Design of FPGA-Implemented Reed-Solomon Erasure Code (RS-EC) Decoders With Fault Detection and Location on User Memory

Reed–Solomon erasure codes (RS-ECs) are widely used in packet communication and storage systems to recover erasures. When the RS-EC decoder is implemented on a field-programmable gate array (FPGA) in a space platform, it will suffer single-event upsets (SEUs) that can cause failures. In this article, the reliability of an RS-EC decoder implemented on an FPGA when there are errors in the user memory is first studied. Then, a fault detection and location scheme is proposed based on partial reencoding for the faults in the user memory of the RS-EC decoder. Furthermore, check bits are added in the generator matrix to improve the fault location performance. The theoretical analysis shows that the scheme could detect most faults with small missing and false detection probability. Experimental results on a case study show that more than 90% of the faults on user memory could be tolerated by the decoder, and all the other faults can be detected by the fault detection scheme when the number of erasures is smaller than the correction capability of the code. Although false alarms exist (with probability smaller than 4%), they can be used to avoid fault accumulation. Finally, the fault location scheme could accurately locate all the faults. The theoretical estimates are very close to the experiment results, which verifies the correctness of the analysis done.This work was supported in part by the National Natural Science Foundation of China under Grant 61501321, in part by the China Postdoctoral Science Foundation and Luoyang Newvid Technology Company, Ltd., and in part by the ACHILLES Project PID2019-104207RB-I00 funded by the Spanish Ministry of Science and Innovation

Adsorption–desorption behavior of malachite green by potassium permanganate pre-oxidation polyvinyl chloride microplastics

Microplastics (MPs) and the typical hydrophilic organic pollutant Malachite green (MG) are frequently detected in sewage treatment plants. Potassium permanganate (KMnO4) pre-oxidation is an economical and effective technology in wastewater treatment. It is important to study the surface physicochemical characteristics of MPs and understand their fate in wastewater treatment plants after pre-oxidation. In this study, Polyvinyl chloride (PVC) MPs were treated by single and composite KMnO4 pre-oxidation with different pH values. After the pre-oxidation treatment, the appearance of Osingle bondMn spectra and surface nanoparticles indicated the oxides (MnO2) were produced on the MPs surface. Moreover, the adhesion of MnO2 is helpful to improve the hydrophilicity and adsorption capacity of MG. The adsorption capacity of pristine PVC for MG was 2.6 mg/g. But the adsorption capacity increased to 7.0 mg/g for single oxidation and 140.7 mg/g for composite oxidation, respectively. The desorption experiment results indicate the pre-oxidation process could reduce the release efficiency of MG from the PVC MPs due to the better binding of surface MnO2 nanoparticles to MG. However, the total desorption capacity is still high. which illustrates that there is a high potential risk of MG which can transfer from the surface of the PVC MPs to the gastrointestinal fluids.publishedVersio

Competition adsorption of malachite green and rhodamine B on polyethylene and polyvinyl chloride microplastics in aqueous environment

Microplastics (MPs) will cause compound pollution by combining with organic pollutants in the aqueous environment. It is important for environmental protection to study the adsorption mechanism of different MPs for pollutants. In this study, the adsorption behaviors of malachite green (MG) and rhodamine B (RhB) on polyethylene (PE) and polyvinyl chloride (PVC) were studied in single systems and binary systems, separately. The results show that in single system, the adsorptions of between MPs for pollutants (MG and RhB) are more consistent with the pseudo-second-order kinetics and Freundlich isotherm model, the adsorption capacity of both MPs for MG is greater than that of RhB. The adsorption capacities of MG and RhB were 7.68 mg/g and 2.83 mg/g for PVC, 4.52 mg/g and 1.27 mg/g for PE. In the binary system, there exist competitive adsorption between MG and RhB on MPs. And the adsorption capacities of PVC for the two dyes are stronger than those of PE. This is attributed to the strong halogen-hydrogen bond between the two dyes and PVC, and the larger specific surface area of PVC. This study revealed the interaction and competitive adsorption mechanism between binary dyes and MPs, which is of great significance for understanding the interactions between dyes and MPs in the multi-component systems.publishedVersio

Desorption of sulfamethoxazole from polyamide 6 microplastics: Environmental factors, simulated gastrointestinal fluids, and desorption mechanisms

Microplastics (MPs) can enrich pollutants after being released into the environment, and the contaminants-loaded MPs are usually ingested by organisms, resulting in a potential dual biotoxic effect. In this paper, the adsorption behavior of Sulfamethoxazole (SMX) on Polyamide 6 (PA6) MPs was systematically investigated and simulated by the kinetic and isotherm models. The effect of environmental conditions (pH, salinity) on the adsorption process was studied, and the desorption behavior of SMX-loaded PA6 MPs was focused on simulating the seawater, ultrapure water, gastric and intestinal fluids. We found that lower pH and solubilization of SMX by gastrointestinal components (bovine serum albumin (BSA), sodium taurocholate (NaT), and pepsin) can reduce the electrostatic interaction between the surface charge of PA6 MPs and SMX. The result will lead to an increase in the desorption capacity of SMX-loaded PA6 MPs in gastrointestinal fluids and therefore will provide a reasonable mechanism for the desorption of SMX-loaded PA6 MPs in the gastrointestinal fluids. This study will provide a theoretical reference for studying the desorption behavior of SMX-loaded PA6 MPs under gastrointestinal conditions.publishedVersio

Comparing the adsorption of methyl orange and malachite green on similar yet distinct polyamide microplastics: Uncovering hydrogen bond interactions

Microplastics (MPs) and dye pollutants are widespread in aquatic environments. Here, the adsorption characteristics of anionic dye methyl orange (MO) and cationic dye malachite green (MG) on polyamide 6 (PA6) and polyamide 66 (PA66) MPs were investigated, including kinetics, isotherm equilibrium and thermodynamics. The co-adsorption of MO and MG under different pH was also evaluated. The results reveal that the adsorption process of MO and MG is suitably expounded by a pseudo-second-order kinetic model. The process can be characterized by two stages: internal diffusion and external diffusion. The isothermal adsorption equilibrium of MO and MG can be effectively described using the Langmuir model, signifying monolayer adsorption. Furthermore, the thermodynamic results indicated that the adsorption was spontaneous with exothermic and endothermic properties, respectively. The results of binary systems reveal that MO dominates the adsorption at low pH (2–5), while MG dominates at high pH (8–10). Strong competitive adsorption was observed between MO and MG in neutral conditions (pH 6–8). The desorption experiments confirm that PA6 and PA66 could serve as potential carriers of MO and MG. The interaction between dyes and polyamide MPs is primarily mediated through hydrogen bonds and electrostatic attraction. The results reveal that PA6 formed more hydrogen bonds with the dyes, resulting in higher adsorption capacity than that of PA66. This difference can be attributed to the disparities in the synthesis process and polymerization method. Our study uncovered the adsorption mechanism of dye pollutants on PA6 and PA66, and provided a more comprehensive theoretical basis for the risk assessment concerning different types of polyamide MPs in aquatic environments.publishedVersio

Establishment and Characterization of an Immortalized Porcine Oral Mucosal Epithelial Cell Line as a Cytopathogenic Model for Porcine Circovirus 2 Infection

Porcine circovirus 2 (PCV2) is a major etiological agent for porcine circovirus-associated diseases and causes enormous economic losses in domestic and overseas swine production. However, there are currently no suitable cell models to study the cytopathic effects (CPE) of PCV2 in vitro, which severely restricts the study of PCV2 pathogenesis. In the present study, we established an immortalized porcine oral mucosal epithelial cell line (hTERT-POMEC) by introducing the hTERT gene into primary porcine oral mucosal epithelial cells (POMECs) derived from a neonatal, unsuckled piglet. The hTERT-POMEC cells have a homogeneous cobblestone-like morphology and retain the basic physiological properties of primary POMECs. No chromosome abnormality and tumorigenicity transformation was observed in immortalized hTERT-POMECs. Viral infection assays demonstrated that PCV2 propagated and caused CPE in hTERT-POMECs. We conclude that the immortalized cell line hTERT-POMEC is a crucial tool for further research into the pathogenesis of PCV2

Design of a novel 3D tip-based nanofabrication system with high precision depth control capability

The design, analysis, and experimental investigation of a novel 3D tip-based nanofabrication system with high precision depth control capability is presented in this paper. Based on this system, a new depth control method, namely tip displacement-based closed-loop (DC) depth control methodology is proposed to improve the depth control capability. As the force-depth prediction with the commonly-used depth control method, i.e. the normal force-based closed-loop (FC) method, may depend on the machining speed, the machining direction, and the material properties, etc. Compared with the FC method, the DC method decreases the complexity and the high uncertainty. The tip feed system utilizes a non-contact force, i.e. the electromagnetic force, to adjust the tip displacement. Therefore, the tip support mechanism can be used to accomplish the tip-sample contact detection. Additionally, an active compensation method is proposed to eliminate the tilt angle between the sample surface and the horizontal plane. Otherwise the machining depth will change gradually, i.e. getting deeper or lower. Furthermore, a series of patterns have been fabricated on silicon sample surface with the proposed system and method. The maximum machining depth of a single scan reaches 300 nm, which is much larger than that of an atomic force microscope (AFM)-based nanofabrication system. The experimental results demonstrate that the system has advantages of distinguished depth control capability, high machining accuracy, and excellent repeatability, which diminishes the influence of above-mentioned factors on the machining depth. Also, the method has the potential of machining arbitrary 2D/3D patterns with well-controlled depth and high accuracy

Neutral operator with variable parameter and third-order neutral differential equation

Additional file 1. The TRS sequences of structural genes in the HP-PRRSV/SD16 genome (GenBank: JX087437)

Detection of the dominant pathogens in diarrheal calves of Ningxia, China in 2021–2022

IntroductionCalf diarrhea is a complex disease that has long been an unsolved problem in the cattle industry. Ningxia is at the forefront of China in the scale of cattle breeding, and calf diarrhea gravely restricts the development of Ningxia's cattle industry.MethodsFrom July 2021 to May 2022, we collected diarrhea stool samples from calves aged 1–103 days from 23 farms in five cities in Ningxia, and performed PCR using specific primers for 15 major reported pathogens of calf diarrhea, including bacteria, viruses, and parasites. The effect of different seasons on the occurrence of diarrhea in calves was explored, the respective epidemic pathogens in different seasons were screened, and more detailed epidemiological investigations were carried out in Yinchuan and Wuzhong. In addition, we analyzed the relationship between different ages, river distributions and pathogen prevalence.ResultsEventually, 10 pathogens were detected, of which 9 pathogens were pathogenic and 1 pathogen was non-pathogenic. The pathogens with the highest detection rate were Cryptosporidium (50.46%), Bovine rotavirus (BRV) (23.18%), Escherichia coli (E. coli) K99 (20.00%), and Bovine coronavirus (BCoV) (11.82%). The remaining pathogens such as Coccidia (6.90%), Bovine Astrovirus (BoAstV) (5.46%), Bovine Torovirus (BToV) (4.09%), and Bovine Kobuvirus (BKoV) (3.18%) primarily existed in the form of mixed infection.DiscussionThe analysis showed that different cities in Ningxia have different pathogens responsible for diarrhea, with Cryptosporidium and BRV being the most important pathogens responsible for diarrhea in calves in all cities. Control measures against those pathogens should be enforced to effectively prevent diarrhea in calves in China