GTPase-activating Protein-binding Protein 1 Plays an Antiviral Role in Porcine Epidemic Diarrhea Virus (PEDV) Replication

Porcine Epidemic Diarrhea Virus (PEDV) is a non-segmented virus which uses one-stranded, positive-sense RNA as genetic material. PEDV falls under Coronaviridae family and extensive diarrhea and dehydration in nursing piglets are the major clinical signs. This emerging disease leads to huge economic loss in the pig farming. Very less studies are done towards the role of innate immunity in PEDV infection. The formation of Stress granules (SGs) are seen when cells are introduced to different stressful conditions, which also includes viral infections. SGs are formed when one of the four kinases: doublestranded RNA (dsRNA)-dependent protein kinase (PKR), PKR-like endoplasmic reticulum kinase (PERK), general control nonderepressible 2 (GCN2) kinase and hemeregulated inhibitor kinase (HRI), causes phosphorylation of eukaryotic translation initiation factor (eIF2α). The alternative pathway of SG formation is eIF4A RNA helicase inhibition. Phosphorylation of eIF2α by PKR activation is the most common pathway of SGs formation. SGs are considered as an indication of an antiviral innate response of the host that limits translation of the viral genes. Ras-GTPase-activating protein (SH3 domain) binding protein 1 (G3BP1) is one of the important stress granule-resident protein that nucleates stress granule assembly. The objective of the study was to investigate the role of G3BP1 in PEDV replication. We found that depletion of G3BP1 inhibits SGs formation and overexpression of G3BP1 nucleates SGs assembly. We observed that knockdown of G3BP1 by silencing RNA significantly increased PEDV replication. Similarly, overexpression of exogenous G3BP1 lowered virus replication by approximately 100-fold compared to vector control. We also observed that PEDV-infected cells are resistant to SGs formation upon sodium arsenite treatment. An enhancement in the levels of mRNAs of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor - α (TNF-α) was also observed in PEDV-infected G3BP1 knockdown cells compared to PEDV-infected control cells. Taken together, our results demonstrate that PEDV induces transient SGs formation and G3BP1 plays an antiviral role in virus replication

The Use of MATLAB Programming to Compare Experimental vs Modeled PEMFCs using the Nernst and Butler-Volmer’s Equation-Based Mathematical Models

For the analysis of  Proton Exchange Membrane Fuel Cell (PEMFC’s) efficiency, the Nernst equation and Butler-Volmer's concepts were used. The mathematical models using both equations were developed in MATLAB and compiled. The results generated by the output current based on the input parameters of the experimental data were compared with the experimental results for the two modelled PEMFCs. The parameters temperature, pressure, hydrogen concentration, and oxygen concentration at different values of external resistance were used to determine the change in output current in both models built in MATLAB. This sensitivity analysis generated negative output current values and highly dissimilar values with the experimental results for the same input parameters for both models due to the less use of input parameters in the model. The results showed that the PEMFC's performance is affected by most parameters, and many influencing parameters must be used to develop a perfect mathematical model of the PEMFC.       &nbsp

Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India

Background. Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies. NAT combines the advantages of direct and highly sequence-specific detection of viral genomes. We analysed the performance of newer Procleix Ultrio Elite (PUE) and Procleix Ultrio assay (PUA) for the screening of the viral markers in our donor population. Material and Methods. 10,015 donor samples were screened by routine immunoassays and both versions of NAT. NAT yields detected were subjected to viral load estimation and to other serological markers. Results. A total of 21 NAT yields were detected; three were positive by both NAT systems, whereas 18 samples were reactive by PUE only. NAT yields include 18 HBV and 3 HCV yields, of which 17 HBV yields were occult infections and 1 was window period (WP) infection. All 3 HCV yields were WP infections. No HIV-1/HIV-2 yield was found. Conclusion. Efficient target capture chemistry in the new TMA assay version significantly improved sensitivity. NAT is superior to serological immunoassays for screening of the viral markers; and the efficient target capture system in the newer TMA assay, namely, the PUE system, has significantly improved sensitivity over the earlier versions

Shisham (Dalbergia sissoo) decline by dieback disease, root pathogens and their management: a review

Shisham or sissoo (Dalbergia sissoo) is an important multipurpose tree with great economic importance, but this tree has been infected by various root pathogens. This review article shows the works conducted on root pathogens and die back disease of Shisham and their management. Around seventy-one endophytic fungus has been found in sissoo trees in Nepal. Several fungi, including, Fusarium solani, F. oxysporum, Ganoderma lucidum, Phellinus gilvus, Polypours gilvus, Rhizoctonia solani, Polyporus spongiosum, etc. cause sissoo diseases. Ganoderma Lucidum and F. Solani are two main pathogenic agents in Shisham, all of which causes root rot and vascular wilt diseases, and are the causes for the large-scale death of this tree species. Root rot ganoderma is wide spread in both natural and plant-based forests. Older trees in Shisham are usually attacked by these pathogens and cause large-scale death. However, when sissoo is grown as a re-forested pure plant without the removal of the stumps or root of the initial plant, a serious problem of root rot can develop. Field sanitation and proper management of field are necessary to control the fungal diseases of Shisham. Another deleterious disease of  Shisham is dieback disease, where sissoo plantations have been confirmed to this disease when the infected trees begin to get dry from the top. There is no suitable solution for control of dieback of Shisham. There is a need of developing resistant varieties and to improve the quality of seed. This review may be useful tool for Forest Pathologists and other persons who are working in forestry and natural conservation sectors. &nbsp

Role of nutrients in wheat: a review

Wheat (Triticum aestivum L.) is an important cereal crop that provides ample nutritious calories for humans and animals. The nutrient plays a vital role in the production of wheat. In this review, previous works were evaluated to investigate the role of nutrients, nutrient deficiency and toxicity in wheat. Both macro and micronutrients are necessary for wheat plants. Every nutrient has its own character and is involved in different metabolic processes of plant life. Nutrient deficiency and toxicity conditions inhibit normal plant growth and exhibit characteristic symptoms. For optimal growth, development, and production, plants need all the necessary nutrients in balance. A balanced application of the primary nutrients (N, P, K), secondary nutrient (S) and some other micronutrients (Zn, B) are needed to enhance wheat production. The soil tests and the demand for crop nutrients should be assessed to identify the quantity of fertilizer recommended for the crop. This study would be a valuable means to wheat growers and researchers for sustainable and higher wheat production

Profile of Patients under Mechanical Ventilation in ICU during COVID-19 Pandemic: a Hospital Based Study

Background: COVID-19 has become unprecedented challenge to ICU management. The measures for containment of the disease and mitigation can be possible only with a proper and organized planning. This study was conducted to evaluate the profile of mechanically ventilated patient in ICU of a Hospital in remote high altitude region during this pandemic. Methods: A cross-sectional study was done in patients who were admitted and managed with intubation and mechanical ventilation in ICU of Karnali Academy of Health Sciences (KAHS). Data were collected and filled in per-forma. Data were filled in Microsoft Excel and analysis done. Result: Most of the cases were adult males with 50% average intubation days less than five. Out of the total 42 cases 67% were intubated for inadequate airway protection and 33% `for respiratory failure. PCR test for COVID-19 was done for 50% of cases and one patient got positive result. Mortality was around 50% among intubated patients in ICU. Conclusion: ICU services at Karnali Academy of Health Sciences have been provided for both COVID and non-COVID patients during the pandemic. Referral hospital ICUs in remote part are important in management of serious cases of those areas during any pandemic so they should be well prepared. Keywords: COVID-19, intubation, mechanical ventilation, pandemic

Post-harvest practices and loss assessment in tomato (Solanum lycopersicum L.) in Kathmandu, Nepal

Postharvest loss is one of the major problems in tomato production. This study was conducted to assess the post-harvest practices and losses of tomato at the farmers’ fields in three districts namely Kathmandu (Kavresthali), Lalitpur (Lubhoo, and Lamatar), and Bhaktapur (Tathali and Kaushaltar). By using a simple random sampling method, the sample size of 60 respondents was chosen. A pre-tested questionnaire, group conversation, and key-informant survey were applied in this study. The results revealed that 10.3% of the respondents produced the tomato on a small scale whereas 58.8% of respondents produced on large scale. 10% of total losses were found from harvesting to marketing. In the field, during harvesting and packaging, there was a 2% loss. The tomato loss was negligible during grading and washing. 4% loss was found during the transportation of tomato and 2% of the loss during the storage of tomato. To the producer level, the insufficient knowledge about the storage, packaging, transportation, and random harvesting led to the maximum loss of tomato. In the study area, the producers played important role in reducing the postharvest loss of tomato, therefore, public awareness should be increased through mass media about the proper harvesting and storing of tomato. &nbsp

Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions

Host innate immune response follows severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and it is the driver of the acute respiratory distress syndrome (ARDS) amongst other inflammatory end-organ morbidities. Such life-threatening coronavirus disease 2019 (COVID-19) is heralded by virus-induced activation of mononuclear phagocytes (MPs; monocytes, macrophages, and dendritic cells). MPs play substantial roles in aberrant immune secretory activities affecting profound systemic inflammation and end-organ malfunctions. All follow the presence of persistent viral components and virions without evidence of viral replication. To elucidate SARS-CoV- 2-MP interactions we investigated transcriptomic and proteomic profiles of human monocyte-derived macrophages. While expression of the SARS-CoV-2 receptor, the angiotensin-converting enzyme 2, paralleled monocyte-macrophage differentiation, it failed to affect productive viral infection. In contrast, simple macrophage viral exposure led to robust pro-inflammatory cytokine and chemokine expression but attenuated type I interferon (IFN) activity. Both paralleled dysregulation of innate immune signaling pathways, specifically those linked to IFN. We conclude that the SARS-CoV-2-infected host mounts a robust innate immune response characterized by a pro-inflammatory storm heralding end-organ tissue damage

Severe COVID19 Patients and a 3D Airway Tissue Model of SARS-CoV-2 Infection Express High Levels of Airway Mucins and Associated Immunomodulatory Long Noncoding RNAs

Background: Respiratory epithelial cells (RECs) lining the upper airways are primary entry-point for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent for coronavirus disease 2019 (COVID19). Managing the overwhelming inflammatory response and mucus hypersecretion are among the major challenges faced in achieving effective treatment. The present study analyzes the acute inflammatory and mucous responses following SARS-CoV-2 infection. Methods: Nasal swabs from COVID19 patients with mild and severe pathologies were analyzed for the expression of viral RNA (vRNA) for nucleocapsid (N) and spike (S) proteins, viral entry regulating host factors (ACE-2 and TMPRSS-2), epithelial inflammatory factors (IL-6, ICAM-1 and CXCL-8), respiratory mucins (MUC1, 2, 4, 5AC, 5B, 7, 16 and 19), mucin regulatory transcription factors (SPDEF and FOXA3) and select long noncoding RNAs (lncRNAs) by qRT-PCR. Sub-cellular localization and association of lncRNAs and SARS-CoV-2 vRNA was depicted by Dual-fluorescence in situ hybridization (FISH), and immunostaining for epithelial cell markers. A 3D in-vitro cell culture model was developed using primary human RECs differentiated on transwells at air-liquid interface and were infected with a SARS-CoV-2 clinical isolate (USA-WI1/2020) via apical as well as basal regions. Samples were collected at 0, 1, 4, 24 and 48 h post-infection (p.i.) and the expression of aforementioned factors were analyzed in cell lysates and media. Results: Severely affected patients showed significantly higher expression of IL-6, ICAM-1, and CXCL-8 along with the respiratory mucins, MUC4, 5AC, 16, and 19 and the transcriptional regulators, SPDEF and FOXA3 compared to the mild COVID19 patients. Our recently identified novel lncRNAs, LASI (lncRNA on antisense strand to ICAM-1), and TOSL (TNFAIP3-opposite strand lncRNA) were significantly higher in severe patients whereas NEAT1 and MALAT1 levels were lower as were the ACE2 and TMPRSS2. SARS-CoV-2 clinical isolate productively infected 3D human REC model with highest expression of SARS-CoV-2 N vRNA at 24 h p.i., and showed increased expression of inflammatory factors and LASI and TOSL at 1 h p.i. The dual-FISH staining of LASI and SARS-CoV-2 N1 vRNA validated that both the transcripts were enriched in nuclear/perinuclear region of RECs and, club cells and MUC5AC+ cells of severe COVID19.Conclusions: Together, these data indicate that severely infected COVID19 patients are impacted by high respiratory mucin expression and the associated airway inflammation. Interestingly, the lncRNAs, LASI and TOSL showed associated increased expression suggesting a possible role for these innate immunomodulators in SARS-CoV-2 induced innate airway mucosal responses

Diversified Crop Rotation: An Approach for Sustainable Agriculture Production

Diversified crop rotation (DCR) improves the efficiency of farming systems all over the world. It has the potentiality to improve soil condition and boost system productivity. Improved soil attributes such as increased soil water uptake and storage, and a greater number of beneficial soil organisms, may improve yield tolerance to drought and other hard growing conditions in a variety of crop rotations. Crop rotations with a variety of crops benefit the farmers,reduce production risk and uncertainty, and enhance soil and ecological sustainability. Farmers may be able to diversify their sources of income by adopting diversified crop rotations. Furthermore, because of the distinct structure, function, and relationship of plant community with soil in DCR, it contributes to the long-term development of soil health by decreasing insect, weed, and disease incidence and increasing the physical and chemical structure of the soil. DCR is becoming more popular approach for maintaining sustainable crop production. This review provides the evidence of the significance of DCR, challenges to adapt it, and possible way out to overcome the challenges