Kinematic characteristics of hailstorms in Northern Greece

International audienceThe purpose of this study is the analysis of radar data, digitally recorded, during an operational hail suppression program in the region of Central Macedonia, Greece, for the warm period of the years 1997?2001. Kinematic characteristics, such as lifetime and distance traveled by hailstorms, as well as direction of motion and speed, have been related to type of storms and season. It has been found that singlecells are short-lived and travel short distances, while multicells are long-lived and travel long distances. On the contrary, their corresponding speed distributions are similar. The deviation of the direction of motion from mean wind is smaller for singlecells than for multicells. September and July exhibit the maximum and minimum average storm speeds as a direct implication of synoptic disturbances passage and convection, prevailing respectively. Finally, storms overcoming orographic barriers decelerate in general on the windward side and accelerate on the lee side of mountains

MODELING THE TRANS-ATLANTIC TRANSPORTATION OF SAHARAN DUST

In the present study we are simulating the trans-Atlantic transport of dust from Sahara to the South-Central America, using the regional climate model RegCM4 and its online dust scheme, for the year 2007. The simulated horizontal and vertical distributions of the mineral dust optical properties were evaluated against the LIVAS CALIPSO satellite dust product. The Trans-Atlantic dust transport is simulated adequately with RegCM4, but there are some spatial discrepancies. Dust optical thickness is overestimated in the eastern Sahara throughout the year by 0.1-0.2, while near the gulf of Guinea is underestimated during winter and spring. Although RegCM4 dust plume is located southern on winter and spring, it doesn't spatially match the dust optical thickness of LIVAS. In summer and autumn the vertical distribution of dust between 3-4km during the Trans-Atlantic transport is simulated by the model adequately up to 30ºW 40ºW longitude. However, during winter-spring RegCM4 misplaces dust loading into higher altitude. Finally, we discuss some possible reasons and mechanisms that might be responsible for the differences between the model and the observations

Mutations in matrix and SP1 repair the packaging specificity of a Human Immunodeficiency Virus Type 1 mutant by reducing the association of Gag with spliced viral RNA

<p>Abstract</p> <p>Background</p> <p>The viral genome of HIV-1 contains several secondary structures that are important for regulating viral replication. The stem-loop 1 (SL1) sequence in the 5' untranslated region directs HIV-1 genomic RNA dimerization and packaging into the virion. Without SL1, HIV-1 cannot replicate in human T cell lines. The replication restriction phenotype in the SL1 deletion mutant appears to be multifactorial, with defects in viral RNA dimerization and packaging in producer cells as well as in reverse transcription of the viral RNA in infected cells. In this study, we sought to characterize SL1 mutant replication restrictions and provide insights into the underlying mechanisms of compensation in revertants.</p> <p>Results</p> <p>HIV-1 lacking SL1 (NLΔSL1) did not replicate in PM-1 cells until two independent non-synonymous mutations emerged: G913A in the matrix domain (E42K) on day 18 postinfection and C1907T in the SP1 domain (P10L) on day 11 postinfection. NLΔSL1 revertants carrying either compensatory mutation showed enhanced infectivity in PM-1 cells. The SL1 revertants produced significantly more infectious particles per nanogram of p24 than did NLΔSL1. The SL1 deletion mutant packaged less HIV-1 genomic RNA and more cellular RNA, particularly signal recognition particle RNA, in the virion than the wild-type. NLΔSL1 also packaged 3- to 4-fold more spliced HIV mRNA into the virion, potentially interfering with infectious virus production. In contrast, both revertants encapsidated 2.5- to 5-fold less of these HIV-1 mRNA species. Quantitative RT-PCR analysis of RNA cross-linked with Gag in formaldehyde-fixed cells demonstrated that the compensatory mutations reduced the association between Gag and spliced HIV-1 RNA, thereby effectively preventing these RNAs from being packaged into the virion. The reduction of spliced viral RNA in the virion may have a major role in facilitating infectious virus production, thus restoring the infectivity of NLΔSL1.</p> <p>Conclusions</p> <p>HIV-1 evolved to overcome a deletion in SL1 and restored infectivity by acquiring compensatory mutations in the N-terminal matrix or SP1 domain of Gag. These data shed light on the functions of the N-terminal matrix and SP1 domains and suggest that both regions may have a role in Gag interactions with spliced viral RNA.</p

Growth and Feed Utilization by Golden Grey Mullet (Liza aurata) in a Coastal Lagoon Ecosystem, Fed Compound Feeds with Varying Protein Levels

The effects of complimentary compound feeds with varying protein levels on growth performance, feed utilization, and whole body composition were studied in golden grey mullet, Liza aurata. Fish of initial weight 51.1 +/- 5.2 g were raised in hapas in a coastal lagoon and fed four iso-energetic diets containing 25%, 30%, 35% or 45% of crude protein for 20 weeks. L. aurata reared on the experimental diets showed relatively low growth rates (SGR ranged from 0.40 to 0.48%/day, and feed utilization parameters (FE ranged from 0.27 to 0.34) among dietary groups consistent with the known slow growth rate of the species. The growth performance and feed utilization of L. aurata was not significantly affected by dietary protein level, despite the fact that there was a trend indicating better fish growth and feed efficiency with increasing dietary protein level. This is indicative of the low protein requirements of L. aurata, although it could be that higher dietary protein concentrations could possibly improve growth performance. The farming of low-trophic species such as L. aurata allows under-utilized trophic resources to be better exploited for fish production. Thus any future advances in understanding the nutrition and feeding of L. aurata under culture conditions, could improve further the potential of this species, especially in regional and coastal aquaculture

Fog characteristics at the airport of Thessaloniki, Greece

A statistical approach in order to study fog event characteristics occuring at the airport of Thessaloniki, Northern Greece is presented in this work. To achieve this, the seasonal and diurnal characteristics of fog are investigated using 35 years (1971–2005) of observations of meteorological parameters such as: visibility, air temperature, dew point temperature, air relative humidity, wind vector, precipitation, and cloud base height and coverage. Hourly surface observations of fifteen (15) years (1991–2005) are used to identify fog events induced by various physical mechanisms. Fog events are classified into fog types through the application of objective criteria that are derived upon fog formation processes and under the influence of various physiographic features. The temporal variability of different fog type occurrences are examined and the events are characterized according to their duration and intensity. &lt;br&gt;&lt;br&gt; The results are somehow affected by regional and local factors. Fog is mainly formed in winter time (~64%) with an enhanced likelihood to appear also in late autumn (19%). The highest frequency of the fog events occurs around sunrise or 1 to 2 h before of it. The mean duration of the events is about 4.5 h. Most of them (75%) are dense (visibility &amp;lt;400 m). The overall fog phenomenon is a combination of various types, such as: advection fog, radiation fog, cloud-base lowering fog and precipitation fog. Advection fog (30%) and radiation fog (29%), which are the most common types, occur predominantly in winter and early spring time. With respect to the former type, it seems that, in many cases, already formed fog is advected from the nearby &lt;i&gt;Anthemountas&lt;/i&gt; valley. Moreover, a considerable number of fog events (22%) result from cloud-base lowering, and they frequently occur in late autumn and mid winter

Impact of dust size parameterizations on aerosol burden and radiative forcing in RegCM4

We investigate the sensitivity of aerosol representation in the regional climate model RegCM4 for two dust parameterizations for the period 2007–2014 over the Sahara and the Mediterranean. We apply two discretization methods of the dust size distribution keeping the total mass constant: (1) the default RegCM4 4-bin approach, where the size range of each bin is calculated using an equal, logarithmic separation of the total size range of dust, using the diameter of dust particles, and (2) a newly implemented 12-bin approach with each bin defined according to an isogradient method where the size ranges are dependent on the dry deposition velocity of dust particles. Increasing the number of transported dust size bins theoretically improves the representation of the physical properties of dust particles within the same size bin. Thus, more size bins improve the simulation of atmospheric processes. The radiative effects of dust over the area are discussed and evaluated with the CALIPSO dust optical depth (DOD). This study is among the first studies evaluating the vertical profile of simulated dust with a pure dust product. Reanalysis winds from ERA-Interim and the total precipitation flux from the Climate Research Unit (CRU) observational gridded database are used to evaluate and explain the discrepancies between model and observations. The new dust binning approach increases the dust column burden by 4 and 3 % for fine and coarse particles, respectively, which increases DOD by 10 % over the desert and the Mediterranean. Consequently, negative shortwave radiative forcing (RF) is enhanced by more than 10 % at the top of the atmosphere and by 1 to 5 % on the surface. Positive longwave RF locally increases by more than 0.1 W m⁻² in a large portion of the Sahara, the northern part of the Arabian Peninsula and the Middle East. The four-bin isolog method is to some extent numerically efficient, nevertheless our work highlights that the simplified representation of the four-bin approach produces less dust optical depth and RF, a fact that should be taken into account by future studies of the same region