Evidence of mature adipocyte proliferation regulated by proliferin

Despite much research, whether mature adipocytes proliferate remains controversial. Here, we examined 5-bromo-2′-deoxyuridine (BrdU)-labelling of mature adipocytes. Although BrdU incorporation into subcutaneous adipocytes was less than that in visceral adipocytes, pioglitazone (Pio) treatment increased BrdU incorporation in subcutaneous, but not visceral, adipocytes in rats. Fully differentiated 3T3-L1 adipocytes exhibited an increase in cell number and BrdU incorporation with time, with this increase enhanced by Pio treatment. We therefore screened for genes that encode growth factors regulated by Pio, and selected proliferin (PLF). Both gene silencing of PLF by small interfering RNA and treatment with anti-PLF antibody suppressed proliferation in 3T3-L1 adipocytes. In adipocytes isolated from Pio-treated rats, the tissue-specific pattern of PLF expression was similar to that of BrdU incorporation. Administration of an anti-PLF antibody to mice reduced BrdU incorporation into adipocytes. Mature adipocytes thus have the ability to replicate, and this proliferation is positively regulated by PLF

Blob/Hole Generation in the Divertor Leg of the Large Helical Device

We have analyzed ion saturation current fluctuation measured by a fast scanning Langmuir probe (FSP) in edge region of the Large Helical Device (LHD). Positive and negative spikes of the ion saturation current were observed in the private region and on the divertor leg, respectively. It was found that the boundary position between these regions corresponds to the low-field side (LFS) edge of the divertor leg where the gradient of the ion saturation current profile was the maximum. Such a positional relationship resembles that near the separatrix in the LFS in tokamaks, where blobs and holes are generated. Statistical analysis indicates similar fluctuation characteristics among different magnetic devices

Constructing a digital museum with a large-scale archive for endangered languages

In this presentation we will propose a design for a digital museum for endangered languages. Just like a real museum, the digital museum proposed here consists of (1) a storage space, where items are archived, and (2) an exhibition space, where a selection of items from the storage are exhibited. Currently, in language documentation and conservation, the archives and the web pages are treated separately. Language archives are created mainly for the purpose of storing language data permanently for future reference. The web spaces for language conservation or exhibition are usually constructed without direct reference to the archived data. In our previous work presented at the first ICLDC, we proposed a basic design for a digital museum and demonstrated its prototype, featuring Nishihara village, where Ikema, a dialect of Miyako, one of the endangered languages of Ryukyuan, is spoken. It consisted of three layered digital spaces, the first layer is used for the exhibition, the second for the storage of past exhibits and the third for the raw data. The proposed digital museum has been implemented by an open source content management platform, providing a webpage easily updatable and extendable to other languages, making a step forward in the documentation and conservation of endangered languages. The museum was made public early this year (www.kikigengo.jp). As it is, however, the site has not been linked to the data archives, mostly due to technical reasons. With the recent development of cloud technologies and services, however, we are now able to construct a digital museum, in which the large-scale archive space is directly linked with an exhibition space. The archive is constructed in a large-scale cloud space from which files can be directly linked to the web exhibit space. We will use an open source video asset management platform on the private cloud service at our university, which manages audio-visual files and controls the security and privacy of the archives constructed on the cloud. The archive space can be compartmentalized into “publishers,” each of which can serve as a distinct password protected archive for a different language conservation project. The publishers can also be used for exchanging files with other members of the same project. The system enables us to construct a digital space for endangered languages linked to a large-scale archive at an individual level and at a manageable price, thereby providing us with a powerful tool for language documentation and conservation

Predicting sentinel lymph node metastasis in breast cancer with lymphoscintigraphy

金沢大学医薬保健研究域保健学系Objective: Lymphoscintigraphy is an effective method for detecting sentinel lymph nodes (SLNs). However, the rate and degree of SLN detection is not uniform. We quantified SLNs detected with lymphoscintigraphy, and investigated correlations with factors that may influence detection. We then attempted to predict SLN metastasis from lymph node counts, comparing the predictions to subsequent biopsy results. Methods: We assessed lymph node counts in 100 breast cancer patients in whom a single SLN was detected with a fixed lymphoscintigraphy procedure. We examined correlations between the counts and factors known to influence lymphoscintigraphic SLN detection (age, body mass index, tumor size, and presence or absence of metastasis), and determined reference values (lymph node counts of 10.0, 19.4 and 53.0) which were used to predict SLN metastasis in 100 subsequent patients. The predictions were then compared with the SLN biopsy findings. Results: SLN counts correlated strongly with the presence or absence of metastasis, with metastasis-positive lymph nodes showing significantly lower counts than negative nodes (p < 0.001). Prediction of SLN metastasis achieved a 100% positive predictive value at a reference value of 10.0, and a 100% negative predictive value at a reference value of 53.0. At a reference value of 19.4, the sensitivity, specificity, and diagnostic accuracy were 77.8, 73.2, and 74.0%, respectively. Conclusions: The SLN counts detected with lymphoscintigraphy were significantly lower in metastasis-positive lymph nodes than in metastasis-negative lymph nodes. This suggests that prediction of SLN metastasis in breast cancer is possible using lymphoscintigraphy. © 2010 The Japanese Society of Nuclear Medicine

Postnatal Expression of BRAFV600E Does Not Induce Thyroid Cancer in Mouse Models of Thyroid Papillary Carcinoma

The mutant BRAF (BRAFV600E) is the most common genetic alteration in papillary thyroid carcinomas (PTCs). The oncogenicity of this mutation has been shown by some genetically engineered mouse models. However, in these mice, BRAFV600E is expressed in all the thyroid cells from the fetal periods, and suppresses thyroid function, thereby leading to TSH elevation, which by itself promotes thyroid tumorigenesis. To overcome these problems, we exploited 2 different approaches, both of which allowed temporally and spatially restricted expression of BRAFV600E in the thyroid glands. First, we generated conditional transgenic mice harboring the loxP-neoR-loxP-BRAFV600Einternal ribosome entry site-green fluorescent protein sequence [Tg(LNL-BRAFV600E)]. The double transgenic mice (LNL-BRAFV600E;TPO-Cre) were derived from a high expressor line of Tg(LNLBRAFV600E) mice and TPO-Cre mice; the latter expresses Cre DNA recombinase under the control of thyroid-specific thyroid peroxidase (TPO) promoter and developed PTC-like lesions in early life under normal serum TSH levels due to mosaic recombination. In contrast, injection of adenovirus expressing Cre under the control of another thyroid-specific thyroglobulin (Tg) promoter (Ad-TgP-Cre) into the thyroids of LNL-BRAF V600E mice did not induce tumor formation despite detection ofBRAFV600EandpERKin a small fraction of thyroid cells. Second, postnatal expression ofBRAFV600E in a smallnumberof thyroid cellswasalso achieved by injecting the lentivirus expressing loxP-green fluorescent protein-loxP-BRAFV600E into the thyroids of TPO-Cre mice; however, no tumor development was again observed. These results suggest that BRAFV600E does not appear to induce PTC-like lesions when expressed in a fraction of thyroid cells postnatally under normal TSH concentrations

Search for gravitational-lensing signatures in the full third observing run of the LIGO-Virgo network

Gravitational lensing by massive objects along the line of sight to the source causes distortions of gravitational wave-signals; such distortions may reveal information about fundamental physics, cosmology and astrophysics. In this work, we have extended the search for lensing signatures to all binary black hole events from the third observing run of the LIGO--Virgo network. We search for repeated signals from strong lensing by 1) performing targeted searches for subthreshold signals, 2) calculating the degree of overlap amongst the intrinsic parameters and sky location of pairs of signals, 3) comparing the similarities of the spectrograms amongst pairs of signals, and 4) performing dual-signal Bayesian analysis that takes into account selection effects and astrophysical knowledge. We also search for distortions to the gravitational waveform caused by 1) frequency-independent phase shifts in strongly lensed images, and 2) frequency-dependent modulation of the amplitude and phase due to point masses. None of these searches yields significant evidence for lensing. Finally, we use the non-detection of gravitational-wave lensing to constrain the lensing rate based on the latest merger-rate estimates and the fraction of dark matter composed of compact objects