Factual Causation: The Missing Link in Hydraulic Fracture—Groundwater Contamination Litigation

A speech-based material that early can assess a child's hearing is needed to provide an indication of a hearing impaired child's language development. Being able to identify and discriminate between different sounds is a requirement for interpreting speech. Hearing assessments that examine speech perception can therefore, unlike, for example, pure tone audiometry, examine a hearing impaired child's perceptual language skills and provide a basis for intervention. The purpose of this work is to evaluate a new auditory material on 3-year-old children. The auditory material is based on the Ling-6-sounds and the main question was which of the sounds in the test were adequate for test of discrimination. Furthermore, it was investigated how the test works with children aged 3:0–3:11 years and how age and gender affect the result. Twenty-four children aged 3:0–3:11 years were tested using a computer based test. The goal  for the child was to discriminate between pairs of sounds and react to an odd pair. Several instruction techniques were tested by two different test administrators. The results of the tests indicated that equal pairs of sounds (/do/-/do/, /s/-/s/) gave more correct responses. The test section where only two different pairs were used generated more adequate responses than the test section with six different pairs. Differences in age influenced the outcome more than sex and different instructions. There is also a need for improved instructions and sounds. Generally, children became more involved in the test when given instructions that were short and direct

Selected Re-Emerging and Emerging Trends in Oil and Gas Law as a Result of Production From Shale Formations

Taking the statements in the above paragraph as true, there is also another consequence to these developments. There has been a proliferation of oil and gas leasing and drilling in areas that have little prior experience, or little recent experience, with such activities. This has caused a resurgence of oil and gas law and raised issues that have been either dormant or nonexistent. This Article will address some of the re-emerging and emerging oil and gas litigation issues that are the byproduct of shale development activities. Specifically, this Article will discuss: 1. Production in paying quantities and shut-in royalties; 2. Oil and gas lease drafting, and in particular, the use of termination clauses; 3. The impact of the Uniform Electronic Transactions Act on oil and gas law; and 4. Allegations of groundwater contamination caused by hydraulic fracture stimulation

Reactivity Control Schemes for Fast Spectrum Space Nuclear Reactors

Several different reactivity control schemes are considered for future space nuclear reactor power systems. Each of these control schemes uses a combination of boron carbide absorbers and/or beryllium oxide reflectors to achieve sufficient reactivity swing to keep the reactor subcritical during launch and to provide sufficient excess reactivity to operate the reactor over its expected 7-15 year lifetime. The size and shape of the control system directly impacts the size and mass of the space reactor\u27s reflector and shadow shield, leading to a tradeoff between reactivity swing and total system mass. This paper presents a trade study of drum, shutter, and petal control schemes based on reactivity swing and mass effects for a representative fast-spectrum, gas-cooled reactor. For each control scheme, the dimensions and composition of the core are constant, and the reflector is sized to provide $5 of cold-clean excess reactivity with each configuration in its most reactive state. The advantages and disadvantages of each configuration are discussed, along with optimization techniques and novel geometric approaches for each scheme

Philosophy of Language

Names, pronouns, indexicals, and demonstratives (including complex demonstratives-e.g. 'that dog') are often conventionally used to designate individuals. 2 For ease of exposition, let's mark this point by calling such expressions singular terms, not intending by so doing to prejudge the question of whether they have anything interesting in common semantically. Some uses of each kind of singular term at least appear to semantically refer to the individuals designated, and so contribute them to propositions expressed (in the contexts in question) by the sentences they occur in. But each kind of singular term has other uses that don't seem to be referring uses at all; that is, the uses don't appear to contribute individuals to propositions. The primary question that is the concern of this paper is: what should be our theoretical reaction to the existence of what appear to be these semantically quite different uses of singular terms? For various reasons, I propose to set aside the case of pronouns here. Thus, I propose to concentrate on the other cases mentioned: the cases of names, indexicals and complex demonstratives. I take it that one can easily imagine uses of names, indexicals and complex demonstratives on which the uses seem to refer to the individuals they designate, and hence contribute the individuals to the propositions expressed. Such uses are those that are most discussed in the semantics literature. For the sake of explicitness, let's consider some paradigmatic examples of uses of this sort. Names (uttered by me talking about a good friend I have known for many years) 1. Glenn is a good architect. Indexical

Cooperative effects in two-dimensional ring-like networks of three-center hydrogen bonding interactions

Cooperative effects in two-dimensional cyclic networks containing intermolecular three-centered hydrogen bonding interactions of the type H1&#;A&#;H2 are investigated by means of ab intio molecular orbital and density functional theory calculations. Ring-like clusters consisting of three and up to nine monomers of the cis–cis isomer of carbonic acid H2CO3 are used as basic models, where each unit acts simultaneously as a double hydrogen-bond donor and double hydrogen-bond acceptor. Cooperative effects based on binding energies are evident for (H2CO3)n, where n goes from 2 to 9. Thus, the ZPVE-corrected dissociation energy per bifurcated hydrogen bond increases from 11.52 kcal/mol in the dimer to 20.42 kcal/mol in the nonamer, i.e., a 77% cooperative enhancement. Cooperative effects are also manifested in such indicators as geometries, and vibrational frequencies and intensities. The natural bond orbital analysis method is used to rationalize the results in terms of the substantial charge delocalization taking place in the cyclic clusters. Cooperativity seems close to reaching an asymptotic limit in the largest ring considered, n=9

Increase in Mitochondrial content after Electrical Pulse Stimulation is dependent on duration of stimulation

Increase in Mitochondrial content after Electrical Pulse Stimulation is dependent on duration of stimulation Daniel Conde B.S.1, Jeffrey D. Covington Ph.D.2, Cecilia Gamboa3 George A. King Ph.D.1, Arild C. Rustan Ph.D.4, Sudip Bajpeyi Ph.D.1. 1 Department of Kinesiology, University of Texas at El Paso, TX; 2Louisiana State University Health Sciences Center, New Orleans, LA; 3Clinical Laboratory Sciences, University of Texas at El Paso; 4Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway. Skeletal Muscle Metabolism Laboratory; Kinesiology; University of Texas at El Paso; El Paso, TX Category: Masters Advisor / Mentor: Bajpeyi, Sudip ([email protected]) ABSTRACT We have previously shown that human skeletal muscle myotubes cultured in vitro, retain in vivo characteristics of the donors. Recent studies indicate that electrical pulse stimulation (EPS) can be used as an exercise mimetic in a cell culture model, and could be beneficial to understand molecular mechanisms underlying exercise training. Purpose: The purpose of this study was to compare acute, moderate and long duration EPS treatments on mitochondrial and lipid content in cultured myotubes. Methods: EPS stimulation was applied to human myotubes cultured from sedentary donors under three conditions: Acute (bipolar pulses of 100 Hz for 200 ms every 5th second; 30V for 60 min) and chronic stimulation (single bipolar pulses of 2 ms; 30V, 1Hz continuously for 24 h or 48 h). Mitochondrial and lipid contents were measured by primary antibody for complex IV and bodipy green dye, respectively, using immunohistochemistry techniques. Fluoroskan ascent microplate reader was used to quantify fluorescence signals. OXPHOS proteins were measured using western immunoblotting. Results: There was no change in lipid or mitochondrial content as assessed by immunohistochemistry after acute EPS stimulation. Chronic stimulation resulted in a significant increase in the mitochondrial content after 24 h (from 0.183 ± 0.02 AU to 0.350 ± 0.03 AU; p=0.008) and 48 h (from 0.290 ± 0.01 AU to 0.337 ± 0.01 AU; p=0.02) of continuous EPS stimulation. OXPHOS proteins increased after 48 h of EPS. There was also a significant increase in lipid content after 48 h of EPS stimulation (from 0.210 ± 0.01 AU to 0.256 ± 0.01 AU; p=0.02). Conclusion: These findings suggest that 48 h of chronic EPS results in an increase in both mitochondrial and lipid contents in human myotubes. The concomitant increase in lipid and mitochondrial content after exercise mimetic EPS stimulation supports the elevated level of intramyocellular lipid and mitochondrial content evident in endurance trained athletes

The protective effects of plasma gelsolin on stroke outcome in rats

<p>Abstract</p> <p>Background</p> <p>To date, recombinant tissue plasminogen activator (rtPA) is the only approved drug for ischemic stroke. It is intravenously administered functioning as a thrombolytic agent and is used to obtain reperfusion of the affected area of the brain. Excitotoxicity, inflammation and apoptosis are all involved in delayed neuronal death following stroke and offer multiple opportunities to intervene with neuroprotective agents. Gelsolin (GSN) is an actin- and calcium-binding protein mediating the disassembly of actin filaments and activity of calcium channels. It also functions as a regulator of apoptosis and inflammatory responses. This study tests the hypothesis that increasing the concentration of the form of GSN known as plasma GSN (pGSN) near an infarct will provide neuroprotection following ischemic stroke.</p> <p>Methods</p> <p>We induced middle cerebral artery occlusion (MCAO) in male rats via intracranial injection of endothelin-1 (ET-1), a potent vasoconstrictor, and then treated with local delivery of pGSN. Whole brain laser Doppler perfusion imaging was performed through the skull to assess MCAO effectiveness. Cylinder and vibrissae tests evaluated sensorimotor function before and 72 h after MCAO. Infarct volumes were examined 72 h after MCAO via 2, 3, 5-triphenyltetrazolium chloride (TTC) assay.</p> <p>Results</p> <p>Estimates of relative cerebral perfusion were significantly decreased in all groups receiving MCAO with no differences detected between treatments. Despite equivalent initial strokes, the infarct volume of the pGSN treatment group was significantly reduced compared with the untreated MCAO rats at 72 h. ET-1 induced significant deficits in both cylinder and vibrissae tests while pGSN significantly limited these deficits.</p> <p>Conclusion</p> <p>Gelsolin could be a promising drug for protection against neurodegeneration following ischemic stroke.</p

Nitropyrene: DNA binding and adduct formation in respiratory tissues.

Binding of 1-nitro (14C)pyrene (NP) or its metabolites to cellular DNA and protein in cultures of rabbit alveolar macrophages, lung tissue, and tracheal tissue was examined. DNA binding in tracheal tissue (136 +/- 18.3 pmole NP/mg DNA) was four to five times the levels measured in either lung tissue (38 +/- 9.4 pmole NP/mg DNA) or macrophages (26 +/- 7.5 pmole NP/mg DNA). Adduct analysis of DNA isolated from lung tissue incubated with 1-nitro[H3]pyrene in vitro resulted in the identification of 2 to 5% of the NP adducts as C8-deoxyguanosine 1-aminopyrene. NP was also bound to cellular protein in tracheal tissue and lung tissue, and at a lower level in macrophages. Cocultivation of the macrophages with lung and tracheal tissue decreased the DNA binding in tracheal tissue by 45%. Following intratracheal instillation of diesel particles (5 mg) vapor-coated with 14C-NP (380 ppm, 0.085 muCi/mg) particles into rats, 5-8% of the radioactivity remained in the lungs after 20 hr. Most of the diesel particles were also deposited in the lung. Examination of DNA and protein binding in this tissue showed 5 to 12% of the pulmonary 14C bound to protein and no detectable levels of 14C bound to DNA

Subspace Correction for Constraints

We demonstrate that it is possible to construct operators that stabilize the constraint-satisfying subspaces of computational problems in their Ising representations. We provide an explicit recipe to construct unitaries and associated measurements for some such constraints. The stabilizer measurements allow the detection of constraint violations, and provide a route to recovery back into the constrained subspace. We call this technique ``subspace correction". As an example, we explicitly investigate the stabilizers using the simplest local constraint subspace: Independent Set. We find an algorithm that is guaranteed to produce a perfect uniform or weighted distribution over all constraint-satisfying states when paired with a stopping condition: a quantum analogue of partial rejection sampling. The stopping condition can be modified for sub-graph approximations. We show that it can prepare exact Gibbs distributions on $d-$regular graphs below a critical hardness $\lambda_d^*$ in sub-linear time. Finally, we look at a potential use of subspace correction for fault-tolerant depth-reduction. In particular we investigate how the technique detects and recovers errors induced by Trotterization in preparing maximum independent set using an adiabatic state preparation algorithm.Comment: 12 + 4 pages, 6 figure