Etorphine-azaperone immobilisation for translocation of free-ranging masai giraffes (Giraffa camelopardalis tippelskirchi): A pilot study

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Etorphine-azaperone immobilisation was evaluated for translocation of Masai giraffes. Nine giraffes were darted with 0.012 ± 0.001 mg/kg etorphine and 0.07 ± 0.01 mg/kg azaperone. Once ataxic, giraffes were roped for recumbency and restrained manually. Naltrexone (3 mg/mg etorphine) was immediately given intravenously to reverse etorphine-related side effects. Protocol evaluation included physiological monitoring, blood-gas analyses, anaesthetic times, and quality scores (1 = excellent, 4 = poor). Sedation onset and recumbency were achieved in 2.6 ± 0.8 and 5.6 ± 1.4 min. Cardio-respiratory function (HR = 70 ± 16, RR = 32 ± 8, MAP = 132 ± 16) and temperature (37.8 ± 0.5) were stable. Arterial gas analysis showed hypoxaemia in some individuals (PaO2 = 67 ± 8 mmHg) and metabolic acidosis (pH = 7.23 ± 0.05, PaCO2 = 34 ± 4 mmHg, HCO3− = 12.9 ± 1.2 mmol/l). Minor startle response occurred, while higher induction-induced excitement correlated to longer inductions, worse restraint, and decreased HCO3−. After 19 ± 3.5 min of restraint, giraffes were allowed to stand and were loaded onto a chariot. Immobilisations were good and scored 2 (1–3). Inductions and recoveries were smooth and scored 1 (1–2). Translocations were uneventful and no complications occurred in 14-days boma follow-up

Surgical treatment of an umbilical hernia in a free-ranging sub-adult African elephant in Samburu National Reserve, Kenya

Domnic Mijele, Michael Njoroge, Titus Kaitho Veterinary Services Department, Species Conservation and Management Division, Kenya Wildlife Service, Nairobi, Kenya Abstract: A 10-year-old male African elephant (Loxodonta africana) at Samburu National Reserve in Northern Kenya, weighing approximately 1,600 kg, presented with an umbilical hernia in October 2013. Umbilical herniorrhaphy was carried out under field conditions. Anesthesia was induced and maintained using etorphine hydrochloride for 3 hours during the surgery. This case report details both the surgical and anesthetic procedure carried out to correct the hernia, and the eventual successful recovery of the elephant from anesthesia. However, the elephant died weeks after the surgery and a postmortem could not reveal the cause of death because predators had scavenged the carcass. The challenges of the surgical procedure and outcome including possible causes of death are highlighted in this report. Keywords: African elephant, general anesthesia, etorphine hydrochloride, local anesthesia Lignocaine + adrenaline, umbilical herniorrhaph

Dystocia in a Rothschild Giraffe at the African Fund for Endangered Wildlife, Nairobi, Kenya

A 15-year old female Rothschild Giraffe (Giraffa camelopardalis rothschildi) weighing approximately 800kg, at the African Fund for Endangered Wildlife (AFEW), Giraffe Center, Langata, Nairobi, Kenya was presented with dystocia in June 2010. This giraffe named Laura, had a protracted labor and was regularly monitored by sanctuary education staff. Dystocia was relieved on the 3rd day at this wildlife sanctuary. The giraffe was chemically immobilized by using 7mg of Etorphine Hcl (0.98%) (M99®) (Norvatis South Africa (Pty) Limited) and 50mg of Azaperone(10%) (Kyron Laboratories (Pty) Limited, South Africa) in a Dan-Inject dart (Dan-inject APS, Sellerup Skowej, Denmark). On obstetrical examination of the giraffe, a fetal malposition type of dystocia had occurred. The fetus was positioned at posterior presentation extended posture with tail butting on the maternal pelvis, which is abnormal in giraffes. The fetus was manually extracted by using both alternate and simultaneous limb traction. The dam survived the procedure and later was reported to be in a good reproductive condition but the male fetus was a stillbirth. The fetus had died due to stress of prolonged labour. Relief of dystocia in giraffes is a difficult obstetrical procedure because obstetrical examination and relief requires chemical immobilization plus physical restrain with ropes by trained staff. Anesthesia or immobilization of giraffes remains a challenge because of the giraffe's unique anatomy and physiology. Giraffes are large animals which limits physical control and manipulation at critical times during induction and recovery of anesthesia. Giraffe's long neck if not pinned to the ground will act as a lever causing fatal injuries to self and support staff. Giraffes develop elevated systolic blood pressure; have a small respiratory tidal volume with a large dead space and relatively small cardiac output during anesthesia, which compromises safe levels of anesthesia. [Vet. World 2011; 4(12.000): 565-568

Economic analysis of cross-breeding programmes in sub-Saharan Africa: A conceptual framework and Kenyan case study

A conceptual framework for evaluating cross-breeding programmes in sub-Saharan Africa is developed based on a Kenyan case study. It depicts livestock production as a system where farm animals, plants, land and water are interlinked in particular ways and are also interlinked with the environment. Depending on the level of intensification and `modernisation', two livestock systems are defined. The first is the traditional livestock production system in which farm animals, plants, land and water are interlinked in a sustainable way with each component complementing the other. The second is a'modern' production system based on crossbreds with varying levels of exotic dairy cattle breeds or pure exotic dairy cattle breeds. Successful cross-breeding not only weakens the interlinkages in the traditional system, but also creates new linkages with external inputs. It is argued that the weakened existing and the newly formed linkages ought to be taken into account when cross-breeding programmes are being evaluated. To accurately evaluate cross-breeding programmes it is, therefore, important to delineate all the outputs and inputs of such a system. In this Report, the outputs and inputs of cattle cross-breeding programmes are defined. Outputs include the marketable products of milk, meat, manure, animal draft power, hides and skins. Inputs include the conventional ones such as research infrastructure, equipment and personnel, extension services, disease control services, exotic germplasm, indigenous germplasm, feeds and marketing infrastructure. Cross-breeding also entails loss of the non-marketed outputs and values of indigenous livestock, such as cultural value, wealth function, existence value, option value and recreation value. An attempt was made to demonstrate the applicability of the developed conceptual framework using the case of cross-breeding Zebu cattle with exotic breeds for dairy improvement in Kenya. Due to time and resource constraints, already existing models that were not specifically developed for the task envisaged in the conceptual framework were applied in the analysis. Some important variables could not be included in the analysis due to data and model limitations. Welfare effects of cross-breeding programmes were estimated using the Kenya Agricultural Sector Model (ASM). The impact of cross-breeding at the farm level was analysed using the Farm Level Income and Policy Simulation Model (FLIPSIM). The two models were developed by the Impact Assessment Group (2000) of Texas A&M University, USA, and applied to evaluate the impact of improved dairy technologies in Kenya in collaboration with the Kenya Agricultural Research Institute (KARI) and the International Livestock Research Institute (ILRI). In this study, these models were used specifically to provide estimates of the economic benefits of crossbreeding indigenous Zebu cattle with exotic dairy breeds. Despite the data and model limitations, the analyses provided useful insights into the benefits and costs of breeding programmes in Kenya

Participatory Approaches in the Control of Camel Trypanosomosis in Lapur Division of Turkana District, Kenya

This study was conducted to evaluate the control of trypanosomosis in camels in Turkana district of Kenya using participatory approaches. Lapur division of the district was conveniently selected as the study area considering logistics and security concerns. Four main animal camps (adakars) formed the study units. Key informants from each adakar were selected for participatory research processes. Participatory mapping, semi-structured interviews, pair-wise comparisons and matrix scoring were the participatory methods employed. Five camel diseases in order of their importance, were identified, namely, camel trypanosomosis, tick infestation, non-specific diarrhea, mange and hemorrhagic septicemia. Twelve groups of the lay key informants agreed well on the presenting signs of these diseases. Although trypanocides were considered by the informants to be reasonably available, the most preferred method for the control of camel trypanosomosis was the use of indigenous remedies. These indigenous remedies included the oral administration to sick camels with variety of herbs mixed with soups from goat, wildcat, bird or donkey meat. The results from this study revealed that camel trypanosomosis is an important disease in Turkana district. The prices of the available modern trypanocides in the management of camel trypanosomosis appeared to hamper the effective control of the disease. However, the efficacy of the widely used indigenous remedies remains undetermined The Kenya Veterinarian Vol. 30 (1) 2006: pp. 1-1

Genomic epidemiology of Escherichia coli: antimicrobial resistance through a One Health lens in sympatric humans, livestock and peri-domestic wildlife in Nairobi, Kenya

Background Livestock systems have been proposed as a reservoir for antimicrobial-resistant (AMR) bacteria and AMR genetic determinants that may infect or colonise humans, yet quantitative evidence regarding their epidemiological role remains lacking. Here, we used a combination of genomics, epidemiology and ecology to investigate patterns of AMR gene carriage in Escherichia coli, regarded as a sentinel organism. Methods We conducted a structured epidemiological survey of 99 households across Nairobi, Kenya, and whole genome sequenced E. coli isolates from 311 human, 606 livestock and 399 wildlife faecal samples. We used statistical models to investigate the prevalence of AMR carriage and characterise AMR gene diversity and structure of AMR genes in different host populations across the city. We also investigated household-level risk factors for the exchange of AMR genes between sympatric humans and livestock. Results We detected 56 unique acquired genes along with 13 point mutations present in variable proportions in human and animal isolates, known to confer resistance to nine antibiotic classes. We find that AMR gene community composition is not associated with host species, but AMR genes were frequently co-located, potentially enabling the acquisition and dispersal of multi-drug resistance in a single step. We find that whilst keeping livestock had no influence on human AMR gene carriage, the potential for AMR transmission across human-livestock interfaces is greatest when manure is poorly disposed of and in larger households. Conclusions Findings of widespread carriage of AMR bacteria in human and animal populations, including in long-distance wildlife species, in community settings highlight the value of evidence-based surveillance to address antimicrobial resistance on a global scale. Our genomic analysis provided an in-depth understanding of AMR determinants at the interfaces of One Health sectors that will inform AMR prevention and control