Facile and rapid synthesis of highly luminescent nanoparticles via Pulsed Laser Ablation in Liquid

This paper demonstrates the usefulness of pulsed laser ablation in liquids as a fast screening synthesis method able to prepare even complex compositions at the nanoscale. Nanoparticles of Y2O3:Eu3+, Lu2O2S: Eu3+, Gd2SiO5:Ce3+ and Lu3TaO7:Gd3+,Tb3+ are successfully synthesized by pulsed laser ablation in liquids. The phase and stoichiometries of the original materials are preserved while the sizes are reduced down to 5-10 nm. The optical properties of the materials are also preserved but show some small variations and some additional structures which are attributed to the specificities of the nanoscale (internal pressure, inhomogeneous broadening, surface states...

Predicting gene expression in the human malaria parasite Plasmodium falciparum using histone modification, nucleosome positioning, and 3D localization features.

Empirical evidence suggests that the malaria parasite Plasmodium falciparum employs a broad range of mechanisms to regulate gene transcription throughout the organism's complex life cycle. To better understand this regulatory machinery, we assembled a rich collection of genomic and epigenomic data sets, including information about transcription factor (TF) binding motifs, patterns of covalent histone modifications, nucleosome occupancy, GC content, and global 3D genome architecture. We used these data to train machine learning models to discriminate between high-expression and low-expression genes, focusing on three distinct stages of the red blood cell phase of the Plasmodium life cycle. Our results highlight the importance of histone modifications and 3D chromatin architecture in Plasmodium transcriptional regulation and suggest that AP2 transcription factors may play a limited regulatory role, perhaps operating in conjunction with epigenetic factors

Comprehensive Determination of the Hinode/EIS Roll Angle

We present a new coalignment method for the EUV Imaging Spectrometer (EIS) on board the Hinode spacecraft. In addition to the pointing offset and spacecraft jitter, this method determines the roll angle of the instrument, which has never been systematically measured, and is therefore usually not corrected. The optimal pointing for EIS is computed by maximizing the cross-correlations of the Fe XII 195.119 \r{A} line with images from the 193 \r{A} band of the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamics Observatory (SDO). By coaligning 3336 rasters with high signal-to-noise ratio, we estimate the rotation angle between EIS and AIA and explore the distribution of its values. We report an average value of (-0.387 $\pm$ 0.007)\deg. We also provide a software implementation of this method that can be used to coalign any EIS raster.Comment: Accepted for publication in Solar Physics, 11 pages, 7 figure

Analysis of Single Locus Trajectories for Extracting In Vivo Chromatin Tethering Interactions

Is it possible to extract tethering forces applied on chromatin from the statistics of a single locus trajectories imaged in vivo? Chromatin fragments interact with many partners such as the nuclear membrane, other chromosomes or nuclear bodies, but the resulting forces cannot be directly measured in vivo. However, they impact chromatin dynamics and should be reflected in particular in the motion of a single locus. We present here a method based on polymer models and statistics of single trajectories to extract the force characteristics and in particular when they are generated by the gradient of a quadratic potential well. Using numerical simulations of a Rouse polymer and live cell imaging of the MAT-locus located on the yeast Saccharomyces cerevisiae chromosome III, we recover the amplitude and the distance between the observed and the interacting monomer. To conclude, the confined trajectories we observed in vivo reflect local interaction on chromatin

Flavour-Changing Neutral Currents and Leptophobic Z' Gauge Bosons

Leptophobic Z' gauge bosons can appear in models with an E_6 gauge symmetry. We show that flavour-changing neutral currents can be generated in some of these models due to the mixing of the ordinary d_R, s_R and b_R quark fields with the exotic h_R. Because the Z' does not couple to charged leptons, the constraints on the flavour-changing couplings U^{Z'}_{db} and U^{Z'}_{sb} are relatively weak. Indeed, B_q--Bbar_q mixing (q=d,s) can be dominated by Z' exchange, which will affect CP-violating rate asymmetries in B decays. Rare hadronic B decays can also be affected, while decays involving charged leptons will be unchanged.Comment: 11 pages, LaTeX, no figure

Recent molecular approaches to understanding astrocyte function in vivo

Astrocytes are a predominant glial cell type in the nervous systems, and are becoming recognized as important mediators of normal brain function as well as neurodevelopmental, neurological, and neurodegenerative brain diseases. Although numerous potential mechanisms have been proposed to explain the role of astrocytes in the normal and diseased brain, research into the physiological relevance of these mechanisms in vivo is just beginning. In this review, we will summarize recent developments in innovative and powerful molecular approaches, including knockout mouse models, transgenic mouse models, and astrocyte-targeted gene transfer/expression, which have led to advances in understanding astrocyte biology in vivo that were heretofore inaccessible to experimentation. We will examine the recently improved understanding of the roles of astrocytes – with an emphasis on astrocyte signaling – in the context of both the healthy and diseased brain, discuss areas where the role of astrocytes remains debated, and suggest new research directions