P-Element Homing Is Facilitated by engrailed Polycomb-Group Response Elements in Drosophila melanogaster

P-element vectors are commonly used to make transgenic Drosophila and generally insert in the genome in a nonselective manner. However, when specific fragments of regulatory DNA from a few Drosophila genes are incorporated into P-transposons, they cause the vectors to be inserted near the gene from which the DNA fragment was derived. This is called P-element homing. We mapped the minimal DNA fragment that could mediate homing to the engrailed/invected region of the genome. A 1.6 kb fragment of engrailed regulatory DNA that contains two Polycomb-group response elements (PREs) was sufficient for homing. We made flies that contain a 1.5kb deletion of engrailed DNA (enΔ1.5) in situ, including the PREs and the majority of the fragment that mediates homing. Remarkably, homing still occurs onto the enΔ1. 5 chromosome. In addition to homing to en, P[en] inserts near Polycomb group target genes at an increased frequency compared to P[EPgy2], a vector used to generate 18,214 insertions for the Drosophila gene disruption project. We suggest that homing is mediated by interactions between multiple proteins bound to the homing fragment and proteins bound to multiple areas of the engrailed/invected chromatin domain. Chromatin structure may also play a role in homing

Fitting Isochrones to Open Cluster photometric data: A new global optimization tool

We present a new technique to fit color-magnitude diagrams of open clusters based on the Cross-Entropy global optimization algorithm. The method uses theoretical isochrones available in the literature and maximizes a weighted likelihood function based on distances measured in the color-magnitude space. The weights are obtained through a non parametric technique that takes into account the star distance to the observed center of the cluster, observed magnitude uncertainties, the stellar density profile of the cluster among others. The parameters determined simultaneously are distance, reddening, age and metallicity. The method takes binary fraction into account and uses a Monte-Carlo approach to obtain uncertainties on the determined parameters for the cluster by running the fitting algorithm many times with a re-sampled data set through a bootstrapping procedure. We present results for 9 well studied open clusters, based on 15 distinct data sets, and show that the results are consistent with previous studies. The method is shown to be reliable and free of the subjectivity of most previous visual isochrone fitting techniques.Comment: 19 pages, 25 figures, accepted for publication in Astronomy&Astrophysic

Variable stars in the field of the old open cluster Melotte 66

We report the results of photometric monitoring of the Melotte 66 field in BVI filters. Ten variables were identified with nine being new discoveries. The sample includes eight eclipsing binaries of which four are W UMa type stars, one star is a candidate blue straggler. All four contact binaries are likely members of the cluster based on their estimated distances. Ten blue stars with U-B<-0.3 were detected inside a 14.8 x 22.8 arcmin^2 field centred on the cluster. Time series photometry for 7 of them showed no evidence for any variability. The brightest object in the sample of blue stars is a promising candidate for a hot subdwarf belonging to the cluster. We show that the anomalously wide main sequence of the cluster, reported in some earlier studies, results from a combination of two effects: variable reddening occuring across the cluster field and the presence of a rich population of binary stars in the cluster itself. The density profile of the cluster field is derived and the total number of member stars with 16<V<21 or 2.8<M_{V}<7.8 is estimated conservatively at about 1100.Comment: 8 pages, 12 figures, accepted to MNRAS - 29 June 200

Comet 162P/Siding Spring: A Surprisingly Large Nucleus

We present an analysis of thermal emission from comet 162P/Siding Spring (P/2004 TU12) measured during its discovery apparition in 2004 December. The comet showed no dust coma at this time, so we have sampled emission from the comet's nucleus. Observations using the Mid-Infrared Spectrometer and Imager (MIRSI) were performed at NASA's Infrared Telescope Facility, where the peak of the comet's spectral energy distribution was observed between 8 and 25 microns. In combination with the three near-IR spectra presented by Campins et al. (2006, Astron. J. 132, 1346) that show the Wien-law tail of the thermal emission, the data provide powerful constraints on surface properties of the nucleus. We find that the nucleus's effective radius is 6.0+/-0.8 km. This is one of the largest radii known among Jupiter-family comets, which is unusual considering that the comet was discovered only recently. Its geometric albedo is 0.059+/-0.023 in the H band, 0.037+/-0.014 in the R band, and 0.034+/-0.013 in the V band. We also find that the nucleus of 162P has little IR beaming, and this implies that the nucleus has low thermal inertia. Including all near-IR spectra yields a beaming parameter of 1.01+/-0.20. This result is in agreement with others showing that cometary nuclei have low thermal inertia and little IR beaming. If confirmed for many nuclei, the interpretation of radiometry may not be as problematic as feared.Comment: 17 pages, 4 figures, appearing in The Astronomical Journal, September 2006 issu

Inhibition of Y1 receptor signaling improves islet transplant outcome

Failure to secrete sufficient quantities of insulin is a pathological feature of type-1 and type-2 diabetes, and also reduces the success of islet cell transplantation. Here we demonstrate that Y1 receptor signaling inhibits insulin release in β-cells, and show that this can be pharmacologically exploited to boost insulin secretion. Transplanting islets with Y1 receptor deficiency accelerates the normalization of hyperglycemia in chemically induced diabetic recipient mice, which can also be achieved by short-term pharmacological blockade of Y1 receptors in transplanted mouse and human islets. Furthermore, treatment of non-obese diabetic mice with a Y1 receptor antagonist delays the onset of diabetes. Mechanistically, Y1 receptor signaling inhibits the production of cAMP in islets, which via CREB mediated pathways results in the down-regulation of several key enzymes in glycolysis and ATP production. Thus, manipulating Y1 receptor signaling in β-cells offers a unique therapeutic opportunity for correcting insulin deficiency as it occurs in the pathological state of type-1 diabetes as well as during islet transplantation.Islet transplantation is considered one of the potential treatments for T1DM but limited islet survival and their impaired function pose limitations to this approach. Here Loh et al. show that the Y1 receptor is expressed in β- cells and inhibition of its signalling, both genetic and pharmacological, improves mouse and human islet function.info:eu-repo/semantics/publishe

A contemporaneous infrared flash from a long gamma-ray burst: an echo from the central engine

The explosion that results in a cosmic gamma-ray burst (GRB) is thought to produce emission from two physical processes -- the activity of the central engine gives rise to the high-energy emission of the burst through internal shocking and the subsequent interaction of the flow with the external environment produces long-wavelength afterglow. While afterglow observations continue to refine our understanding of GRB progenitors and relativistic shocks, gamma-ray observations alone have not yielded a clear picture of the origin of the prompt emission nor details of the central engine. Only one concurrent visible-light transient has been found and was associated with emission from an external shock. Here we report the discovery of infrared (IR) emission contemporaneous with a GRB, beginning 7.2 minutes after the onset of GRB 041219a. Our robotic telescope acquired 21 images during the active phase of the burst, yielding the earliest multi-colour observations of any long-wavelength emission associated with a GRB. Analysis of an initial IR pulse suggests an origin consistent with internal shocks. This opens a new possibility to study the central engine of GRBs with ground-based observations at long wavelengths.Comment: Accepted to Nature on March 1, 2005. 9 pages, 4 figures, nature12.cls and nature1.cls files included. This paper is under press embargo until print publicatio

Innovations and advances in instrumentation at the W. M. Keck Observatory

Since the start of operations in 1993, the twin 10 meter W. M. Keck Observatory telescopes have continued to maximize their scientific impact and to produce transformative discoveries that keep the observing community on the frontiers of astronomical research. Upgraded capabilities and new instrumentation are provided though collaborative partnerships with Caltech and UC instrument development teams. The observatory adapts and responds to the observers’ evolving needs as defined in the observatory’s strategic plan, periodically refreshed in collaboration with the science community. This paper summarizes the performance of recently commissioned infrastructure projects, technology upgrades, and new additions to the suite of instrumentation at the observatory. We will also provide a status of projects currently in the design or development phase, and since we need to keep our eye on the future, we mention projects in exploratory phases that originate from our strategic plan. Recently commissioned projects include telescope control system upgrades, OSIRIS spectrometer and imager upgrades, and deployments of the Keck Cosmic Web Imager (KCWI), the Near-Infrared Echellette Spectrometer (NIRES), and the Keck I Deployable Tertiary Mirror (KIDM3). Under development are upgrades to the NIRSPEC instrument and adaptive optics (AO) system. Major instrumentation in design phases include the Keck Cosmic Reionization Mapper and the Keck Planet Finder. Future instrumentation studies and proposals underway include a Ground Layer Adaptive Optics system, NIRC2 upgrades, the energy sensitive instrument KRAKENS, an integral field spectrograph LIGER, and a laser tomography AO upgrade. Last, we briefly discuss recovering MOSFIRE and its return to science operations