Sharing a different voice: Attending to stories in collaborative writing

Through three stories, we hope to reveal how sometimes contradictory or unrecognizable aspects of our lives, selves, and stories can create tensions in the collaborative writing endeavor. We begin with a story that illuminates some of the narrative tensions that surface during a decade of writing collaboratively. In an effort to navigate these tensions, we explore two further stories in dialogue as a way to reveal how dominant narratives shape our lives and the stories we might tell. One aim of sharing these stories is to reveal how problematic ways of being are often inseparable from one’s cultural legacy. Making previously obscured narratives visible paves the way for imaginary leaps that are necessary for change. We hope these insights are useful for other writers and collaborators and those who seek caring, responsive, and nurturing writing relationships yet realize this journey can be problematic

Circulating microRNAs miR-331 and miR-195 differentiate local luminal a from metastatic breast cancer

BACKGROUND: Breast cancer is the leading cause of cancer related death in women, with metastasis the principle cause of mortality. New non-invasive prognostic markers are needed for the early detection of metastasis, facilitating treatment decision optimisation. MicroRNA (miRNA) are small, non-coding RNAs regulating gene expression and involved in many cellular processes, including metastasis. As biomarkers, circulating miRNAs (in blood) hold great promise for informing diagnosis or monitoring treatment responses. METHODS: Plasma extracted RNA from age matched local Luminal A (n = 4) or metastatic disease (n = 4) were profiled using Next Generation Sequencing. Selected differentially expressed miRNA were validated on a whole blood extracted miRNA cohort [distant metastatic disease (n = 22), local disease (n = 31), healthy controls (n = 21)]. Area Under the Curve (AUC) in Receiver Operating Characteristic (ROC) analyses was performed. RESULTS: Of 4 miRNA targets tested (miR-181a, miR-329, miR-331, miR-195), mir-331 was significantly over-expressed in patients with metastatic disease, compared to patients with local disease (p \u3c 0.001) or healthy controls (p \u3c 0.001). miR-195 was significantly under-expressed in patients with metastatic disease, compared to patients with local disease (p \u3c 0.001) or healthy controls (p = 0.043). In combination, miR-331 and miR-195 produced an AUC of 0.902, distinguishing metastatic from local breast cancer. CONCLUSIONS: We identified and validated two circulating miRNAs differentiating local Luminal A breast cancers from metastatic breast cancers. Further investigation will reveal the molecular role of these miRNAs in metastasis, and determine if they are subtype specific. This work demonstrates the ability of circulating miRNA to identify metastatic disease, and potentially inform diagnosis or treatment effectiveness

A Cellular Automata Model for Citrus Variagated Chlorosis

A cellular automata model is proposed to analyze the progress of Citrus Variegated Chlorosis epidemics in S\~ao Paulo oranges plantation. In this model epidemiological and environmental features, such as motility of sharpshooter vectors which perform L\'evy flights, hydric and nutritional level of plant stress and seasonal climatic effects, are included. The observed epidemics data were quantitatively reproduced by the proposed model varying the parameters controlling vectors motility, plant stress and initial population of diseased plants.Comment: 10 pages, 10 figures, Scheduled tentatively for the issue of: 01Nov0

Evolution of Testing Algorithms at a University Hospital for Detection of Clostridium difficile Infections

We present the evolution of testing algorithms at our institution in which the C. Diff Quik Chek Complete immunochromatographic cartridge assay determines the presence of both glutamate dehydrogenase and Clostridium difficile toxins A and B as a primary screen for C. difficile infection and indeterminate results (glutamate dehydrogenase positive, toxin A and B negative) are confirmed by the GeneXpert C. difficile PCR assay. This two-step algorithm is a cost-effective method for highly sensitive detection of toxigenic C. difficile

Gender and educational leadership in England: a comparison of secondary headteachers' views over time

In the context of gender being a barrier to accessing leadership, this paper presents a comparison of the views of men and women head teacher (principals) of secondary schools in England in the 1990s and in 2004. The same survey instrument was used on both occasions. The perceptions of the head teachers show change in some areas and no change in others. Overall, women are more likely to become head teachers and are now less likely to be categorised into pastoral roles, but in some cases women still meet prejudice from governors and others in the wider community. Women head teachers are more likely to have partners and children than in the 1990s, sharing equally or carrying most of the domestic responsibilities, whereas male colleagues are most likely to have partners who take the majority of responsibility in the home. Essentialist stereotypes about women and men as leaders still prevail, although both the women and men head teachers see themselves as adopting a traditionally ‘feminine’ style of leadership. Women head teachers are likely to see some benefits in being a woman in a role stereotypically associated with men. However, there has been an increase in the proportion of women who feel that they have to prove their worth as a leader, and this may be linked with increased levels of accountability in schools

The A-ring reduction of 11-ketotestosterone is efficiently catalysed by AKR1D1 and SRD5A2 but not SRD5A1

Testosterone and its 5α-reduced form, 5α-dihydrotestosterone, were previously thought to represent the only active androgens in humans. However, recent studies have shown that the potent androgen, 11-ketotestosterone, derived from the adrenal androgen precursor, 11β-hydroxyandrostenedione, may in fact serve as the primary androgen in healthy women. Yet, despite recent renewed interest in these steroids, their downstream metabolism has remained undetermined. We therefore set out to investigate the metabolism of 11-ketotestosterone by characterising the 5α- or 5β-reduction commitment step. We show that inactivation of 11-ketotestosterone is predominantly driven by AKR1D1, which efficiently catalyses the 5β-reduction of 11-ketotestosterone, committing it to a metabolic pathway that terminates in 11-ketoetiocholanolone. We demonstrate that 5α-reduction of 11-ketotestosterone is catalysed by SRD5A2, but not SRD5A1, and terminates in 11-ketoandrosterone, but is only responsible for a minority of 11-ketotestosterone inactivation. However, as 11-ketoetiocholanolone is also generated by the metabolism of the glucocorticoid cortisone, 11-ketoandrosterone should be considered a more specific urinary marker of 11-ketotestosterone production

Joining the conspiracy? Negotiating ethics and emotions in researching (around) AIDS in southern Africa

AIDS is an emotive subject, particularly in southern Africa. Among those who have been directly affected by the disease, or who perceive themselves to be personally at risk, talking about AIDS inevitably arouses strong emotions - amongst them fear, distress, loss and anger. Conventionally, human geography research has avoided engagement with such emotions. Although the ideal of the detached observer has been roundly critiqued, the emphasis in methodological literature on 'doing no harm' has led even qualitative researchers to avoid difficult emotional encounters. Nonetheless, research is inevitably shaped by emotions, not least those of the researchers themselves. In this paper, we examine the role of emotions in the research process through our experiences of researching the lives of 'Young AIDS migrants' in Malawi and Lesotho. We explore how the context of the research gave rise to the production of particular emotions, and how, in response, we shaped the research, presenting a research agenda focused more on migration than AIDS. This example reveals a tension between universalised ethics expressed through ethical research guidelines that demand informed consent, and ethics of care, sensitive to emotional context. It also demonstrates how dualistic distinctions between reason and emotion, justice and care, global and local are unhelpful in interpreting the ethics of research practice

Exploring the nature and synchronicity of early cluster formation in the Large Magellanic Cloud - IV. Evidence for multiple populations in Hodge 11 and NGC 2210

We present a multiple population search in two old Large Magellanic Cloud (LMC) Globular clusters, Hodge 11 and NGC 2210. This work uses data from the Advanced Camera for Surveys and Wide Field Camera 3 on the Hubble Space Telescope from programme GO- 14164 in Cycle 23. Both of these clusters exhibit a broadened main sequence with the second population representing (20±~5) per cent forNGC2210 and (30±~5) per cent forHodge 11. In both clusters, the smaller population is redder than the primary population, suggesting CNO variations. Hodge 11 also displays a bluer second population in the horizontal branch, which is evidence for helium enhancement. However, even though NGC 2210 shows similarities to Hodge 11 in the main sequence, there does not appear to be a second population on NGC 2210's horizontal branch. This is the first photometric evidence that ancient LMC Globular clusters exhibit multiple stellar populations.Fil: Gilligan, Christina K.. Dartmouth College; Estados UnidosFil: Chaboyer, Brian. Dartmouth College; Estados UnidosFil: Cummings, Jeffrey D.. University Johns Hopkins; Estados UnidosFil: Mackey, Dougal. Australian National University. Research School of Astronomy & Astrophysics; AustraliaFil: Cohen, Roger E.. Space Telescope Science Institute; Estados UnidosFil: Geisler, Douglas. Universidad de La Serena; ChileFil: Grocholski, Aaron J.. University of Washington; Estados UnidosFil: Parisi, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Astronomía Teórica y Experimental. Universidad Nacional de Córdoba. Observatorio Astronómico de Córdoba. Instituto de Astronomía Teórica y Experimental; ArgentinaFil: Sarajedini, Ata. University of Florida; Estados UnidosFil: Ventura, Paolo. Osservatorio Astronomico Di Roma; ItaliaFil: Villanova, Sandro. Universidad de Concepción; ChileFil: Yang, Soung-Chul. Korea Astronomy And Space Science Institute; Corea del SurFil: Wagner-Kaiser, Rachel. University of Florida; Estados Unido

Exploring the nature and synchronicity of early cluster formation in the Large Magellanic Cloud - V. Multiple populations in ancient globular clusters

We examine four ancient LargeMagellanic Cloud (LMC) globular clusters (GCs) for evidence of multiple stellar populations using the Advanced Camera for Surveys andWide Field Camera 3 on the Hubble Space Telescope Programme GO-14164. NGC 1466, NGC 1841, and NGC 2257 all show evidence for a redder, secondary population along themain sequence. Reticulum does not showevidence for the presence of a redder population, but thisGChas the least number of stars and Monte Carlo simulations indicate that the sample of main-sequence stars is too small to robustly infer whether a redder population exists in this cluster. The second, redder, population of the other three clusters constitutes ∼ 30 - 40 per cent of the total population along the main sequence. This brings the total number of ancient LMC GCs with known split or broadened main sequences to five. However, unlike for Hodge 11 and NGC 2210 (see Gilligan et al. (2019)), none of the clusters shows evidence for multiple populations in the horizontal branch. We also do not find evidence of a second population along the red giant branch.Fil: Gilligan, Christina K.. Dartmouth College; Estados UnidosFil: Chaboyer, Brian. Dartmouth College; Estados UnidosFil: Cummings, Jeffrey D.. University Johns Hopkins; Estados UnidosFil: Mackey, Dougal. Australian National University; AustraliaFil: Cohen, Roger E.. Space Telescope Science Institute; Estados UnidosFil: Geisler, Douglas. Universidad de Concepción; ChileFil: Grocholski, Aaron J.. University of Florida; Estados UnidosFil: Parisi, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Astronomía Teórica y Experimental. Universidad Nacional de Córdoba. Observatorio Astronómico de Córdoba. Instituto de Astronomía Teórica y Experimental; ArgentinaFil: Sarajedini, Ata. University of Florida; Estados UnidosFil: Ventura, Paolo. Osservatorio Astronomico Di Roma; ItaliaFil: Villanova, Sandro. Universidad de Concepción; ChileFil: Yang, Soung-Chul. Korea Astronomy And Space Science Institute; Corea del SurFil: Wagner-Kaiser, Rachel. University of Florida; Estados Unido

Young People, Biographical Narratives and the Life Grid: Young People's Accounts of Parental Substance Use

Research into potentially sensitive issues with young people presents numerous methodological and ethical challenges. While recent studies have highlighted the advantages of task-based activities in research with young people, the literature on life history research provides few suggestions as to effective and appropriate research tools for encouraging young people to tell their stories. This paper explores the contribution that may be made to such research by the life grid, a visual tool for mapping important life events against the passage of time and prompting wide-ranging discussion. Critical advantages of the life grid in qualitative research include: its visual element which can help to engage interviewer and interviewee in a process of constructing and reflecting on a concrete life history record; its role in creating a more relaxed research encounter supportive of the respondent’s ‘voice’; and facilitating the discussion of sensitive issues. In addition, the way in which use of the grid anchors such narratives in accounts of everyday life, often revealing interesting tensions, is explored. These points are discussed with reference to an exploratory study of young people’s experience of parental substance use