Serrated Lesions of Gastrointestinal Tract and Significance of Serrated Changes in Mucosa of Inflammatory Bowel Disease Patients

Souhrn Pacienti s idiopatickým střevním zánětem mají zvýšené riziko vzniku kolorektálního karcinomu. IBD-asociovaná dysplázie je považována za konvenční prekurzor pro IBD- asociovaný adenokarcinom. (1-2) U pacientů s IBD jsou však popisovány i jiné nekonvenční slizniční změny s pilovitou a vilózní hypermucinózní morfologií, ale jejich preneoplastický potenciál není zcela objasněn. (3-4) Cílem studie byla retrospektivní revize vzorků od pacientů s IBD se zaměřením na detekci různých typů slizničních změn včetně nekonvenčních lézí a zhodnocení jejich vztahu a imunohistochemických a molekulárních vlastností. Revize zahrnovala chirurgické anebo endoskopické vzorky od pacientů s diagnózou IBD vyšetřené v průběhu 10leté periody. Zastižené slizniční léze byly rozděleny do 3 skupin. Do skupiny 1 byly zařazeny nekonvenční léze (PPL), do skupiny 2 pravé pilovité polypy a do skupiny 3 IBD-dysplázie a kolorektální karcinom. Detailní morfologické hodnocení, imunohistochemické vyšetření exprese mismatch repair proteinů a/nebo MLH1, p53 a O6 -methylguanine DNA metyltransferázy (MGMT) a molekulární vyšetření mutačního stavu genů KRAS, NRAS a BRAF bylo provedeno ve všech detekovaných lézích. V rámci studie byly revidovány vzorky celkem od 309 pacientů. U 51 pacientů jsme zastihli 88 slizničních lézí. Nejčastější byly léze...Patients with inflammatory bowel disease (IBD) have an elevated risk of developing colorectal carcinoma (CRC). IBD-dysplasia is regarded as a conventional precursor for IBD- associated carcinoma. (1-2) Other types of non-conventional mucosal changes with serrated and villous hypermucinous morphology have also been reported but their preneoplastic potential is still not well elucidated. (3-4) The aim of the study was to retrospectively review samples from IBD patients focusing on detection of mucosal lesions including non-conventional lesions and evaluate their relationship and immunohistochemical and molecular properties. Surgical specimens and/or endoscopical biopsy samples of IBD patients examined during a 10-year period were reviewed. Mucosal lesions were divided into three groups - group 1 of non-conventional lesions, group 2 of true serrated polyps, and group 3 of IBD-dysplasia and colorectal carcinoma. Detailed morphological evaluation, immunohistochemical analysis of mismatch repair proteins and/or MLH1, p53 and O6 -methylguanine DNA methyltransferase (MGMT) expression, and molecular analysis for KRAS, NRAS and BRAF gene mutation was performed in all lesions. Overall, samples from 309 IBD patients were reviewed. A total of 88 mucosal lesions were found in 51 patients. Most common were lesions...Fingerlandův ústav patologieThe Fingerland Department of PathologyLékařská fakulta v Hradci KrálovéFaculty of Medicine in Hradec Králov

Effectiveness of activities with clients of low-threshold clubs from the point of view of social workers

HTF - Katedra učitelstvíHusitská teologická fakultaHussite Theological Facult

Students' knowledge of selected basic schools about physical abuse of a child in the family

Katedra pedagogikyPedagogická fakultaFaculty of Educatio

Rare myxoid liposarcoma metastasis to the interventricular septum of the heart

AbstractLiposarcomas are malignant tumors of the soft tissue. Myxoid liposarcoma is the second most common subtype of these tumors in adults. It accounts for approximately 20% of all malignant soft tissue tumors [1,2]. Peak of its incidence occurs between 40 to 60 years of age with relatively indolent clinical course Matsumoto et al. (2007) [3], Cho et al. (2010) [4], Faiman et al. (2005) [5]. Typical localizations of myxoid liposarcoma comprise limbs, particularly thighs with a tendency to metastasize into extrapulmonary sites such as retroperitoneum, mediastinum, bones. Cardiac metastases are extremely rare.We present a case of a 36-year-old man with a history of recurrent myxoid liposarcoma. Primary location was in the left popliteal area. After extirpation of the tumor, metastatic tumor was subsequently revealed in the right axilla. Each surgical extirpation was followed by radiation therapy and brachytherapy. Cardiac metastasis was accidentally diagnosed with PET/CT during the staging process. The patient was asymptomatic and was admitted to our institution for further diagnostics and treatment. After confirmation of its location, the tumor was excised. Histological examination revealed myxoid liposarcoma

Students' knowledge of selected basic schools about physical abuse of a child in the family

Katedra pedagogikyPedagogická fakultaFaculty of Educatio

Typ kladného a záporného hrdiny v povídkách, románech, umělecko-naučné literatuře a životopisných knihách pro mládež ve věku od 9 do 15 let, vydávaných v Československu od roku 1949 do roku 1968

Katedra psychologieFilozofická fakult

Analysis of conditions of a usage resources from the fund EAFRD in FC Hluboké Mašůvky

This thesis concentrates on pumping and a usage of the EAFRD resources, on conditions that are the applicants asked to fulfil in order to use these dotation resources. A practical example is a compilation of a project for the agricultural cooperation Hluboké Mašůvky

Serrated Lesions of Gastrointestinal Tract and Significance of Serrated Changes in Mucosa of Inflammatory Bowel Disease Patients

Patients with inflammatory bowel disease (IBD) have an elevated risk of developing colorectal carcinoma (CRC). IBD-dysplasia is regarded as a conventional precursor for IBD- associated carcinoma. (1-2) Other types of non-conventional mucosal changes with serrated and villous hypermucinous morphology have also been reported but their preneoplastic potential is still not well elucidated. (3-4) The aim of the study was to retrospectively review samples from IBD patients focusing on detection of mucosal lesions including non-conventional lesions and evaluate their relationship and immunohistochemical and molecular properties. Surgical specimens and/or endoscopical biopsy samples of IBD patients examined during a 10-year period were reviewed. Mucosal lesions were divided into three groups - group 1 of non-conventional lesions, group 2 of true serrated polyps, and group 3 of IBD-dysplasia and colorectal carcinoma. Detailed morphological evaluation, immunohistochemical analysis of mismatch repair proteins and/or MLH1, p53 and O6 -methylguanine DNA methyltransferase (MGMT) expression, and molecular analysis for KRAS, NRAS and BRAF gene mutation was performed in all lesions. Overall, samples from 309 IBD patients were reviewed. A total of 88 mucosal lesions were found in 51 patients. Most common were lesions..