The $5.50 per acre experiment

Non-Peer Reviewe

Dynamic interplay between breast cancer cells and normal endothelium mediates the expression of matrix macromolecules, proteasome activity and functional properties of endothelial cells

Background Breast cancer\u2013endothelium interactions provide regulatory signals facilitating tumor progression. The endothelial cells have so far been mainly viewed in the context of tumor perfusion and relatively little is known regarding the effects of such paracrine interactions on the expression of extracellular matrix (ECM), proteasome activity and properties of endothelial cells. Methods To address the effects of breast cancer cell (BCC) lines MDA-MB-231 and MCF-7 on the endothelial cells, two cell culture models were utilized; one involves endothelial cell culture in the presence of BCCs-derived conditioned media (CM) and the other co-culture of both cell populations in a Transwell system. Real-time PCR was utilized to evaluate gene expression, an immunofluorescence assay for proteasome activity, and functional assays (migration, adhesion and invasion) and immunofluorescence microscopy for cell integrity and properties. Results BCC-CM decreases the cell migration of HUVEC. Adhesion and invasion of BCCs are favored by HUVEC and HUVEC-CM. HA levels and the expression of CD44 and HA synthase-2 by HUVEC are substantially upregulated in both cell culture approaches. Adhesion molecules, ICAM-1 and VCAM-1, are also highly upregulated, whereas MT1-MMP and MMP-2 expressions are significantly downregulated in both culture systems. Notably, the expression and activity of the proteasome \u3b25 subunit are increased, especially by the action of MDA-MB-231-CM on HUVEC. Conclusions and general significance BCCs significantly alter the expression of matrix macromolecules, proteasome activity and functional properties of endothelial cells. Deep understanding of such paracrine interactions will help to design novel drugs targeting breast cancer at the ECM level. This article is part of a Special Issue entitled Matrix-mediated cell behavior and properties

Evaluation of seven barley genotypes under water stress conditions

Received: July 4th, 2022 ; Accepted: November 27th, 2022 ; Published: December 23rd, 2022 ; Correspondence: [email protected] evaluation of seven barley genotypes under water stress conditions using drought tolerance indices was investigated during two agronomical seasons in the experimental field of Agricultural University of Athens in Greece. The experimental design was a split-plot layout in four blocks. Four different levels of irrigation were implemented, with the method of escalated distance from the source of water (drip irrigation line). The experimental plots were protected from rain since the experiment was conducted under a rainout shelter. Measurements of water potential index of the plants were conducted, as well as stomatal resistance and stomatal resistance index of leaves, and grain yield of genotypes. A decrease in water potential index (15–25%) and grain yield (35–54%) was observed in all genotypes as soil moisture decreased. On the other hand, stomatal resistance and stomatal resistance index (26–69%) of leaves increased. Grain yield had a strong relationship with the indices of water potential and stomatal resistance of leaves. Grain yield of all genotypes is affected under water stress conditions, with the six-rowed genotypes being more adaptive than the two-rowed ones. It can be concluded that indices of water potential and stomatal resistance of leaves can be effectively used in the evaluation of genotypes under water stress conditions

The impact of DCD and NBPT concentration on nitrification and volatilization

Non-Peer ReviewedNitrification inhibitors are used in agriculture to slow the conversion of ammonium to nitrate by inhibiting ammonia monoxygenace (AMO), which is found within the Nitrosomonas bacteria. There are two ways to inhibit nitrification of the AMO enzyme, namely, (a) competitive inhibition: The inhibitor will compete to occupy AMO's active site; by blocking the active site, it slows the conversion of ammonium to nitrate preventing loss of nitrates, and, (b) non-competitive inhibition: The inhibitor can temporarily inactivate the AMO enzyme by altering the active site. Dicyandiamide (DCD) is a competitive inhibitor. The amount of DCD delivered by a product has a direct impact on how well a product will inhibit loss of nitrogen through nitrification. Research shows the concentration of DCD in the soil has a direct influence on the percent of nitrification. Higher concentrations of DCD equate to higher percentage of nitrification inhibition. The results of a two-year program that compares two products, one containing 8500 ppm and one 870ppm of DCD and approximately equal concentration of a volatilization inhibitor will be presented

Expression and Localization of Glycosaminoglycans/Proteoglycan in Pterygium: An Immunohistochemical Study

Pterygium is a triangle-shaped fibrovascular hyperplasia of the bulbar conjunctiva on the cornea. The purpose of this study was to analyze Proteoglycans (PGs) by Immunohistochemistry (IHC) in pterygium tissues and to compare the results with normal conjunctiva. Twenty-four patients (14 males) undergoing primary pterygium excision and 17 healthy individuals (10 males), undergoing extracapsular cataract surgery, were included. Pterygium tissues and normal conjunctiva tissues were surgically removed. The tissue sections were fixed in 2% paraformaldehyde and incubated with monoclonal antibodies against PGs anti-mouse IgG. Immunohistochemical study showed stronger expression of keratan sulfate in the stroma of the pterygium compared to normal conjunctiva. An increased expression of heparan sulfate was observed in the epithelial layer and around the pterygium vessels. On the other hand, dermatan sulfate showed an increased expression and localization not only in the sub-epithelial area of the pterygium and normal conjunctiva, yet throughout the stroma of the pterygium. The differences in the expression and localization of the studied extracellular matrix proteoglycans in the pterygium tissue compared to normal conjunctiva may explain the tissue hyperplasia, structure, and the functional properties in pterygium

Colossal enhancement of the magnetic dipole moment by exploiting lattice coupling in metasurfaces

An artificial magnetic response is not only intellectually intriguing but also key to multiple applications. While previously suitably structured metallic particles and high-permittivity dielectric particles have been used for this purpose, here, we highlight the possibility of exploiting lattice effects to significantly enhance an intrinsically weak magnetic dipole moment of a periodically arranged scatterer. We identify the effective magnetic dipole moment as it is modulated by the lattice and coupled to other electromagnetic multipole moments the scatterer can sustain. Besides a more abstract consideration on the base of parametrized Mie coefficients to study the theoretical upper limit, we present an actual particle that shows an enhancement of the magnetic dipole moment by 100 with respect to what is attainable as a maximal value for an isolated particle

Endovascular Treatment of Popliteal Artery Aneurysms

AbstractObjective. To present the results of the endovascular treatment of popliteal artery aneurysms.Methods. From April 1999 to January 2002, 11 patients, aged 40–94 years, with 12 popliteal aneurysms were treated. Nine (75%) underwent an endoluminal repair, of whom three were done emergently due to a aneurysm rupture. Aneurysm diameter was 28–105 (mean 69) mm. A Hemobahn stent graft was inserted in six, Wallgraft in two and Passager in one case.Results. During a mean follow-up of 14 (3–31) months, four (44%) thromboses occurred: two in the early postoperative period (30 days) and two during the late postoperative period. Two of the four occluded grafts were successfully reopened, and in the one a stenosis of the distal end of the stent graft was treated with balloon dilatation. Patency rates at 1 and 12 months were 64/47% (primary patency) and 88/75% (secondary patency), respectively.Conclusion. Initial experience with endovascular treatment of the popliteal aneurysm in high-risk patients yielded modest results. Larger number of patients and further follow-up time is necessary to evaluate the long-term results

Inverse Design of All-dielectric Metasurfaces with Bound States in the Continuum

Metasurfaces with bound states in the continuum (BICs) have proven to be a powerful platform for drastically enhancing light-matter interactions, improving biosensing, and precisely manipulating near- and far-fields. However, engineering metasurfaces to provide an on-demand spectral and angular position for a BIC remains a prime challenge. A conventional solution involves a fine adjustment of geometrical parameters, requiring multiple time-consuming calculations. In this work, to circumvent such tedious processes, we develop a physics-inspired, inverse design method on all-dielectric metasurfaces for an on-demand spectral and angular position of a BIC. Our suggested method predicts the core-shell particles that constitute the unit cell of the metasurface, while considering practical limitations on geometry and available materials. Our method is based on a smart combination of a semi-analytical solution, for predicting the required dipolar Mie coefficients of the meta-atom, and a machine learning algorithm, for finding a practical design of the meta-atom that provides these Mie coefficients. Although our approach is exemplified in designing a metasurface sustaining a BIC, it can, also, be applied to many more objective functions. With that, we pave the way toward a general framework for the inverse design of metasurfaces in specific and nanophotonic structures in general.Comment: 20 pages, 5 figures, Supplementary Materia

ESR2 Drives Mesenchymal-to-Epithelial Transition in Triple-Negative Breast Cancer and Tumorigenesis In Vivo

Estrogen receptors (ERs) have pivotal roles in the development and progression of triple-negative breast cancer (TNBC). Interactions among cancer cells and tumor microenvironment are orchestrated by the extracellular matrix that is rapidly emerging as prominent contributor of fundamental processes of breast cancer progression. Early studies have correlated ERβ expression in tumor sites with a more aggressive clinical outcome, however ERβ exact role in the progression of TNBC remains to be elucidated. Herein, we introduce the functional role of ERβ suppression following isolation of monoclonal cell populations of MDA-MB-231 breast cancer cells transfected with shRNA against human ESR2 that permanently resulted in 90% reduction of ERβ mRNA and protein levels. Further, we demonstrate that clone selection results in strongly reduced levels of the aggressive functional properties of MDA-MB-231 cells, by transforming their morphological characteristics, eliminating the mesenchymal-like traits of triple-negative breast cancer cells. Monoclonal populations of shERβ MDA-MB-231 cells undergo universal matrix reorganization and pass on a mesenchymal-to-epithelial transition state. These striking changes are encompassed by the total prevention of tumorigenesis in vivo following ERβ maximum suppression and isolation of monoclonal cell populations in TNBC cells. We propose that these novel findings highlight the promising role of ERβ targeting in future pharmaceutical approaches for managing the metastatic dynamics of TNBC breast cancer

Dietary fat intake as a risk factor for the development of diabetes. Multinational, multicenter study of the Mediterranean Group for the Study of Diabetes (MGDS)

In the context of the Multinational MGSD Nutrition Study, three groups of subjects were studied: 204 subjects with recently diagnosed diabetes(RDM),42subjectswithundiagnoseddiabetes(UDM)(AmericanDiabetesAssociation criteria—fasting plasma glucose [FPG] 126 mg/dl), and 55 subjects with impaired fasting glucose(IFG)(FPG 110and126mg/dl).Eachgroupwascomparedwithacontrolgroupof nondiabetic subjects, matched one by one for center, sex, age, and BMI. Nutritional habits were evaluated by a dietary history method, validated against the 3-day diet diary. In RDM, the questionnaire referred to the nutritional habits before the diagnosis of diabetes. Demographic data were collected, and anthropometrical and biochemical measurements were taken. RESULTS— Compared with control subjects, RDM more frequently had a family history of diabetes(49.0vs.14.2%;P0.001),exercisedless(exerciseindex53.5vs.64.4;P0.01),and more frequently had sedentary professions (47.5 vs. 27.4%; P 0.001). Carbohydrates contributed less to their energy intake (53.5 vs. 55.1%; P 0.05), whereas total fat (30.2 0.5 vs. 27.8 0.5%; P 0.001) and animal fat (12.2 0.3 vs. 10.8 0.3%; P 0.01) contributed moreandtheplant-to-animalfatratiowaslower(1.50.1vs.1.80.1;P0.01).UDMmore frequentlyhadafamilyhistoryofdiabetes(38.1vs.19.0%;P0.05)andsedentaryprofessions (58.5vs.34.1%;P0.05),carbohydratescontributedlesstotheirenergyintake(47.61.7vs. 52.81.4%;P0.05),totalfat(34.71.5vs.30.41.2%;P0.05)andanimalfat(14.2 0.9 vs. 10.6 0.7%; P 0.05) contributed more, and the plant-to-animal fat ratio was lower (1.6 0.2 vs. 2.3 0.4; P 0.05). IFG differed only in the prevalence of family history of diabetes (32.7 vs. 16.4%; P 0.05). CONCLUSIONS— Our data support the view that increased animal fat intake is associated with the presence of diabetes