Integrating magnetic capabilities to intracellular chips for cell trapping

Current microtechnologies have shown plenty of room inside a living cell for silicon chips. Microchips as barcodes, biochemical sensors, mechanical sensors and even electrical devices have been internalized into living cells without interfering their cell viability. However, these technologies lack from the ability to trap and preconcentrate cells in a specific region, which are prerequisites for cell separation, purification and posterior studies with enhanced sensitivity. Magnetic manipulation of microobjects, which allows a non-contacting method, has become an attractive and promising technique at small scales. Here, we show intracellular Ni-based chips with magnetic capabilities to allow cell enrichment. As a proof of concept of the potential to integrate multiple functionalities on a single device of this technique, we combine coding and magnetic manipulation capabilities in a single device. Devices were found to be internalized by HeLa cells without interfering in their viability. We demonstrated the tagging of a subpopulation of cells and their subsequent magnetic trapping with internalized barcodes subjected to a force up to 2.57 pN (for magnet-cells distance of 4.9 mm). The work opens the venue for future intracellular chips that integrate multiple functionalities with the magnetic manipulation of cells

Neural network identification of people hidden from view with a single-pixel, single-photon detector

Light scattered from multiple surfaces can be used to retrieve information of hidden environments. However, full three-dimensional retrieval of an object hidden from view by a wall has only been achieved with scanning systems and requires intensive computational processing of the retrieved data. Here we use a non-scanning, single-photon single-pixel detector in combination with a deep convolutional artificial neural network: this allows us to locate the position and to also simultaneously provide the actual identity of a hidden person, chosen from a database of people (N = 3). Artificial neural networks applied to specific computational imaging problems can therefore enable novel imaging capabilities with hugely simplified hardware and processing times

Multimodality Imaging of Abnormal Vascular Perfusion and Morphology in Preclinical 9L Gliosarcoma Model

This study demonstrates that a dynamic susceptibility contrast-magnetic resonance imaging (DSC-MRI) perfusion parameter may indicate vascular abnormality in a brain tumor model and reflects an effect of dexamethasone treatment. In addition, X-ray computed tomography (CT) measurements of vascular tortuosity and tissue markers of vascular morphology were performed to investigate the underpinnings of tumor response to dexamethasone.One cohort of Fisher 344 rats (N = 13), inoculated intracerebrally with 9L gliosarcoma cells, was treated with dexamethasone (i.p. 3 mg/kg/day) for five consecutive days, and another cohort (N = 11) was treated with equal volume of saline. Longitudinal DSC-MRI studies were performed at the first (baseline), third and fifth day of treatments. Relative cerebral blood volume (rCBV) was significantly reduced on the third day of dexamethasone treatment (0.65 ± .13) as compared to the fifth day during treatment (1.26 ±.19, p < 0.05). In saline treated rats, relative CBV gradually increased during treatment (0.89 ±.13, 1.00 ± .21, 1.13 ± .23) with no significant difference on the third day of treatment (p>0.05). In separate serial studies, microfocal X-ray CT of ex vivo brain specimens (N = 9) and immunohistochemistry for endothelial cell marker anti-CD31 (N = 8) were performed. Vascular morphology of ex vivo rat brains from micro-CT analysis showed hypervascular characteristics in tumors, and both vessel density (41.32 ± 2.34 branches/mm(3), p<0.001) and vessel tortuosity (p<0.05) were significantly reduced in tumors of rats treated with dexamethasone compared to saline (74.29 ± 3.51 branches/mm(3)). The vascular architecture of rat brain tissue was examined with anti-CD31 antibody, and dexamethasone treated tumor regions showed reduced vessel area (16.45 ± 1.36 µm(2)) as compared to saline treated tumor regions (30.83 ± 4.31 µm(2), p<0.001) and non-tumor regions (22.80 ± 1.11 µm(2), p<0.01).Increased vascular density and tortuosity are culprit to abnormal perfusion, which is transiently reduced during dexamethasone treatment

You don't see the world through the same eyes any more: The impact of sexual offence work on police staff

This paper examines the experiences of Police staff in England who work with sexual offence material (SOM). Eleven officers completed a questionnaire then took part in semi-structured interviews. The data were analysed in two stages: Interpretative Phenomenological Analysis was used to illuminate the ‘lived experience’ of participants, and establish a theme structure. Clinical models of workplace trauma were then employed to explore the theme ‘Impact of working with sexual offending’. Impact includes cognitive intrusions and increased suspiciousness. The authors identify where officers’ accounts intersect with nascent symptoms of both Vicarious Traumatisation (McCann and Pearlman, 1990) and Post-Traumatic Stress Disorder (PTSD)

Abnormalities of calcium metabolism and myocardial contractility depression in the failing heart

Heart failure (HF) is characterized by molecular and cellular defects which jointly contribute to decreased cardiac pump function. During the development of the initial cardiac damage which leads to HF, adaptive responses activate physiological countermeasures to overcome depressed cardiac function and to maintain blood supply to vital organs in demand of nutrients. However, during the chronic course of most HF syndromes, these compensatory mechanisms are sustained beyond months and contribute to progressive maladaptive remodeling of the heart which is associated with a worse outcome. Of pathophysiological significance are mechanisms which directly control cardiac contractile function including ion- and receptor-mediated intracellular signaling pathways. Importantly, signaling cascades of stress adaptation such as intracellular calcium (Ca2+) and 3′-5′-cyclic adenosine monophosphate (cAMP) become dysregulated in HF directly contributing to adverse cardiac remodeling and depression of systolic and diastolic function. Here, we provide an update about Ca2+ and cAMP dependent signaling changes in HF, how these changes affect cardiac function, and novel therapeutic strategies which directly address the signaling defects

FEDSM2003-45490 PIV INVESTIGATIONS OF THE FLOW FIELD IN THE VOLUTE OF A ROTARY BLOOD PUMP

ABSTRACT A full-size acrylic model of a rotary blood pump was developed in order to utilize Particle Image Velocimetry (PIV) to make measurements of the fluid velocities and turbulent stresses throughout the device. The development of an understanding of the hemodynamics within the blood pump is critical to the development and validation of computational models. A blood analog solution, consisting of sodium iodide solution and glycerin, was developed to match physiological kinematic viscosity. The refractive indecies of the fluid, the pump casing and the impeller were matched to facilitate the use of PIV to make velocity measurements. Velocity measurements made in the volute exit/diffuser region are presented for pumps speeds of 3000-3850 rpm. At each speed data were obtained at a physiological pressure of 90 mmHg and at a maximum flow condition. Four hundred data pairs were used for each resultant mean velocity vector value, representing greater than an order of magnitude more data pairs than reported previously in the literature on similar devices and resulting in velocity uncertainty levels of approximately ±2.9%

A customised portable LogMAR chart with adjustable chart illumination for use as a mass screening device in the rural population

<b>Aim:</b> To develop a customised, portable, cost-effective (logarithmic minimal angle resolution) LogMAR chart with adjustable illumination for use as a mass vision-screening device in the rural population. <b> Materials and Methods</b> : Visual acuity of 100 individuals was evaluated with a customised chart and compared with the standard Early Treatment Diabetic Retinopathy Study (ETDRS) chart and Snellen&#x2032;s Chart. Bland and Altman analytical techniques were used for analysis. <b> Results: </b> Test-retest variability of the customised chart was just a one-line difference (95&#x0025; CI for agreement), and so were the results with the standard ETDRS charts; a variability of 3-line was noted with Snellen&#x2032;s chart. Two-line differences were observed when comparison was made with Standard ETDRS chart and 2 to 3-line differences with Snellen&#x2032;s chart. <b> Conclusion: </b> The customised portable LogMAR chart with adjustable illumination shows less test-retest variability and better agreement with standard ETDRS chart; therefore, it can be used as a mass vision-screening device in rural settings

A customised portable LogMAR chart with adjustable chart illumination for use as a mass screening device in the rural population

Aim: To develop a customised, portable, cost-effective (logarithmic minimal angle resolution) LogMAR chart with adjustable illumination for use as a mass vision-screening device in the rural population. Materials and Methods : Visual acuity of 100 individuals was evaluated with a customised chart and compared with the standard Early Treatment Diabetic Retinopathy Study (ETDRS) chart and Snellen\u2032s Chart. Bland and Altman analytical techniques were used for analysis. Results: Test-retest variability of the customised chart was just a one-line difference (95% CI for agreement), and so were the results with the standard ETDRS charts; a variability of 3-line was noted with Snellen\u2032s chart. Two-line differences were observed when comparison was made with Standard ETDRS chart and 2 to 3-line differences with Snellen\u2032s chart. Conclusion: The customised portable LogMAR chart with adjustable illumination shows less test-retest variability and better agreement with standard ETDRS chart; therefore, it can be used as a mass vision-screening device in rural settings

Neural RF SLAM for unsupervised positioning and mapping with channel state information

We present a neural network architecture for jointly learning user locations and environment mapping up to isometry, in an unsupervised way, from channel state information (CSI) values with no location information. The model is based on an encoder-decoder architecture. The encoder network maps CSI values to the user location. The decoder network models the physics of propagation by parametrizing the environment using virtual anchors. It aims at reconstructing, from the encoder output and virtual anchor location, the set of time of flights (ToFs) that are extracted from CSI using super-resolution methods. The neural network task is set prediction and is accordingly trained end-to-end. The proposed model learns an interpretable latent, i.e., user location, by just enforcing a physics-based decoder. It is shown that the proposed model achieves sub-meter accuracy on synthetic ray tracing based datasets with single anchor SISO setup while recovering the environment map up to 4cm median error in a 2D environment and 15cm in a 3D environmentComment: Accepted at IEEE International Conference on Communications 2022. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other wor