CLOT PROMOTING AND DISSOLVING PROPERTIES OF CUCUMBER (CUCUMIS SATIVUS) SAP, VALIDATING ITS USE IN TRADITIONAL MEDICINE

Objective: To investigate the biochemical events that are associated with the skin softening, cleansing and wound healing properties of the cucumber (Cucumis sativus L) sap extract.Methods: Preparation of cucumber sap extract (CSE). Assay of CSE for proteolytic activity, plasma re-calcification time, APTT, PT, thrombin-like activity, plasmin-like activity, and effect on platelet aggregation and wound healing property by physical, biochemical and histological examinations. Appropriate positive and negative controls were maintained wherever necessary.Results: CSE decreased the plasma re-calcification time and prothrombin time (PT) and showed factor VII (pro-convertin) like activity. EGTA or EDTA pre-treated CSE did not alter the plasma recalcification time and PT. CSE readily hydrolyzed the plasma clot and azocasein; while, IAA pre-treated CSE did not hydrolyze the plasma clot and azocasein. CSE inhibited the agonists collagen, ADP and epinephrine induced platelet aggregation in PRP in the order epinephrine>collagen>ADP with the respective IC50 of 22 ± 2.5, 20 ± 3 and 11 ± 2 µg/ml. PMSF pre-treated but not IAA and EDTA pre-treated CSE lost the platelet aggregation inhibition property. Further, CSE augmented wound healing process including the scar removal in a mouse model. The SOD, CAT, GSH activities and hydroxyproline, hexosamine and hexuronic acid contents were increased while, NO, LPO and MPO activities were decreased compared to control values. Histological study revealed accelerated wound healing involving epithelialisation and re-formation of skin following CSE treatment compared to Neosporin.Conclusion: CSE contain metallo-, serine and cysteine proteases, and interfere in clot formation, dissolution and wound healing process, which validates the use of cucumber as cosmetics and to treat wounds by traditional healers.Â

Fishery, biology and population characteristics of longtail tuna, Thunnus tonggol (Bleeker, 1851) caught along the Indian coast

The longtail tuna, Thunnus tonggol, Bleeker, 1851 is an economically important species from commercial and recreational point of view. The species inhabit shelf and oceanic waters of tropical and temperate regions of the Indo-Pacific between 47° N and 33° S (Froese and Pauly, 2009) and generally occupy neritic areas of the oceans close to land masses (Yesaki, 1994)

Status of exploitation of coastal tunas in the Indian seas

Tuna and billfish production from Indian seas during the period 1985-'99 evinced an increasing trend and the landings fluctuated between 24,287 t (1987) and 53,6621 (1992). The average annual production during the said period was 40,2041, contributing to 3.6% of the total pelagic fish landings and 1.8% of the total marine fish landings. On an average, 24% of the total tuna and billfish production during 1991-'99 was contributed by northwest coast, 2% by Andaman and Nicobar islands and 14.9% by Lakshadweep. Among the maritime states, Kerala (36 %), Gujarat (18.1%) Tamilnadu (11.6%), Maharashtra (5.9%), Kamataka (5%), Andhra Pradesh (4.4 %) and Goa (2%) were the prime tuna producing states

Stock assessment of coastal tunas in the Indian seas

Tuna and billfish production from the Indian coastal waters, Lakshadweep and Andaman and Nicobar islands during the period 1985-'99 averaged 40,204 t. The contribution by E.affinis, Athazard, T.tonggol, K.pelamis and T.albacares (young ones) were 18,5041,6,8521,3,093 t, 3,3921 and 2,2111 respectively. Drift gill net was the major gear employed in the coastal tuna fishery. The length frequency data collected on the above species during 1990-'98 at seven centres along the Indian coast were analysed employing FiSAT programme to estimate their growth and mortality parameters, exploitation rates and relative yield per recmit

Present status of exploitation of fish and shellfish resources: Tunas and billfishes

Despite the fact that several communications in the past have dealt with the trend of tuna fishery at different centres along the west coast of India, no directed attempt has been made till date to study the tuna fishery during the southwest monsoon period along this coast and to synthesise its problems and prospects. The present mmmunication deals with their trend, general fishery, craft and gear employed in the fishing, fishing grounds, seasonal variation in catch, effort and catch rate and characteristics during premonsoon, monsoon and post seasons. In addition, the species composition and length composition of major species during different seams and available information of the spawning biology of tunas along the west coast of India are also dealt with. The effect of tuna fishing. demand and price structure during monsoon period as cornpad to other spasons and the management meas- are also presented and discussed

Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica

Due to poor diagnostic facilities and a lack of medical alertness, allergy to Vespa wasps may be underestimated. Few allergens have been identified from Vespa wasps

Progressive hemorrhage and myotoxicity induced by echis carinatus venom in murine model: neutralization by inhibitor cocktail of n,n,n `,n `-tetrakis (2-pyridylmethyl) ethane-1,2-diamine and silymarin

Viperbite is often associated with severe local toxicity, including progressive hemorrhage and myotoxicity, persistent even after the administration of anti-snake venom (ASV). In the recent past, investigations have revealed the orchestrated actions of Zn2+ metalloproteases (Zn(2+)MPs), phospholipase A(2)s (PLA(2)s) and hyaluronidases (HYs) in the onset and progression of local toxicity from the bitten site. As a consequence, venom researchers and medical practitioners are in deliberate quest of potent molecules alongside ASV to tackle the brutal local manifestations induced by aforesaid venom toxins. Based on these facts, we have demonstrated the protective efficacy of inhibitor cocktail containing equal ratios of N,N,N', N'-tetrakis (2-pyridylmethyl) ethane-1,2-diamine (TPEN) and silymarin (SLN) against progressive local toxicity induced by Echis carinatus venom (ECV). In our previous study we have shown the inhibitory potentials of TPEN towards Zn(2+)MPs of ECV (IC50: 6.7 mu M). In this study we have evaluated in vitro inhibitory potentials of SLN towards PLA(2)s (IC50: 12.5 mu M) and HYs (IC50: 8 mu M) of ECV in addition to docking studies. Further, we have demonstrated the protection of ECV induced local toxicity with 10 mM inhibitor cocktail following 15, 30 min (for hemorrhage and myotoxicity); 60 min (for hemorrhage alone) of ECV injection in murine model. The histological examination of skin and thigh muscle sections taken out from the site of ECV injection substantiated the overall protection offered by inhibitor cocktail. In conclusion, the protective efficacy of inhibitor cocktail is of high interest and can be administered locally alongside ASV to treat severe local toxicity

Hyaluronidase inhibitors: a biological and therapeutic perspective

The hyaluronidases (HAases) are a group of less extensively studied glycosidases distributed throughout the animal kingdom and are popularly known as `spreading factors'. In recent years, HAases received much attention due to their ability to abruptly alter the hyaluronic acid (HA) homeostasis. HAases preferentially degrade HA, which is a megadalton acidic structural polysaccharide found exclusively in the extracellular matrix (ECM) of animals. The HA-HAase system has been suggested to participate in many pathophysiological conditions. The HA degradation in ECM, crack down the structural integrity with an eventual increased tissue permeability that is attributed for the spreading property. The spreading property has been widely accepted in functions including envenomation, acrosomal reaction/ovum fertilization, cancer progression, microbial pathogenesis such as wound infections, pneumonia, and other sepses like, bacteremia and meningitis. HA fragmentation has dual effects; generation of a wide molecular range bioactive oligosaccharides of angiogenic, pro-inflammatory, and immunostimulatory properties; and impairment in the reservoir capacity of ECM that holds metal ions, growth factors, cytokines and various enzymes for signal transduction. Hence, inhibition of HA degradation appears critical and imperative in HAase mediated pathological conditions. HAase inhibitors are thus potent regulators that maintain HA homeostasis and they might serve as anti-inflammatory, anti-aging, anti-microbial, anticancer and anti-venom/toxin and contraceptive agents. In addition, HAase inhibitors may serve as tools to understand several unexplained and complex functions of HAases in HA metabolism. Therefore, this review is expected to provide an integrated update as of 2008 on the HAase inhibitors and their possible role as therapeutics in the management of a wide range of pathological conditions

Snake venom hyaluronidase: An evidence for isoforms and extracellular matrix degradation

The present study attempts to establish the isoforms of hyaluronidase enzyme and their possible role in the spreading of toxins during envenomation. Screening of venoms of 15 snakes belonging to three different families revealed varied hyaluronidase activity in ELISA-like assay, but with relatively similar pH and temperature optima. The zymograms of individual venoms showed varied activity banding patterns and indicated the presence of at least two molecular forms of the enzyme. During envenomation, activity of hyaluronidase is considered crucial for the spreading of toxins and is presumed to distort the integrity of extracellular matrix through the degradation of hyaluronic acid in it. This property has been addressed through localization of hyaluronic acid in human skin and muscle tissue sections using the probe, biotinylated hyaluronic acid binding protein. Faint and discontinuous staining pattern of hyaluronidase treated tissue sections over intense staining of untreated tissue sections confirm the selective degradation of hyaluronic acid in extracellular matrix and thus provide an evidence for the spreading property of the enzyme

The metalloprotease, NN-PF3 from Naja naja venom inhibits platelet aggregation primarily by affecting alpha 2 beta 1 integrin

NN-PF3 is a non-toxic, anticoagulant, high-molecular-mass (67.81 kDa) metalloprotease from Indian cobra (Naja naja) venom. In the present study, NN-PF3 was investigated for the mechanism of inhibition of collagen-induced aggregation of human platelets. The complete inhibition of collagen-induced aggregation and partial inhibition of ADP-and epinephrine-induced aggregation has the respective IC(50) of 75 +/- 5, 185 +/- 10, and 232 +/- 12 nM, whereas no inhibition of thrombin-, arachidonic acid-, and ristocetin-induced aggregation of platelets was observed in platelet-rich plasma. Further, native NN-PF3 and EDTA-inactivated NN-PF3 inhibited collagen-induced aggregation of washed platelets with respective IC(50) of 75 +/- 4 and 180 +/- 6 nM. The higher inhibitory effect of native NN-PF3 compared with EDTA-inactivated NN-PF3 suggests the enzymatic and non-enzymatic mechanism of inhibition. NN-PF3 pretreatment affected the collagen binding but not the fibrinogen, and fibronectin binding of washed platelets in adhesion assay suggested that the collagen receptors are affected. Western blot study using anti-integrin alpha 2 beta 1 mAb 6F1 suggested that NN-PF3 binds to integrin alpha 2 beta 1 in a primary structure-dependent manner only and is not cleaved. There was a drastic reduction in the intensity of several intracellular signaling phosphotyrosine protein bands when monoclonal anti-phosphotyrosine antibody was used, suggesting that the major activation pathway of platelets get affected, which occurs through glycoprotein VI. NN-PF3 did not bind to collagen as revealed by Western blot using anti-collagen mAb. Furthermore, neither the proteolytic cleavage of fibrinogen nor its degradation products by NN-PF3 contributed for the collagen-induced platelet aggregation inhibition