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Part I—Dedication to Professor Allen F. Roberts
 
Construction of reference chromosome-scale pseudomolecules for potato:integrating the potato genome with genetic and physical maps
ArtĂculo de publicaciĂłn ISIThe genome of potato, a major global food crop, was recently sequenced. The work presented
here details the integration of the potato reference genome (DM) with a new sequence-tagged site
marker2based linkage map and other physical and genetic maps of potato and the closely related species
tomato. Primary anchoring of the DM genome assembly was accomplished by the use of a diploid segregating
population, which was genotyped with several types of molecular genetic markers to construct a new
~936 cM linkage map comprising 2469 marker loci. In silico anchoring approaches used genetic and
physical maps from the diploid potato genotype RH89-039-16 (RH) and tomato. This combined approach
has allowed 951 superscaffolds to be ordered into pseudomolecules corresponding to the 12 potato
chromosomes. These pseudomolecules represent 674 Mb (~93%) of the 723 Mb genome assembly and
37,482 (~96%) of the 39,031 predicted genes. The superscaffold order and orientation within the pseudomolecules
are closely collinear with independently constructed high density linkage maps. Comparisons
between marker distribution and physical location reveal regions of greater and lesser recombination, as
well as regions exhibiting significant segregation distortion. The work presented here has led to a greatly
improved ordering of the potato reference genome superscaffolds into chromosomal “pseudomolecules”
Optical technologies and molecular imaging for cervical neoplasia:a program project update
There is an urgent global need for effective and affordable approaches to cervical cancer screening and diagnosis. In developing nations, cervical malignancies remain the leading cause of cancer-related deaths in women. This reality may be difficult to accept given that these deaths are largely preventable; where cervical screening programs have been implemented, cervical cancerrelated deaths have decreased dramatically. In developed countries, the challenges of cervical disease stem from high costs and overtreatment. The National Cancer Institutefunded Program Project is evaluating the applicability of optical technologies in cervical cancer. The mandate of the project is to create tools for disease detection and diagnosis that are inexpensive, require minimal expertise, are more accurate than existing modalities, and can be feasibly implemented in a variety of clinical settings. This article presents the status and long-term goals of the project. © 2012 Elsevier HS Journals, Inc. All rights reserved.
, PhD8, MD11, MD12, PhD1,, MD, PhD14, 15, Roderick Price, MSc16, Isaac F. Adewole, MD17, Calum MacAulay, PhD1