Accumulation and bioaccessibility of trace elements in wetland sediments

Accumulation of trace metals in sediment can cause severe ecological impacts. In this study, determination of elemental concentrations in water and sediment was done. Shadegan wetland is one of the most important wetlands in southwest of Iran and is among the Ramsar-listed wetlands.Wastewaters from industries, urban activities and agricultural run off are released into this unique wetland in the region. In the present  investigation, 12 sampling stations were selected for water and sediment collection in Shadegan wetland during winter, 2009. Physico-chemical parameters such as pH, electrical conductivity (EC), turbidity and salinity of water and pH, EC, total organic materials (TOM), grain size fraction and elements (Al, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Pb, V and Zn) in sediments were measured. Also, macrobenthos communities were identified in sediments due to their important role in ecosystem health. Subsequently, lithogenous and anthropogenic portions of trace metals in sediment, trace elements bioaccessibility, along with Igeo and IPOLL indices were measured. According to obtained results, trace elements including Cu (39.5%), Pb (26%), Cr (24.1%), Cd (16.4%) and Zn (13.6%) had highest anthropogenic portion in study area. The two pollution indices used in the present investigation (Igeo and IPOLL) are indicative of different pollution intensity in Shadegan aquatic environment. The low pollution intensity values computed by Igeo might be acceptable for metals that have low anthropogenic values (less than 15% of their total concentrations). But higher anthropogenic values (16 to almost 40% of total concentration) for other studied metals show that an IPOLL pollutionintensity formula is more reliable. Moreover, bioaccessibility concentrations amount for Ni, V, Pb and Co had strong and positive meaningful  relationship with Cu, Cr and Cd.Key words: Heavy metals, accumulation, aquatic environment, IPOLL index, Shadegan wetland

Water quality index development using fuzzy logic: A case study of the Karoon River of Iran

Determination of the status of water quality of a river or any other water source is highly indeterminate. It is necessary to have a competent model to predict the status of water quality and to show the type of water treatment that would be used to meet different demands. By exploring the behavior and limitations of conventional methods for quality evaluation, a better overall index for water quality in Iran and its application in Karoon River is proposed. Six variables are employed for the quality assessment. Numerical scales relating to the degree of quality are established for each variable to assess variations in quality and to convey findings in a comprehensive manner. The unit operates in a fuzzy logic mode including a fuzzification engine receiving a plurality of input variables on its input and being adapted to compute membership function parameters. A processor engine connected downstream of the fuzzification unit will produce fuzzy set, based on fuzzy variable namely dissolved oxygen (DO), total dissolved solids (TDS), turbidity, nitrate, fecal coliform and pH. It has a defuzzification unit which operates to translate the inference results into a discrete crisp value of water quality index. The development of the fuzzy model with one river system is explained in this paper. Water quality index in most countries is only referring to physico-chemical parameters due to great efforts needed to quantify the biological parameters. This study ensures a better method to include special parameters into water quality index due to superior capabilities of fuzzy logic in dealing with non-linear, complex and uncertain systems.Key words: Water quality index, fuzzification, monitoring, inference system

The geoaccumulation index and enrichment factor of mercury in mangrove sediment of Port Klang, Selangor, Malaysia.

Mangrove areas are important to the ecosystem. One of its crucial functions is as a sink of pollutants, especially metal ions. However, the accumulation of metals in mangrove sediment can generate negative impacts on plant growth, microbial activity, and soil fertility. Apart from that, the severity of the impact is highly influenced by the type of metal found in the sediment and the quality of sediment itself. One of the metals that have adverse effects on the environment is mercury. The objectives of this study are to determine the concentration and distribution of mercury and to assess the enrichment of mercury in Port Klang mangrove sediment by using geoaccumulation index and enrichment factor. Sediment samples were collected from 30 sampling points that cover Langat River and Klang River estuaries, Lumut Straits, Pulau Klang, and Pulau Indah. During sampling, water parameters such as pH, salinity, electrical conductivity, and total dissolved solids were measured in situ, whereas the total mercury in sediment samples was determined at the laboratory using inductively coupled plasma mass spectrometry. In this study, mercury was found to be concentrated along Lumut Strait especially in the mixing zone near the confluence of Langat River and at the jetty to Pulau Ketam. The geoaccumulation index and enrichment factor (calculated using logarithmized data of the reference element) found that three stations were enriched with mercury. In addition, geoaccumulation index was also observed to be more objective compared to enrichment factor whose results were influenced by the concentration of reference element used

Integrated Assessment of Heavy Metal Contamination in Sediments from a Coastal Industrial Basin, NE China

The purpose of this study is to investigate the current status of metal pollution of the sediments from urban-stream, estuary and Jinzhou Bay of the coastal industrial city, NE China. Forty surface sediment samples from river, estuary and bay and one sediment core from Jinzhou bay were collected and analyzed for heavy metal concentrations of Cu, Zn, Pb, Cd, Ni and Mn. The data reveals that there was a remarkable change in the contents of heavy metals among the sampling sediments, and all the mean values of heavy metal concentration were higher than the national guideline values of marine sediment quality of China (GB 18668-2002). This is one of the most polluted of the world’s impacted coastal systems. Both the correlation analyses and geostatistical analyses showed that Cu, Zn, Pb and Cd have a very similar spatial pattern and come from the industrial activities, and the concentration of Mn mainly caused by natural factors. The estuary is the most polluted area with extremely high potential ecological risk; however the contamination decreased with distance seaward of the river estuary. This study clearly highlights the urgent need to make great efforts to control the industrial emission and the exceptionally severe heavy metal pollution in the coastal area, and the immediate measures should be carried out to minimize the rate of contamination, and extent of future pollution problems

Linear and Branched Glyco-Lipopeptide Vaccines Follow Distinct Cross-Presentation Pathways and Generate Different Magnitudes of Antitumor Immunity

Glyco-lipopeptides, a form of lipid-tailed glyco-peptide, are currently under intense investigation as B- and T-cell based vaccine immunotherapy for many cancers. However, the cellular and molecular mechanisms of glyco-lipopeptides (GLPs) immunogenicity and the position of the lipid moiety on immunogenicity and protective efficacy of GLPs remain to be determined.We have constructed two structural analogues of HER-2 glyco-lipopeptide (HER-GLP) by synthesizing a chimeric peptide made of one universal CD4(+) epitope (PADRE) and one HER-2 CD8(+) T-cell epitope (HER(420-429)). The C-terminal end of the resulting CD4-CD8 chimeric peptide was coupled to a tumor carbohydrate B-cell epitope, based on a regioselectively addressable functionalized templates (RAFT), made of four alpha-GalNAc molecules. The resulting HER glyco-peptide (HER-GP) was then linked to a palmitic acid moiety, attached either at the N-terminal end (linear HER-GLP-1) or in the middle between the CD4+ and CD8+ T cell epitopes (branched HER-GLP-2). We have investigated the uptake, processing and cross-presentation pathways of the two HER-GLP vaccine constructs, and assessed whether the position of linkage of the lipid moiety would affect the B- and T-cell immunogenicity and protective efficacy. Immunization of mice revealed that the linear HER-GLP-1 induced a stronger and longer lasting HER(420-429)-specific IFN-gamma producing CD8(+) T cell response, while the branched HER-GLP-2 induced a stronger tumor-specific IgG response. The linear HER-GLP-1 was taken up easily by dendritic cells (DCs), induced stronger DCs maturation and produced a potent TLR- 2-dependent T-cell activation. The linear and branched HER-GLP molecules appeared to follow two different cross-presentation pathways. While regression of established tumors was induced by both linear HER-GLP-1 and branched HER-GLP-2, the inhibition of tumor growth was significantly higher in HER-GLP-1 immunized mice (p<0.005).These findings have important implications for the development of effective GLP based immunotherapeutic strategies against cancers