Effect of buffer on heparin binding and sensing in competitive aqueous media

Although buffer-specific effects on molecular recognition are known in biological science, they remain rare in supramolecular chemistry. The binding between a cationic dye, Mallard Blue (MalB), and polyanionic heparin in aqueous NaCl (150 mM) is studied in three commonly-used buffers (Tris-HCl, HEPES, Phosphate, each 10 mM). Although MalB has a very similar UV-Vis spectrum in each buffer, the sensory response towards heparin was different in each case. This can be ascribed to differences in the complex formed. In Tris-HCl which has the least competitive chloride counter-anions, MalB exhibits a hypsochromic shift of 25 nm, assigned to strong binding and aggregation of the dye on heparin. In more competitive HEPES, containing a sulfonate anion, there is weaker binding and less aggregation of MalB along the heparin; the hypsochromic shift is only 15 nm. In phosphate buffer, MalB can interact quite strongly with buffer phosphate anions; although heparin binding is still observed, the hypsochromic shift associated with dye aggregation is only 5 nm. As such, specific buffer interactions with the MalB-heparin complex mediate host-guest binding and sensing. Buffer choice must be made carefully in studies of molecular recognition – we would caution against using phosphate and sulfonate containing buffers when studying electrostatic binding

Impact of lightning-NO on eastern United States photochemistry during the summer of 2006 as determined using the CMAQ model

A lightning-nitrogen oxide (NO) algorithm is implemented in the Community Multiscale Air Quality Model (CMAQ) and used to evaluate the impact of lightning-NO emissions (LNO<sub>x</sub>) on tropospheric photochemistry over the United States during the summer of 2006. <br><br> For a 500 mole per flash lightning-NO source, the mean summertime tropospheric NO<sub>2</sub> column agrees with satellite-retrieved columns to within −5 to +13%. Temporal fluctuations in the column are moderately well simulated; however, the addition of LNO<sub>x</sub> does not lead to a better simulation of day-to-day variability. The contribution of lightning-NO to the model column ranges from ∼10% in the northern US to >45% in the south-central and southeastern US. Lightning-NO adds up to 20 ppbv to upper tropospheric model ozone and 1.5–4.5 ppbv to 8-h maximum surface layer ozone, although, on average, the contribution of LNO<sub>x</sub> to model surface ozone is 1–2 ppbv less on poor air quality days. LNO<sub>x</sub> increases wet deposition of oxidized nitrogen by 43% and total deposition of nitrogen by 10%. This additional deposition reduces the mean magnitude of the CMAQ low-bias in nitrate wet deposition with respect to National Atmospheric Deposition monitors to near zero. <br><br> Differences in urban/rural biases between model and satellite-retrieved NO<sub>2</sub> columns were examined to identify possible problems in model chemistry and/or transport. CMAQ columns were too large over urban areas. Biases at other locations were minor after accounting for the impacts of lightning-NO emissions and the averaging kernel on model columns. <br><br> In order to obtain an upper bound on the contribution of uncertainties in NO<sub>y</sub> chemistry to upper tropospheric NO<sub>x</sub> low biases, sensitivity calculations with updated chemistry were run for the time period of the Intercontinental Chemical Transport Experiment (INTEX-A) field campaign (summer 2004). After adjusting for possible interferences in NO<sub>2</sub> measurements and averaging over the entire campaign, these updates reduced 7–9 km biases from 32 to 17% and 9–12 km biases from 57 to 46%. While these changes lead to better agreement, a considerable unexplained NO<sub>2</sub> low-bias remains in the uppermost troposphere

Motives for choosing university and specialty applicants Ukraine (for example, students NTU "KPI")

Стаття присвячена аналізу портрета абітурієнта НТУ "ХПІ", де, крім визначення мотивів професійного вибору, самооцінки особистих здібностей, розглядаються і основні цілі навчання в НТУ "ХПІ". У статті підкреслюється, що проблема професійного самовизначення стояла перед молоддю завжди, а сьогодні вона особливо актуальна, тому що сучасні умови ринку праці ведуть до того, що більшість випускників не мають ясної життєвої перспективи. Аналізуються мотиви отримання абітурієнтами вищої освіти, мотивація вступу до НТУ "ХПІ", мотиви вибору спеціальності, період формування професійного інтересу. Проаналізовано мотиви професійного вибору абітурієнтів; період формування інтересу молодих людей до обраної спеціальності (процес самовизначення, коли здійснено вибір спеціальності, ВНЗ); специфіку вибору ВНЗ (яким за рахунком був ХПІ, хто допоміг визначитися з вибором); уявлення про процес навчання в університеті; відповідність уявлень про навчання в університеті реаліям університетського життя.This article analyzes portrait applicant NTU "KPI", where in addition to determining the reasons professional choice, self-personal skills, and discusses the main goals of training at NTU "KPI". The article highlights the problem of professional self -determination was always the youth, and today it is particularly urgent because the current labor market conditions lead to the fact that most graduates do not have a clear life perspective. Analyzed the motives of applicants receiving higher education, motivation admission to NTU "KPI" motives choice of specialty, during the formation of professional interest. Analyzed the motives of professional selection of applicants; during the formation of young people's interest to the chosen profession (the process of self-selection made when specialty Universities); the specific choice of university (how the account was HPI, who helped to choose); understanding of the learning process at the university; line ideas about university studies realities of university life

Systematic effects from an ambient-temperature, continuously-rotating half-wave plate

We present an evaluation of systematic effects associated with a continuously-rotating, ambient-temperature half-wave plate (HWP) based on two seasons of data from the Atacama B-Mode Search (ABS) experiment located in the Atacama Desert of Chile. The ABS experiment is a microwave telescope sensitive at 145 GHz. Here we present our in-field evaluation of celestial (CMB plus galactic foreground) temperature-to-polarization leakage. We decompose the leakage into scalar, dipole, and quadrupole leakage terms. We report a scalar leakage of ~0.01%, consistent with model expectations and an order of magnitude smaller than other CMB experiments have reported. No significant dipole or quadrupole terms are detected; we constrain each to be <0.07% (95% confidence), limited by statistical uncertainty in our measurement. Dipole and quadrupole leakage at this level lead to systematic error on r<0.01 before any mitigation due to scan cross-linking or boresight rotation. The measured scalar leakage and the theoretical level of dipole and quadrupole leakage produce systematic error of r<0.001 for the ABS survey and focal-plane layout before any data correction such as so-called deprojection. This demonstrates that ABS achieves significant beam systematic error mitigation from its HWP and shows the promise of continuously-rotating HWPs for future experiments.Comment: 11 pages, 8 figures; revision to submitted version, Fig. 5 and Eqs. (14) and (15) corrected; added Fig. 9 and description, text revisions for clarification, Fig. 5 revised for better calibration, corrected labeling errors and plotting bugs in Fig. 3, 4, and Eq. (14) and (15

The Diffusion of Humans and Cultures in the Course of the Spread of Farming

The most profound change in the relationship between humans and their environment was the introduction of agriculture and pastoralism. [....] For an understanding of the expansion process, it appears appropriate to apply a diffusive model. Broadly, these numerical modeling approaches can be catego- rized in correlative, continuous and discrete. Common to all approaches is the comparison to collections of radiocarbon data that show the apparent wave of advance of the transition to farming. However, these data sets differ in entry density and data quality. Often they disregard local and regional specifics and research gaps, or dating uncertainties. Thus, most of these data bases may only be used on a very general, broad scale. One of the pitfalls of using irregularly spaced or irregularly documented radiocarbon data becomes evident from the map generated by Fort (this volume, Chapter 16): while the general east-west and south-north trends become evident, some areas appear as having undergone anomalously early transitions to farming. This may be due to faulty entries into the data base or regional problems with radiocarbon dating, if not unnoticed or undocumented laboratory mistakes.Comment: 20 pages, 5 figures, submitted to Diffusive Spreading in Nature, Technology and Society, edited by Armin Bunde, J\"urgen Caro, J\"org K\"arger, Gero Vogl, Chapter 1

Characterizing Atacama B-mode Search Detectors with a Half-Wave Plate

The Atacama B-Mode Search (ABS) instrument is a cryogenic ($\sim$10 K) crossed-Dragone telescope located at an elevation of 5190 m in the Atacama Desert in Chile that observed for three seasons between February 2012 and October 2014. ABS observed the Cosmic Microwave Background (CMB) at large angular scales ($40<\ell<500$) to limit the B-mode polarization spectrum around the primordial B-mode peak from inflationary gravity waves at $\ell \sim100$. The ABS focal plane consists of 480 transition-edge sensor (TES) bolometers. They are coupled to orthogonal polarizations from a planar ortho-mode transducer (OMT) and observe at 145 GHz. ABS employs an ambient-temperature, rapidly rotating half-wave plate (HWP) to mitigate systematic effects and move the signal band away from atmospheric $1/f$ noise, allowing for the recovery of large angular scales. We discuss how the signal at the second harmonic of the HWP rotation frequency can be used for data selection and for monitoring the detector responsivities.Comment: 7 pages, 3 figures, conference proceedings submitted to the Journal of Low Temperature Detector

Modulation of CMB polarization with a warm rapidly-rotating half-wave plate on the Atacama B-Mode Search (ABS) instrument

We evaluate the modulation of Cosmic Microwave Background (CMB) polarization using a rapidly-rotating, half-wave plate (HWP) on the Atacama B-Mode Search (ABS). After demodulating the time-ordered-data (TOD), we find a significant reduction of atmospheric fluctuations. The demodulated TOD is stable on time scales of 500-1000 seconds, corresponding to frequencies of 1-2 mHz. This facilitates recovery of cosmological information at large angular scales, which are typically available only from balloon-borne or satellite experiments. This technique also achieves a sensitive measurement of celestial polarization without differencing the TOD of paired detectors sensitive to two orthogonal linear polarizations. This is the first demonstration of the ability to remove atmospheric contamination at these levels from a ground-based platform using a rapidly-rotating HWP.Comment: 8 pages, 8 figures, Published in RSI under the title "Modulation of cosmic microwave background polarization with a warm rapidly rotating half-wave plate on the Atacama B-Mode Search instrument.

Operation of a Bloch oscillator

We report the operation of a Bloch oscillator. The active medium was a staticvoltage driven, doped GaAs/AlAs superlattice which was electromagnetically coupled to a resonator. The oscillator produced tuneable microwave radiation (frequency ~ 60 GHz; power ~ 0.5 mW; efficiency ~ 4 %). The gain (~ 10^4 /cm) was due to the nonlinearity mediated by miniband electrons. We also present a theory of the oscillator. The Bloch oscillator should in principle be feasible for generation of radiation up to frequencies of 10 THz and more.Comment: 9 pages, 4 figure

Recovery of Large Angular Scale CMB Polarization for Instruments Employing Variable-delay Polarization Modulators

Variable-delay Polarization Modulators (VPMs) are currently being implemented in experiments designed to measure the polarization of the cosmic microwave background on large angular scales because of their capability for providing rapid, front-end polarization modulation and control over systematic errors. Despite the advantages provided by the VPM, it is important to identify and mitigate any time-varying effects that leak into the synchronously modulated component of the signal. In this paper, the effect of emission from a $300$ K VPM on the system performance is considered and addressed. Though instrument design can greatly reduce the influence of modulated VPM emission, some residual modulated signal is expected. VPM emission is treated in the presence of rotational misalignments and temperature variation. Simulations of time-ordered data are used to evaluate the effect of these residual errors on the power spectrum. The analysis and modeling in this paper guides experimentalists on the critical aspects of observations using VPMs as front-end modulators. By implementing the characterizations and controls as described, front-end VPM modulation can be very powerful for mitigating $1/f$ noise in large angular scale polarimetric surveys. None of the systematic errors studied fundamentally limit the detection and characterization of B-modes on large scales for a tensor-to-scalar ratio of $r=0.01$. Indeed, $r<0.01$ is achievable with commensurately improved characterizations and controls.Comment: 13 pages, 13 figures, 1 table, matches published versio