64 research outputs found
6 Ma age of carving Westernmost Grand Canyon: Reconciling geologic data with combined AFT, (U-Th)/He, and He-4/He-3 thermochronologic data
Conflicting hypotheses about the timing of carving of the Grand Canyon involve either a 70 Ma (“old”) or <6 Ma (“young”) Grand Canyon. This paper evaluates the controversial westernmost segment of the Grand Canyon where the following lines of published evidence firmly favor a “young” Canyon. 1) North-derived Paleocene Hindu Fanglomerate was deposited across the present track of the westernmost Grand Canyon, which therefore was not present at ∼55 Ma. 2) The 19 Ma Separation Point basalt is stranded between high relief side canyons feeding the main stem of the Colorado River and was emplaced before these tributaries and the main canyon were incised. 3) Geomorphic constraints indicate that relief generation in tributaries and on plateaus adjacent to the westernmost Grand Canyon took place after 17 Ma. 4) The late Miocene–Pliocene Muddy Creek Formation constraint shows that no river carrying far-traveled materials exited at the mouth of the Grand Canyon until after 6 Ma.
Interpretations of previously-published low-temperature thermochronologic data conflict with these lines of evidence, but are reconciled in this paper via the integration of three methods of analyses on the same sample: apatite (U–Th)/He ages (AHe), 4He/3He thermochronometry (4He/3He), and apatite fission-track ages and lengths (AFT). HeFTy software was used to generate time–temperature (t–T) paths that predict all new and published 4He/3He, AHe, and AFT data to within assumed uncertainties. These t–T paths show cooling from ∼100 °C to 40–60 °C in the Laramide (70–50 Ma), long-term residence at 40–60 °C in the mid-Tertiary (50–10 Ma), and cooling to near-surface temperatures after 10 Ma, and thus support young incision of the westernmost Grand Canyon.
A subset of AHe data, when interpreted alone (i.e. without 4He/3He or AFT data), are better predicted by t–T paths that cool to surface temperatures during the Laramide, consistent with an “old” Grand Canyon. However, the combined AFT, AHe, and 4He/3He analysis of a key sample from Separation Canyon can only be reconciled by a “young” Canyon. Additional new AFT (5 samples) and AHe data (3 samples) in several locations along the canyon corridor also support a “young” Canyon. This inconsistency, which mimics the overall controversy of the age of the Grand Canyon, is reconciled here by optimizing cooling paths so they are most consistent with multiple thermochronometers from the same rocks. To do this, we adjusted model parameters and uncertainties to account for uncertainty in the rate of radiation damage annealing in these apatites during sedimentary burial and the resulting variations in He retentivity. In westernmost Grand Canyon, peak burial conditions (temperature and duration) during the Laramide were likely insufficient to fully anneal radiation damage that accumulated during prolonged, near-surface residence since the Proterozoic. We conclude that application of multiple thermochronometers from common rocks reconciles conflicting thermochronologic interpretations and the data presented here are best explained by a “young” westernmost Grand Canyon. Samples spread along the river corridor also suggest the possibility of variable mid-Tertiary thermal histories beneath north-retreating cliffs
Attenuation of Notch and Hedgehog Signaling Is Required for Fate Specification in the Spinal Cord
During the development of the spinal cord, proliferative neural progenitors differentiate into postmitotic neurons with distinct fates. How cells switch from progenitor states to differentiated fates is poorly understood. To address this question, we studied the differentiation of progenitors in the zebrafish spinal cord, focusing on the differentiation of Kolmer-Agduhr″ (KA″) interneurons from lateral floor plate (LFP) progenitors. In vivo cell tracking demonstrates that KA″ cells are generated from LFP progenitors by both symmetric and asymmetric cell divisions. A photoconvertible reporter of signaling history (PHRESH) reveals distinct temporal profiles of Hh response: LFP progenitors continuously respond to Hh, while KA″ cells lose Hh response upon differentiation. Hh signaling is required in LFP progenitors for KA″ fate specification, but prolonged Hh signaling interferes with KA″ differentiation. Notch signaling acts permissively to maintain LFP progenitor cells: activation of Notch signaling prevents differentiation, whereas inhibition of Notch signaling results in differentiation of ectopic KA″ cells. These results indicate that neural progenitors depend on Notch signaling to maintain Hh responsiveness and rely on Hh signaling to induce fate identity, whereas proper differentiation depends on the attenuation of both Notch and Hh signaling
Visualization of Gli Activity in Craniofacial Tissues of Hedgehog-Pathway Reporter Transgenic Zebrafish
The Hedgehog (Hh)-signaling pathway plays a crucial role in the development and maintenance of multiple vertebrate and invertebrate organ systems. Gli transcription factors are regulated by Hh-signaling and act as downstream effectors of the pathway to activate Hh-target genes. Understanding the requirements for Hh-signaling in organisms can be gained by assessing Gli activity in a spatial and temporal fashion.We have generated a Gli-dependent (Gli-d) transgenic line, Tg(Gli-d:mCherry), that allows for rapid and simple detection of Hh-responding cell populations in both live and fixed zebrafish. This transgenic line expresses a mCherry reporter under the control of a Gli responsive promoter, which can be followed by using fluorescent microscopy and in situ hybridization. Expression of the mCherry transgene reporter during embryogenesis and early larval development faithfully replicated known expression domains of Hh-signaling in zebrafish, and abrogating Hh-signaling in transgenic fish resulted in the suppression of reporter expression. Moreover, ectopic shh expression in Tg(Glid:mCherry) fish led to increased transgene production. Using this transgenic line we investigated the nature of Hh-pathway response during early craniofacial development and determined that the neural crest skeletal precursors do not directly respond to Hh-signaling prior to 48 hours post fertilization, suggesting that earlier requirements for pathway activation in this population of facial skeleton precursors are indirect.We have determined that early Hh-signaling requirements in craniofacial development are indirect. We further demonstrate the Tg(Gli-d:mCherry) fish are a highly useful tool for studying Hh-signaling dependent processes during embryogenesis and larval stages
Selenium isotope evidence for progressive oxidation of the Neoproterozoic biosphere
Neoproterozoic (1,000–542 Myr ago) Earth experienced profound environmental change, including ‘snowball’ glaciations, oxygenation and the appearance of animals. However, an integrated understanding of these events remains elusive, partly because proxies that track subtle oceanic or atmospheric redox trends are lacking. Here we utilize selenium (Se) isotopes as a tracer of Earth redox conditions. We find temporal trends towards lower δ82/76Se values in shales before and after all Neoproterozoic glaciations, which we interpret as incomplete reduction of Se oxyanions. Trends suggest that deep-ocean Se oxyanion concentrations increased because of progressive atmospheric and deep-ocean oxidation. Immediately after the Marinoan glaciation, higher δ82/76Se values superpose the general decline. This may indicate less oxic conditions with lower availability of oxyanions or increased bioproductivity along continental margins that captured heavy seawater δ82/76Se into buried organics. Overall, increased ocean oxidation and atmospheric O2 extended over at least 100 million years, setting the stage for early animal evolution
Linking Human Diseases to Animal Models Using Ontology-Based Phenotype Annotation
A novel method for quantifying the similarity between phenotypes by the use of ontologies can be used to search for candidate genes, pathway members, and human disease models on the basis of phenotypes alone
Breast and prostate cancer patients differ significantly in their serum Thymidine kinase 1 (TK1) specific activities compared with those hematological malignancies and blood donors: implications of using serum TK1 as a biomarker
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Looping P-T paths and high-T, low-P middle crustal metamorphism: Proterozoic evolution of the southwestern United States
Proterozoic rocks of the southwestern United States exhibit a variety of tectonometamorphic histories that can all be explained by a characteristic looping style of pressure-temperature (P-T) path. The path is fundamentally clockwise. However, because the rocks were cooled isobarically at 3 kbar after tectonism, the cooling path crosses the prograde path, leading to a geometry with a counterclockwise character. The absolute size of the loop in the P-T path varies across the region because of different amounts of overthickening (and resulting exhumation) during the contractional event, combined with variable amounts of heating from syntectonic plutons. This style of looping P-T path may be characteristic of high-T, low-P metamorphic terranes and middle crustal tectonism, particularly where syntectonic plutons contribute to elevated geotherms and polymetamorphism and where rocks become stabilized and reside for long periods at middle-crustal depths before being exhumed during a later tectonic event
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Heterogeneity of the middle crust: Implications for strength of continental lithosphere
Borehole logs from the northern Barbados accretionary prism show that the plate-boundary decollement initiates in a low-density radiolarian claystone. With continued thrusting, the decollement zone consolidates, but in a patchy manner. The logs calibrate a three-dimensional seismic reflection image of the decollement zone and indicate which portions are of low density and enriched in fluid, and which portions have consolidated. The seismic image demonstrates that an underconsolidated patch of the decollement zone connects to a fluid-rich conduit extending down the decollement surface. Fluid migration up this conduit probably supports the open pore structure in the underconsolidated patch
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Crustal segmentation, composite looping pressure-temperature paths, and magma-enhanced metamorphic field gradients: Upper Granite Gorge, Grand Canyon, USA
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