AUTOTEM - Automated geometry meshing and heat conduction calculation

Temperature distribution for an arbitrary irregular body is calculated by AUTOTEM, which generates required input data automatically by computer. Temperature distribution is calculated for a two-dimensional plane section in /x,y/ coordinates or for an axisymmetric irregular body in /r,z/ coordinates

Temporal markers of prosodic boundaries in children's speech production

It is often thought that the ability to use prosodic features accurately is mastered in early childhood. However, research to date has produced conflicting evidence, notably about the development of children's ability to mark prosodic boundaries. This paper investigates (i) whether, by the age of eight, children use temporal boundary features in their speech in a systematic way, and (ii) to what extent adult listeners are able to interpret their production accurately and unambiguously. The material consists of minimal pairs of utterances: one utterance includes a compound noun, in which there is no prosodic boundary after the first noun, e.g. ‘coffee-cake and tea’, while the other utterance includes simple nouns, separated by a prosodic boundary, e.g. ‘coffee, cake and tea’. Ten eight-year-old children took part, and their productions were rated by 23 adult listeners. Two phonetic exponents of prosodic boundaries were analysed: pause duration and phrase-final lengthening. The results suggest that, at the age of 8, there is considerable variability among children in their ability to mark phrase boundaries of the kind analysed in the experiment, with some children failing to differentiate between the members of the minimal pairs reliably. The differences between the children in their use of boundary features were reflected in the adults' perceptual judgements. Both temporal cues to prosodic boundaries significantly affected the perceptual ratings, with pause being a more salient determinant of ratings than phrase-final lengthening

Propeller propulsion integration, phase 1

A bibliography was compiled of all readily available sources of propeller analytical and experimental studies conducted during the 1930 through 1960 period. A propeller test stand was developed for the measurement of thrust and torque characteristics of full scale general aviation propellers and installed in the LaRC 30 x 60 foot full scale wind tunnel. A tunnel entry was made during the January through February 1980 period. Several propellers were tested, but unforseen difficulties with the shaft thrust torque balance severely degraded the data quality

Rigidity analysis of HIV-1 protease

We present a rigidity analysis on a large number of X-ray crystal structures of the enzyme HIV-1 protease using the 'pebble game' algorithm of the software FIRST. We find that although the rigidity profile remains similar across a comprehensive set of high resolution structures, the profile changes significantly in the presence of an inhibitor. Our study shows that the action of the inhibitors is to restrict the flexibility of the beta-hairpin flaps which allow access to the active site. The results are discussed in the context of full molecular dynamics simulations as well as data from NMR experiments.Comment: 4 pages, 3 figures. Conference proceedings for CMMP conference 2010 which was held at the University of Warwic

A study of possible sea state information in the sample and hold gate statistics for the GEOS-3 satellite altimeter

The statistical variations in the sample gate outputs of the GEOS-3 satellite altimeter were studied for possible sea state information. After examination of a large number of statistical characteristics of the altimeter waveforms, it was found that the best sea predictor for H-1/3 in the range of 0 to 3 meters was the 75th percentile of sample and hold gate number 11

How much dystrophin is enough: the physiological consequences of different levels of dystrophin in the mdx mouse

Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle function is poorly understood. In order to robustly evaluate functional improvement, we used in situ protocols in the mdx mouse to measure muscle strength and resistance to eccentric contraction-induced damage. Here, we modelled the treatment of muscle with pre-existing dystrophic pathology using antisense oligonucleotides conjugated to a cell-penetrating peptide. We reveal that 15% homogeneous dystrophin expression is sufficient to protect against eccentric contraction-induced injury. In addition, we demonstrate a >40% increase in specific isometric force following repeated administrations. Strikingly, we show that changes in muscle strength are proportional to dystrophin expression levels. These data define the dystrophin restoration levels required to slow down or prevent disease progression and improve overall muscle function once a dystrophic environment has been established in the mdx mouse model

Local Structure and It's Effect on The Ferromagnetic Properties of La0.5_{0.5}Sr0.5_{0.5}CoO3_3 thin films}

We have used high-resolution Extended X-ray Absorption Fine-Structure and diffraction techniques to measure the local structure of strained La$_{0.5}$Sr$_{0.5}$CoO$_3$ films under compression and tension. The lattice mismatch strain in these compounds affects both the bond lengths and the bond angles, though the larger effect on the bandwidth is due to the bond length changes. The popular double exchange model for ferromagnetism in these compounds provides a correct qualitative description of the changes in Curie temperature $T_C$, but quantitatively underestimates the changes. A microscopic model for ferromagnetism that provides a much stronger dependence on the structural distortions is needed.Comment: 4 pages, 4 figure

Cmah-dystrophin deficient mdx mice display an accelerated cardiac phenotype that is improved following peptide-PMO exon skipping treatment

Duchenne muscular dystrophy (DMD) is caused by loss of dystrophin protein, leading to progressive muscle weakness and premature death due to respiratory and/or cardiac complications. Cardiac involvement is characterized by progressive dilated cardiomyopathy, decreased fractional shortening and metabolic dysfunction involving reduced metabolism of fatty acids—the major cardiac metabolic substrate. Several mouse models have been developed to study molecular and pathological consequences of dystrophin deficiency, but do not recapitulate all aspects of human disease pathology and exhibit a mild cardiac phenotype. Here we demonstrate that Cmah (cytidine monophosphate-sialic acid hydroxylase)-deficient mdx mice (Cmah−/−;mdx) have an accelerated cardiac phenotype compared to the established mdx model. Cmah−/−;mdx mice display earlier functional deterioration, specifically a reduction in right ventricle (RV) ejection fraction and stroke volume (SV) at 12 weeks of age and decreased left ventricle diastolic volume with subsequent reduced SV compared to mdx mice by 24 weeks. They further show earlier elevation of cardiac damage markers for fibrosis (Ctgf), oxidative damage (Nox4) and haemodynamic load (Nppa). Cardiac metabolic substrate requirement was assessed using hyperpolarized magnetic resonance spectroscopy indicating increased in vivo glycolytic flux in Cmah−/−;mdx mice. Early upregulation of mitochondrial genes (Ucp3 and Cpt1) and downregulation of key glycolytic genes (Pdk1, Pdk4, Ppara), also denote disturbed cardiac metabolism and shift towards glucose utilization in Cmah−/−;mdx mice. Moreover, we show long-term treatment with peptide-conjugated exon skipping antisense oligonucleotides (20-week regimen), resulted in 20% cardiac dystrophin protein restoration and significantly improved RV cardiac function. Therefore, Cmah−/−;mdx mice represent an appropriate model for evaluating cardiac benefit of novel DMD therapeutics