Konsepsi Penggantian Kerugian Atas Pemberian Izin Mendirikan Bangunan (Imb) yang Tidak Sesuai dengan Rtrw (Kajian terhadap Pasal 37 Undang-undang No.26 Tahun 2007 Tentang Penataan Ruang)

Pasal 37 Ayat (4),(5) dan (8) Undang-undang No.26 Tahun 2007 Tentang Penataan Ruang mengatakan bahwa IMB harus mengikuti konsep perencanaan yang tertera pada Rencana Tata Ruang Wilayah (RTRW) di setiap daerah dan apabila diketahui IMB tersebut melanggar RTRW maka harus dibatalkan dan dimungkinkan adanya pemberian ganti rugi atas pembatalan IMB tersebut. Fenomena yang terjadi saat ini adalah belum jelas dan belum konkretnya aturan yang ada terkait dengan konsepsi ganti rugi sehingga menyulitkan pihak-pihak yang ingin mengajukan upaya hukum melalui sarana hukum yang paling tepat dan efisien. Berdasarkan penelitian ini, penulis menawarkan sarana hukum administrasi karena dianggap yang paling efektif dan jelas dalam menyelesaikan permasalahan IMB yang dilakukan pembatalan, dikarenakan IMB merupakan Keputusan Tata Usaha Negara (KTUN) yang apabila bermasalah sudah terakomodasi di Pengadilan Tata Usaha Negara, sesuai dengan kompetensinya dan yang paling penting adalah gugatan yang dilakukan, terhadap subjek kewenangan yaitu pejabatnya bukan pribadi dari pejabat tersebut yang bertanggung jawab terhadap kesalahan-kesalahan yang berkaitan dengan ketidaksesuaiannya IMB dengan RTRW. Maka, penulis mengusulkan konsepsi penggantian atas kerugian yang diderita oleh investor atau masyarakat dengan melalui mekanisme penggantian yang dibebankan pada pemerintah daerah melalui Anggaran Pendapatan dan Belanja Daerah (APBD). Sehingga diharapkan dapat mengembalikan hakikat tujuan dan manfaat dari IMB.Kata Kunci : Izin Mendirikan Bangunan (IMB), Rencana Tata Ruang Wilayah (RTRW), Upaya Hukum Administrasi, Ganti Rugi

Determination of etoposide serum concentrations in small pediatric samples by an improved method of reversed-phase high-performance liquid chromatography.

Several specific assays have been developed for the measurement of etoposide in biological fluids. As large samples are required for high sensitivity, these systems are not appropriate for a pediatric practice. In the present study, however, an improved method for the determination of serum levels of the anticancer drug etoposide was developed, using high-performance liquid chromatography with fixed-wavelength ultraviolet detection. Etoposide was extracted from serum using dichloromethane. The efficiency of extraction from serum was 85.7 +/- 7.7% for etoposide and 81.1 +/- 8.4% for diphenylhydantoin, the internal standard. The serum concentrations of etoposide were measured in 0.2-ml serum samples. The lower limit of detection was 50 ng/ml. Each measurement was completed within 5 min. The linear quantitation range for etoposide was 0.05-50 microg/ml. This assay presents an alternative method for routine measurement of serum levels of etoposide in the pediatric oncology setting.</p

Diradicals Produce Ambipolar Transistors: What and Why

organic conjugated diradicals have gaining interest in the recent times given their uses in different formats of electronic applications encompassing OFETs,1 non-linear optical dyes, chromophores for singlet fission in OPV,2 etc. Our recent interests have turned into the role of their open-shell structures and their impact in the stabilization of +1, -1 charges. Since the first studies in fused planar diradicals, we were fascinated by the fully reversible and almost perfect oxidation/reduction mirror-like shapes of their cyclic voltammetries (i.e., uncommon in the organic field, in Fig. 1).3 This aimed us to test the ambipolar charge transport character of diradicals in OFETs and discovered that some particular diradicals are able to transport both kind of charges, holes and electrons, with similar mobility, or balanced p-n mobility.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane

Protein phosphorylation is a fundamental post-translational modification in all organisms. In photoautotrophic organisms, protein phosphorylation is essential for the fine-tuning of photosynthesis. The reversible phosphorylation of the photosystem II (PSII) core and the light-harvesting complex of PSII (LHCII) contribute to the regulation of photosynthetic activities. Besides the phosphorylation of these major proteins, recent phosphoproteomic analyses have revealed that several proteins are phosphorylated in the thylakoid membrane. In this study, we utilized the Phos-tag technology for a comprehensive assessment of protein phosphorylation in the thylakoid membrane of Arabidopsis. Phos-tag SDS-PAGE enables the mobility shift of phosphorylated proteins compared with their non-phosphorylated isoform, thus differentiating phosphorylated proteins from their non-phosphorylated isoforms. We extrapolated this technique to two-dimensional (2D) SDS-PAGE for detecting protein phosphorylation in the thylakoid membrane. Thylakoid proteins were separated in the first dimension by conventional SDS-PAGE and in the second dimension by Phos-tag SDS-PAGE. In addition to the isolation of major phosphorylated photosynthesis-related proteins, 2D Phos-tag SDS-PAGE enabled the detection of several minor phosphorylated proteins in the thylakoid membrane. The analysis of the thylakoid kinase mutants demonstrated that light-dependent protein phosphorylation was mainly restricted to the phosphorylation of the PSII core and LHCII proteins. Furthermore, we assessed the phosphorylation states of the structural domains of the thylakoid membrane, grana core, grana margin, and stroma lamella. Overall, these results demonstrated that Phos-tag SDS-PAGE is a useful biochemical tool for studying in vivo protein phosphorylation in the thylakoid membrane protein

Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

<p>Abstract</p> <p>Background</p> <p>We performed an environmental study of viruses infecting the symbiotic single-celled algae of <it>Paramecium bursaria </it>(<it>Paramecium bursaria Chlorella </it>virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all <it>Chlorella variabilis </it>virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization.</p> <p>Results</p> <p>CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the <it>dnapol </it>gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A.</p> <p>Conclusions</p> <p>We conclude that CvV-BW1 composes a distinct species within <it>C. variabilis </it>virus.</p

Improved Adsorption of an Enterococcus faecalis Bacteriophage ΦEF24C with a Spontaneous Point Mutation

Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce the efficacy of conventional antimicrobial chemotherapy. Thus, the introduction of bacteriophage (phage) therapy is expected, where a phage is used as a bioagent to destroy bacteria. E. faecalis phage ΦEF24C is known to be a good candidate for a therapeutic phage against E. faecalis. However, this therapeutic phage still produces nonuniform antimicrobial effects with different bacterial strains of the same species and this might prove detrimental to its therapeutic effects. One solution to this problem is the preparation of mutant phages with higher activity, based on a scientific rationale. This study isolated and analyzed a spontaneous mutant phage, ΦEF24C-P2, which exhibited higher infectivity against various bacterial strains when compared with phage ΦEF24C. First, the improved bactericidal effects of phage ΦEF24C-P2 were attributable to its increased adsorption rate. Moreover, genomic sequence scanning revealed that phage ΦEF24C-P2 had a point mutation in orf31. Proteomic analysis showed that ORF31 (mw, 203 kDa) was present in structural components, and immunological analysis using rabbit-derived antibodies showed that it was a component of a long, flexible fine tail fiber extending from the tail end. Finally, phage ΦEF24C-P2 also showed higher bactericidal activity in human blood compared with phage ΦEF24C using the in vitro assay system. In conclusion, the therapeutic effects of phage ΦEF24C-P2 were improved by a point mutation in gene orf31, which encoded a tail fiber component

Use of Recombinant Endolysin to Improve Accuracy of Group B Streptococcus Tests

Group B Streptococcus (GBS) causes serious neonatal infection via vertical transmission. The prenatal GBS screening test is performed at the late stage of pregnancy to avoid risks of infection. In this test, enrichment culture is performed, followed by GBS identification. Selective medium is used for the enrichment; however, Enterococcus faecalis, which is a potential contaminant in swab samples, can interfere with the growth of GBS. Such bacterial contamination can lead to false-negative results. Endolysin, a bacteriophage-derived enzyme, degrades peptidoglycan in the bacterial cell wall; it is a promising antimicrobial agent for selectively eliminating specific bacterial genera/species. In this study, we used the recombinant endolysin EG-LYS, which is specific to E. faecalis; the endolysin potentially enriched GBS in the selective culture. First, in the false-negative model (coculture of GBS and E. faecalis, which disabled GBS detection in the subsequent GBS identification test), EG-LYS treatment at 0.1 mg/ml improved GBS detection. Next, we used 548 vaginal swabs to test the efficacy of EG-LYS treatment in improving GBS detection. EG-LYS treatment (0.1 mg/ml) increased the GBS-positive ratio to 17.9%, compared to 15.7% in the control (phosphate-buffered saline [PBS] treatment). In addition, there were an increased number of GBS colonies under EG-LYS treatment in some samples. The results were supported by the microbiota analysis of the enriched cultures. In conclusion, EG-LYS treatment of the enrichment culture potentially improves the accuracy of the prenatal GBS screening test

Mini-TCRs: Truncated T cell receptors to generate T cells from induced pluripotent stem cells

Allogeneic T cell platforms utilizing induced pluripotent stem cell (iPSC) technology exhibit significant promise for the facilitation of adoptive immunotherapies. While mature T cell receptor (TCR) signaling plays a crucial role in generating T cells from iPSCs, the introduction of exogenous mature TCR genes carries a potential risk of causing graft-versus-host disease (GvHD). In this study, we present the development of truncated TCRα and TCRβ chains, termed mini-TCRs, which lack variable domains responsible for recognizing human leukocyte antigen (HLA)-peptide complexes. We successfully induced cytotoxic T lymphocytes (CTLs) from iPSCs by employing mini-TCRs. Combinations of TCRα and TCRβ fragments were screened from mini-TCR libraries based on the surface localization of CD3 proteins and their ability to transduce T cell signaling. Consequently, mini-TCR-expressing iPSCs underwent physiological T cell development, progressing from the CD4 and CD8 double-positive stage to the CD8 single-positive stage. The resulting iPSC-derived CTLs exhibited comparable cytokine production and cytotoxicity in comparison to that of full-length TCR-expressing T lymphocytes when chimeric antigen receptors (CARs) were expressed. These findings demonstrate the potential of mini-TCR-carrying iPSCs as a versatile platform for CAR T cell therapy, offering a promising avenue for advancing adoptive immunotherapies

A Terrestrial SER Estimation Methodology based on Simulation coupled with One-Time Neutron Irradiation Testing

電子機器の信頼性評価の迅速化に光明 --様々な中性子施設で半導体ソフトエラー評価を可能にする技術を開発--. 京都大学プレスリリース. 2023-06-08.Terrestrial soft error rates (SERs) are generally estimated by performing an experiment using spallation neutron beam with the energy spectrum being similar to that of the terrestrial neutrons or at least four measurements using various (quasi-)mono-energetic neutron and/or proton sources to determine the parameters of the Weibull function. We here propose a method to estimate the terrestrial SERs based on simulation coupled with one-time neutron irradiation testing which can be applied to various kinds of neutron sources. In this method, the dependences of single event upset (SEU) cross sections on the neutron energy and the critical charge are calculated by simulation using Particle and Heavy Ion Transport code System (PHITS). The critical charge is used as the only calibration parameter, which is adjusted to reproduce the SER measured by one-time neutron irradiation. The validity of our method is investigated for 65-nm bulk SRAMs with the measured data using various neutron sources in Japan. Our method generally provides the reasonable terrestrial SERs compared with those obtained by the Weibull function method. This result indicates the feasibility of evaluating the terrestrial SER using one of the various neutron sources available all over the world, including those not dedicated to SER measurement. We also investigate the necessity of the elaborated geometry of device under test (DUT) for the accuracy of the simulation. It is shown that detailed material compositions of DUT are not necessary in our method except when the one-time irradiation is performed using the neutron source that contains a high-quantity of low-energy neutrons below 8 MeV. Furthermore, we confirm that the configuration of the sensitive volume can be simplified without sacrificing the estimation accuracy. These simplifications in the simulation help to reduce the modeling and calculation cost in SER estimation

Association between mitral annulus calcification and subtypes of heart failure rehospitalization

Background: Mitral annulus calcification (MAC) has been associated with cardiovascular diseases, including heart failure (HF); however, the associations between MAC and both the category and etiology of HF have not been fully elucidated. The aim of this study was to investigate the relationship between MAC and three types of HF rehospitalization: HF with preserved ejection fraction (HFpEF), HF with mid-range EF (HFmrEF), and HF with reduced EF (HFrEF). Methods: We enrolled consecutive patients undergoing echocardiography, who were admitted to our hospital for clinically indicated congestive HF between April 2014 and March 2018. Cox proportional-hazards models were used after adjusting for age, gender, and hypertension. Results: Of 353 patients, 40 (11.3%) had MAC. With a median follow-up of 2.8 years, 100 (28%) patients were rehospitalized for congestive HF (HFpEF 40%, HFmrEF 16%, HFrEF 44%, respectively). According to the Kaplan-Meier method, the estimated incidence of HFpEF rehospitalization in the MAC group was significantly greater than that in the non-MAC group (p &lt; 0.001) whereas the incidences of HFmrEF and HFrEF rehospitalization were comparable between the groups (p = 0.101 and p = .291, respectively). In a multivariate analysis, MAC remained significantly associated with HFpEF rehospitalization (hazard ratio: 3.379; 95% confidence interval: 1.651–6.597). At initial HF hospitalization, E/e’ was significantly higher in the MAC group (both septum and lateral, p &lt; 0.05), suggesting a possible relationship between MAC and left ventricular diastolic function. Conclusions: Mitral annulus calcification was associated with increased HFpEF rehospitalization and might be a cause of left ventricular diastolic dysfunction