onset of resistance. In this study, three chimeric B. thuringiensis proteins—CryAbAbBa, CryBaBaAb, and CryAbBaAb—were engineered via domain swapping, utilizing crystal proteins CrylAb and CrylB. Structural validation was conducted, confirming their integrity through Ramachandran Plots. The chimeric proteins can be used as additional resources in crop improvement programmes

Faster Algorithms for the Geometric Transportation Problem

Let R, B be a set of n points in R^d, for constant d, where the points of R have integer supplies, points of B have integer demands, and the sum of supply is equal to the sum of demand. Let d(.,.) be a suitable distance function such as the L_p distance. The transportation problem asks to find a map tau : R x B --> N such that sum_{b in B}tau(r,b) = supply(r), sum_{r in R}tau(r,b) = demand(b), and sum_{r in R, b in B} tau(r,b) d(r,b) is minimized. We present three new results for the transportation problem when d(.,.) is any L_p metric: * For any constant epsilon > 0, an O(n^{1+epsilon}) expected time randomized algorithm that returns a transportation map with expected cost O(log^2(1/epsilon)) times the optimal cost. * For any epsilon > 0, a (1+epsilon)-approximation in O(n^{3/2}epsilon^{-d}polylog(U)polylog(n)) time, where U is the maximum supply or demand of any point. * An exact strongly polynomial O(n^2 polylog n) time algorithm, for d = 2

Magnetic resonance studies on the interaction of metal-ion and nucleotide ligands with brain hexokinase

Our previous studies have shown that one manganous ion binds tightly to bovine brain hexokinase, with a Kd =25 ± 4 μM. The characteristic proton relaxation rate (PRR) enhancement of this binary complex (εb) 3 .5 at 9 MHz and 23 °C [ Jarori, G. K. Kasturi, S. R., and Kenkare, U. W. (1981) Arch. Biochem. Biophys. 211,258 - 2681. On the basis of PRR enhancement patterns, observed on the addition of nucleotides ATP and ADP to this E . Mn binary complex, we now show the formation of a nucleotide-bridge ternary complex, enzyme . nucleotide . Mn. Addition of glucose 6-phosphate to enzyme . ATP . Mn, results in a competitive displacement of ATP Mn from the enzyme. However, a quaternary complex E · ADP· Mn· Glc-6-P appears to be formed when both the products are present. β, γ-Bidentate Cr(II1)ATP has been used to elucidate the role of direct binding of Mn(I1) in catalysis, and the stoichiometry of metal-ion interaction with the enzyme in the presence of nucleotide. Bidentate Cr(II1)ATP serves as a substrate for brain hexokinase without any additional requirement for a divalent cation. However, electron-spin resonance studies on the binding of Mn(I1) to the enzyme in the presence of Cr(I1I)ATP suggest that, in the presence of nucleotide, two metal ions interact with hexokinase, one binding directly to the enzyme and the second interacting via the nucleotide bridge. It is this latter one which participates in catalysis. Experiments carried out with hexokinase spin-labeled with 3-(2-iodo-acetamido)-2,2,5,5-tetramethyl-lpyrrolidinyloxyl clearly showed that the direct-binding Mn site on the enzyme is distinctly located from its ATP Mn binding site