ANTIOXIDANT AND THE IMMUNOMODULATORY ACTIVITIES EXHIBITED BY THREE PLANTS FROM LAMIACEAE FAMILY

Objective: The aim of this work was screening of the antioxidant activity and immunomodulatory effects of the crude extracts from the aerial parts of three Algerian endemic species from Lamiaceae.Methods: DPPH radical scavenging assay was used to find the antioxidant activity of Stachys circinata, Salvia verbenaca and Thymus guyonii and the immunostimulant potential of these plants extracts on the phagocytic activity which was measured by the carbon clearance rate test.Results: Our results obtained in this study shown that the IC50 of Thymus guyonii, Salvia verbenaca and Stachys circinata were 15,90;47,50 and 54,92 respectively. The phagocytic index was increased significantly in animals injected with Stachys circinata and Salvia verbenaca at doses of 200 mg/kg P≤0,05 but not significantly with Thymus guyonii when compared to the control group. The clearance rate of carbon was faster but not significantly P≥ 0,05 and the corrected phagocytic index α was increased at the group GIII with Salvia verbenaca but not significantly when compared with other groups P≥ 0,05.Conclusion: The Stachys circinata and Salvia verbenaca extracts proved an immune-stimulatory effect on the reticuloendothelial system and Thymus guyonii extract revealed anti-oxidant activity.Â

Antioxidant abietane diterpenoids from Salvia barrelieri

The root extract of endemic Algerian Salvia species Salvia barrelieri Ettling and its diterpenoids were investigated for potential antioxidant activity. From its acetone extract, a new natural abietane diterpenoid 7-oxoroyleanone-12-methyl ether (1) and six known diterpenoids 7 alpha-acetoxyroyleanone-12-methyl ether (2), royleanone (3), horminone (4), 7-acetylhorminone (5), cryptojaponol (6) and inuroyleanol (7) were isolated, and their structures were elucidated by spectroscopic means. Among the diterpenoids, the new diterpenoid 7-oxoroyleanone-12-methyl ether (1) showed highest superoxide anion scavenging activity while inuroyleanol (7) showed both the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and inhibition of lipid peroxidation in beta-carotene-linoleic acid system. These findings indicate that S. barrelieri extract as well as isolated abietane diterpenes, particularly inuroyleanol are promising antioxidants which can be used as food additives. (c) 2006 Elsevier Ltd. All rights reserved

Antioxidant Diterpenoids from the Roots of Salvia barrelieri

Introduction - The phytochemical and biological studies carried out on Salvia species showed that their extracts and constituents have various biological activities

Triterpenoid saponins from Scabiosa stellata collected in North-eastern Algeria

International audienceEight undescribed triterpenoid saponins, scabiostellatosides A-H, together with three known compounds were isolated from the whole plant of Scabiosa stellata L. Their structures were established by spectroscopic analyses (1D, 2D-NMR and HRESIMS) and chemical methods. Scabiostellatosides A-H were evaluated for their cytotoxicity against human fibrosarcoma cell line (HT1080). Scabiostellatoside F, a heptasaccharide derivative of oleanolic acid, exhibited moderate cytotoxicity against HT1080 cell line with IC50 value of 12.0 μM

Abietane diterpenes from the cones of Abies numidica de Lannoy ex Carrière (Pinaceae) and in vitro evaluation of their antimicrobial properties

International audienceEight known abietane diterpenes (1-8) were isolated for the first time from Abies numidica cones (Pinaceae). The structures of all compounds were established by means of 1D and 2D NMR, and UV spectral analyses. The hydromethanolic extract of A. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. The best antimicrobial activity was found against gram-positive bacteria (MIC ≤ 0.3 mg/mL) Bacillus subtilis, Enterococcus faecalis ATCC 1034, Staphylococcus aureus 8325.4, S. aureus CIP 53.154, Micrococcus luteus and Listeria innocua and against Candida yeasts. The determination of MIC's of isolated products showed a high activity of compounds 4 and 6 against S. aureus, L. innocua (MIC = 62.5 μg/mL) and E. faecalis (MIC = 125 μg/mL)

A new sulfonylated flavonoid and other bioactive compounds isolated from the aerial parts of Cotula anthemoides L.

International audienceA new sulfonyl flavonol glucoside, 5,7,4′,5′-tetrahydoxyflavonol 2′-[propanoic acid-(2″′-acetoxy-1″′-sulfonyl)]-5′-O-β-d-glucopyranoside (1) was isolated from the aerial parts of Cotula anthemoides L. in addition to 15 known compounds (2–16). The structure elucidation of these compounds was based on analyses of spectroscopic data including 1D-, 2D-NMR and HR-ESI-MS techniques and by comparing their NMR data with those reported in the literature. These compounds were evaluated for their DPPH radical scavenging and tyrosinase inhibitory activity. Compound 6 showed a high DPPH radical scavenging with EC50 value of 9.1 ± 0.4 μM. Compound 11, 9 and 1 exhibited a mild tyrosinase inhibitory activity with IC50 values of 85 ± 0.8, 95 ± 1.5 and 100 ± 0.5 μM, respectively

6"'- O -acetylisospinosin, a new C -glycosylflavone and known compounds from the aerial parts of Cladanthus mixtus

International audienceA new C-glycosylflavone, identified as 6"'-O-acetylisospinosin (1), was isolated from the aerial parts of Cladanthus mixtus together with 30 known compounds. The structures of these compounds were established by interpretation of their spectral data, mainly UV, 1D and 2D NMR spectroscopic methods including (1H and 13C NMR, COSY, ROESY, HSQC and HMBC experiments), ESI-MS, and by comparison with the literature data

GC/MS analysis and analgesic effect of the essential oil of Matricaria pubescens from Algeria.

International audienceThe essential oil of Matricaria pubescens (Asteraceae) collected at Ghardaia (Algerian Septentrional Sahara) was studied by GC and GC-MS. Isochrysanthemic acid ethyl ester (26.5%), spathulenol (19.4%), alpha-cadinol (12.9%) and geranylisovalerate (8.2%), were identified as the major components of the essential oil, which was investigated for its analgesic effect