Protective effect of Garcinia against renal oxidative stress and biomarkers induced by high fat and sucrose diet

<p>Abstract</p> <p>Background</p> <p>Obesity became major health problem in the world, the objective of this work was to examine the effect of high sucrose and high fat diet to induce obesity on antioxidant defense system, biochemical changes in blood and tissue of control, non treated and treated groups by administration of Garcinia cambogia, and explore the mechanisms that link obesity with altered renal function</p> <p>Methods</p> <p>Rats were fed a standard control diet for 12 week (wk) or a diet containing 65% high sucrose (HSD) or 35% fat (HFD) for 8 wk and then HFD group divided into two groups for the following 4 wks. One group was given <it>Garcinia</it>+HFD, the second only high fat, Also the HSD divided into two groups, 1^stHSD+<it>Garcinia </it>and 2^ndHSD. Blood and renal, mesenteric, Perirenal and epididymal adipose tissues were collected for biochemical assays.</p> <p>Results</p> <p>HFD and HSD groups of rats showed a significant increase in feed intake, Body weight (BW) and body mass index (BMI). Also there were significant increases in weights of mesenteric, Perirenal and epididymal adipose tissues in HFD and HSD groups.</p> <p>HFD and HSD affect the kidney by increasing serum urea and creatinine levels and decreased level of nitric oxide (NO) and increased blood glucose, low density lipoproteins (LDL), triacylglycerol (TG), total cholesterol (TC) and malondialdehyde (MDA). Glucose 6-phosphate dehydrogenase (G6PD) activities were significantly decreased in HFD while there were significant increases in HSD and HSD+G groups p ≤ 0.05 compared with control. Moreover, renal catalase activities and MDA levels were significantly increased while NO level was lowered. These changes improved by <it>Garcinia </it>that decreased the oxidative stress biomarkers and increased NO level.</p> <p>There were significant positive correlations among BMI, kidney functions (Creatinine and urea), TG and Oxidative markers (renal MDA and catalase).</p> <p>Conclusions</p> <p>Rats fed a diet with HFD or HSD showed, hypertriglyceridemia, increased LDL production, increased oxidative stress and renal alteration. Moreover, suggesting association between lipid peroxidation, obesity and nephropathy, while <it>Garcinia </it>ameliorated the damaging effects of the HFD or HSD and decreased feed intake, MDA level and decreased oxidative stress in renal tissues.</p

The relation of high fat diet, metabolic disturbances and brain oxidative dysfunction: modulation by hydroxy citric acid

<p>Abstract</p> <p>Aims</p> <p>This study aimed to examine the effect of high fat diet (HFD) to modulate brain dysfunction, and understand the linkages between obesity, metabolic disturbances and the brain oxidative stress (BOS) dysfunction and modulation with hydroxyl citric acid of <it>G. Cambogia</it>.</p> <p>Methods</p> <p>Rats were divided into 3 groups; 1^stcontrol, maintained on standard normal rat chow diet, 2^ndHFD, maintained on high fat diet along 12 week and 3^rdHFD+G, administered <it>G. Cambogia </it>for 4 weeks and each group include 8 rats. Blood, brain and abdominal fat were collected for biochemical measurements.</p> <p>Results</p> <p>HFD group showed significant increase in energy intake, final BW and BW gain. Also significant increase in weight of abdominal fat in HFD group. HFD induce metabolic disturbance through increasing the lipid profile (LDL, TG, TC), γGT and α-amylase activity, uric acid level and hyperglycemia, while decreasing creatine kinase (CK) activity.</p> <p>These changes associated with lowering in brain nitric oxide (NO) level and rising in serum butyrylcholinesterase (BChE), brain catalase activity and MDA levels as oxidative stress markers. These alterations improved by <it>G. Cambogia </it>that decrease BOS and increased NO level.</p> <p>Conclusions</p> <p>Rats fed HFD showed, metabolic disturbances produce hyperglycemia, hypertriglyceridemia, hypercholesterolemia and increased LDL associated with increased BOS. Involvement of BuChE, NO and oxidative stress associated with metabolic disturbances in the pathophysiological progression in brain, suggesting association between obesity, metabolic disorders and brain alteration while, using <it>G. Cambogia</it>, ameliorate the damaging effects of the HFD via lowering feed intake and BOS.</p

Community Structure and Spatial Distribution of Fish Juvenile in Mangrove and Seagrass Habitats in Pramuka Island

This study was aimed to investigate the community structure of fish juvenile, spatial distribution and similarity from both adjacent habitats of mangrove and seagrass. This study was conducted in the eastern part of Pramuka island from April to June 2015. The samples were collected by using line transect method in three observation areas that were spatially connected i.e., mangrove, transition and seagrass zones. The result of the study from three observation zones revealed that there were found 24 species of fishes from 15 families i.e., Siganidae (4 species), Apogonidae (3 species), Ger-reidae (2 species), Terapontidae (2 species), Gobiidae (2 species), Labridae (2 species), Mugilidae, Nemipteridae, Hemiramphidae, Sphyraenidae, Monacanthidae, Atherinidae, Pomacentridae, Lut-janidae, and Lethrinidae (each of them 1 species). According to community structure, the adjacent ob-servation zones did not show a significant difference in the number of species, abundance, and bio-mass. According to fish distribution, fish species in transition zone and seagrass zone were relatively similar and dominated by Gerres oblongus, Fibramia lateralis, and Siganus canaliculatus. Mean-while, in mangrove zone revealed a significant different of fish species than in transition and seagrass zones. In mangrove zone, fish species was dominated by Gerres oblongus and Siganus guttatus

The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline

Kamal A Amin1, Mohamed S Hassan2, El-Said T Awad3, Khalid S Hashem11Department of Biochemistry, 2Department of Internal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt; 3Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Cairo, EgyptObjective: The objective of the present study was to determine the ability of cerium oxide (CeO2) nanoparticles to protect against monocrotaline (MCT)-induced hepatotoxicity in a rat model.Method: Twenty male Sprague Dawley rats were arbitrarily assigned to four groups: control (received saline), CeO2 (given 0.0001 nmol/kg intraperitoneally [IP]), MCT (given 10 mg/kg body weight IP as a single dose), and MCT + CeO2 (received CeO2 both before and after MCT). Electron microscopic imaging of the rat livers was carried out, and hepatic total glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) enzymatic activities were quantified.Results: Results showed a significant MCT-induced decrease in total hepatic GSH, GPX, GR, and GST normalized to control values with concurrent CeO2 administration. In addition, MCT produced significant increases in hepatic CAT and SOD activities, which also ameliorated with CeO2.Conclusions: These results indicate that CeO2 acts as a putative novel and effective hepatoprotective agent against MCT-induced hepatotoxicity.Keywords: monocrotaline, ceruim oxide nanoparticle, hepatotoxicity, oxidative stres

Improving irrigation water delivery performance of a large-scale rice irrigation scheme

The availability of irrigation water and its equitable allocation in a large-scale rice irrigation scheme of Malaysia have been modeled. The model reliably estimates available water for irrigation at the intake of the main canal and simulates the recommended irrigation deliveries for 120 tertiary canals. Different water allocation and management scenarios were evaluated based on the sensitivity of the growth stages of rice to water, varying field-water demand, and perceived water shortages. The model provides a quantitative assessment not only of water allocation for irrigation but also of day-to-day or periodic irrigation delivery performances for a large-scale rice irrigation system. It provides 86% adequacy and 87% equity of irrigation delivery in the main season (August-December). The corresponding performance indicators provided by the model are 74 and 89% in the off-season (February-June). The dependability of water supply is higher in the off-season than in the main season, while the relative water supply (RWS) is the converse. RWS often becomes >1.0 in the main season, while such a RWS is rarely obtained in the off-season. The model augments the water delivery performance of the scheme and hence would serve as a useful tool for irrigation managers in decision making

Optical and Optoelectronic Studies of Binary and Ternary Films of Poly(L-Tryptophane), Poly(5-hydroxy-L-tryptophane), and P(TER-CO-TRI) Doped with Sudan Dye

In this work, the optical properties and optoelectronics parameters of binary and ternary composite films made of two electron acceptors, poly(L-Tryptophane) and poly(5-hydroxy-L-Tryptophane), with an electron donor, P(TER-CO-TRI), doped with Sudan dyes, are comprehensively investigated. The films with different volumetric ratios of the components were deposited onto the glass substrates using spin coating technique. Results showed that with the help of dye doping into the binary systems of poly(L-Tryptophane):P(TRI-co-TER) (1:2) and poly(5-hydroxy-L-Tryptophane):P(TRI-co-TER)(1:2), the refractive index was increased from 2.01 to 2.32. The nature of the electronic transition in the studied films was found to be a direct allowed transition, which was derived from Tauc’s equation. The combination of Cyclic voltammetry (CV) technique and absorption spectroscopy was used to determine the molecular energy levels, HOMO and LUMO of the polymer samples. It was seen that the mixture of poly(L-Tryptophane):P(TRI-co-TER):Sudan dye (1:2:2) has led to increase the energy gap to 2.95 eV and the real optical conductivity ( ) to about 433.11 S.cm-1. According to the findings, the investigated polymers can have a great potential for semitransparent organic solar cells

Contamination of Fresh Produce with Antibiotic-Resistant Bacteria and Associated Risks to Human Health: A Scoping Review.

Fresh produce, when consumed raw, can be a source of exposure to antimicrobial residues, antimicrobial-resistant bacteria (ARB) and antimicrobial resistance genes (ARGs) of clinical importance. This review aims to determine: (1) the presence and abundance of antimicrobial residues, ARB and ARGs in fresh agricultural products sold in retail markets and consumed raw; (2) associated health risks in humans; and (3) pathways through which fresh produce becomes contaminated with ARB/ARGs. We searched the Ovid Medline, Web of Science and Hinari databases as well as grey literature, and identified 40 articles for inclusion. All studies investigated the occurrence of multidrug-resistant bacteria, and ten studies focused on ARGs in fresh produce, while none investigated antimicrobial residues. The most commonly observed ARB were E. coli (42.5%) followed by Klebsiella spp. (22.5%), and Salmonella spp. (20%), mainly detected on lettuce. Twenty-five articles mentioned health risks from consuming fresh produce but none quantified the risk. About half of the articles stated produce contamination occurred during pre- and post-harvest processes. Our review indicates that good agricultural and manufacturing practices, behavioural change communication and awareness-raising programs are required for all stakeholders along the food production and consumption supply chain to prevent ARB/ARG exposure through produce

Effective Treatment Strategies for the Removal of Antibiotic-Resistant Bacteria, Antibiotic-Resistance Genes, and Antibiotic Residues in the Effluent From Wastewater Treatment Plants Receiving Municipal, Hospital, and Domestic Wastewater: Protocol for a Systematic Review.

BACKGROUND: The widespread and unrestricted use of antibiotics has led to the emergence and spread of antibiotic-resistant bacteria (ARB), antibiotic-resistance genes (ARGs), and antibiotic residues in the environment. Conventional wastewater treatment plants (WWTPs) are not designed for effective and adequate removal of ARB, ARGs, and antibiotic residues, and therefore, they play an important role in the dissemination of antimicrobial resistance (AMR) in the natural environment. OBJECTIVE: We will conduct a systematic review to determine the most effective treatment strategies for the removal of ARB, ARGs, and antibiotic residues from the treated effluent disposed into the environment from WWTPs that receive municipal, hospital, and domestic discharge. METHODS: We will search the MEDLINE, EMBASE, Web of Science, World Health Organization Global Index Medicus, and ProQuest Environmental Science Collection databases for full-text peer-reviewed journal articles published between January 2001 and December 2020. We will select only articles published in the English language. We will include studies that measured (1) the presence, concentration, and removal rate of ARB/ARGs going from WWTP influent to effluent, (2) the presence, concentration, and types of antibiotics in the effluent, and (3) the possible selection of ARB in the effluent after undergoing treatment processes in WWTPs. At least two independent reviewers will extract data and perform risk of bias assessment. An acceptable or narrative synthesis method will be followed to synthesize the data and present descriptive characteristics of the included studies in a tabular form. The study has been approved by the Ethics Review Board at the International Centre for Diarrhoeal Disease Research, Bangladesh (protocol number: PR-20113). RESULTS: This protocol outlines our proposed methodology for conducting a systematic review. Our results will provide an update to the existing literature by searching additional databases. CONCLUSIONS: Findings from our systematic review will inform the planning of proper treatment methods that can effectively reduce the levels of ARB, ARGs, and residual antibiotics in effluent, thus lowering the risk of the environmental spread of AMR and its further transmission to humans and animals. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/33365

Ground State Vortex Lattice Structures in d-wave Superconductors

We show in a realistic $d_{x^{2}-y^{2}}$ symmetry gap model for a cuprate superconductor that the clean vortex lattice has discontinuous structural transitions (at and near T=0), as a function of the magnetic field $B$ along the c-axis. The transitions arise from the singular nonlocal and anisotropic susceptibility of the $d_{x^{2}-y^{2}}$ superconductor to the perturbation caused by supercurrents associated with vortices. The susceptibility, due to virtual Dirac quasiparticle-hole excitation, is calculated carefully, and leads to a ground state transition for the triangular lattice from an orientation along one of the crystal axis to one at 45$^o$ to them, i.e, along the gap zero direction. The field scale is seen to be 5 Tesla $\sim (\Delta_{0}/ta)^{2}\Phi_{0}$, where $\Delta_{0}$ is the gap maximum, $t$ is the nearest neighbour hopping, $a$ is the lattice constant, and $\Phi_{0}$ is the flux quantum. At much higher fields ($\sim 28T$) there is a discontinuous transition to a centred square structure. The source of the differences from existing calculations, and experimental observability are discussed, the latter especially in view of the very small (a few degrees $K$ per vortex) differences in the ground state energy.Comment: To be published in Phys. Rev.