Culture matters: using a cultural contexts of health approach to enhance policy-making

This is the final version of the report. Freely available online from WHO via the link in this recordThis policy brief has been developed in response to the increasing awareness among policy-makers and the public health community of the important relationship between culture and health. By exploring the three key public health areas of nutrition, migration and environment, the policy brief demonstrates how cultural awareness is central to understanding health and well-being and to developing more effective and equitable health policies. Consequently, it argues that public health policy-making has much to gain from applying research from the health-related humanities and social sciencesWorld Health Organisatio

Developmental regulation of nicotinic synapses on cochlear inner hair cells

In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits.Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Meson-exchange enhancement in first-forbidden β\beta -transitions: the case of 50^{50}K and 38^{38}Ca

The $\beta$- decay of $^{50}$K and $^{38}$Ca have been investigated with the main motive of determining more accurately the first-forbidden $\beta$- branches, in particular the rank-zero, $\Delta$J = 0, $\,\beta$ -transitions. $^{50}$K and $^{38}$Ca have been produced by fragmentation of U and Ti targets respectively, with a 1 GeV proton beam and subsequent on-line mass separation. For $^{50}$K, $\gamma$-ray spectroscopy, as well as delayed neutron spectroscopy by time of flight, were carried out to obtain a detailed decay scheme to 20 (bound and unbound) levels in $^{50}$Ca. The level structur e of $^{50}$Ca can be compared to recent calculations which incorporate 1p1h excitations from the f$_{7/2}$ shell. The first-forbidden $\beta^-$ transition $^{50}$ K(0$^-$)$\,\to{}^{50}$Ca(0$^+$) g.s. has been evaluated for the first time by a direct measurement of $\beta$- and $\gamma$- activities. Its importance (61.0 $\pm$ 7.4$\%$) is interpreted as an effect of the meson-exchange current (MEC) l eading to an enhancement factor of 62(5)$\%$ in comparison with the value predicted by shell-model calculations using the impulse approximation. For the $^{38}$ Ca$\,\to{}^{38}$K decay, chemical selec tive production was obtained through separation of the molecular ion CaF$^+$ without contamination by isobars. In these conditions, the measurement of very weak $\beta$-branches, at a level of 10$^{-3}\%$ decays, could be made and a limit, at the 2$\sigma$-confidence level, has been obtained for the 0$^+\to$ 0$^-$ branch to the level at E$_x$ = 2993 keV (I$_\beta$ < 0.0046$\%$). Imp lications of these results on the general trend of meson-exchange enhancements of first-forbidden transitions within the framework of the spherical shell model are discussed

Tinnitus Research: Improvement and Innovation

Until relatively recently it would have been justified to be critical of the standard of research into tinnitus. The sparse published literature was typified by studies with poor experimental design, low participant numbers, and research teams all from the same discipline. This situation has now undergone a transformation, with multidisciplinary research teams utilising modern neuroscience tools, in well designed and well powered studies. Clinical and neuroscientific perspectives are being brought to bear on tinnitus, and to place it within modern knowledge frameworks from imaging, auditory neuroscience, pharmacology, psychology, and medicine. This special issue of Trends in Hearing, entitled Innovations in Tinnitus Research, reflects the depth and breadth of the tinnitus field as it currently stands. The inception of the idea for the Special Issue was associated with the Tinnitus Research Initiative conference at the University of Regensburg, Germany in Spring 2018. This conference coincided with the closing conference of TINNET, a consortium of Europea

Ground-state spin of 59^{59}Mn

Beta decay of $^{59}$Mn has been studied at PSB-ISOLDE, CERN. The intense and pure Mn beam was produced using the Resonance Ionization Laser Ion Source (RILIS). Based on the measured $\beta$-decay rates the ground-state spin and parity are proposed to be $J^{\pi}$ = 5/2$^{-}$. This result is consistent with the systematic trend of the odd-A Mn nuclei and extends the systematics one step further towards the neutron drip line

Loss of the mammal-specific tectorial membrane component CEA cell adhesion molecule 16 (CEACAM16) leads to hearing impairment at low and high frequencies

The vertebrate-restricted carcinoembryonic antigen gene family evolves extremely rapidly. Among their widely expressed members, the mammal-specific, secreted CEACAM16 is exceptionally well conserved and specifically expressed in the inner ear. To elucidate a potential auditory function we inactivated murine Ceacam16 by homologous recombination. In young Ceacam16-/- mice the hearing threshold for frequencies below 10 kHz and above 22 kHz was raised. This hearing impairment progressed with age. A similar phenotype is observed in hearing-impaired members of Family 1070 with non-syndromic autosomal dominant hearing loss (DFNA4) who carry a missense mutation in CEACAM16. CEACAM16 was found in interdental and Deiters cells and was deposited in the tectorial membrane of the cochlea between postnatal day 12 and 15, when hearing starts in mice. In cochlear sections of Ceacam16-/- mice tectorial membranes were significantly more often stretched out as compared to wild-type mice where they were mostly contracted and detached from the outer hair cells. Homotypic cell sorting observed after ectopic cell surface expression of the carboxy-terminal immunoglobulin variable-like N2 domain of CEACAM16 indicated that CEACAM16 can interact in trans. Furthermore, Western blot analyses of membrane-bound CEACAM16 under reducing and non-reducing conditions demonstrated oligomerization via unpaired cysteines. Taken together, CEACAM16 probably can form higher order structures with other tectorial membrane proteins such as α-tectorin and β-tectorin and influences the physical properties of the tectorial membrane. Evolution of CEACAM16 might have been an important step for the specialization of the mammalian cochlea allowing hearing over an extended frequency range

Perforin deficiency attenuates collagen-induced arthritis

Collagen-induced arthritis (CIA), an approved animal model for rheumatoid arthritis, is thought to be a T cell-dependent disease. There is evidence that CD8(+ )T cells are a major subset controlling the pathogenesis of CIA. They probably contribute to certain features of disease, namely tissue destruction and synovial hyperplasia. In this study we examined the role of perforin (pfp), a key molecule of the cytotoxic death pathway that is expressed mainly in CD8(+ )T cells, for the pathogenesis of CIA. We generated DBA/1J mice suffering from mutations of the pfp molecule, DBA/1J-pfp(-/-), and studied their susceptibility to arthritis. As a result, pfp-deficient mice showed a reduced incidence (DBA/1J-pfp(+/+), 64%; DBA/1J-pfp(-/-), 54%), a slightly delayed onset (onset of disease: DBA/1J-pfp(+/+), 53 ± 3.6; DBA/1J-pfp(-/-), 59 ± 4.9 (mean ± SEM), and milder form of the disease (maximum disease score: DBA/1J-pfp(+/+), 7.3 ± 1.1; DBA/1J-pfp(-/-), 3.4 ± 1.4 (mean ± SEM); P < 0.05). Concomitantly, peripheral T cell proliferation in response to the specific antigen bovine collagen II was increased in pfp(-/- )mice compared with pfp(+/+ )mice, arguing for an impaired killing of autoreactive T cells caused by pfp deficiency. Thus, pfp-mediated cytotoxicity is involved in the initiation of tissue damage in arthritis, but pfp-independent cytotoxic death pathways might also contribute to CIA

Spectroscopy of 34,35Si^{34,35}Si by β\beta decay: sd-fp shell gap and single-particle states

The $^{34,35}Al\beta$ decays were studied at the CERN on-line mass separator ISOLDE by $\beta-\gamma, \beta-\gamma-\gamma$ and $\beta-n-\gamma$ measurements, in order to corroborate thelow-level description of $^{34}Si$ and to obtain the first information on the level structure of the N=21 isotope $^{35}Si$. Earlier observed $\gamma$ lines in $^{34} Al$ decay were confirmed and new gamma transitions following both beta decay and $\beta$-delayed neutron emission were established. The first level scheme in $^{35}Si$, including three excited states at 910, 974 and 2168 keV, is consistent with $J^{\pi} =3/2^{-}$ and $3/2^{+}$ for the first two states respectively. Beta-decay half-life of $T_{1/2} = 38.6 (4)$ ms and beta-delayed neutron branching $P_{n}$ value $(P_{n} =41(13)$ were measured unambiguously. The significance of the single-particle energy determination at N=21, Z=14, for assessing the effective interaction in sd-fp shell-model calculations, is discussed and illustrated by predictions for different n-rich isotopes

β\beta-decay half-life of 70^{70}Kr: a bridge nuclide for the rp-process beyond A = 70

The $\beta$-decay half-life of $^{70}$Kr has been measured for the first time at the ISOLDE PSB Facility at CERN. Mass separated $^{70}$Kr ions were produced by 1 GeV proton induced spallation reactions in a Nb foil. The measured half-life is 57(21) ms. This value is consistent with the half-life calculated assuming a pure Fermi decay, but is clearly lower than the value used in a recent rp-process reaction flow calculation. The result shows that the reaction flow via two-proton-capture of $^{68}$Se is 2.5 times faster than previously calculated assuming an astrophysical temperature of 1.5 GK and a density of 10$^{6}$g/cm$^{3}$