Evaluation of the genetic structure of the urban dwelling species of Bank Myna (Acridotheres ginginisnus) using random amplified polymorphic DNA (RAPD) analysis

We used the random amplified polymorphic DNA (RAPD) technique to decipher the genetic structure of the Bank Myna (Acridotheres ginginisnus) in Pakistan. The samples were collected from four cities namely: Dera Ghazi Khan, Jahanian, Khanewal and Gujranwala. The analysis showed a high genetic diversity at species level (H = 0.318, S = 0.467) but low levels at population levels (H = 0.047, S = 0.069 to H = 0.195, S = 0.283). The four populations analyzed were genetically distant from each other and hinted the effect of urbanization role in isolating the urban dwelling species. The genetic distances between populations ranged from D = 0.2403 to D = 0.3419 and the similarity coefficient showed low range from 0.66 to 0.70. This study will help in future conservation plans and also help the understanding of the role of urbanization on fragmentation of species’ natural distribution and dispersal mechanism.Key words: Acridotheres ginginisnus, Bank Myna, random amplified polymorphic DNA (RAPD), genetic structure

Population genetic structure of Rufous-Vented Prinia (Prinia burnesii) in Pakistan

The objective of the study is to ascertain genetic variation within Rufous-vented Prinia, Prinia burnesii an endemic species, by DNA fingerprinting applying random amplified polymorphic DNA (RAPD) technique. Genetic material was obtained from three distant sites along western bank of River Indus. These sites include Chashma barrage (32°50’N, 71°20’E), in the north; Taunsa barrage (30°45’N, 70°45’E) and the southern population from Guddu barrage (28°26’N, 69°44’E). In total, 14 RAPD primers were selected to determine the genetic variability between three populations of the species. The Nei’s (1973) genetic diversity in three populations ranged from 0.190 to 0.320. The genetic distance between Populations ranged from 0.149 to 0.265. The polymorphism levels ranged from 48 to 83% in three populations and at species level, polymorphism were 93.07%. The results further indicate that they are genetically isolated populations.Keywords: Random Amplified Polymorphic DNA, Prinia, genetic variation, river Indu

Cloning and expression of Toxoplasma gondii tachyzoite P22 protein

Delay in diagnosis of Toxoplasma gondii infection in pregnant women who have been infected during the first trimester of gestation can lead to death of her fetus. Serological tests based on recombinant proteins are the main diagnosis methods for the detection of anti Toxoplasma antibody in serum samples. The aim of this study was to clone and express a gene encoding a P22 protein of T. gondii tachyzoite as using antigen for ELISA serology method. DNA was extracted from T. gondii (RH-strain) tachyzoites and PCR reaction was done using corresponding primers. The PCR product was purified, ligated to PTZ57R plasmid via T/A cloning method and subcloned into SacI and BamHI digested pET32a expression vector. Recombinant plasmid was transformed in E. coli (Bl21 DE3) and induced by 1 mM IPTG and analyzed by 12% SDS-PAGE. Expressd protein was purified by affinity chromatography and confirmed by western blot analysis. We successfully cloned and expressed T. gondii P22 protein.Key words: Toxoplasma gondii, cloning, recombinant P22

Perceived transaction cost and its antecedents associated with fintech users' intention: Evidence from Pakistan

Researches depict a considerable degree of acknowledgment of financial technology improvement in Pakistan. However, the costs blocking clients' intention to utilize financial technology remain dubious. Building upon Transaction Cost Economics and Innovation diffusion theory, this paper hypothesizes that consumers' transaction cost of fintech is affected by nine antecedents: perceived asset specificity, complexity, product uncertainty, behavioral uncertainty, transaction frequency, dependability, limitations, convenience, and economic utility. Transaction cost has a negative relationship with consumers' intentions to use fintech for online buying or availing services. We tested the model using data gathered from the individuals. The results show that factors that are positively related to consumers' perceived transaction cost, among them product uncertainty (β = 0.231) is the greatest of factors, followed by behavior uncertainty (β = 0.209) and asset specificity (β = 0.17), those that are negatively associated are dependability (β = 0.11), and convenience (β = 0.224). The study is limited in scope, focusing primarily on cost factors. Future research may analyze additional cost-related elements and the actual use of financial technology by using samples from different countries. © 2023Xi'an Eurasia University, EU; Ministry of Science and Higher Education of the Russian Federation: 018/RID/2018/19, PLN 10 788 423,16H5 determines whether perceived transaction frequency has a negative relationship with cost. According to the results (PTF - > PTC, β = -0.031, t = 0.853, P = 0.394), this hypothesis is not supported.H6 is whether there is a negative relationship between the dependability of fintech and perceived transaction cost. The results (DPND - > PTC, β = -0.11, t = 2.433, P = 0.015) support this hypothesis.H8 states a negative relationship between convenience and perceived transaction cost. The results (C - > PTC, β = -0.224, t = 5.456, P = 0) support this hypothesis.H9 states a negative relationship between economic utility and perceived transaction cost. However, findings (EU - > PTC, β = 0.02, t = 0.605, P = 0.546) show that this hypothesis is not supported.The project was funded under the program of the Minister of Science and Higher Education titled “Regional Initiative of Excellence” in 2019–2022, project number 018/RID/2018/19, the amount of funding PLN 10 788 423,16

Analysis of streamflow characteristics over Northeastern Canada in a changing climate

An analysis of streamflow characteristics (i.e. mean annual and seasonal flows and extreme high and low flows) in current and future climates for 21 watersheds of north-east Canada covering mainly the province of Quebec is presented in this article. For the analysis, streamflows are derived from a 10-member ensemble of Canadian Regional Climate Model (CRCM) simulations, driven by the Canadian Global Climate Model simulations, of which five correspond to current 1970–1999 period, while the other five correspond to future 2041–2070 period. For developing projected changes of streamflow characteristics from current to future periods, two different approaches are used: one based on the concept of ensemble averaging while the other approach is based on merged samples of current and similarly future simulations following multiple comparison tests. Verification of the CRCM simulated streamflow characteristics for the 1970–1999 period suggests that the model simulated mean hydrographs and high flow characteristics compare well with those observed, while the model tends to underestimate low flow extremes. Results of projected changes to mean annual streamflow suggest statistically significant increases nearly all over the study domain, while those for seasonal streamflow show increases/decreases depending on the season. Two- and 5-year return levels of 15-day low flows are projected to increase significantly over most part of the study domain, though the changes are small in absolute terms. Based on the ensemble averaging approach, changes to 10- and 30-year return levels of high flows are not generally found significant. However, when a similar analysis is performed using longer samples, significant increases to high flow return levels are found mainly for northernmost watersheds. This study highlights the need for longer samples, particularly for extreme events in the development of robust projections

Can retail investors induce corporate green innovation? -Evidence from Baidu Search Index

China's rapid economic development has caused some environmental damage in recent years. The popularity of the Internet has enriched the ways for investors to obtain information, which would exert an impact on corporate environmental behavior. Focusing on micro-enterprise green innovation from the perspective of informal regulation, this paper investigates the impact of investor attention on corporate green innovation. This study takes Chinese A-share listed companies from 2011 to 2018 as samples, constructs panel fixed-effects models and adopts multiple linear, Logistic and Tobit regressions. This article finds that investor attention, measured by the web search index, can significantly improve corporate green innovation. The conclusion is still valid after a series of robust tests. Besides, mechanism tests reveal that investor attention can promote corporate green innovation by improving the implementation efficiency of punitive environmental regulation, the use efficiency of environmental subsidies, and by increasing the reputation cost of enterprises. In additional tests, this paper further clarifies that investors' attention to negative public opinion can play a better role in environmental governance, and reveals the reason why investors are motivated to improve corporate green innovation. This research puts forward a unique perspective, which extends the understanding of informal environmental regulation and enriches research on green innovation at the micro-enterprise level, promoting the cross research of finance and environmental protection. © 2022 The Author(s

Novel Carbyne Filled Carbon Nanotube – Polymer Nanocomposites

The availability of carbyne in carbon nanotubes (CNTs) induces intrinsic stiffening and strengthening of CNTs, and is exploited for the very first time in this report to process epoxy nanocomposites with improved mechanical and electrical properties. The existence of encapsulated carbyne in double wall CNTs (DWNTs) was confirmed using High Resolution Transmission Electron Microscopy (HR-TEM). The intrinsic stiffening of carbyne reinforced DWNTs (c-DWNTs) in epoxy matrix was visually confirmed by Field Emission Scanning Electron Microscopy (FE-SEM). In comparison to raw DWNTs reinforced epoxy nanocomposites, c-DWNTS imparted modest but improved tensile strength (5.6%), elastic modulus (9.7%), failure strain (9.9%) and fracture toughness (13%) to their respective epoxy nanocomposites. This inaugural study on carbyne-filled polymer composites also reports a minor but distinct increase (an order of magnitude) in the electrical conductivity for c-DWNTs filled epoxy nanocomposites compared to DWNT filled epoxy nanocomposites

A roadmap to develop dementia research capacity and capability in Pakistan: a model for low- and middle-income countries

Objective To produce a strategic roadmap for supporting the development of dementia research in Pakistan. Background While global research strategies for dementia research already exist, none is tailored to the specific needs and challenges of low- and middle-income countries (LMIC) like Pakistan. Methods We undertook an iterative consensus process with lay and professional experts to develop a Theory of Change-based strategy for dementia research in Pakistan. This included Expert Reference Groups (ERGs), strategic planning techniques, a “research question” priority survey, and consultations with Key Opinion Leaders. Results We agreed on ten principles to guide dementia research in Pakistan, emphasizing pragmatic, resource sparing, real-world approaches to support people with dementia, both locally and internationally. Goals included capacity/capability building. Priority research topics included raising awareness and understanding of dementia, and improving quality of life. Conclusion This roadmap may be a model for other LMIC health ecosystems with emerging dementia research cultures