10 research outputs found
Microarray Method for the Rapid Detection of Glycosaminoglycan–Protein Interactions
Glycosaminoglycans (GAGs) perform numerous vital functions within the body. As major components of
the extracellular matrix, these polysaccharides participate in a diverse array of cell-signaling events. We have
developed a simple microarray assay for the evaluation of protein binding to various GAG subclasses. In a
single experiment, the binding to all members of the GAG family can be rapidly determined, giving insight
into the relative specificity of the interactions and the importance of specific sulfation motifs. The arrays
are facile to prepare from commercially available materials
Optimization of fermentation parameters for enhanced production of naringinase by soil isolate Aspergillus niger VB07
Optimization of process parameters for naringinase production by Aspergillus tubingensis
Statistical based media optimization and production of naringinase using Aspergillus brasiliensis 1344
Heparin octasaccharide decoy liposomes inhibit replication of multiple viruses
Heparan sulfate (HS) is a ubiquitous glycosaminoglycan that serves as a cellular attachment site for a number of significant human pathogens, including respiratory syncytial virus (RSV), human parainfluenza virus 3 (hPIV3), and herpes simplex virus (HSV). Decoy receptors can target pathogens by binding to the receptor pocket on viral attachment proteins, acting as ‘molecular sinks’ and preventing the pathogen from binding to susceptible host cells. Decoy receptors functionalized with HS could bind to pathogens and prevent infection, so we generated decoy liposomes displaying HS-octasaccharide (HS-octa). These decoy liposomes significantly inhibited RSV, hPIV3, and HSV infectivity in vitro to a greater degree than the original HS-octa building block. The degree of inhibition correlated with the density of HS-octa displayed on the liposome surface. Decoy liposomes with HS-octa inhibited infection of viruses to a greater extent than either full-length heparin or HS-octa alone. Decoy liposomes were effective when added prior to infection or following the initial infection of cells in vitro. By targeting the well-conserved receptor-binding sites of HS-binding viruses, decoy liposomes functionalized with HS-octa are a promising therapeutic antiviral agent and illustrate the utility of the liposome delivery platform
Updates on naringinase : structural and biotechnological aspects
Naringinases has attracted a great deal of attention in recent years due to its hydrolytic activities which include the production of rhamnose, and prunin and debittering of citrus fruit juices. While this enzyme is widely distributed in fungi, its production from bacterial sources is less commonly known. Fungal naringinase are very important as they are used industrially in large amounts and have been extensively studied during the past decade. In this article, production of bacterial naringinase and potential biotechnological applications are discussed. Bacterial rhamnosidases are exotype enzymes that hydrolyse terminal non-reducing α-l-rhamnosyl groups from α-l-rhamnose containing polysaccharides and glycosides. Structurally, they are classified into family 78 of glycoside hydrolases and characterized by the presence of Asp567 and Glu841 in their active site. Optimization of fermentation conditions and enzyme engineering will allow the development of improved rhamnosidases for advancing suggested industrial applications.<br /
Structure and immunogenicity of pre-fusion-stabilized human metapneumovirus F glycoprotein
An efficient vaccine for human metapneumovirus (hMPV) will likely rely on neutralizing antibodies against the fusion protein (F). Here, the authors determine the crystal structure of pre-fusion-stabilized hMPV F and identify a dense glycan shield that affects generation of neutralizing antibodies