The role of histological components and differentiation on survival ın Renal Wilms' tumor

Amaç: Wilms tümörü böbreğin çocukluk çağında görülen en sık malign tümörüdür. Prognoz yeni gelişmelerle giderek iyileşmektedir. Bir trifazik embriyonel tümör olan Wilms tümöründe bulunabilen her üç histolojik komponent (blastemal, stromal, epitelial) değişik miktar ve diferansiyasyonlarda gözlenebilmektedir. Bu çalışmanın amacı çocukluk çağı renalWilms tümöründe histolojik komponentlerin ve diferansiyasyon varlığının sağkalım üzerine etkisini araştırmaktır. Yöntem: Dr. Behçet Uz Çocuk Hastanesinde izlenen ve 1989-1997 yıllarında tanı almış 30 renalWilms tümörü olgusu bu çalışmada yer almaktadır. Olguların yaş ortalaması 51 aydır. 13 olgu erkek, 17 olgu kız çocuğudur. 8 olgu kötü prognozludur (exitus). Bulgular: 4 yıllık sağkalım %73.3' dür. Bir monofazik (blastemal), 7 bifazik tümör vardır. 22 olgu trifaziktir. 14 olguda mezenkimal, 9 olguda blastemal, 7 olguda tübüler komponent baskın bulunmuştur. 8 olguda mezenkimal komponentte çizgili kas diferansiyasyonu, 1 olguda kondroid, 1 olguda skuamöz diferansiyasyon gözlenmiştir. Sonuç: ıstatistiksel olarak bu seride diferansiyasyon varlığının, tümörün histolojik komponentleri yüzdesinin ya da baskınlığının sağkalımla ilişkisi saptanmamıştır.Wilms tümöründe bilinen ve yeni prognostik faktörlerin araştırılmasının izlem ve tedaviyi yönlendirmede yararlı olacağı sonucuna varılmıştır.Objective: Wilms' tumor is the most common malignant neoplasm of kidney in childhood. The patients enjoy a better prognosis by new advances in medicine. Histologically, the classic Wilms' tumor has a triphasic appearance, composed of variable mixtures of blastemal, epithelial and stromal elements. Stromal or epithelial elements may show different levels of differantiation. The aim of this study is to evaluate the role of histological components and the presence of differentiation on survival in pediatric renal Wilms' tumors. Materials and methods: This study includes 30 cases of renal Wilms' tumor diagnosed and followed at Dr. Behçet Uz Children's State Hospital between 1989 and 1997. The mean age of patients was 51 months. Thirteen casesweremaleand 17 caseswere female. Poorprognosis (exitus)was observedin 8 cases. Results: The 4 year survival rate was %73,3. One case was monomorphic (blastemal) while 7 cases were biphasic and 22 cases triphasic. The stromal component was dominant in 14 cases, blastemal component in 9 cases and epithelial component in 7 cases. Striated muscle was observed in 8 cases, cartilage in 1 case and squamous tissue in1 case. Conclusion: Statistical analysis showed no relationship between survival rate, histologic components and occurance of differantiation in this series. We conclude that studying the known and new prognostic factors in Wilms' tumor will be useful in the assessment of patients

Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis

<p>Abstract</p> <p>Background</p> <p>The ubiquitin proteasome system (UPS) is a key player in regulating many cellular processes via proteasomal degradation of ubiquitinated proteins. Recently published data show that Jab1/CSN5 interacts with p97/VCP and controls the ubiquitination status of proteins bound to p97/VCP in mouse and human cells. However, coexpression of p97/VCP and Jab1/CSN5 in the developing rat testis and epididymis has not previously been studied.</p> <p>Methods</p> <p>Testicular and epididymal tissues from 5-, 15-, 30-, and 60-day-old rats were examined by immunohistochemistry and Western blotting. Colocalisation of proteins was determined by immunofluorescence microscopy.</p> <p>Results</p> <p>In the 5-day-old rat testis, p97/VCP and Jab1/CSN5 were specifically expressed in gonocytes. The expression of p97/VCP and Jab1/CSN5 significantly increased at day 15 and was found in spermatogonia, Sertoli cells and spermatocytes. In 30- and 60-day-old rat testes, p97/VCP indicated moderate to strong expression in Sertoli cells, spermatogonia, round and elongating spermatids. However, moderate to weak expression was observed in spermatocytes. Jab1/CSN5 showed strong expression in spermatogonia and spermatocytes, while relatively moderate expression was observed in round and elongating spermatids in 30- and 60-day-old rat testes. In contrast, in the epididymis, the expression of both proteins gradually increased from 5 to 60 days of age. After rats reached 2 weeks of age, the expression of both proteins was mostly restricted to the basal and principal cells of the caput epididymis.</p> <p>Conclusions</p> <p>Our study suggests that p97/VCP and Jab1/CSN5 could be an important part of the UPS in the developing rat testis and epididymis and that both proteins may be involved in the regulation of spermatogenesis and epididymal epithelial functions.</p

Investigation of Relationships Between Bread Volume and Some Quality Parameters in Common Wheat (Triticum aestivum L.) Genotypes

Bu çalışmada; 2010-2011 ürün yılında 6 lokasyonda yetiştirilen 9 denemeden elde edilen ekmeklik buğday genotipinde, ekmek hacmi ile bazı kalite parametreleri arasındaki ilişkiler incelenmiştir. Araştırmada; hektolitre ve bin tane ağırlığı, tane sertliği, un verimi, Zeleny ve beklemeli Zeleny sedimentasyon değerleri, protein oranı, alveograf (W, P, L, G), farinograf (stabilite, yumuşama derecesi, su absorbsiyonu) ve glutograf parametreleri, yaş gluten, kuru gluten, gluten indeks, düşme sayısı, ekmek hacmi ve ağırlığı değerleri belirlenmiş, ekmek hacmi ile diğer parametreler arasındaki korelasyonlar incelenmiştir. Çalışma sonucunda ekmek hacmi ile yaş ve kuru gluten miktarı, Zeleny ve beklemeli Zeleny sedimentasyon değeri, tane protein oranı, ekmek ağırlığı ve alveograf parametreleri (W, G, L) ile pozitif korelasyon (p<0.01) değerleri elde edilirken, hektolitre ağırlığı, bin tane ağırlığı ve un veririmi ile negatif (p<0.01) korelasyon değerleri belirlenmiştir. Ayrıca, ekmek hacmi ile önemli korelasyon katsayısına sahip kalite parametreleri ve ekmek hacmi arasındaki regresyon da incelenmiş ve en yüksek belirtme katsayısına (R2) sahip regresyon eşitlikleri kuru ve yaş gluten parametrelerinden elde edilmiştir.In this study, the relationship between bread volume and some quality parameters of bread wheat genotypes belonging to 9 trials grown in 6 locations in 2010-2011 growing season were investigated. In the study, hardness, test and thousand kernel weights, flour yield, Zeleny and modified Zeleny sedimentation, protein content, alveograph (W, P, L, G) and farinograph (stability, softening degree, water absorption) parameters, wet and dry glutens, gluten index, falling number, bread volume and weight values were determined and correlations between bread volume and other parameters were investigated. According to results, positive (p<0.01) correlations between bread volume and Zeleny sedimentation, modified Zeleny sedimentation, grain protein content, alveograph parameters (W, L, G), wet gluten, dry gluten, bread weight were determined while there were negative (p<0.01) correlations between bread volume and test weight, thousand kernel weight, flour yield. Beside, regressions relationships were examined between bread volume and parameters having significant correlations with bread volume. Regression equations having the highest coefficients of determination (R2) were obtained from dry and wet gluten parameters

Chilaiditi syndrome in a child with cerebral palsy presenting with recurrent abdominal pain

Introduction: The hepatodiafragmatic interposition of colon is called Chilaiditi sign. For symptomatic patients, the term “Chilaiditi syndrome” is used, this term was first reported in 1911 by Demetrius Chilaiditi, a Viennese radiologist, in three asymptomatic patients. Patients are commonly asymptomatic, and some patients may apply with gastrointestinal or respiratory symptoms such as abdominal pain or chest pain. In the study of Lekkas et al, the incidence of Chilaiditi syndrome in mental retarded patients was reported to be 63 times higher than normal population.Case Presentation: High liver dome and elevated right diaphragm, hepatic flexura replaced between liver and anterior wall of abdomen were observed in chest radiography and abdominal computer tomography of our 6,5 year old male patient with cerebral palsy who has mental retardation and recurrent abdominal pain in medical history.Conclusions: In conclusion, Chilaiditi syndrome should be considered in mental retarded children with recurrent abdominal pain.Keywords: Child, chilaiditi syndrome, recurrent abdominal pain, mental retardation, cerebral pals

Investigation of the relationship between Zeleny sedimentation analysis and other quality parameters in bread wheat (Triticum aestivum L.)

Bu çalışmada, ekmeklik buğday genotiplerinde fiziksel, kimyasal ve reolojik kalite özellikleri incelenmiştir. Islahın erken kademesinde materyal miktarı yetersiz olduğu için kalitenin belirlenmesinde Zeleny sedimantasyon analizi önem kazanmaktadır. Çalışmada, Zeleny sedimantasyon değeri ile diğer kalite parametreleri arasındaki ilişki değerlendirilmiştir. 2015-2016 Yetiştirme sezonunda sulu koşullarda 4 lokasyonda kurulan denemelerden elde edilen materyal kullanılmıştır.1000 tane ağırlığı, hektolitre ağırlığı, tanede protein miktarı, un verimi, yaş gluten, kuru gluten ve gluten indeksi, alveograf parametreleri (G, P, L, P/G, P/L ve W), Zeleny sedimentasyon ve beklemeli Zeleny sedimantasyon değerleri, farinograf parametreleri (su absorbsiyonu ve yumuşama değeri) ile glutograf değerleri (stretch s, stretch BU ve relaxation BU) değerleri belirlenmiştir. Zeleny sedimantasyon değerleri ile aralarındaki korelasyonlar incelenmiştir. Araştırma sonucunda; Zeleny sedimantasyon değeri ile alveograf (G, L, P ve W), beklemeli Zeleny sedimantasyon değeri, farinograf su absorbsiyonu, tanede protein, yaş gluten ve kuru gluten değerleri (p<0,01), glutograf stretch (s) ve un verimi (p<0,05) arasında pozitif; farinograf yumuşama değeri arasında negatif (p<0,01) korelasyon değerleri elde edilmiştir. Zeleny sedimantasyon değeri ile yüksek korelasyon değerlerine sahip kalite parametreleri arasındaki regresyon incelendiğinde, en yüksek belirtme katsayısına (R2) sahip regresyon eşitlikleri alveograf enerji değeri (W), tanede protein ve kuru gluten parametrelerinden elde edilmiştir.In this research physical, chemical and rheological quality properties were examined in bread wheat genotypes. In early generation of plant breeding due to the material inadequacy, Zeleny sedimentation analyze is important in order to determine the quality. In this study, relationship between Zeleny sedimentation value and other quality parameters were investigated. Material was obtained from trials which were carried out in four locations on irrigated conditions in 2015-2016 growing season.Thousand kernel weight, test weight, grain protein content, flour yield, wet gluten, dry gluten and gluten index, alveograph parameters (G, P, L, P/G, P/L and W), Zeleny sedimentation and modified Zeleny sedimentation values, farinograph parameters (water absorption and softening degree) and glutograph values (stretch s, stretch BU and relaxation BU) were determined. Correlation coefficients between Zeleny sedimentation values and these parameters were investigated.Results showed that positive correlation coefficients were found between Zeleny sedimentation value and alveograph parameters (G, L, P and W), modified Zeleny sedimentation value, farinograph water absorption, grain protein content, wet gluten and dry gluten contents (p <0,01), glutograph stretch (s) and flour yield (p <0.05); negative correlation coefficients were obtained between Zeleny sedimentation value and farinograph softening degree (p <0,01). By examining the regressions between the Zeleny sedimentation value and the quality parameters with high correlation coefficient values, regression equations of highest determination coefficients (R2) were obtained from alveograph energy value (W), grain protein content and dry gluten parameters with the from

Evaluation the cases of neonatal sepsis and of antibiotic sensitivities in a neonatal intensive care unit

AMAÇ: Yenidoğan yoğun bakım ünitelerinde (YYBÜ) sepsis etkenleri ve antibiyotik duyarlılıkları zaman içinde ve kullanılan antibiyotiklere bağlı olarak değişir. Neonatal sepsise neden olan bakteriyel mikroorganizmaların ve antibiyotik duyarlılığının saptanması, ampirik antibiyotik tedavi rejimlerinin belirlenmesi ve uygun antibiyotik seçimi açısından önemlidir. GEREÇ veYÖNTEM: Ocak 2004-Haziran 2008 tarihleri arasında Adnan Menderes Üniversitesi Tıp Fakültesi YYBÜ'de izlenen 900 yenidoğan içerisinde sepsis tanısı alan 87 olgunun dosyaları incelendi. Kültür pozitif olan 45 olgunun klinik belirti ve bulguları, risk faktörleri, laboratuvar sonuçları, kültürde üreyen etkenler ve antibiyotik duyarlılıkları değerlendirildi. BULGULAR: Sepsis sıklıgı %9.6 bulundu. Kesin sepsis tanısı alan 45 olguda (%5), 49 kan kültürü üremesi oldu. Bunların %82'si prematüre ve%53'ü erkekti. Erken neonatal sepsiste Koagülaz Negatif Stafilokok (KNS) ve Staphylococcus aureus (S. aureus), geç neonatal sepsiste ise KNS'den sonra Candida spp. en sık üreyen mikroorganizmalardı. Mortalite oranı %13.3'tü. KNS'ler glikopeptidlere %100 duyarlı bulunurken, penisiline %96, metisiline %90 dirençliydi. S. aureus suslarının tamamı glikopeptidlere, metisiline, eritromisine ve klindamisine duyarlı bulunurken, penisiline direnç oranı %71.4 idi. Enterococcus spp. suslarında test edilen antibiyotiklere direnç saptanmadı. Enterobacter spp. susları 3. kusak sefalosporinlere ve karbapenemlere %100 duyarlı iken ampisilin-sulbaktama %100 dirençliydi. Acinetobacter spp. ve Pseudomonas spp. suslarının tamamı piperasiline dirençliydi. Klebsiella spp suslarının tamamı karbapenemlere, kinolonlara ve aminoglikozidlere duyarlı idi. Serratia spp., Escherichia coli ve Enterococcus spp. suslarında test edilen antibiyotiklere direnç saptanmadı. SONUÇ: Erken neonatal sepsiste Gram pozitif mikroorganizmaların ön plana geçmesi ve bu mikroorganizmalarda yüksek penisilin direnci görülmesi, ampirik antibiyotik uygulamalarının gözden geçirilmesi gerektiğini düşündürmektedir. Bununla birlikte S. aureus suslarında metisilin direncine rastlanmamıştır. Acinetobacter spp. ve Pseudomonas spp. suslarındaki yüksek piperasilin direnci önceki yıllarda bu antibiyotiğin sık kullanımına bağlandı.PURPOSE: Type and antibiotic sensitivity of pathogens leading sepsis in neonatal intensive care units (NICU) change over years. It is important to identify type and antibiotic sensitivity of pathogens leading sepsis for establishment of the local antibiotic policy. MATERIALS and METHODS: 87 cases with sepsis were investigated among 900 newborns followed in “Adnan Menderes University” NICU between January 2004-June 2008. Clinical symptoms and signs, risk factors, laboratory results, positive cultures and antibiotic sensitivity factors were evaluated in 45 cases with positive culture. RESULTS: Sepsis was found to have a 9.6% rate. 45 cases were diagnosed as definite sepsis with 49 positive blood cultures. 53% were males and 82% were premature. Most common pathogens were coagulase-negative staphylococci (CNS) and Staphylococcus aureus (S. aureus) in early neonatal sepsis, whereas CNS and the Candida in late neonatal sepsis. Mortality rate was 13.3%. Glycopeptide sensitivity of CNS was 100% , penicilline and meticilline resistance were 96% and 90% respectively. All S. aureus strains were sensitive to methicillin, clindamycine and eritromycine while penicillin resistance rate was 71.4%. No resistance was detected in Enterococcus spp. strains. Enterobacter spp. were 100% sensitive to 3. generation cephalosporins and carbapenems but resistant to ampicillin-sulbactam. Acinetobacter spp and Pseudomonas spp were resistant to piperaciline. All Klebsiella spp were sensitive to carbapenems, and aminoglycosides. No resistance was detected for the Serratia spp, Escherichia coli ve Enterococcus spp strains. CONCLUSION: Gram positive microorganisms as the leading etiologic agent in early neonatal sepsis, and their high penicillin resistance necessitated a revision in our ampiric antibiotic practices. However, no meticilline resistance was found in S. aureus species. High piperacilline resistance of Acinetobacter spp and Pseudomonas spp was associated with the recent frequent utilization of this agent

Impact of Nosocomial COVID-19 Infection Among Hospitalized Patients with Respiratory Diseases

Objective: There are very few studies concerning the frequency and course of nosocomial Coronavirus disease-2019 (COVID-19) infection among patients hospitalized having diseases other than COVID-19. In our study, patients who were admitted to the pulmonology inpatient clinic from the emergency room due to non-COVID-19 diseases and later diagnosed with COVID-19 (index cases) and the nosocomial transmission caused by these patients and the clinical outcomes were analyzed. Method: This study was carried out on 44 inpatients without COVID-19 at a pulmonology inpatient clinic during the first wave of COVID-19 pandemic. Oro-nasopharyngeal swab samples were taken at the time of hospitalization to detect COVID-19 by reverse transcription-polymerase chain reaction (RT-PCR) test. The test results of four patients were found to be positive. Due to the risk of nosocomial transmission, the remaining patients were re-evaluated for COVID-19 disease by clinical, radiological, and RT-PCR tests (1 to 3 times, and/or if symptoms developed). All patients were followed up for 30 days after discharge. Results: Thirty-six males (81%) and 8 females (19%) with a mean age of 65.6±13.6 (31-93) years were included in the study. Twenty-five of these patients had cancer, six had chronic obstructive pulmonary disease exacerbation, four had an aggravation of idiopathic pulmonary fibrosis, three had infected bronchiectasis, two had pulmonary embolisms, and four had other disorders. The RT-PCR test results were found positive in 4 patients. In about two weeks, COVID-19 infection emerged in 16 of the remaining 40 patients, and 10 of them (63% of the infected) died. The RT-PCR test results of patients with COVID-19 infection were found to be positive on day 8.2 averagely (6-13). Conclusion: Nosocomial transmission of COVID-19 may create a risk of severe illness and death among vulnerable patients. It is crucial to take necessary measures in order to reduce the risk of COVID-19 transmission in hospitals

TURKISH VERSION OF BODY AWARENESS QUESTIONNAIRE: VALIDITY AND RELIABILITY STUDY

Purpose: The Body Awareness Questionnaire (BAQ) was described as a tool with psychometric properties that thoroughly assessed the concept of body awareness. There is no Turkish version of the scale with validity and reliability. The study aimed to demonstrate the validity and reliability of the Turkish version of the BAQ. Methods: The study sample consisted of 180 university students (age=21.87 +/- 2.36 years, 99 M, 81 F). The BAQ, Self-Consciousness Scale (SCS), and Body Cathexis Scale (BCS) were applied to the participants. Results: Result of the correlation analysis between the BAQ and the total scores obtained from the SCS and BCS, and the correlation coefficients were determined as 0.802 (p=0.007) and -0.753 (p=0.009), respectively. As a result of the explanatory factor analysis, a measurement tool consisting of 18 items and four sub-groups explaining 66% of the variance was obtained. The test retest reliability coefficient was 0.830 at 3-day intervals. Cronbach's alpha was calculated as 0.917 to determine internal consistency. Conclusion: Findings in the study show that the Turkish version of BAQ is valid and reliable

Koah'lı hastalarda vücut farkındalığı tedavisinin denge ve düşme korkusuna etkisi: Pilot çalışma

GİRİŞ-AMAÇ: Kronik akciğer hastalıkları ile ilgili tedavi kılavuzlarında akciğer dışı sistemlerin ve semptomların da değerlendirilmesi önerilmektedir. Bu kapsamda özellikle son dönemde Kronik Obstrüktif Akciğer Hastalığı(KOAH)’lı hastalarda denge ve düşmenin etkilenimine dair yapılan çalışmaların sayısı artmış olup; bu araştırmalar KOAH’lı hastaların hastalığın şiddeti ile orantılı şekilde; fonksiyonel denge kısıtlılığı ve düşme korkusuna sahip olduklarını göstermiştir. KOAH’da pulmoner rehabilitasyonun (PR) etkinliği iyi tanımlanmış olsa da, postüral kontrol, denge ve düşme korkusu düzeyinde minimal kazanım sağlamaktadır. KOAH’ lılarda denge ve düşmeyi iyileştirecek alternatif tedavilere ihtiyaç duyulmaktadır. Literatüre bakıldığında Vücut Farkındalığı Tedavisi (VFT)’ nin çeşitli hasta gruplarında yapılan egzersizin kalitesini, koordinasyonu, dengeyi, postüral kontrolü, yaşam kalitesini, zihin-vücut bütünleşmesini arttırdığı görülmektedir. Bu pilot çalışma ile KOAH’lı hastalarda PR programına ek olarak verilen VFT’nin denge ve düşme korkusu üzerindeki etkisini araştırmak amaçlanmıştır. YÖNTEM: Deneysel bir çalışma olarak katılımcılar deney(n=11) ve kontrol(n=11) grubuna ayrıldı. Deney ve kontrol grubundaki tüm hastalara 8 hafta boyunca, haftanın her günü 30 dakikalık PR programı verildi. Deney grubundaki hastalara PR programına ek olarak 8 hafta boyunca, VFT haftada 1 gün, günde 1 saat uygulandı. Katılımcıların statik ve dinamik dengesi Balance Master System denge ve performans test cihazı ile düşme korkusu ise Düşme Etkinlik Ölçeği (DEÖ) ile değerlendirildi. Sekiz haftanın sonunda başlangıçta yapılan tüm değerlendirmeler tekrarlandı. BULGULAR: İki grupta da başlangıca göre dengede gelişme ve düşme korkusunda azalma saptandı. Her iki tedavi etkili bulundu; gruplar arası karşılaştırmada dinamik denge ve düşme korkusu seviyesi VFT grubu lehine belirgin düzeyde farklı iken (p0,05). SONUÇ: KOAH’lı hastalarda VFT’nin denge ve düşme korkusunun iyileştirilmesinde PR’ye göre daha etkili olduğu saptanmıştır. Fiziksel bilinci geliştirerek doğru vücut uyumunu ve mekaniğini kurmayı hedefleyen VFT, KOAH’lı hastalarda dengeyi geliştirmek ve düşme korkusunu azaltmak için etkili bir alternatif yöntemdir; KOAH hastaları arasında başa çıkma becerilerini ve bilişsel davranışları geliştirmenin yanı sıra daha iyi hareket etmelerine yardımcı olabileceği ve böylece günlük yaşam aktivitelerini gerçekleştirme yeteneklerini geliştirebileceği için pulmon

Internipple Distance and Internipple Index in Prepubertal Turkish Girls

Objective: To determine internipple distance and internipple index in prepubertal Turkish girls.Methods: The internipple distance and chest circumference of 667 healthy prepubertal Turkish girls aged 6 to 11 years were measuredin a school screening program in Düzce. Measurements were performed at the end of expiration with a standard non-stretch tapemeasure graduated in millimeters with the arms hanging in a relaxed position on the sides of the body. The internipple distance wasmeasured between the centers of both nipples, and chest circumference was measured across the internipple line. The internipple indexwas calculated by dividing the internipple distance (cm) x100 by the chest circumference (cm). Age specific internipple index referencecurves were constructed and smoothed with the Lambda-Mu-Sigma method. Mean and standard deviations of internipple distance andinternipple index were calculated according to decimal ages.Results: Age was found to be positively correlated with internipple distance and chest circumference, while it was negatively correlatedwith internipple index. The reference values of internipple index, including 3rd, 10th, 25th, 50th, 75th, 90th, and 97th percentiles, andstandard deviations were calculated for prepubertal girls.Conclusion: The reference ranges provided by this study might be helpful for the evaluation of syndromic cases by serving as normativedata for internipple index in prepubertal girls aged 6-11 years in Turkey although ethnic differences may affect applicability to othercountries.WOS:0005658661000072-s2.0-8509009382