Analysis for prevalence and physical linkages amongst integrons, ISEcp1, ISCR1, Tn21 and Tn7 encountered in Escherichia coli strains from hospitalized and non-hospitalized patients in Kenya during a 19-year period (1992-2011)

Background: We determined the prevalence and evidence for physical linkage amongst integrons, insertion sequences, Tn21 and Tn7 transposons in a collection of 1327 E. coli obtained over a 19-year period from patients in Kenya. Results: The prevalence of class 1 integrons was 35%, class 2 integrons were detected in 3 isolates but no isolate contained a class 3 integron. Integron lacking the 3'-CS or those linked to sul3 gene or IS26 or those containing the ISCR1 were only detected in multidrug resistant (MDR) strains. The dfrAs were the most common cassettes and their prevalence was: -dfrA1( 28%), dfrA12( 20%), dfA17( 9%), dfrA7( 9%), and dfrA16( 5%). The aadA were the second most abundant cassettes and their prevalence was: -aadA1( 25%), aadA2( 21%), and aadA5( 14%). Other cassettes occurred in lower prevalence of below 5%. Prevalence of Tn21, ISEcp1, ISCR1 and IS26 was 22%, 10%, 15%, and 7% respectively. Majority of Tn21 containing integrons carried a complete set of transposition genes while class 2 integrons were borne on Tn7 transposon. The qnrA genes were detected in 34( 3%) isolates while 19( 1%) carried qnrB. All qnr genes were in MDR strains carrying integrons containing the ISCR1. Close to 88% of blaTEM-52 were linked to IS26 while = 80% of blaCTX-Ms and blaCMYs were linked to ISEcp1. Only a few studies have identified a blaCTX-M-9 containing an ISEcp1 element as reported in this study. Multiple genetic elements, especially those borne on incIl, incFII, and incL/M plasmids, and their associated resistance genes were transferrable en bloc to E. coli strain J53 in mating experiments. Conclusions: This is the first detailed study on the prevalence of selected elements implicated in evolution of resistance determinants in a large collection of clinical E. coli in Africa. Proliferation of such strains carrying multiple resistance elements is likely to compromise the use of affordable and available treatment options for majority of poor patients in Africa. There is therefore a need to monitor the spread of these highly resistant strains in developing countries through proper infection control and appropriate use of antimicrobials

The Relationship Between Student and Faculty Learning Style Congruency and Perceptions of the Classroom Environment in Colleges of Teacher Education

The purpose of this study was to determine the extent of congruence between teachers\u27 and undergraduate education majors\u27 learning styles in selected colleges of the Tennessee Association of Colleges for Teacher Education, and to determine if the style congruence was related to student perceptions of the classroom learning environment. A related purpose was to identify needed changes in classroom environments based on the characteristics of the actual and ideal classroom environments as perceived by students, characteristics of the actual classroom environment as perceived by their teachers, and characteristics of actual and ideal classroom environments as perceived by men and women students. A relationship of classroom environments was also examined. Kolb\u27s Learning Style Inventory and the Adult Classroom Environment Scale were administered to students and teachers in selected colleges for teacher education that were members of the Tennessee Association of Colleges for Teacher Education during the Fall, 1994. Data were analyzed using measures of central tendency and measures of dispersion, t-tests for dependent (correlated) means, t-tests for independent means, and Pearson Product Moment Correlations. Results indicated that the predominant learning style for both students and teachers was Accommodator. The students preferred Diverger as their second dominant learning style while the teachers preferred Assimilator. The teachers incorporated logical thinking, systematic thinking, and intellectual thinking in their learning behavior, while the students preferred to learn by viewing situations from different points of view and to observe without taking action. Matching students\u27 learning styles with those of teachers was not found to be related to the ratings of the classroom environment. Significant relationships were found to exist between all classroom dimensions except Task Orientation and Student Influence. Both teachers and students viewed Teacher Support as the most prevalent element of the actual classroom environment and Student Influence as the least noticeable element of the classroom environment. However, the teachers\u27 views for the actual classroom environment were higher than students\u27 views in all subscales except for Organization and Clarity

Prototype for tracking voluntary blood donors in enhancing emergency medical response: case Aga Khan Hospital

Thesis submitted in partial fulfillment of the requirements for the Degree of Master of Science in Information Technology (MSIT) at Strathmore UniversityThe World Health Organization recommends in its Global Database on Blood Safety that all activities related to blood collection, screening, processing, storage and distribution should be coordinated at the national level through effective organization and a national blood policy and system. The researcher developed a prototype that comprised of an android mobile application, and a front end application. The researcher used prototyping methodology to develop the application. The prototype developed in this research aims at making use of the existing telecommunication infrastructure while merging this with the blood bank’s information technology systems. The target population comprised of employees working within the Aga Khan University Hospital Blood Transfusion Center and voluntary non remunerable donors. Both quantitative and qualitative techniques were used to evaluate the information collected. The study concludes that it is difficult to get the domicile location of, voluntary donors. By having system that tracks voluntary donor movement, it is possible to enhance emergency medical response that requires blood transfusion. The diverse activities in the blood donation and distribution service can be streamlined as a result of data mining capabilities

Analysis of β-lactamase phenotypes and carriage of selected β-lactamase genes among Escherichia coli strains obtained from Kenyan patients during an 18-year period

Background: Although beta-lactam antibiotics are heavily used in many developing countries, the diversity of beta-lactamase genes (bla) is poorly understood. We screened for major beta-lactamase phenotypes and diversity of bla genes among 912 E. coli strains isolated from clinical samples obtained between 1992 and 2010 from hospitalized and non-hospitalized patients. Results: None of the isolates was resistant to carbapenems but 30% of all isolates were susceptible to cefepime, cephamycins and piperacillin-tazobactam. Narrow spectrum beta-lactamase (NSBL) phenotype was observed in 278 (30%) isolates that contained bla(TEM-1) (54%) or bla(SHV-1) (35%) or both (11%). Extended Spectrum beta-lactamase (ESBL) phenotype was detected in 247 (27%) isolates which carried bla(CTX-M-14) (29%), bla(CTX-M-15) (24%), bla(CTX-M-9) (2%), bla(CTX-M-8) (4%), bla(CTX-M-3) (11%), bla(CTX-M-1) (6%), bla(SHV-5) (3%), bla(SHV-12) (5%), and bla(TEM-52) (16%). Complex Mutant TEM-like (CMT) phenotype was detected in 220 (24%) isolates which carried bla(TEM-125) (29%), while bla(TEM-50), bla(TEM-78), bla(TEM-109), bla(TEM -152) and bla(TEM-158) were detected in lower frequencies of between 7% and 11%. Majority of isolates producing a combination of CTX-M-15+OXA-1+TEM-1 exhibited resistance phenotypes barely indistinguishable from those of CMT-producers. Although 73 (8%) isolates exhibited Inhibitor Resistant TEM-like (IRT) phenotype, bla(TEM-103) was the only true IRT-encoding gene identified in 18 (25%) of strains with this phenotype while the rest produced a combination of TEM-1 + OXA-1. The pAmpCs-like phenotype was observed in 94 (10%) isolates of which 77 (82%) carried bla(CMY-2) while 18% contained bla(CMY-1). Isolates from urine accounted for 53%, 53%, 74% and 72% of strains exhibiting complex phenotypes such as IRT, ESBL, CMT or pAmpC respectively. On the contrary, 55% isolates from stool exhibited the relatively more susceptible NSBL-like phenotype. All the phenotypes, and majority of the bla genes, were detected both in isolates from hospitalized and non-hospitalized patients but complex phenotypes were particularly common among strains obtained between 2000 and 2010 from urine of hospitalized patients. Conclusions: The phenotypes and diversity of bla genes in E. coli strains implicated in clinical infections in non-hospitalized and hospitalized patients in Kenya is worryingly high. In order to preserve the efficacy of beta-lactam antibiotics, culture and susceptibility data should guide therapy and surveillance studies for beta-lactamase-producers in developing countries should be launched

Income Diversification and Intermediation Efficiency: Evidence from Deposit Taking Sacco Societies in Kenya

Research on the relationship between diversification into nontraditional income streams and firm efficiency is scanty. The study seeks to fill the gap by evaluating the relationship between diversification into non interest income and intermediation efficiency of Deposit Taking Sacco Societies (DTSs) in Kenya using a two staged methodology. In the first stage, efficiency scores are generated using Data Envelopment Analysis (DEA), corrected for bias using bootstrapping and used as dependent variable in the fixed effect regression model estimated in the second stage. A balanced panel data of 103 DTSs for a period 2011-2014 was used in the study. The results showed that there exists an inverse relationship between the ratio of noninterest income to total assets and intermediation efficiency. This implied that diversification hurts efficiency. Keywords: Data Envelopment Analysis, Deposit Taking Sacco, Diversification, Intermediation Efficiency, Noninterest income

The contribution of charcoal production to rural livelihoods in a semi-arid area in Kenya

Forest incomes in the form of both timber and non-timber forest products are an important source of livelihood for many communities in Africa. A major forest resource is charcoal, which contributes to the livelihoods of millions across the region. While incomes from charcoal are used to meet a wide spectrum of needs within rural livelihoods, the role of charcoal production on livelihoods of small-scale producers is not well understood. Therefore, we provide an example on the importance of charcoal on livelihoods in an agropastoralist community in a semi-arid region in Kenya. Based on a household questionnaire targeting 150 charcoal and 150 non-charcoal makers, as well as focus group discussions, we assessed the determinants for participation in charcoal production and developed a household typology based on charcoal income dependence. We also determined the role of charcoal in income equalization and poverty reduction. Our study shows that charcoal contributes about 20% of the household income in the study area. Gender, land size and the number of food-scarce months are the key determinants of participation in charcoal production. Based on the poverty analysis, we conclude that even though charcoal income does not lift the producers out of poverty, it can mitigate the impacts of poverty by reducing the poverty gap and poverty severity. Based on our findings, we recommend a multipronged approach to address sustainable rural livelihoods including a more explicit acknowledgement of charcoal production as a source of rural income. We also recommend broadening of the local livelihood base and a more active management of the woodland to ensure the sustainability of the income

Rainfall and Temperature Trends and Variability in Arid and Semi-arid Lands of Kitui County, Kenya

A study was carried out to analyse rainfall and temperature trends and variability in selected agro-ecological zones in Kitui County. Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) rainfall dataset was used while temperature data was obtained from the Climatic Research Unit gridded Time Series (CRU TS) dataset. The results indicated that there was a non-significant decreasing trend (p<0.05) in average annual rainfall in all the four agro-ecological zones (p<0.05) for a 30-years period (1988-2018) in the study area. A decreasing trend in March-April-May (MAM) seasonal rainfall trend was reported in the arid and semi-arid agro-ecological zones while an increasing trend was recorded in the transitional and semi-humid zones. For the October-November-December (OND) seasonal rainfall, a non-significant decreasing trend was reported in all the four agro-ecological zones. Additionally, a higher annual rainfall variability was recorded in the drier (arid) and wetter (semi-humid) zones compared to that in semi-arid and transitional zones. Moreover, the study established that there was a spatial variation in both MAM and OND seasonal rainfall variability and that rainfall variability was higher in the OND seasonal rainfall than that of the MAM seasonal rainfall in all the agro-ecological zones. With reference to temperature trends, a statistically significant increasing trend in annual and OND seasonal average maximum and minimum temperatures was reported in all the four agro-ecological zones. Further, the study noted a non-significant increasing trend in maximum and minimum temperatures for the MAM season in all the agro-ecological zones. In regard to temperature variability, the study deduced that there was low temperature variability compared to rainfall variability in all the four agro-ecological zones. The study recommends that location-specific rainfall and temperature analysis should guide planning and implementation of adaptation strategies for effective response to climate variability. Keywords: Agro-ecological Zones, Mann-Kendall Test, Coefficient of Variation, CHIRPS, CRU TS DOI: 10.7176/JEES/12-12-05 Publication date: December 31st 202

Nairobi Special Housing Fund

Over the next 15 years, over Kshs. 89.09 billion will be paid by slum-dwellers to informal service-providers for low-quality and high-cost services. This situation is replicated in almost all informal settlements within the city of Nairobi. Developing affordable shelter for the urban poor, and thereby realizing the Constitutional right to housing, will require new forms of pro-poor shelter finance and supportive policies at the urban level. But most of Nairobi’s residential construction has targeted wealthy households, leaving insufficient supply of low-income housing where the needs are most urgent. This paper argues for a County-level affordable housing fund

Application of Remote Sensing in Mapping Hydrothermal Alteration Zones and Geological Structures as Areas of Economic Mineralization in Mwitika-Makongo Area, SE Kenya

Remote sensing investigations combined with Geographical investigation systems (GIS) have become more important for the study of geological mineralization and structural geology like lineaments. Automatic extraction of lineaments from satellite imagery help in giving an overview of the tectonic events in the study area. The main objective of the study was to map hydrothermally altered rocks and geological structures that may be associated with mineral deposits in Mwitika-Makongo area. The study involves the use of Landsat- 8/OLI image. The satellite images were classified using ENVI 5.3 and ArcGIS 10.5. PCI geomatics 2016 was used for extraction of lineaments while Rose diagram was generated using Rockworks 16. Different remote sensing techniques, such as colour composite, band ratio and principal component analysis were applied to identify geological units and features related to economic mineralization. Colour composite band combination (5, 6, 7) showed hydrothermally altered geological units as blue. Band ratio combination (4/2, 6/7, 6/5) showed the areas that were hydrothermally altered as blue. Lineament mapping was done using PCA, with the first three PCs having the highest percentage of Eigenvalues. A lineament density map showed higher values in Makongo hill followed by Kalima Kathei hill. The structural trend from the rose diagram is in the NE direction. The results of image analysis and lineament extraction show that the economic minerals like iron ore are located in areas of hydrothermal alterations where intrusive rocks like Pyroxenite were found. Keywords: Colour composite, Geographical investigation systems, Landsat- 8/OLI, Lineaments, Principal component analysis, hydrothermally altered. DOI: 10.7176/JEES/9-11-03 Publication date: November 30th 201

Identification of production challenges and benefits using value chain mapping of egg food systems in Nairobi, Kenya

Commercial layer and indigenous chicken farming in Nairobi and associated activities in the egg value chains are a source of livelihood for urban families. A value chain mapping framework was used to describe types of inputs and outputs from chicken farms, challenges faced by producers and their disease control strategies. Commercial layer farms were defined as farms keeping exotic breeds of chicken, whereas indigenous chicken farms kept different cross breeds of indigenous chicken. Four focus group discussions were held with producers of these chickens in peri-urban area: Dagoretti, and one informal settlement: Kibera. Qualitative data were collected on interactions between farmers, sources of farm inputs and buyers of poultry products, simple ranking of production challenges, farmers' perception on diseases affecting chicken and strategies for management of sick chicken and waste products. Value chain profiles were drawn showing sources of inputs and channels for distribution of chicken products. Production challenges and chicken disease management strategies were presented as qualitative summaries. Commercial layer farms in Dagoretti kept an average of 250 chickens (range 50–500); while flock sizes in Kibera were 12 chickens (range 5–20). Farms keeping indigenous chicken had an average of 23 chickens (range 8–40) in Dagoretti, and 10 chickens (range 5–16) in Kibera. Commercial layer farms in Dagoretti obtained chicks from distributors of commercial hatcheries, but farms in Kibera obtained chicks from hawkers who in turn sourced them from distributors of commercial hatcheries. Indigenous chicken farms from Dagoretti relied on natural hatching of fertilised eggs, but indigenous chicken farms in Kibera obtained chicks from their social connection with communities living in rural areas. Outlets for eggs from commercial layer farms included local shops, brokers, restaurants and hawkers, while eggs from indigenous chicken farms were sold to neighbours and restaurants. Sieved chicken manure from Dagoretti area was fed to dairy cattle; whereas non-sieved manure was used as fertilizer on crops. Production challenges included poor feed quality, lack of space for expansion, insecurity, occurrence of diseases and lack of sources of information on chicken management. In Kibera, sick and dead chickens were slaughtered and consumed by households; this practice was not reported in Dagoretti. The chicken layer systems contribute to food security of urban households, yet they have vulnerabilities and deficiencies with regard to disease management and food safety that need to be addressed with support on research and extension