33 research outputs found
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There are many kind of carbohydrate compound having strong bioactivity. Authors had been reported that natural polysaccharide and olligosaccharide would have novel biological activity by chemical modification. Structure analysis of chemically modified dietary fiber polygalactosamine having NH_2 group on 2nd position by use of molecular force field calculation wsa also reported. In this paper, we described that synthesis and structure analysis of sulfated polysaccharides, such as polygalactosamin and chitosan. Molecurar orbital and semi-empirical molecular orbital methods were used for 3-dimentional graphical mapping of electron density, electrostatic potential (the energy of interaction of a point positive charge with the nuclei of a molecule), and local ionaization potential (a measure of the ease with which electrons are detached from a region of space)
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The presence of modified genes resistant to a chemical herbicide Roundup Ready (glyphosate) in commercially available bean curd (tofu) labeled as modified gene-free was examined by the PCR method. Samples were taken from twelve brands of bean curd all available on the market and bought during August and September 2005. For DNA extraction, PCR analysis was conducted from primers based on the disclosed Roundup Ready-resistant expression sequence and soybean lectin expression sequence using a DNeasy Plant Mini Kit produced by Qiagen, and the results were confirmed by electrophoresis. As a result a clear band that appears to derive from modified genes was detected in one brand, and a trace of such a band was detected in two other brands. In an analysis of unsorted ration for livestock carried out for comparison, a clear band that appears to derive from modified genes was detected
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žéãšããŠææ¡ãããIn order to lower a false positive risk in biomonitoring workers exposed to toluene, the relationship between intake of benzoic acid (BA) contained in foods and urinary hippuric acid (HA) concentration was studied. Five male and female adults were administered placebo drink which contained 100mg BA. The urinary HA/creatinine concentration before, 30 and 60 minutes after the administration were 0.18±0.06, 1.82±0.33 and 1.68±0.49g/g, respectively. The urinary concentration ratio after 90 minutes was rapidly decreased, and after 180 minutes, it exhibited a value close to the basal level. The HA concentrations found in experiment in which subjects ate the dried fruit cranberry (containing 22mg BA) were lower than that of placebo groups, but the excretion pattern was similar to each other. Gotoh (2002) reported the excretion of urinary HA when taking 137, 68.5 and 50mg BA-containing soft drink. HA excretion patterns found in this report and that of reported in Gotoh were very similar, and showed a dose-response relationships between BA intake and urinary HA concentration. These results may suggest that the food derived HA can be estimated, if the amount of intaken BA from foods is determined
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Quantification of how much a kimono loses its shape when worn is a fundamental key factor in studying the correlation between a kimono design and its loss of shape. The establishment of a precise method for the quantification of the degree of shape loss has been pursued in this study. To obtain the changing positions of a kimono on the body by visual method, a check patterned yukata was selected and sewn for the test. The position of the yukata on parts of the body before and after certain movements made by the wearer were recorded, using a high-resolution digital camera. Movements involved walking up and down a staircase, and raising and lowering both arms. FulHength images of the wearer were taken from the front, back, right side and left side, with he/she standing in front of a 10cm grid screen. Using Adobe Photoshop as an image analysis software, the checkered patterns of the yukata were isolated from each image, and overlaid on the corresponding image which was made semi-transparent. For the overlay, the toes or heels were used as registration points for the lower end, and the base of the neck between the collarbones for the top end, while the latter was marked as a fixed reference point. This was found to be an ideal registration point as it was completely free from the effects of the head movement. Distances moved were measured on the overlaid images, at the center, collar, obi, ohashori and hem points. This method enabled an accurate quantification of the distances moved of each part. Although it was possible in the case of the front images to successfully fix the body position by selecting the base of the neck between the collarbones and the toes as registration points, it was suggested that the images taken from the other directions may cause some deviation depending on the head position, as the top of the head was selected as a registration point. This study will serve as an important guideline for the improvement of kimono design and dressing techniques
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A positively charged polylysine dendrimer generation 3 (LDG3) produced gels by interacting with a negatively charged curdlan sulfate which is a sulfated polysaccharide with high anti-AIDS virus activity. 1H NMR spectra showed a new peak caused by gel formation, which became highest at the ion ratio of 0.75. In addition, when a linear polyornithine (PO) were mixed with curdlan sulfate, gel formation also occurred. In the molar ratio of 0.7, the mixture afforded 100% gel. This result suggests that the action mechanism of curdlan sulfate is attributed to an ionic interaction