Experimental Intradermal Granuloma Formation**From the Department of Pathology, Beth Israel Hospital, Harvard Medical School, and the Division of Tumor Immunology, Children's Cancer Research Foundation, Boston, Massachusetts. (Reprint requeats to Dr. E. J. Kasdon, Department of Pathology, Beth Israel Hospital, 330 Brookline Avenue, Boston, Mass. 02215.)

Experimental induction of an intradermal granulomatous hypersensitivity reaction in the primarily immunized guinea pig was studied by means of tight microscopy during the course of the immune response. Animals were sensitized to dinitrophenylated human serum albumin (DNP10-HSA), and challenged with this antigen covalently linked to Sepharose 2B beads by the cyanogen bromide reaction. At appropriate intervals, the dermal changes produced by this complex antigen were compared with those produced by DNP10-HSA or soluble antigen. Sepharose 2B beads atone, and DNP10-HSA plus Sepharose 2B in the same animal. The complex antigen produced lesions characteristic of granulomatous hypersensitivity 5 and 11 days beyond the time of the skin test. The others showed the characteristic responses of hypersensitivity, foreign-body reaction, and a mixture of delayed-hypersensitivity and foreign-body reaction, but the intensity of the reactions had diminished by 5 days and had disappeared after 11 days. These results indicate that a dermal granulomatous hypersensitivity reaction may be differentiated from a foreign-body reaction. They also suggest that locally retained antigen interacts with sensitized lymphocytes to contribute to granuloma formation

Network space in ambulatory care facilities

Thesis. 1979. M.Arch.--Massachusetts Institute of Technology. Dept. of Architecture.MICROFICHE COPY AVAILABLE IN ARCHIVES AND ROTCH.by William L. Kasdon.M.Arch

Percutaneous radiofrequency lesions adjacent to the dorsal root ganglion alleviate spasticity and pain in children with cerebral palsy: pilot study in 17 patients

BACKGROUND: Cerebral palsy (CP) may cause severe spasticity, requiring neurosurgical procedures. The most common neurosurgical procedures are continuous infusion of intrathecal baclofen and selective dorsal rhizotomy. Both are invasive and complex procedures. We hypothesized that a percutaneous radiofrequency lesion of the dorsal root ganglion (RF-DRG) could be a simple and safe alternative treatment. We undertook a pilot study to test this hypothesis. METHODS: We performed an RF-DRG procedure in 17 consecutive CP patients with severe hip flexor/adductor spasms accompanied by pain or care-giving difficulties. Six children were systematically evaluated at baseline, and 1 month and 6 months after treatment by means of the Modified Ashworth Scale (MAS), Gross Motor Function Measure (GMFM) and a self-made caregiver's questionnaire. Eleven subsequent children were evaluated using a Visual Analogue Scale (VAS) for spasticity, pain and ease of care. RESULTS: A total of 19 RF-DRG treatments were performed in 17 patients. We found a small improvement in muscle tone measured by MAS, but no effect on the GMFM scale. Despite this, the caregivers of these six treated children unanimously stated that the quality of life of their children had indeed improved after the RF-DRG. In the subsequent 11 children we found improvements in all VAS scores, in a range comparable to the conventional treatment options. CONCLUSION: RF-DRG is a promising new treatment option for severe spasticity in CP patients, and its definitive effectiveness remains to be defined in a randomised controlled trial

Child Centered Play-Therapy with a Child Diagnosed with Kabuki Syndrome

Kabuki Syndrome (KS) is a rare genetic disorder first diagnosed in 1981 (Matsumoto & Niikawa, 2003). It\u27s clinical presentation and treatment is unknown by most clinicians the mental health fields. Children with KS present with unique facial characteristics, mental retardation, health problems and socio-emotional delays that are often mistaken for other diagnostic problems. Literature detailing the psychological and psychosocial features of this disorder is scant, and psychotherapeutic approaches have not been described. In this article we present a brief review of Kabuki Syndrome, highlighting its signs and symptoms. Differential diagnoses are identified to aid the clinician in better understanding this unique and relatively unheard of syndrome. Finally, a client-centered play therapy and parent consultation approach is described that addresses the many child and family challenges that may accompany KS

The Effectiveness of in vitro Somatic Embryogenesis in Eliminating Fanleaf Virus and Leafroll Associated Viruses from Grapevines

Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries extracted from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV) and grapevine leafroll associated viruses (GLR) respectively. Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) as well as serological tests (ELISA). Results indicated that somatic embryogenesis derived from ovary tissue of infected grapevines is an effective technique to eliminate grapevine Ieafroll associated viruses from grapevines but the procedure was not successful in the elimination of GFL V from anther source material

The Effectiveness of in vitro Somatic Embryogenesis in Eliminating Fanleaf Virus and Leafroll Associated Viruses from Grapevines

Somatic embryos were successfully regenerated from callus tissue of anthers and ovaries extracted from inflorescences of grapevines infected with grapevine fanleaf virus (GFLV) and grapevine leafroll associated viruses (GLR) respectively. Production of pro-embryogenic masses (PEMS) was controlled by specific growth regulators and culture conditions. Somatic embryos (containing roots and cotyledons) and plantlets were subjected to immunosorbent electron microscopy (ISEM) as well as serological tests (ELISA). Results indicated that somatic embryogenesis derived from ovary tissue of infected grapevines is an effective technique to eliminate grapevine Ieafroll associated viruses from grapevines but the procedure was not successful in the elimination of GFL V from anther source material